Proteomic and phosphoproteomic characterisation of primary mouse embryonic fibroblasts
Fibroblasts are the most common cell type in stroma and function in the support and repair of most tissues. Mouse embryonic fibroblasts (MEFs) are amenable to isolation and rapid growth in culture. MEFs are therefore widely used as a standard model for functional characterisation of gene knockouts,...
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| Veröffentlicht in: | Proteomics (Weinheim) 2024-04, Vol.24 (7), p.e2300267-n/a |
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| creator | Chen, Yanfang Roselli, Severine Panicker, Nikita Brzozowski, Joshua S. Skerrett‐Byrne, David A. Murray, Heather C. Verrills, Nicole M. |
| description | Fibroblasts are the most common cell type in stroma and function in the support and repair of most tissues. Mouse embryonic fibroblasts (MEFs) are amenable to isolation and rapid growth in culture. MEFs are therefore widely used as a standard model for functional characterisation of gene knockouts, and can also be used in co‐cultures, commonly to support embryonic stem cell cultures. To facilitate their use as a research tool, we have performed a comprehensive proteomic and phosphoproteomic characterisation of wild‐type primary MEFs from C57BL/6 mice. EIF2/4 and MTOR signalling pathways were abundant in both the proteome and phosphoproteome, along with extracellular matrix (ECM) and cytoskeleton associated pathways. Consistent with this, kinase enrichment analysis identified activation of P38A, P90RSK, P70S6K, and MTOR. Cell surface markers and matrisome proteins were also annotated. Data are available via ProteomeXchange with identifier PXD043244. This provides a comprehensive catalogue of the wild‐type MEF proteome and phosphoproteome which can be utilised by the field to guide future work. |
| doi_str_mv | 10.1002/pmic.202300267 |
| format | Article |
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Mouse embryonic fibroblasts (MEFs) are amenable to isolation and rapid growth in culture. MEFs are therefore widely used as a standard model for functional characterisation of gene knockouts, and can also be used in co‐cultures, commonly to support embryonic stem cell cultures. To facilitate their use as a research tool, we have performed a comprehensive proteomic and phosphoproteomic characterisation of wild‐type primary MEFs from C57BL/6 mice. EIF2/4 and MTOR signalling pathways were abundant in both the proteome and phosphoproteome, along with extracellular matrix (ECM) and cytoskeleton associated pathways. Consistent with this, kinase enrichment analysis identified activation of P38A, P90RSK, P70S6K, and MTOR. Cell surface markers and matrisome proteins were also annotated. Data are available via ProteomeXchange with identifier PXD043244. This provides a comprehensive catalogue of the wild‐type MEF proteome and phosphoproteome which can be utilised by the field to guide future work.</description><identifier>ISSN: 1615-9853</identifier><identifier>EISSN: 1615-9861</identifier><identifier>DOI: 10.1002/pmic.202300267</identifier><identifier>PMID: 37849217</identifier><language>eng</language><publisher>Germany: Wiley Subscription Services, Inc</publisher><subject>cancer ; Cell culture ; Cell surface ; Cytoskeleton ; ECM ; Embryo cells ; Embryo fibroblasts ; Extracellular matrix ; Fibroblasts ; Kinases ; phosphoproteomics ; Proteomes ; Proteomics ; Signal transduction ; Stem cells ; Stroma ; Surface markers ; TOR protein</subject><ispartof>Proteomics (Weinheim), 2024-04, Vol.24 (7), p.e2300267-n/a</ispartof><rights>2023 The Authors. published by Wiley‐VCH GmbH.</rights><rights>2023 The Authors. PROTEOMICS published by Wiley‐VCH GmbH.</rights><rights>2023. 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| subjects | cancer Cell culture Cell surface Cytoskeleton ECM Embryo cells Embryo fibroblasts Extracellular matrix Fibroblasts Kinases phosphoproteomics Proteomes Proteomics Signal transduction Stem cells Stroma Surface markers TOR protein |
| title | Proteomic and phosphoproteomic characterisation of primary mouse embryonic fibroblasts |
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