Ultra-Fast Mass Spectrometry in Plant Biochemistry: Response of Winter Wheat Proteomics to Pre-Sowing Treatment with Iron Compounds
In recent years, ultrafast liquid chromatography/mass spectrometry methods have been extensively developed for the use in proteome profiling in biochemical studies. These methods are intended for express monitoring of cell response to biotic stimuli and elucidation of correlation of molecular change...
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| Veröffentlicht in: | Biochemistry (Moscow) 2023-09, Vol.88 (9), p.1390-1403 |
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| creator | Kusainova, Tomiris T. Emekeeva, Daria D. Kazakova, Elizaveta M. Gorshkov, Vladimir A. Kjeldsen, Frank Kuskov, Mikhail L. Zhigach, Alexey N. Olkhovskaya, Irina P. Bogoslovskaya, Olga A. Glushchenko, Natalia N. Tarasova, Irina A. |
| description | In recent years, ultrafast liquid chromatography/mass spectrometry methods have been extensively developed for the use in proteome profiling in biochemical studies. These methods are intended for express monitoring of cell response to biotic stimuli and elucidation of correlation of molecular changes with biological processes and phenotypical changes. New technologies, including the use of nanomaterials, are actively introduced to increase agricultural production. However, this requires complex approbation of new fertilizers and investigation of mechanisms underlying the biotic effects on the germination, growth, and development of plants. The aim of this work was to adapt the method of ultrafast chromatography/mass spectrometry for rapid quantitative profiling of molecular changes in 7-day-old wheat seedlings in response to pre-sowing seed treatment with iron compounds. The used method allows to analyze up to 200 samples per day; its practical value lies in the possibility of express proteomic diagnostics of the biotic action of new treatments, including those intended for agricultural needs. Changes in the regulation of photosynthesis, biosynthesis of chlorophyll and porphyrin- and tetrapyrrole-containing compounds, glycolysis (in shoot tissues), and polysaccharide metabolism (in root tissues) were shown after seed treatment with suspensions containing film-forming polymers (PEG 400, Na-CMC, Na
2
-EDTA), iron (II, III) nanoparticles, or iron (II) sulfate. Observations at the protein levels were consistent with the results of morphometry, superoxide dismutase activity assay, and microelement analysis of 3-day-old germinated seeds and shoots and roots of 7-day-old seedlings. A characteristic molecular signature involving proteins participating in the regulation of photosynthesis and glycolytic process was suggested as a potential marker of the biotic effects of seed treatment with iron compounds, which will be confirmed in further studies. |
| doi_str_mv | 10.1134/S0006297923090183 |
| format | Article |
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2
-EDTA), iron (II, III) nanoparticles, or iron (II) sulfate. Observations at the protein levels were consistent with the results of morphometry, superoxide dismutase activity assay, and microelement analysis of 3-day-old germinated seeds and shoots and roots of 7-day-old seedlings. A characteristic molecular signature involving proteins participating in the regulation of photosynthesis and glycolytic process was suggested as a potential marker of the biotic effects of seed treatment with iron compounds, which will be confirmed in further studies.</description><identifier>ISSN: 0006-2979</identifier><identifier>EISSN: 1608-3040</identifier><identifier>DOI: 10.1134/S0006297923090183</identifier><language>eng</language><publisher>Moscow: Pleiades Publishing</publisher><subject>Agricultural production ; Analysis ; Biochemistry ; Biological activity ; Biomedical and Life Sciences ; Biomedicine ; Bioorganic Chemistry ; Biosynthesis ; Chlorophyll ; Chromatography ; Ethylenediaminetetraacetic acids ; Germination ; Glycolysis ; Growth ; Iron ; Iron compounds ; Life Sciences ; Liquid chromatography ; Mass spectrometry ; Mass spectroscopy ; Methods ; Microbiology ; Morphometry ; Nanomaterials ; Nanoparticles ; Nanotechnology ; New technology ; Photosynthesis ; Phytochemistry ; Plant tissues ; Polyethylene glycol ; Polymer films ; Polymers ; Polysaccharides ; Porphyrins ; Proteins ; Proteomes ; Proteomics ; Scientific imaging ; Seed treatments ; Seedlings ; Seeds ; Superoxide dismutase ; Trace elements ; Wheat ; Winter wheat</subject><ispartof>Biochemistry (Moscow), 2023-09, Vol.88 (9), p.1390-1403</ispartof><rights>Pleiades Publishing, Ltd. 2023</rights><rights>COPYRIGHT 2023 Springer</rights><rights>Pleiades Publishing, Ltd. 2023.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c416t-148eaef9dfa8429d8e1cd98c1459a68aa7312fbb0ae0835f4eed556ef7e8d0453</citedby><cites>FETCH-LOGICAL-c416t-148eaef9dfa8429d8e1cd98c1459a68aa7312fbb0ae0835f4eed556ef7e8d0453</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1134/S0006297923090183$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1134/S0006297923090183$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Kusainova, Tomiris T.