Novel Ultralow-Potential Electrochemiluminescence Aptasensor for the Highly Sensitive Detection of Zearalenone Using a Resonance Energy Transfer System
An ultralow-potential electrochemiluminescence (ECL) aptasensor has been designed for zearalenone (ZEN) assay based on a resonance energy transfer (RET) system with SnS2 QDs/g-C3N4 as a novel luminophore and CuO/NH2-UiO-66 as a dual-quencher. SnS2 QDs were loaded onto g-C3N4 nanosheets and enhanced...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2023-10, Vol.95 (40), p.15125-15132 |
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| creator | Xiang, Shi Li, Jingxian Wang, Futing Yang, Hongfen Jiang, Yifei Zhang, Penghui Cai, Ren Tan, Weihong |
| description | An ultralow-potential electrochemiluminescence (ECL) aptasensor has been designed for zearalenone (ZEN) assay based on a resonance energy transfer (RET) system with SnS2 QDs/g-C3N4 as a novel luminophore and CuO/NH2-UiO-66 as a dual-quencher. SnS2 QDs were loaded onto g-C3N4 nanosheets and enhanced the ECL luminescence via strong synergistic effects under an ultralow potential. The UV–vis absorption spectrum of CuO/NH2-UiO-66 exhibits considerable overlap with the ECL emission spectrum of SnS2 QDs/g-C3N4, an important consideration for the RET process. In order to stimulate RET, the ZEN aptamer and complementary DNA are introduced for conjugation between the donor and the acceptor. With the binding interaction between ZEN by its aptamer, CuO/NH2-UiO-66 is removed from the electrode surface, resulting in the inhibition of the RET system and an increase in the ECL signal. Under optimal conditions, the as-prepared aptasensor quantified ZEN from 0.5 μg·mL–1 to 0.1 fg·mL–1 with a low limit of detection of 0.085 fg·mL–1, and it exhibited good stability, excellent specificity, high reproducibility, and desirable practicality. The sensing strategy provides a method for mycotoxins assay to monitor food safety. |
| doi_str_mv | 10.1021/acs.analchem.3c03437 |
| format | Article |
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SnS2 QDs were loaded onto g-C3N4 nanosheets and enhanced the ECL luminescence via strong synergistic effects under an ultralow potential. The UV–vis absorption spectrum of CuO/NH2-UiO-66 exhibits considerable overlap with the ECL emission spectrum of SnS2 QDs/g-C3N4, an important consideration for the RET process. In order to stimulate RET, the ZEN aptamer and complementary DNA are introduced for conjugation between the donor and the acceptor. With the binding interaction between ZEN by its aptamer, CuO/NH2-UiO-66 is removed from the electrode surface, resulting in the inhibition of the RET system and an increase in the ECL signal. Under optimal conditions, the as-prepared aptasensor quantified ZEN from 0.5 μg·mL–1 to 0.1 fg·mL–1 with a low limit of detection of 0.085 fg·mL–1, and it exhibited good stability, excellent specificity, high reproducibility, and desirable practicality. The sensing strategy provides a method for mycotoxins assay to monitor food safety.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/acs.analchem.3c03437</identifier><language>eng</language><publisher>Washington: American Chemical Society</publisher><subject>Absorption spectra ; Aptamers ; Carbon nitride ; Conjugation ; Deoxyribonucleic acid ; DNA ; Electrochemiluminescence ; Energy transfer ; Food safety ; Mycotoxins ; Resonance ; Synergistic effect ; Tin disulfide ; Zearalenone</subject><ispartof>Analytical chemistry (Washington), 2023-10, Vol.95 (40), p.15125-15132</ispartof><rights>2023 American Chemical Society</rights><rights>Copyright American Chemical Society Oct 10, 2023</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a353t-5cc278560df8396b92aa0d8e20212d98bf996f93afb2a5258eef7eb88a95ad163</citedby><cites>FETCH-LOGICAL-a353t-5cc278560df8396b92aa0d8e20212d98bf996f93afb2a5258eef7eb88a95ad163</cites><orcidid>0000-0003-2583-7133 ; 0000-0003-1818-4541 ; 0000-0002-8066-1524</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.analchem.3c03437$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.analchem.3c03437$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>315,782,786,2767,27083,27931,27932,56745,56795</link.rule.ids></links><search><creatorcontrib>Xiang, Shi</creatorcontrib><creatorcontrib>Li, Jingxian</creatorcontrib><creatorcontrib>Wang, Futing</creatorcontrib><creatorcontrib>Yang, Hongfen</creatorcontrib><creatorcontrib>Jiang, Yifei</creatorcontrib><creatorcontrib>Zhang, Penghui</creatorcontrib><creatorcontrib>Cai, Ren</creatorcontrib><creatorcontrib>Tan, Weihong</creatorcontrib><title>Novel Ultralow-Potential Electrochemiluminescence Aptasensor for the Highly Sensitive Detection of Zearalenone Using a Resonance Energy Transfer System</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>An ultralow-potential electrochemiluminescence (ECL) aptasensor has been designed for zearalenone (ZEN) assay based on a resonance energy transfer (RET) system with SnS2 QDs/g-C3N4 as a novel luminophore and CuO/NH2-UiO-66 as a dual-quencher. SnS2 QDs were loaded onto g-C3N4 nanosheets and enhanced the ECL luminescence via strong synergistic effects under an ultralow potential. The UV–vis absorption spectrum of CuO/NH2-UiO-66 exhibits considerable overlap with the ECL emission spectrum of SnS2 QDs/g-C3N4, an important consideration for the RET process. In order to stimulate RET, the ZEN aptamer and complementary DNA are introduced for conjugation between the donor and the acceptor. With the binding interaction between ZEN by its aptamer, CuO/NH2-UiO-66 is removed from the electrode surface, resulting in the inhibition of the RET system and an increase in the ECL signal. Under optimal conditions, the as-prepared aptasensor quantified ZEN from 0.5 μg·mL–1 to 0.1 fg·mL–1 with a low limit of detection of 0.085 fg·mL–1, and it exhibited good stability, excellent specificity, high reproducibility, and desirable practicality. 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Li, Jingxian ; Wang, Futing ; Yang, Hongfen ; Jiang, Yifei ; Zhang, Penghui ; Cai, Ren ; Tan, Weihong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a353t-5cc278560df8396b92aa0d8e20212d98bf996f93afb2a5258eef7eb88a95ad163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Absorption spectra</topic><topic>Aptamers</topic><topic>Carbon nitride</topic><topic>Conjugation</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Electrochemiluminescence</topic><topic>Energy transfer</topic><topic>Food safety</topic><topic>Mycotoxins</topic><topic>Resonance</topic><topic>Synergistic effect</topic><topic>Tin disulfide</topic><topic>Zearalenone</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xiang, Shi</creatorcontrib><creatorcontrib>Li, Jingxian</creatorcontrib><creatorcontrib>Wang, Futing</creatorcontrib><creatorcontrib>Yang, Hongfen</creatorcontrib><creatorcontrib>Jiang, Yifei</creatorcontrib><creatorcontrib>Zhang, Penghui</creatorcontrib><creatorcontrib>Cai, Ren</creatorcontrib><creatorcontrib>Tan, Weihong</creatorcontrib><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xiang, Shi</au><au>Li, Jingxian</au><au>Wang, Futing</au><au>Yang, Hongfen</au><au>Jiang, Yifei</au><au>Zhang, Penghui</au><au>Cai, Ren</au><au>Tan, Weihong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Novel Ultralow-Potential Electrochemiluminescence Aptasensor for the Highly Sensitive Detection of Zearalenone Using a Resonance Energy Transfer System</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2023-10-10</date><risdate>2023</risdate><volume>95</volume><issue>40</issue><spage>15125</spage><epage>15132</epage><pages>15125-15132</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><abstract>An ultralow-potential electrochemiluminescence (ECL) aptasensor has been designed for zearalenone (ZEN) assay based on a resonance energy transfer (RET) system with SnS2 QDs/g-C3N4 as a novel luminophore and CuO/NH2-UiO-66 as a dual-quencher. SnS2 QDs were loaded onto g-C3N4 nanosheets and enhanced the ECL luminescence via strong synergistic effects under an ultralow potential. The UV–vis absorption spectrum of CuO/NH2-UiO-66 exhibits considerable overlap with the ECL emission spectrum of SnS2 QDs/g-C3N4, an important consideration for the RET process. In order to stimulate RET, the ZEN aptamer and complementary DNA are introduced for conjugation between the donor and the acceptor. With the binding interaction between ZEN by its aptamer, CuO/NH2-UiO-66 is removed from the electrode surface, resulting in the inhibition of the RET system and an increase in the ECL signal. Under optimal conditions, the as-prepared aptasensor quantified ZEN from 0.5 μg·mL–1 to 0.1 fg·mL–1 with a low limit of detection of 0.085 fg·mL–1, and it exhibited good stability, excellent specificity, high reproducibility, and desirable practicality. The sensing strategy provides a method for mycotoxins assay to monitor food safety.</abstract><cop>Washington</cop><pub>American Chemical Society</pub><doi>10.1021/acs.analchem.3c03437</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0003-2583-7133</orcidid><orcidid>https://orcid.org/0000-0003-1818-4541</orcidid><orcidid>https://orcid.org/0000-0002-8066-1524</orcidid></addata></record> |
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| subjects | Absorption spectra Aptamers Carbon nitride Conjugation Deoxyribonucleic acid DNA Electrochemiluminescence Energy transfer Food safety Mycotoxins Resonance Synergistic effect Tin disulfide Zearalenone |
| title | Novel Ultralow-Potential Electrochemiluminescence Aptasensor for the Highly Sensitive Detection of Zearalenone Using a Resonance Energy Transfer System |
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