Neuroprotective effect of chitosan oligosaccharide on alcohol-induced hippocampal injury using proteomic analysis
Alcoholism is a serious public health problem, and the abuse of drinking seriously damages the health of people. Chitosan oligosaccharides (COSs) are small-molecule oligosaccharides with amino groups that have many unique properties. The neuroprotective effect of COS on alcohol-induced hippocampal i...
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| Veröffentlicht in: | Journal of food science 2023-11, Vol.88 (11), p.4718-4730 |
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| creator | Wang, Bin Zhao, Yuke Qu, Yufei Lu, Jingyu Yan, Hua Gu, Juan Jiang, Qiqing Xu, Yanshun Xia, Wenshui |
| description | Alcoholism is a serious public health problem, and the abuse of drinking seriously damages the health of people. Chitosan oligosaccharides (COSs) are small-molecule oligosaccharides with amino groups that have many unique properties. The neuroprotective effect of COS on alcohol-induced hippocampal injury in Sprague-Dawley (SD) rats was investigated. The discrimination ratio of the COS group in the Y-maze experiment was 59.3% higher than that of the ETOH group. Meanwhile, the discrimination index was less than 0 in the ETOH group but greater than 0 in the COS group during the object recognition test. The cells in the COS group were more tightly arranged than those in the ETOH group. Proteomics was used to identify differentially expressed proteins in the hippocampus. There were 27 differentially expressed proteins in the COS and ETOH group for further bioinformatic analysis. There are three enriched pathway categories, namely, primary immunodeficiency, hedgehog signaling, and sulfur relay system. Next, sonic hedgehog signaling pathway-related proteins were verified through western blotting. The protein expression level of β-arrestin-2 in the COS group was 2.85 times higher than that in the ETOH group. This work may contribute to understanding the underlying mechanism of the neuroprotective effect of COS against alcohol-induced hippocampal injury in SD rats. |
| doi_str_mv | 10.1111/1750-3841.16778 |
| format | Article |
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Chitosan oligosaccharides (COSs) are small-molecule oligosaccharides with amino groups that have many unique properties. The neuroprotective effect of COS on alcohol-induced hippocampal injury in Sprague-Dawley (SD) rats was investigated. The discrimination ratio of the COS group in the Y-maze experiment was 59.3% higher than that of the ETOH group. Meanwhile, the discrimination index was less than 0 in the ETOH group but greater than 0 in the COS group during the object recognition test. The cells in the COS group were more tightly arranged than those in the ETOH group. Proteomics was used to identify differentially expressed proteins in the hippocampus. There were 27 differentially expressed proteins in the COS and ETOH group for further bioinformatic analysis. There are three enriched pathway categories, namely, primary immunodeficiency, hedgehog signaling, and sulfur relay system. Next, sonic hedgehog signaling pathway-related proteins were verified through western blotting. The protein expression level of β-arrestin-2 in the COS group was 2.85 times higher than that in the ETOH group. This work may contribute to understanding the underlying mechanism of the neuroprotective effect of COS against alcohol-induced hippocampal injury in SD rats.</description><identifier>ISSN: 0022-1147</identifier><identifier>EISSN: 1750-3841</identifier><identifier>DOI: 10.1111/1750-3841.16778</identifier><identifier>PMID: 37799098</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Alcohol ; Amino groups ; Animals ; Arrestin ; Chitosan ; Chitosan - pharmacology ; Drinking behavior ; Ethanol ; Ethanol - toxicity ; Hedgehog protein ; Hedgehog Proteins ; Hippocampus ; Humans ; Immunodeficiency ; Injuries ; Injury analysis ; Neuroprotection ; Neuroprotective Agents - pharmacology ; Object recognition ; Oligosaccharides ; Oligosaccharides - pharmacology ; Pattern recognition ; Primary immunodeficiencies ; Proteins ; Proteomics ; Public health ; Rats ; Rats, Sprague-Dawley ; Relay systems ; Signal transduction ; Sulfur ; Western blotting</subject><ispartof>Journal of food science, 2023-11, Vol.88 (11), p.4718-4730</ispartof><rights>2023 Institute of Food Technologists.