Deciphering the mechanism of anti‐quorum sensing post‐biotic mediators against Streptococcus mutans
Objective Glucosyltransferases (Gtfs) and quorum sensing (QS) mediated transduction genes play critical roles in the pathogenesis of Streptococcus mutan‐mediated dental caries. Therefore, targeting gtfs and QS‐mediated virulence genes have therefore emerged as an intriguing goal for efficient therap...
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| Veröffentlicht in: | Oral diseases 2024-07, Vol.30 (5), p.3471-3479 |
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| creator | Pallavi, Preeti Kumar, Vikas Sen, Sudip Kumar Raut, Sangeeta |
| description | Objective
Glucosyltransferases (Gtfs) and quorum sensing (QS) mediated transduction genes play critical roles in the pathogenesis of Streptococcus mutan‐mediated dental caries. Therefore, targeting gtfs and QS‐mediated virulence genes have therefore emerged as an intriguing goal for efficient therapeutic approaches that block cariogenic biofilms.
Methods
Post‐biotic mediators (PMs) obtained from our previously isolated and characterized beneficial bacteria Enterobacter colacae PS‐74 was assessed for its antibiofilm potential against S. mutans. According to the transcriptome method, qRT‐PCR analysis was performed against virulence genes. For microscopic visualization, SEM and CLSM analyses were used to confirm the inhibitory effects of PMs.
Results
PMs dramatically reduced the expression of QS signal transduction, glucan metabolism, and biofilm‐regulated genes such gtfB, gtfC, ComDE, VicR, brpA in S. mutans, which validates the outcomes of in vitro result. Their unique metabolites may help to control biofilm formation by eluding antimicrobial resistance.
Conclusion
Considering the above findings, PMs may deem to be an innovative, alluring, and secure method for preventing dental caries due to their biological activity. Our study unravels the inhibitory effect of PMs, which will contribute to instruct drug design strategies for effective inhibition of S. mutans biofilms. |
| doi_str_mv | 10.1111/odi.14780 |
| format | Article |
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Glucosyltransferases (Gtfs) and quorum sensing (QS) mediated transduction genes play critical roles in the pathogenesis of Streptococcus mutan‐mediated dental caries. Therefore, targeting gtfs and QS‐mediated virulence genes have therefore emerged as an intriguing goal for efficient therapeutic approaches that block cariogenic biofilms.
Methods
Post‐biotic mediators (PMs) obtained from our previously isolated and characterized beneficial bacteria Enterobacter colacae PS‐74 was assessed for its antibiofilm potential against S. mutans. According to the transcriptome method, qRT‐PCR analysis was performed against virulence genes. For microscopic visualization, SEM and CLSM analyses were used to confirm the inhibitory effects of PMs.
Results
PMs dramatically reduced the expression of QS signal transduction, glucan metabolism, and biofilm‐regulated genes such gtfB, gtfC, ComDE, VicR, brpA in S. mutans, which validates the outcomes of in vitro result. Their unique metabolites may help to control biofilm formation by eluding antimicrobial resistance.
Conclusion
Considering the above findings, PMs may deem to be an innovative, alluring, and secure method for preventing dental caries due to their biological activity. Our study unravels the inhibitory effect of PMs, which will contribute to instruct drug design strategies for effective inhibition of S. mutans biofilms.</description><identifier>ISSN: 1354-523X</identifier><identifier>ISSN: 1601-0825</identifier><identifier>EISSN: 1601-0825</identifier><identifier>DOI: 10.1111/odi.14780</identifier><identifier>PMID: 37870077</identifier><language>eng</language><publisher>Denmark: Wiley Subscription Services, Inc</publisher><subject>Anti-Bacterial Agents - pharmacology ; antibiofilm ; Antimicrobial resistance ; Bacterial Proteins - metabolism ; Biofilms ; Biofilms - drug effects ; Biological activity ; cell‐free supernatant ; Dental caries ; Dental Caries - microbiology ; Dental Caries - prevention & control ; Drug development ; Glucans - metabolism ; Glucosyltransferases - antagonists & inhibitors ; Glucosyltransferases - metabolism ; post‐biotic mediators ; Quorum sensing ; Quorum Sensing - drug effects ; Signal transduction ; Streptococcus mutans ; Streptococcus mutans - drug effects ; Transcriptomes ; Virulence ; Virulence Factors ; virulence genes</subject><ispartof>Oral diseases, 2024-07, Vol.30 (5), p.3471-3479</ispartof><rights>2023 Wiley Periodicals LLC.</rights><rights>2024 Wiley Periodicals LLC.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3130-25d1db78ba21e022ec2e69f1def305db39e529f1eb40f490f6999afe3d17fca23</cites><orcidid>0000-0001-7922-6464</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fodi.14780$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fodi.14780$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,782,786,1419,27933,27934,45583,45584</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37870077$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pallavi, Preeti</creatorcontrib><creatorcontrib>Kumar, Vikas</creatorcontrib><creatorcontrib>Sen, Sudip Kumar</creatorcontrib><creatorcontrib>Raut, Sangeeta</creatorcontrib><title>Deciphering the mechanism of anti‐quorum sensing post‐biotic mediators against Streptococcus mutans</title><title>Oral diseases</title><addtitle>Oral Dis</addtitle><description>Objective
Glucosyltransferases (Gtfs) and quorum sensing (QS) mediated transduction genes play critical roles in the pathogenesis of Streptococcus mutan‐mediated dental caries. Therefore, targeting gtfs and QS‐mediated virulence genes have therefore emerged as an intriguing goal for efficient therapeutic approaches that block cariogenic biofilms.
