Long-term cryopreservation of whole gingival tissue
Stem cells obtained from the body tissue, such as adipose tissue, dental pulp and gingival tissue. Fresh tissue is often used to isolate and culture for regenerative medicine. However, availability of tissue as and when required is one of the measure issue in regenerative medicine. Cryopreservation...
Gespeichert in:
| Veröffentlicht in: | Cell and tissue banking 2024-06, Vol.25 (2), p.551-558 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 558 |
|---|---|
| container_issue | 2 |
| container_start_page | 551 |
| container_title | Cell and tissue banking |
| container_volume | 25 |
| creator | Rakh, Dipika Kuloli, Anita Kharat, Avinash Sanap, Avinash kheur, Supriya Bhonde, Ramesh Gopalakrishnan, D. |
| description | Stem cells obtained from the body tissue, such as adipose tissue, dental pulp and gingival tissue. Fresh tissue is often used to isolate and culture for regenerative medicine. However, availability of tissue as and when required is one of the measure issue in regenerative medicine. Cryopreservation of tissue provides benefit over tissue availability, storage for significant amount of period and helps preserve the original cell structures. The effects of cryopreservation of gingival tissue for mesenchymal stem cell (MSC) are not well documented; however this process is of increasing importance for regenerative therapies. This study examined the effect of cryopreservation on the long term survival the whole gingival biopsy tissue. We studied cell outgrowth, cell morphology, MSC surface-markers and differentiation of mesenchymal stem cells derived from cryopreserved gingiva. In this study, gingival tissue was cryopreserved for 3, 6, 9 months. Cryopreserved tissue has been thawed and cells were isolated by using explant culture method. The fresh and cryopreserved gingival tissue cells were cultured and characterized for surface marker analysis, CFU-f, population doubling time, and osteogenic, chondrogenic and adipogenic differentiation. The fresh and cryopreserved tissue has similar stem cell properties. Results indicate that cryopreservation of the entire gingival tissue does not affect the properties of stem cells. This opens door for gingival tissue banking for future use in periodontology and regenerative medicine. |
| doi_str_mv | 10.1007/s10561-023-10115-y |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2878710410</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3062781073</sourcerecordid><originalsourceid>FETCH-LOGICAL-c326t-462cc72d07a49c3d50b3201f87735bbe12febccc9334c5c5bd916b9dbef76cb03</originalsourceid><addsrcrecordid>eNp9kE1PwzAMhiMEYmPwBzigSly4BOy4bdojmviSJnGBc9Sk6ejUNSNph_bv6dgAiQMnW_Lj19bD2DnCNQLIm4CQpMhBEEdATPjmgI0xkcTTDOPDoacs5zkRjdhJCAsAAVLQMRuRzBLENBszmrl2zjvrl5HxG7fyNli_LrratZGroo8319hoXrfzel00UVeH0NtTdlQVTbBn-zphr_d3L9NHPnt-eJrezrghkXY8ToUxUpQgizg3VCagSQBWmZSUaG1RVFYbY4YHY5OYRJc5pjovta1kajTQhF3tclfevfc2dGpZB2Obpmit64MSmcwkQoxb9PIPunC9b4fvFEEqZIYgaaDEjjLeheBtpVa-XhZ-oxDUVqnaKVWDUvWlVG2GpYt9dK-XtvxZ-XY4ALQDwjBq59b_3v4n9hO7CIFD</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3062781073</pqid></control><display><type>article</type><title>Long-term cryopreservation of whole gingival tissue</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Rakh, Dipika ; Kuloli, Anita ; Kharat, Avinash ; Sanap, Avinash ; kheur, Supriya ; Bhonde, Ramesh ; Gopalakrishnan, D.</creator><creatorcontrib>Rakh, Dipika ; Kuloli, Anita ; Kharat, Avinash ; Sanap, Avinash ; kheur, Supriya ; Bhonde, Ramesh ; Gopalakrishnan, D.