Label-free protein recognition using an aptamer-based impedance measurement assay

Label-free protein recognition using an aptamer-based impedance measurement assay

A novel aptamer-based biosensing assay for label-free protein detection and quantification by measuring the change in electrochemical impedance upon protein–aptamer complex formation was demonstrated. Our process starts with a self-assembly of the DNA aptamer on a microfabricated thin film gold elec...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Sensors and actuators. B, Chemical Chemical, 2006-03, Vol.114 (1), p.433-437
Hauptverfasser: Cai, Hong, Lee, Thomas Ming-Hung, Hsing, I-Ming
Format: Artikel
Sprache:eng
Schlagworte:
Aptamer
Electrochemical impedance spectroscopy
Label-free protein detection
Thrombin
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 437
container_issue 1
container_start_page 433
container_title Sensors and actuators. B, Chemical
container_volume 114
creator Cai, Hong
Lee, Thomas Ming-Hung
Hsing, I-Ming
description A novel aptamer-based biosensing assay for label-free protein detection and quantification by measuring the change in electrochemical impedance upon protein–aptamer complex formation was demonstrated. Our process starts with a self-assembly of the DNA aptamer on a microfabricated thin film gold electrode, followed by the recognition of the protein binding event via monitoring the interfacial electron transfer resistance with electrochemical impedance spectroscopy. The thrombin binding aptamer (TBA) has high binding affinity and specificity to its target protein and the impedance detection assay shows a reliable and sensitive quantification of thrombin with a detection range of four orders of magnitude and a detection limit of 0.1 nM.
doi_str_mv 10.1016/j.snb.2005.06.017
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_28787853</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0925400505005460</els_id><sourcerecordid>28787853</sourcerecordid><originalsourceid>FETCH-LOGICAL-c359t-54363c0bb5742360f10e2093430b08469b513316e538a51af400db6432ab72523</originalsourceid><addsrcrecordid>eNqFkE9LxDAQxYMouK5-AG85eWudJE3T4kkW_8GCCHoOSTtdsrRpTVrBb2-W9azMYQ7z3sybHyHXDHIGrLzd59HbnAPIHMocmDohK1YpkQlQ6pSsoOYyK9L4nFzEuAeAQpSwIm9bY7HPuoBIpzDO6DwN2Iw772Y3erpE53fUeGqm2QwYMmsittQNE7bGN0gHNHEJOKCfqYnRfF-Ss870Ea9--5p8PD68b56z7evTy-Z-mzVC1nMm033RgLVSFTxF6Rggh1oUAixURVlbyYRgJUpRGclMl8K3tiwEN1ZxycWa3Bz3ptifC8ZZDy422PfG47hEzSuVSop_hawuuGJMJiE7Cpswxhiw01NwgwnfmoE-UNZ7nSjrA2UNpU6Uk-fu6MH06pfDoGPjMIFpXcI463Z0f7h_AMuhg9g</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>19427115</pqid></control><display><type>article</type><title>Label-free protein recognition using an aptamer-based impedance measurement assay</title><source>Elsevier ScienceDirect Journals Complete</source><creator>Cai, Hong ; Lee, Thomas Ming-Hung ; Hsing, I-Ming</creator><creatorcontrib>Cai, Hong ; Lee, Thomas Ming-Hung ; Hsing, I-Ming</creatorcontrib><description>A novel aptamer-based biosensing assay for label-free protein detection and quantification by measuring the change in electrochemical impedance upon protein–aptamer complex formation was demonstrated. Our process starts with a self-assembly of the DNA aptamer on a microfabricated thin film gold electrode, followed by the recognition of the protein binding event via monitoring the interfacial electron transfer resistance with electrochemical impedance spectroscopy. The thrombin binding aptamer (TBA) has high binding affinity and specificity to its target protein and the impedance detection assay shows a reliable and sensitive quantification of thrombin with a detection range of four orders of magnitude and a detection limit of 0.1 nM.</description><identifier>ISSN: 0925-4005</identifier><identifier>EISSN: 1873-3077</identifier><identifier>DOI: 10.1016/j.snb.2005.06.017</identifier><language>eng</language><publisher>Elsevier B.V</publisher><subject>Aptamer ; Electrochemical impedance spectroscopy ; Label-free protein detection ; Thrombin</subject><ispartof>Sensors and actuators. B, Chemical, 2006-03, Vol.114 (1), p.433-437</ispartof><rights>2005 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c359t-54363c0bb5742360f10e2093430b08469b513316e538a51af400db6432ab72523</citedby><cites>FETCH-LOGICAL-c359t-54363c0bb5742360f10e2093430b08469b513316e538a51af400db6432ab72523</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0925400505005460$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids></links><search><creatorcontrib>Cai, Hong</creatorcontrib><creatorcontrib>Lee, Thomas Ming-Hung</creatorcontrib><creatorcontrib>Hsing, I-Ming</creatorcontrib><title>Label-free protein recognition using an aptamer-based impedance measurement assay</title><title>Sensors and actuators. B, Chemical</title><description>A novel aptamer-based biosensing assay for label-free protein detection and quantification by measuring the change in electrochemical impedance upon protein–aptamer complex formation was demonstrated. Our process starts with a self-assembly of the DNA aptamer on a microfabricated thin film gold electrode, followed by the recognition of the protein binding event via monitoring the interfacial electron transfer resistance with electrochemical impedance spectroscopy. The thrombin binding aptamer (TBA) has high binding affinity and specificity to its target protein and the impedance detection assay shows a reliable and sensitive quantification of thrombin with a detection range of four orders of magnitude and a detection limit of 0.1 nM.