Evaluated gene expressions of Metallo beta lactamase genes GIM and , VIM, SPM in Pseudomonas aeruginosa clinical isolates
Pseudomonas aeruginosa is considered as one of the human health care problems, P. aeruginosa’s carbapenem resistance emerges by several different mechanisms, some of which include carbapenems genes. P. aeruginosa’s carbapenem resistance is a significant health concern, So this study aims to evaluate...
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| Veröffentlicht in: | Molecular biology reports 2023-12, Vol.50 (12), p.10111-10120 |
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| description | Pseudomonas aeruginosa
is considered as one of the human health care problems,
P. aeruginosa’s
carbapenem resistance emerges by several different mechanisms, some of which include carbapenems genes.
P. aeruginosa’s
carbapenem resistance is a significant health concern, So this study aims to evaluate MBL gene expressions. The study was conducted at the Department of Microbiology, AL-Mahmoodia Hospital, over one year from January to December 2022. The samples were collected from patients with different clinical sources (Burn, Urine, Wound, Sputum, Ear, and Blood), from different ages while. Samples were collected from three hospitals in Baghdad including Al-Yarmouk Teaching Hospital, AL-Mahmmodiya Hospital, and Child’s Central Teaching Hospital. A study analyzed 55
P. aeruginosa
strains from various clinical sources, the study utilizes the chemical characterization, VITEK 2 system,
16s rRNA
, antibiogram sensitivity tests, antibiotic susceptibility using eight antibiotics, including Amikacin, Ciprofloxacin, Levofloxacin, Imipenem Meropenem, Piperacillin, Cefepim and Aztreonam. The test of bacterial susceptibility revealed that each isolate was highly resistant to piperacillin, which are 96.36%, and lower resistance to Ciprofloxacin, which are 32%. Phenotypic screening carbapenem resistance methods combined the disk synergy test and conventional PCR that were used to detect isolates by using
16 S rRNA.
This proves that the bacteria is
P. aeruginosa
and computed by measuring gene expression of the target genes (
GIM, VIM, SPM)
by using the real-time PCR, which is employed for twenty-five isolates. The result indicates that the expression level of the
VIM
gene is highly regulated in carbapenem-resistance isolates compared to control isolates that is 1.00. While the expression level of gene
GIM
and
SPM
is downregulated in carbapenem-resistance isolates compared to control isolates that is 6. The carbapenem
VIM
and
GIM
, SPM (class B) genes are essential for resistance in
P. aeruginosa
induced by chromosomal changes that modify membrane permeability efflux pump overexpression for genes. As a result, many studies require for discovering new strategies to reduce the threat to public health through preventing the spread of these isolates via tight infections, control measures, and the reduction of the danger to public health. |
| doi_str_mv | 10.1007/s11033-023-08883-7 |
| format | Article |
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is considered as one of the human health care problems,
P. aeruginosa’s
carbapenem resistance emerges by several different mechanisms, some of which include carbapenems genes.
P. aeruginosa’s
carbapenem resistance is a significant health concern, So this study aims to evaluate MBL gene expressions. The study was conducted at the Department of Microbiology, AL-Mahmoodia Hospital, over one year from January to December 2022. The samples were collected from patients with different clinical sources (Burn, Urine, Wound, Sputum, Ear, and Blood), from different ages while. Samples were collected from three hospitals in Baghdad including Al-Yarmouk Teaching Hospital, AL-Mahmmodiya Hospital, and Child’s Central Teaching Hospital. A study analyzed 55
P. aeruginosa
strains from various clinical sources, the study utilizes the chemical characterization, VITEK 2 system,
16s rRNA
, antibiogram sensitivity tests, antibiotic susceptibility using eight antibiotics, including Amikacin, Ciprofloxacin, Levofloxacin, Imipenem Meropenem, Piperacillin, Cefepim and Aztreonam. The test of bacterial susceptibility revealed that each isolate was highly resistant to piperacillin, which are 96.36%, and lower resistance to Ciprofloxacin, which are 32%. Phenotypic screening carbapenem resistance methods combined the disk synergy test and conventional PCR that were used to detect isolates by using