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c279t-85a01d8d368c6d61e853806298ca1570e32d2ce5910058d23b094ac4f17de33e3</cites><orcidid>0000-0002-1724-9481</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27907,27908</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37799098$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Bin</creatorcontrib><creatorcontrib>Zhao, Yuke</creatorcontrib><creatorcontrib>Qu, Yufei</creatorcontrib><creatorcontrib>Lu, Jingyu</creatorcontrib><creatorcontrib>Yan, Hua</creatorcontrib><creatorcontrib>Gu, Juan</creatorcontrib><creatorcontrib>Jiang, Qiqing</creatorcontrib><creatorcontrib>Xu, Yanshun</creatorcontrib><creatorcontrib>Xia, Wenshui</creatorcontrib><title>Neuroprotective effect of chitosan oligosaccharide on alcohol-induced hippocampal injury using proteomic analysis</title><title>Journal of food science</title><addtitle>J Food Sci</addtitle><description>Alcoholism is a serious public health problem, and the abuse of drinking seriously damages the health of people. Chitosan oligosaccharides (COSs) are small-molecule oligosaccharides with amino groups that have many unique properties. The neuroprotective effect of COS on alcohol-induced hippocampal injury in Sprague-Dawley (SD) rats was investigated. The discrimination ratio of the COS group in the Y-maze experiment was 59.3% higher than that of the ETOH group. Meanwhile, the discrimination index was less than 0 in the ETOH group but greater than 0 in the COS group during the object recognition test. The cells in the COS group were more tightly arranged than those in the ETOH group. Proteomics was used to identify differentially expressed proteins in the hippocampus. There were 27 differentially expressed proteins in the COS and ETOH group for further bioinformatic analysis. There are three enriched pathway categories, namely, primary immunodeficiency, hedgehog signaling, and sulfur relay system. Next, sonic hedgehog signaling pathway-related proteins were verified through western blotting. The protein expression level of β-arrestin-2 in the COS group was 2.85 times higher than that in the ETOH group. This work may contribute to understanding the underlying mechanism of the neuroprotective effect of COS against alcohol-induced hippocampal injury in SD rats.</description><subject>Alcohol</subject><subject>Amino groups</subject><subject>Animals</subject><subject>Arrestin</subject><subject>Chitosan</subject><subject>Chitosan - pharmacology</subject><subject>Drinking behavior</subject><subject>Ethanol</subject><subject>Ethanol - toxicity</subject><subject>Hedgehog protein</subject><subject>Hedgehog Proteins</subject><subject>Hippocampus</subject><subject>Humans</subject><subject>Immunodeficiency</subject><subject>Injuries</subject><subject>Injury analysis</subject><subject>Neuroprotection</subject><subject>Neuroprotective Agents - pharmacology</subject><subject>Object recognition</subject><subject>Oligosaccharides</subject><subject>Oligosaccharides - pharmacology</subject><subject>Pattern recognition</subject><subject>Primary immunodeficiencies</subject><subject>Proteins</subject><subject>Proteomics</subject><subject>Public health</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Relay systems</subject><subject>Signal transduction</subject><subject>Sulfur</subject><subject>Western blotting</subject><issn>0022-1147</issn><issn>1750-3841</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkctLxDAQxoMouj7O3iTgxUs1jzaPo4gvEL3oucRk6mZpm5q0wv73pu7qwTnMTIZfPoZvEDql5JLmuKKyIgVXJb2kQkq1gxZ_k120IISxgtJSHqDDlFZkfnOxjw64lFoTrRbo8xmmGIYYRrCj_wIMTZM7HBpsl34MyfQ4tP4jN9YuTfQOcOixaW1YhrbwvZssOLz0wxCs6QbTYt-vprjGU_L9B_5RDp232PSmXSefjtFeY9oEJ9t6hN7ubl9vHoqnl_vHm-unwjKpx0JVhlCnHBfKCicoqIorIphW1tBKEuDMMQuVpoRUyjH-TnRpbNlQ6YBz4EfoYqObV_icII1155OFtjU9hCnVTMmSCZJzRs__oaswxbzvTGkiKBFSZ-pqQ9kYUorQ1EP0nYnrmpJ6vkY9e1_P3tc_18g_zra603sH7o__tZ9_A0yqhcE</recordid><startdate>202311</startdate><enddate>202311</enddate><creator>Wang, Bin</creator><creator>Zhao, Yuke</creator><creator>Qu, Yufei</creator><creator>Lu, Jingyu</creator><creator>Yan, Hua</creator><creator>Gu, Juan</creator><creator>Jiang, Qiqing</creator><creator>Xu, Yanshun</creator><creator>Xia, Wenshui</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QR</scope><scope>7ST</scope><scope>7T7</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-1724-9481</orcidid></search><sort><creationdate>202311</creationdate><title>Neuroprotective effect of chitosan oligosaccharide on alcohol-induced hippocampal injury using proteomic analysis</title><author>Wang, Bin ; Zhao, Yuke ; Qu, Yufei ; Lu, Jingyu ; Yan, Hua ; Gu, Juan ; Jiang, Qiqing ; Xu, Yanshun ; Xia, Wenshui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c279t-85a01d8d368c6d61e853806298ca1570e32d2ce5910058d23b094ac4f17de33e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Alcohol</topic><topic>Amino groups</topic><topic>Animals</topic><topic>Arrestin</topic><topic>Chitosan</topic><topic>Chitosan - pharmacology</topic><topic>Drinking behavior</topic><topic>Ethanol</topic><topic>Ethanol - toxicity</topic><topic>Hedgehog protein</topic><topic>Hedgehog Proteins</topic><topic>Hippocampus</topic><topic>Humans</topic><topic>Immunodeficiency</topic><topic>Injuries</topic><topic>Injury analysis</topic><topic>Neuroprotection</topic><topic>Neuroprotective Agents - pharmacology</topic><topic>Object recognition</topic><topic>Oligosaccharides</topic><topic>Oligosaccharides - pharmacology</topic><topic>Pattern recognition</topic><topic>Primary immunodeficiencies</topic><topic>Proteins</topic><topic>Proteomics</topic><topic>Public health</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Relay systems</topic><topic>Signal transduction</topic><topic>Sulfur</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Bin</creatorcontrib><creatorcontrib>Zhao, Yuke</creatorcontrib><creatorcontrib>Qu, Yufei</creatorcontrib><creatorcontrib>Lu, Jingyu</creatorcontrib><creatorcontrib>Yan, Hua</creatorcontrib><creatorcontrib>Gu, Juan</creatorcontrib><creatorcontrib>Jiang, Qiqing</creatorcontrib><creatorcontrib>Xu, Yanshun</creatorcontrib><creatorcontrib>Xia, Wenshui</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of food science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Bin</au><au>Zhao, Yuke</au><au>Qu, Yufei</au><au>Lu, Jingyu</au><au>Yan, Hua</au><au>Gu, Juan</au><au>Jiang, Qiqing</au><au>Xu, Yanshun</au><au>Xia, Wenshui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Neuroprotective effect of chitosan oligosaccharide on alcohol-induced hippocampal injury using proteomic analysis</atitle><jtitle>Journal of food science</jtitle><addtitle>J Food Sci</addtitle><date>2023-11</date><risdate>2023</risdate><volume>88</volume><issue>11</issue><spage>4718</spage><epage>4730</epage><pages>4718-4730</pages><issn>0022-1147</issn><eissn>1750-3841</eissn><abstract>Alcoholism is a serious public health problem, and the abuse of drinking seriously damages the health of people. Chitosan oligosaccharides (COSs) are small-molecule oligosaccharides with amino groups that have many unique properties. The neuroprotective effect of COS on alcohol-induced hippocampal injury in Sprague-Dawley (SD) rats was investigated. The discrimination ratio of the COS group in the Y-maze experiment was 59.3% higher than that of the ETOH group. Meanwhile, the discrimination index was less than 0 in the ETOH group but greater than 0 in the COS group during the object recognition test. The cells in the COS group were more tightly arranged than those in the ETOH group. Proteomics was used to identify differentially expressed proteins in the hippocampus. There were 27 differentially expressed proteins in the COS and ETOH group for further bioinformatic analysis. There are three enriched pathway categories, namely, primary immunodeficiency, hedgehog signaling, and sulfur relay system. Next, sonic hedgehog signaling pathway-related proteins were verified through western blotting. The protein expression level of β-arrestin-2 in the COS group was 2.85 times higher than that in the ETOH group. This work may contribute to understanding the underlying mechanism of the neuroprotective effect of COS against alcohol-induced hippocampal injury in SD rats.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>37799098</pmid><doi>10.1111/1750-3841.16778</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-1724-9481</orcidid></addata></record> |
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| subjects | Alcohol Amino groups Animals Arrestin Chitosan Chitosan - pharmacology Drinking behavior Ethanol Ethanol - toxicity Hedgehog protein Hedgehog Proteins Hippocampus Humans Immunodeficiency Injuries Injury analysis Neuroprotection Neuroprotective Agents - pharmacology Object recognition Oligosaccharides Oligosaccharides - pharmacology Pattern recognition Primary immunodeficiencies Proteins Proteomics Public health Rats Rats, Sprague-Dawley Relay systems Signal transduction Sulfur Western blotting |
| title | Neuroprotective effect of chitosan oligosaccharide on alcohol-induced hippocampal injury using proteomic analysis |
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