</creatorcontrib><description>Stem cells obtained from the body tissue, such as adipose tissue, dental pulp and gingival tissue. Fresh tissue is often used to isolate and culture for regenerative medicine. However, availability of tissue as and when required is one of the measure issue in regenerative medicine. Cryopreservation of tissue provides benefit over tissue availability, storage for significant amount of period and helps preserve the original cell structures. The effects of cryopreservation of gingival tissue for mesenchymal stem cell (MSC) are not well documented; however this process is of increasing importance for regenerative therapies. This study examined the effect of cryopreservation on the long term survival the whole gingival biopsy tissue. We studied cell outgrowth, cell morphology, MSC surface-markers and differentiation of mesenchymal stem cells derived from cryopreserved gingiva. In this study, gingival tissue was cryopreserved for 3, 6, 9 months. Cryopreserved tissue has been thawed and cells were isolated by using explant culture method. The fresh and cryopreserved gingival tissue cells were cultured and characterized for surface marker analysis, CFU-f, population doubling time, and osteogenic, chondrogenic and adipogenic differentiation. The fresh and cryopreserved tissue has similar stem cell properties. Results indicate that cryopreservation of the entire gingival tissue does not affect the properties of stem cells. This opens door for gingival tissue banking for future use in periodontology and regenerative medicine.</description><identifier>ISSN: 1389-9333</identifier><identifier>EISSN: 1573-6814</identifier><identifier>DOI: 10.1007/s10561-023-10115-y</identifier><identifier>PMID: 37851168</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Adipose tissue ; Adult ; Biomedical and Life Sciences ; Biomedicine ; Biopsy ; Cell Biology ; Cell culture ; Cell Differentiation ; Cell Proliferation ; Cells, Cultured ; Cryopreservation ; Cryopreservation - methods ; Cytology ; Dental pulp ; Full Length Paper ; Gingiva ; Gingiva - cytology ; Humans ; Life Sciences ; Mesenchymal stem cells ; Mesenchymal Stem Cells - cytology ; Osteogenesis ; Regenerative medicine ; Stem cells ; Surface markers ; Time Factors ; Transplant Surgery</subject><ispartof>Cell and tissue banking, 2024-06, Vol.25 (2), p.551-558</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2023. The Author(s), under exclusive licence to Springer Nature B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c326t-462cc72d07a49c3d50b3201f87735bbe12febccc9334c5c5bd916b9dbef76cb03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10561-023-10115-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10561-023-10115-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51297</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37851168$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rakh, Dipika</creatorcontrib><creatorcontrib>Kuloli, Anita</creatorcontrib><creatorcontrib>Kharat, Avinash</creatorcontrib><creatorcontrib>Sanap, Avinash</creatorcontrib><creatorcontrib>kheur, Supriya</creatorcontrib><creatorcontrib>Bhonde, Ramesh</creatorcontrib><creatorcontrib>Gopalakrishnan, D.</creatorcontrib><title>Long-term cryopreservation of whole gingival tissue</title><title>Cell and tissue banking</title><addtitle>Cell Tissue Bank</addtitle><addtitle>Cell Tissue Bank</addtitle><description>Stem cells obtained from the body tissue, such as adipose tissue, dental pulp and gingival tissue. Fresh tissue is often used to isolate and culture for regenerative medicine. However, availability of tissue as and when required is one of the measure issue in regenerative medicine. Cryopreservation of tissue provides benefit over tissue availability, storage for significant amount of period and helps preserve the original cell structures. The effects of cryopreservation of gingival tissue for mesenchymal stem cell (MSC) are not well documented; however this process is of increasing importance for regenerative therapies. This study examined the effect of cryopreservation on the long term survival the whole gingival biopsy tissue. We studied cell outgrowth, cell