Methods
Post‐biotic mediators (PMs) obtained from our previously isolated and characterized beneficial bacteria Enterobacter colacae PS‐74 was assessed for its antibiofilm potential against S. mutans. According to the transcriptome method, qRT‐PCR analysis was performed against virulence genes. For microscopic visualization, SEM and CLSM analyses were used to confirm the inhibitory effects of PMs.
Results
PMs dramatically reduced the expression of QS signal transduction, glucan metabolism, and biofilm‐regulated genes such gtfB, gtfC, ComDE, VicR, brpA in S. mutans, which validates the outcomes of in vitro result. Their unique metabolites may help to control biofilm formation by eluding antimicrobial resistance.
Conclusion
Considering the above findings, PMs may deem to be an innovative, alluring, and secure method for preventing dental caries due to their biological activity. Our study unravels the inhibitory effect of PMs, which will contribute to instruct drug design strategies for effective inhibition of S. mutans biofilms.</description><subject>Anti-Bacterial Agents - pharmacology</subject><subject>antibiofilm</subject><subject>Antimicrobial resistance</subject><subject>Bacterial Proteins - metabolism</subject><subject>Biofilms</subject><subject>Biofilms - drug effects</subject><subject>Biological activity</subject><subject>cell‐free supernatant</subject><subject>Dental caries</subject><subject>Dental Caries - microbiology</subject><subject>Dental Caries - prevention & control</subject><subject>Drug development</subject><subject>Glucans - metabolism</subject><subject>Glucosyltransferases - antagonists & inhibitors</subject><subject>Glucosyltransferases - metabolism</subject><subject>post‐biotic mediators</subject><subject>Quorum sensing</subject><subject>Quorum Sensing - drug effects</subject><subject>Signal transduction</subject><subject>Streptococcus mutans</subject><subject>Streptococcus mutans - drug effects</subject><subject>Transcriptomes</subject><subject>Virulence</subject><subject>Virulence Factors</subject><subject>virulence genes</subject><issn>1354-523X</issn><issn>1601-0825</issn><issn>1601-0825</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kMtKAzEUQIMovhf-gAy40cW0ecw0M0tpfRQEFyq4C5nMTZvSmYxJBunOT_Ab_RJTqy4Es0luODmEg9AJwQMS19DWZkAyXuAttE9GmKS4oPl2PLM8S3PKnvfQgfcLjAkvGd1Fe4wXHGPO99FsAsp0c3CmnSVhDkkDai5b45vE6kS2wXy8vb_01vVN4qH1a6yzPsTbythgVHxQGxms84mcSdP6kDwEB12wyirV-6Tpg2z9EdrRcunh-Hs_RE_XV4_j2_Tu_mY6vrxLFSMMpzSvSV3xopKUAKYUFIVRqUkNmuG8rlgJOY0zVBnWWYn1qCxLqYHVhGslKTtE5xtv5-xLDz6IxngFy6VswfZe0KKIcShhWUTP_qAL27s2_k6wyPBRdPNIXWwo5az3DrTonGmkWwmCxbq-iPXFV_3Inn4b-ypm-SV_ckdguAFezRJW_5vE_WS6UX4CbXSSIA</recordid><startdate>202407</startdate><enddate>202407</enddate><creator>Pallavi, Preeti</creator><creator>Kumar, Vikas</creator><creator>Sen, Sudip Kumar</creator><creator>Raut, Sangeeta</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-7922-6464</orcidid></search><sort><creationdate>202407</creationdate><title>Deciphering the mechanism of anti‐quorum sensing post‐biotic mediators against Streptococcus mutans</title><author>Pallavi, Preeti ; Kumar, Vikas ; Sen, Sudip Kumar ; Raut, Sangeeta</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3130-25d1db78ba21e022ec2e69f1def305db39e529f1eb40f490f6999afe3d17fca23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Anti-Bacterial Agents - pharmacology</topic><topic>antibiofilm</topic><topic>Antimicrobial resistance</topic><topic>Bacterial Proteins - metabolism</topic><topic>Biofilms</topic><topic>Biofilms - drug effects</topic><topic>Biological activity</topic><topic>cell‐free supernatant</topic><topic>Dental caries</topic><topic>Dental Caries - microbiology</topic><topic>Dental Caries - prevention & control</topic><topic>Drug