16 S rRNA.
This proves that the bacteria is
P. aeruginosa
and computed by measuring gene expression of the target genes (
GIM, VIM, SPM)
by using the real-time PCR, which is employed for twenty-five isolates. The result indicates that the expression level of the
VIM
gene is highly regulated in carbapenem-resistance isolates compared to control isolates that is 1.00. While the expression level of gene
GIM
and
SPM
is downregulated in carbapenem-resistance isolates compared to control isolates that is 6. The carbapenem
VIM
and
GIM
, SPM (class B) genes are essential for resistance in
P. aeruginosa
induced by chromosomal changes that modify membrane permeability efflux pump overexpression for genes. As a result, many studies require for discovering new strategies to reduce the threat to public health through preventing the spread of these isolates via tight infections, control measures, and the reduction of the danger to public health.</description><identifier>ISSN: 0301-4851</identifier><identifier>EISSN: 1573-4978</identifier><identifier>DOI: 10.1007/s11033-023-08883-7</identifier><identifier>PMID: 37917414</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Amikacin ; Animal Anatomy ; Animal Biochemistry ; Anti-Bacterial Agents - pharmacology ; antibiotic resistance ; Antibiotics ; Aztreonam ; beta-Lactamases - genetics ; beta-Lactamases - metabolism ; Biomedical and Life Sciences ; blood ; Carbapenems ; Carbapenems - pharmacology ; Child ; children ; Ciprofloxacin ; class ; Clinical isolates ; Gene Expression ; Genes ; health services ; Histology ; hospitals ; human health ; Humans ; Imipenem ; Levofloxacin ; Life Sciences ; Membrane permeability ; Meropenem ; Metallo-β-lactamase ; Microbial Sensitivity Tests ; microbiology ; Morphology ; Original Article ; phenotype ; Piperacillin ; Pseudomonas aeruginosa ; Pseudomonas aeruginosa - genetics ; Pseudomonas Infections - genetics ; Pseudomonas Infections - microbiology ; Public health ; quantitative polymerase chain reaction ; Real-Time Polymerase Chain Reaction ; RNA, Ribosomal, 16S ; rRNA 16S ; Sputum ; Teaching hospitals ; transporters ; urine</subject><ispartof>Molecular biology reports, 2023-12, Vol.50 (12), p.10111-10120</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2023. The Author(s), under exclusive licence to Springer Nature B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c408t-a9b4bfef53c19f26e7f092d101fb8cd1b61f0e3fc32decdfbb22de446a8424a13</citedby><cites>FETCH-LOGICAL-c408t-a9b4bfef53c19f26e7f092d101fb8cd1b61f0e3fc32decdfbb22de446a8424a13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11033-023-08883-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11033-023-08883-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27915,27916,41479,42548,51310</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37917414$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ali, Marwa Ghalib</creatorcontrib><creatorcontrib>Almoneim, Zahraa Abd</creatorcontrib><creatorcontrib>Kareem, Sawsan M.</creatorcontrib><title>Evaluated gene expressions of Metallo beta lactamase genes GIM and , VIM, SPM in Pseudomonas aeruginosa clinical isolates</title><title>Molecular biology reports</title><addtitle>Mol Biol Rep</addtitle><addtitle>Mol Biol Rep</addtitle><description>Pseudomonas aeruginosa
is considered as one of the human health care problems,
P. aeruginosa’s
carbapenem resistance emerges by several different mechanisms, some of which include carbapenems genes.
P. aeruginosa’s
carbapenem resistance is a significant health concern, So this study aims to evaluate MBL gene expressions. The study was conducted at the Department of Microbiology, AL-Mahmoodia Hospital, over one year from January to December 2022. The samples were collected from patients with different clinical sources (Burn, Urine, Wound, Sputum, Ear, and Blood), from different ages while. Samples were collected from three hospitals in Baghdad including Al-Yarmouk Teaching Hospital, AL-Mahmmodiya Hospital, and Child’s Central Teaching Hospital. A study analyzed 55
P. aeruginosa
strains from various clinical sources, the study utilizes the chemical characterization, VITEK 2 system,
16s rRNA
, antibiogram sensitivity tests, antibiotic susceptibility using eight antibiotics, including Amikacin, Ciprofloxacin, Levofloxacin, Imipenem Meropenem, Piperacillin, Cefepim and Aztreonam. The test of bacterial susceptibility revealed that each isolate was highly resistant to piperacillin, which are 96.36%, and lower resistance to Ciprofloxacin, which are 32%. Phenotypic screening carbapenem resistance methods combined the disk synergy test and conventional PCR that were used to detect isolates by using