morphology, MSC surface-markers and differentiation of mesenchymal stem cells derived from cryopreserved gingiva. In this study, gingival tissue was cryopreserved for 3, 6, 9 months. Cryopreserved tissue has been thawed and cells were isolated by using explant culture method. The fresh and cryopreserved gingival tissue cells were cultured and characterized for surface marker analysis, CFU-f, population doubling time, and osteogenic, chondrogenic and adipogenic differentiation. The fresh and cryopreserved tissue has similar stem cell properties. Results indicate that cryopreservation of the entire gingival tissue does not affect the properties of stem cells. This opens door for gingival tissue banking for future use in periodontology and regenerative medicine.</description><subject>Adipose tissue</subject><subject>Adult</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Biopsy</subject><subject>Cell Biology</subject><subject>Cell culture</subject><subject>Cell Differentiation</subject><subject>Cell Proliferation</subject><subject>Cells, Cultured</subject><subject>Cryopreservation</subject><subject>Cryopreservation - methods</subject><subject>Cytology</subject><subject>Dental pulp</subject><subject>Full Length Paper</subject><subject>Gingiva</subject><subject>Gingiva - cytology</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>Mesenchymal stem cells</subject><subject>Mesenchymal Stem Cells - cytology</subject><subject>Osteogenesis</subject><subject>Regenerative medicine</subject><subject>Stem cells</subject><subject>Surface markers</subject><subject>Time Factors</subject><subject>Transplant Surgery</subject><issn>1389-9333</issn><issn>1573-6814</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1PwzAMhiMEYmPwBzigSly4BOy4bdojmviSJnGBc9Sk6ejUNSNph_bv6dgAiQMnW_Lj19bD2DnCNQLIm4CQpMhBEEdATPjmgI0xkcTTDOPDoacs5zkRjdhJCAsAAVLQMRuRzBLENBszmrl2zjvrl5HxG7fyNli_LrratZGroo8319hoXrfzel00UVeH0NtTdlQVTbBn-zphr_d3L9NHPnt-eJrezrghkXY8ToUxUpQgizg3VCagSQBWmZSUaG1RVFYbY4YHY5OYRJc5pjovta1kajTQhF3tclfevfc2dGpZB2Obpmit64MSmcwkQoxb9PIPunC9b4fvFEEqZIYgaaDEjjLeheBtpVa-XhZ-oxDUVqnaKVWDUvWlVG2GpYt9dK-XtvxZ-XY4ALQDwjBq59b_3v4n9hO7CIFD</recordid><startdate>20240601</startdate><enddate>20240601</enddate><creator>Rakh, Dipika</creator><creator>Kuloli, Anita</creator><creator>Kharat, Avinash</creator><creator>Sanap, Avinash</creator><creator>kheur, Supriya</creator><creator>Bhonde, Ramesh</creator><creator>Gopalakrishnan, D.</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>NAPCQ</scope><scope>7X8</scope></search><sort><creationdate>20240601</creationdate><title>Long-term cryopreservation of whole gingival tissue</title><author>Rakh, Dipika ; Kuloli, Anita ; Kharat, Avinash ; Sanap, Avinash ; kheur, Supriya ; Bhonde, Ramesh ; Gopalakrishnan, D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c326t-462cc72d07a49c3d50b3201f87735bbe12febccc9334c5c5bd916b9dbef76cb03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Adipose tissue</topic><topic>Adult</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Biopsy</topic><topic>Cell Biology</topic><topic>Cell culture</topic><topic>Cell Differentiation</topic><topic>Cell Proliferation</topic><topic>Cells, Cultured</topic><topic>Cryopreservation</topic><topic>Cryopreservation - methods</topic><topic>Cytology</topic><topic>Dental pulp</topic><topic>Full Length Paper</topic><topic>Gingiva</topic><topic>Gingiva - cytology</topic><topic>Humans</topic><topic>Life Sciences</topic><topic>Mesenchymal stem cells</topic><topic>Mesenchymal Stem Cells - cytology</topic><topic>Osteogenesis</topic><topic>Regenerative medicine</topic><topic>Stem cells</topic><topic>Surface markers</topic><topic>Time Factors</topic><topic>Transplant Surgery</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rakh, Dipika</creatorcontrib><creatorcontrib>Kuloli, Anita</creatorcontrib><creatorcontrib>Kharat, Avinash</creatorcontrib><creatorcontrib>Sanap, Avinash</creatorcontrib><creatorcontrib>kheur, Supriya</creatorcontrib><creatorcontrib>Bhonde, Ramesh</creatorcontrib><creatorcontrib>Gopalakrishnan, D.