development</topic><topic>Glucans - metabolism</topic><topic>Glucosyltransferases - antagonists & inhibitors</topic><topic>Glucosyltransferases - metabolism</topic><topic>post‐biotic mediators</topic><topic>Quorum sensing</topic><topic>Quorum Sensing - drug effects</topic><topic>Signal transduction</topic><topic>Streptococcus mutans</topic><topic>Streptococcus mutans - drug effects</topic><topic>Transcriptomes</topic><topic>Virulence</topic><topic>Virulence Factors</topic><topic>virulence genes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pallavi, Preeti</creatorcontrib><creatorcontrib>Kumar, Vikas</creatorcontrib><creatorcontrib>Sen, Sudip Kumar</creatorcontrib><creatorcontrib>Raut, Sangeeta</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Oral diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pallavi, Preeti</au><au>Kumar, Vikas</au><au>Sen, Sudip Kumar</au><au>Raut, Sangeeta</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Deciphering the mechanism of anti‐quorum sensing post‐biotic mediators against Streptococcus mutans</atitle><jtitle>Oral diseases</jtitle><addtitle>Oral Dis</addtitle><date>2024-07</date><risdate>2024</risdate><volume>30</volume><issue>5</issue><spage>3471</spage><epage>3479</epage><pages>3471-3479</pages><issn>1354-523X</issn><issn>1601-0825</issn><eissn>1601-0825</eissn><abstract>Objective
Glucosyltransferases (Gtfs) and quorum sensing (QS) mediated transduction genes play critical roles in the pathogenesis of Streptococcus mutan‐mediated dental caries. Therefore, targeting gtfs and QS‐mediated virulence genes have therefore emerged as an intriguing goal for efficient therapeutic approaches that block cariogenic biofilms.
Methods
Post‐biotic mediators (PMs) obtained from our previously isolated and characterized beneficial bacteria Enterobacter colacae PS‐74 was assessed for its antibiofilm potential against S. mutans. According to the transcriptome method, qRT‐PCR analysis was performed against virulence genes. For microscopic visualization, SEM and CLSM analyses were used to confirm the inhibitory effects of PMs.
Results
PMs dramatically reduced the expression of QS signal transduction, glucan metabolism, and biofilm‐regulated genes such gtfB, gtfC, ComDE, VicR, brpA in S. mutans, which validates the outcomes of in vitro result. Their unique metabolites may help to control biofilm formation by eluding antimicrobial resistance.
Conclusion
Considering the above findings, PMs may deem to be an innovative, alluring, and secure method for preventing dental caries due to their biological activity. Our study unravels the inhibitory effect of PMs, which will contribute to instruct drug design strategies for effective inhibition of S. mutans biofilms.</abstract><cop>Denmark</cop><pub>Wiley Subscription Services, Inc</pub><pmid>37870077</pmid><doi>10.1111/odi.14780</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0001-7922-6464</orcidid></addata></record> |
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| subjects | Anti-Bacterial Agents - pharmacology antibiofilm Antimicrobial resistance Bacterial Proteins - metabolism Biofilms Biofilms - drug effects Biological activity cell‐free supernatant Dental caries Dental Caries - microbiology Dental Caries - prevention & control Drug development Glucans - metabolism Glucosyltransferases - antagonists & inhibitors Glucosyltransferases - metabolism post‐biotic mediators Quorum sensing Quorum Sensing - drug effects Signal transduction Streptococcus mutans Streptococcus mutans - drug effects Transcriptomes Virulence Virulence Factors virulence genes |
| title | Deciphering the mechanism of anti‐quorum sensing post‐biotic mediators against Streptococcus mutans |
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