16 S rRNA.
This proves that the bacteria is
P. aeruginosa
and computed by measuring gene expression of the target genes (
GIM, VIM, SPM)
by using the real-time PCR, which is employed for twenty-five isolates. The result indicates that the expression level of the
VIM
gene is highly regulated in carbapenem-resistance isolates compared to control isolates that is 1.00. While the expression level of gene
GIM
and
SPM
is downregulated in carbapenem-resistance isolates compared to control isolates that is 6. The carbapenem
VIM
and
GIM
, SPM (class B) genes are essential for resistance in
P. aeruginosa
induced by chromosomal changes that modify membrane permeability efflux pump overexpression for genes. As a result, many studies require for discovering new strategies to reduce the threat to public health through preventing the spread of these isolates via tight infections, control measures, and the reduction of the danger to public health.</description><subject>Amikacin</subject><subject>Animal Anatomy</subject><subject>Animal Biochemistry</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>antibiotic resistance</subject><subject>Antibiotics</subject><subject>Aztreonam</subject><subject>beta-Lactamases - genetics</subject><subject>beta-Lactamases - metabolism</subject><subject>Biomedical and Life Sciences</subject><subject>blood</subject><subject>Carbapenems</subject><subject>Carbapenems - pharmacology</subject><subject>Child</subject><subject>children</subject><subject>Ciprofloxacin</subject><subject>class</subject><subject>Clinical isolates</subject><subject>Gene Expression</subject><subject>Genes</subject><subject>health services</subject><subject>Histology</subject><subject>hospitals</subject><subject>human health</subject><subject>Humans</subject><subject>Imipenem</subject><subject>Levofloxacin</subject><subject>Life Sciences</subject><subject>Membrane permeability</subject><subject>Meropenem</subject><subject>Metallo-β-lactamase</subject><subject>Microbial Sensitivity Tests</subject><subject>microbiology</subject><subject>Morphology</subject><subject>Original Article</subject><subject>phenotype</subject><subject>Piperacillin</subject><subject>Pseudomonas aeruginosa</subject><subject>Pseudomonas aeruginosa - genetics</subject><subject>Pseudomonas Infections - genetics</subject><subject>Pseudomonas Infections - microbiology</subject><subject>Public health</subject><subject>quantitative polymerase chain reaction</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>RNA, Ribosomal, 16S</subject><subject>rRNA 16S</subject><subject>Sputum</subject><subject>Teaching hospitals</subject><subject>transporters</subject><subject>urine</subject><issn>0301-4851</issn><issn>1573-4978</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkc9u1DAQxi0EokvhBTggS1w4NGDHTuwcUdWWlbpqJf5cLccZr1I59uJJEPRpeBaeDNNti8SBHqzP0vzmG818hLzk7C1nTL1DzpkQFavL01qLSj0iK94oUclO6cdkxQTjldQNPyDPEK8YY5Kr5ik5EKrjSnK5Itcn32xY7AwD3UIECt93GRDHFJEmTzcw2xAS7YvSYN1sJ4twg-Kvn2frDbVxoEf0y3pzRD9ebugY6SXCMqQpRYvUQl62Y0xoqQtjHJ0NdMQUykB8Tp54GxBe3Ooh-Xx68un4Q3V-cbY-fn9eOcn0XNmul70H3wjHO1-3oDzr6oEz7nvtBt633DMQ3ol6ADf4vq_LR8rWallLy8UhebP33eX0dQGczTSigxBshLSgEbwRjWJtkYfQWutW1G0xLujrf9CrtORYFilUJ6Qqt5eFqveUywkxgze7PE42_zCcmT8hmn2IpoRobkI0qjS9urVe-gmG-5a71Aog9gCWUtxC_jv7P7a_AZJzqDA</recordid><startdate>20231201</startdate><enddate>20231201</enddate><creator>Ali, Marwa Ghalib</creator><creator>Almoneim, Zahraa Abd</creator><creator>Kareem, Sawsan M.</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20231201</creationdate><title>Evaluated gene expressions of Metallo beta lactamase genes GIM and , VIM, SPM in Pseudomonas aeruginosa clinical isolates</title><author>Ali, Marwa Ghalib ; Almoneim, Zahraa Abd ; Kareem, Sawsan M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-a9b4bfef53c19f26e7f092d101fb8cd1b61f0e3fc32decdfbb22de446a8424a13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Amikacin</topic><topic>Animal Anatomy</topic><topic>Animal Biochemistry</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>antibiotic resistance</topic><topic>Antibiotics</topic><topic>Aztreonam</topic><topic>beta-Lactamases - genetics</topic><topic>beta-Lactamases - metabolism</topic><topic>Biomedical and Life Sciences</topic><topic>blood</topic><topic>Carbapenems</topic><topic>Carbapenems - pharmacology</topic><topic>Child</topic><topic>children</topic><topic>Ciprofloxacin</topic><topic>class</topic><topic>Clinical isolates</topic><topic>Gene Expression</topic><topic>Genes</topic><topic>health services</topic><topic>Histology</topic><topic>hospitals</topic><topic>human health</topic><topic>Humans</topic><topic>Imipenem</topic><topic>Levofloxacin</topic><topic>Life Sciences</topic><topic>Membrane permeability</topic><topic>Meropenem</topic><topic>Metallo-β-lactamase</topic><topic>Microbial Sensitivity Tests</topic><topic>microbiology</topic><topic>Morphology</topic><topic>Original Article</topic><topic>phenotype</topic><topic>Piperacillin</topic><topic>Pseudomonas