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><jtitle>Cell and tissue banking</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rakh, Dipika</au><au>Kuloli, Anita</au><au>Kharat, Avinash</au><au>Sanap, Avinash</au><au>kheur, Supriya</au><au>Bhonde, Ramesh</au><au>Gopalakrishnan, D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Long-term cryopreservation of whole gingival tissue</atitle><jtitle>Cell and tissue banking</jtitle><stitle>Cell Tissue Bank</stitle><addtitle>Cell Tissue Bank</addtitle><date>2024-06-01</date><risdate>2024</risdate><volume>25</volume><issue>2</issue><spage>551</spage><epage>558</epage><pages>551-558</pages><issn>1389-9333</issn><eissn>1573-6814</eissn><abstract>Stem cells obtained from the body tissue, such as adipose tissue, dental pulp and gingival tissue. Fresh tissue is often used to isolate and culture for regenerative medicine. However, availability of tissue as and when required is one of the measure issue in regenerative medicine. Cryopreservation of tissue provides benefit over tissue availability, storage for significant amount of period and helps preserve the original cell structures. The effects of cryopreservation of gingival tissue for mesenchymal stem cell (MSC) are not well documented; however this process is of increasing importance for regenerative therapies. This study examined the effect of cryopreservation on the long term survival the whole gingival biopsy tissue. We studied cell outgrowth, cell morphology, MSC surface-markers and differentiation of mesenchymal stem cells derived from cryopreserved gingiva. In this study, gingival tissue was cryopreserved for 3, 6, 9 months. Cryopreserved tissue has been thawed and cells were isolated by using explant culture method. The fresh and cryopreserved gingival tissue cells were cultured and characterized for surface marker analysis, CFU-f, population doubling time, and osteogenic, chondrogenic and adipogenic differentiation. The fresh and cryopreserved tissue has similar stem cell properties. Results indicate that cryopreservation of the entire gingival tissue does not affect the properties of stem cells. This opens door for gingival tissue banking for future use in periodontology and regenerative medicine.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>37851168</pmid><doi>10.1007/s10561-023-10115-y</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1389-9333 |
| ispartof | Cell and tissue banking, 2024-06, Vol.25 (2), p.551-558 |
| issn | 1389-9333 1573-6814 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2878710410 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Adipose tissue Adult Biomedical and Life Sciences Biomedicine Biopsy Cell Biology Cell culture Cell Differentiation Cell Proliferation Cells, Cultured Cryopreservation Cryopreservation - methods Cytology Dental pulp Full Length Paper Gingiva Gingiva - cytology Humans Life Sciences Mesenchymal stem cells Mesenchymal Stem Cells - cytology Osteogenesis Regenerative medicine Stem cells Surface markers Time Factors Transplant Surgery |
| title | Long-term cryopreservation of whole gingival tissue |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-27T11%3A34%3A30IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Long-term%20cryopreservation%20of%20whole%20gingival%20tissue&rft.jtitle=Cell%20and%20tissue%20banking&rft.au=Rakh,%20Dipika&rft.date=2024-06-01&rft.volume=25&rft.issue=2&rft.spage=551&rft.epage=558&rft.pages=551-558&rft.issn=1389-9333&rft.eissn=1573-6814&rft_id=info:doi/10.1007/s10561-023-10115-y&rft_dat=%3Cproquest_cross%3E3062781073%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3062781073&rft_id=info:pmid/37851168&rfr_iscdi=true |