Synthesis and characterization of chitosan-zinc-salicylic acid nanoparticles: A plant biostimulant

The vastly expanding global population raised the demand for profuse food grain production. For food security in India, high yield and nutritional quality of grain crops, both are essential. Zinc is a crucial micronutrient generally deficient in food grains grown in India, reflecting their deteriorating nutritional quality. To address these issues, in the present study, a novel tri-component nanoparticle of chitosan‑zinc-salicylic acid (CS-Zn-SA NPs) has been synthesized by ionotropic gelation method. The average size of synthesized CS-Zn-SA NPs was recorded 13.5 nm by dynamic light scattering (DLS) spectroscopy. The presence of chitosan, zinc and salicylic acid and crosslinking among these components in synthesized nanoparticles has been demonstrated by Fourier transforms infrared (FTIR) spectroscopy and thermogravimetric analysis (TGA). Further, synthesized CS-Zn-SA NPs at various concentrations (50–200 ppm) were evaluated for seed germination via seed priming, yield, grain zinc content and defence enzyme activity through the foliar application. CS-Zn-SA NPs revealed significant seed germination activities, 19.8 % higher grain yield, 45.5 % increased grain zinc content and manyfold defence enzyme activities than the control. The obtained results exposed the potential of CS-Zn-SA NPs as a stimulant for effective seedling development, higher yield, a virtuous micronutrient fortifying agent and defence enzyme promoter. •A new and novel biopolymer-metal-plant growth regulator-based nanoparticle of chitosan‑zinc-salicylic acid (CS-Zn-SA NPs) has been synthesized by ionotropic gelation method.•The synergistic effect of chitosan, zinc and salicylic acid in CS-Zn-SA nanoparticles stimulated effective seed germination and promoted the plant yield in wheat plants.•Moreover, CS-Zn-SA NPs also trigged grain zinc content accumulation and improved defence enzyme activities that indicated its effectiveness under stressed conditions.