</description><subject>Aptamer</subject><subject>Electrochemical impedance spectroscopy</subject><subject>Label-free protein detection</subject><subject>Thrombin</subject><issn>0925-4005</issn><issn>1873-3077</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNqFkE9LxDAQxYMouK5-AG85eWudJE3T4kkW_8GCCHoOSTtdsrRpTVrBb2-W9azMYQ7z3sybHyHXDHIGrLzd59HbnAPIHMocmDohK1YpkQlQ6pSsoOYyK9L4nFzEuAeAQpSwIm9bY7HPuoBIpzDO6DwN2Iw772Y3erpE53fUeGqm2QwYMmsittQNE7bGN0gHNHEJOKCfqYnRfF-Ss870Ea9--5p8PD68b56z7evTy-Z-mzVC1nMm033RgLVSFTxF6Rggh1oUAixURVlbyYRgJUpRGclMl8K3tiwEN1ZxycWa3Bz3ptifC8ZZDy422PfG47hEzSuVSop_hawuuGJMJiE7Cpswxhiw01NwgwnfmoE-UNZ7nSjrA2UNpU6Uk-fu6MH06pfDoGPjMIFpXcI463Z0f7h_AMuhg9g</recordid><startdate>20060330</startdate><enddate>20060330</enddate><creator>Cai, Hong</creator><creator>Lee, Thomas Ming-Hung</creator><creator>Hsing, I-Ming</creator><general>Elsevier B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7U5</scope><scope>8BQ</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>20060330</creationdate><title>Label-free protein recognition using an aptamer-based impedance measurement assay</title><author>Cai, Hong ; Lee, Thomas Ming-Hung ; Hsing, I-Ming</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c359t-54363c0bb5742360f10e2093430b08469b513316e538a51af400db6432ab72523</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Aptamer</topic><topic>Electrochemical impedance spectroscopy</topic><topic>Label-free protein detection</topic><topic>Thrombin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cai, Hong</creatorcontrib><creatorcontrib>Lee, Thomas Ming-Hung</creatorcontrib><creatorcontrib>Hsing, I-Ming</creatorcontrib><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Sensors and actuators. B, Chemical</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cai, Hong</au><au>Lee, Thomas Ming-Hung</au><au>Hsing, I-Ming</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Label-free protein recognition using an aptamer-based impedance measurement assay</atitle><jtitle>Sensors and actuators. B, Chemical</jtitle><date>2006-03-30</date><risdate>2006</risdate><volume>114</volume><issue>1</issue><spage>433</spage><epage>437</epage><pages>433-437</pages><issn>0925-4005</issn><eissn>1873-3077</eissn><abstract>A novel aptamer-based biosensing assay for label-free protein detection and quantification by measuring the change in electrochemical impedance upon protein–aptamer complex formation was demonstrated. Our process starts with a self-assembly of the DNA aptamer on a microfabricated thin film gold electrode, followed by the recognition of the protein binding event via monitoring the interfacial electron transfer resistance with electrochemical impedance spectroscopy. The thrombin binding aptamer (TBA) has high binding affinity and specificity to its target protein and the impedance detection assay shows a reliable and sensitive quantification of thrombin with a detection range of four orders of magnitude and a detection limit of 0.1 nM.</abstract><pub>Elsevier B.V</pub><doi>10.1016/j.snb.2005.06.017</doi><tpages>5</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0925-4005
ispartof Sensors and actuators. B, Chemical, 2006-03, Vol.114 (1), p.433-437
issn 0925-4005
1873-3077
language eng
recordid cdi_proquest_miscellaneous_28787853
source Elsevier ScienceDirect Journals Complete
subjects Aptamer
Electrochemical impedance spectroscopy
Label-free protein detection
Thrombin
title Label-free protein recognition using an aptamer-based impedance measurement assay
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-21T15%3A33%3A12IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Label-free%20protein%20recognition%20using%20an%20aptamer-based%20impedance%20measurement%20assay&rft.jtitle=Sensors%20and%20actuators.%20B,%20Chemical&rft.au=Cai,%20Hong&rft.date=2006-03-30&rft.volume=114&rft.issue=1&rft.spage=433&rft.epage=437&rft.pages=433-437&rft.issn=0925-4005&rft.eissn=1873-3077&rft_id=info:doi/10.1016/j.snb.2005.06.017&rft_dat=%3Cproquest_cross%3E28787853%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=19427115&rft_id=info:pmid/&rft_els_id=S0925400505005460&rfr_iscdi=true