Detection and quantification of UV-transparent keto-androgens by dinitrophenylhydrazine derivatization for discontinuous kinetic assays

Kinetic assays with recombinant enzymes are critical to determine the steady state kinetic parameters for androgen conversion to understand their role in androgen biosynthesis and metabolism. Detection and quantification of 5α-reduced androgens remain difficult to assay because they are UV-transpare...

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Veröffentlicht in:	Methods in enzymology 2023, Vol.689, p.377
1. Verfasser:	Paulukinas, Ryan D
Format:	Artikel
Sprache:	eng
Schlagworte:	Androgens - chemistry Androgens - metabolism Cell Line, Tumor Dihydrotestosterone - metabolism Hydrazines
Online-Zugang:	Volltext
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