aeruginosa</topic><topic>Pseudomonas aeruginosa - genetics</topic><topic>Pseudomonas Infections - genetics</topic><topic>Pseudomonas Infections - microbiology</topic><topic>Public health</topic><topic>quantitative polymerase chain reaction</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>RNA, Ribosomal, 16S</topic><topic>rRNA 16S</topic><topic>Sputum</topic><topic>Teaching hospitals</topic><topic>transporters</topic><topic>urine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ali, Marwa Ghalib</creatorcontrib><creatorcontrib>Almoneim, Zahraa Abd</creatorcontrib><creatorcontrib>Kareem, Sawsan M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Molecular biology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ali, Marwa Ghalib</au><au>Almoneim, Zahraa Abd</au><au>Kareem, Sawsan M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluated gene expressions of Metallo beta lactamase genes GIM and , VIM, SPM in Pseudomonas aeruginosa clinical isolates</atitle><jtitle>Molecular biology reports</jtitle><stitle>Mol Biol Rep</stitle><addtitle>Mol Biol Rep</addtitle><date>2023-12-01</date><risdate>2023</risdate><volume>50</volume><issue>12</issue><spage>10111</spage><epage>10120</epage><pages>10111-10120</pages><issn>0301-4851</issn><eissn>1573-4978</eissn><abstract>Pseudomonas aeruginosa
is considered as one of the human health care problems,
P. aeruginosa’s
carbapenem resistance emerges by several different mechanisms, some of which include carbapenems genes.
P. aeruginosa’s
carbapenem resistance is a significant health concern, So this study aims to evaluate MBL gene expressions. The study was conducted at the Department of Microbiology, AL-Mahmoodia Hospital, over one year from January to December 2022. The samples were collected from patients with different clinical sources (Burn, Urine, Wound, Sputum, Ear, and Blood), from different ages while. Samples were collected from three hospitals in Baghdad including Al-Yarmouk Teaching Hospital, AL-Mahmmodiya Hospital, and Child’s Central Teaching Hospital. A study analyzed 55
P. aeruginosa
strains from various clinical sources, the study utilizes the chemical characterization, VITEK 2 system,
16s rRNA
, antibiogram sensitivity tests, antibiotic susceptibility using eight antibiotics, including Amikacin, Ciprofloxacin, Levofloxacin, Imipenem Meropenem, Piperacillin, Cefepim and Aztreonam. The test of bacterial susceptibility revealed that each isolate was highly resistant to piperacillin, which are 96.36%, and lower resistance to Ciprofloxacin, which are 32%. Phenotypic screening carbapenem resistance methods combined the disk synergy test and conventional PCR that were used to detect isolates by using
16 S rRNA.
This proves that the bacteria is
P. aeruginosa
and computed by measuring gene expression of the target genes (
GIM, VIM, SPM)
by using the real-time PCR, which is employed for twenty-five isolates. The result indicates that the expression level of the
VIM
gene is highly regulated in carbapenem-resistance isolates compared to control isolates that is 1.00. While the expression level of gene
GIM
and
SPM
is downregulated in carbapenem-resistance isolates compared to control isolates that is 6. The carbapenem
VIM
and
GIM
, SPM (class B) genes are essential for resistance in
P. aeruginosa
induced by chromosomal changes that modify membrane permeability efflux pump overexpression for genes. As a result, many studies require for discovering new strategies to reduce the threat to public health through preventing the spread of these isolates via tight infections, control measures, and the reduction of the danger to public health.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>37917414</pmid><doi>10.1007/s11033-023-08883-7</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
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| subjects | Amikacin Animal Anatomy Animal Biochemistry Anti-Bacterial Agents - pharmacology antibiotic resistance Antibiotics Aztreonam beta-Lactamases - genetics beta-Lactamases - metabolism Biomedical and Life Sciences blood Carbapenems Carbapenems - pharmacology Child children Ciprofloxacin class Clinical isolates Gene Expression Genes health services Histology hospitals human health Humans Imipenem Levofloxacin Life Sciences Membrane permeability Meropenem Metallo-β-lactamase Microbial Sensitivity Tests microbiology Morphology Original Article phenotype Piperacillin Pseudomonas aeruginosa Pseudomonas aeruginosa - genetics Pseudomonas Infections - genetics Pseudomonas Infections - microbiology Public health quantitative polymerase chain reaction Real-Time Polymerase Chain Reaction RNA, Ribosomal, 16S rRNA 16S Sputum Teaching hospitals transporters urine |
| title | Evaluated gene expressions of Metallo beta lactamase genes GIM and , VIM, SPM in Pseudomonas aeruginosa clinical isolates |
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