</creatorcontrib><creatorcontrib>Emekeeva, Daria D.</creatorcontrib><creatorcontrib>Kazakova, Elizaveta M.</creatorcontrib><creatorcontrib>Gorshkov, Vladimir A.</creatorcontrib><creatorcontrib>Kjeldsen, Frank</creatorcontrib><creatorcontrib>Kuskov, Mikhail L.</creatorcontrib><creatorcontrib>Zhigach, Alexey N.</creatorcontrib><creatorcontrib>Olkhovskaya, Irina P.</creatorcontrib><creatorcontrib>Bogoslovskaya, Olga A.</creatorcontrib><creatorcontrib>Glushchenko, Natalia N.</creatorcontrib><creatorcontrib>Tarasova, Irina A.</creatorcontrib><title>Ultra-Fast Mass Spectrometry in Plant Biochemistry: Response of Winter Wheat Proteomics to Pre-Sowing Treatment with Iron Compounds</title><title>Biochemistry (Moscow)</title><addtitle>Biochemistry Moscow</addtitle><description>In recent years, ultrafast liquid chromatography/mass spectrometry methods have been extensively developed for the use in proteome profiling in biochemical studies. These methods are intended for express monitoring of cell response to biotic stimuli and elucidation of correlation of molecular changes with biological processes and phenotypical changes. New technologies, including the use of nanomaterials, are actively introduced to increase agricultural production. However, this requires complex approbation of new fertilizers and investigation of mechanisms underlying the biotic effects on the germination, growth, and development of plants. The aim of this work was to adapt the method of ultrafast chromatography/mass spectrometry for rapid quantitative profiling of molecular changes in 7-day-old wheat seedlings in response to pre-sowing seed treatment with iron compounds. The used method allows to analyze up to 200 samples per day; its practical value lies in the possibility of express proteomic diagnostics of the biotic action of new treatments, including those intended for agricultural needs. Changes in the regulation of photosynthesis, biosynthesis of chlorophyll and porphyrin- and tetrapyrrole-containing compounds, glycolysis (in shoot tissues), and polysaccharide metabolism (in root tissues) were shown after seed treatment with suspensions containing film-forming polymers (PEG 400, Na-CMC, Na
2
-EDTA), iron (II, III) nanoparticles, or iron (II) sulfate. Observations at the protein levels were consistent with the results of morphometry, superoxide dismutase activity assay, and microelement analysis of 3-day-old germinated seeds and shoots and roots of 7-day-old seedlings. A characteristic molecular signature involving proteins participating in the regulation of photosynthesis and glycolytic process was suggested as a potential marker of the biotic effects of seed treatment with iron compounds, which will be confirmed in further studies.</description><subject>Agricultural production</subject><subject>Analysis</subject><subject>Biochemistry</subject><subject>Biological activity</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Bioorganic Chemistry</subject><subject>Biosynthesis</subject><subject>Chlorophyll</subject><subject>Chromatography</subject><subject>Ethylenediaminetetraacetic acids</subject><subject>Germination</subject><subject>Glycolysis</subject><subject>Growth</subject><subject>Iron</subject><subject>Iron compounds</subject><subject>Life Sciences</subject><subject>Liquid chromatography</subject><subject>Mass spectrometry</subject><subject>Mass 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Pre-Sowing Treatment with Iron Compounds</title><author>Kusainova, Tomiris T. ; Emekeeva, Daria D. ; Kazakova, Elizaveta M. ; Gorshkov, Vladimir A. ; Kjeldsen, Frank ; Kuskov, Mikhail L. ; Zhigach, Alexey N. ; Olkhovskaya, Irina P. ; Bogoslovskaya, Olga A. ; Glushchenko, Natalia N. ; Tarasova, Irina A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c416t-148eaef9dfa8429d8e1cd98c1459a68aa7312fbb0ae0835f4eed556ef7e8d0453</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Agricultural production</topic><topic>Analysis</topic><topic>Biochemistry</topic><topic>Biological activity</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Bioorganic Chemistry</topic><topic>Biosynthesis</topic><topic>Chlorophyll</topic><topic>Chromatography</topic><topic>Ethylenediaminetetraacetic 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Moscow</stitle><date>2023-09-01</date><risdate>2023</risdate><volume>88</volume><issue>9</issue><spage>1390</spage><epage>1403</epage><pages>1390-1403</pages><issn>0006-2979</issn><eissn>1608-3040</eissn><abstract>In recent years, ultrafast liquid chromatography/mass spectrometry methods have been extensively developed for the use in proteome profiling in biochemical studies. These methods are intended for express monitoring of cell response to biotic stimuli and elucidation of correlation of molecular changes with biological processes and phenotypical changes. New technologies, including the use of nanomaterials, are actively introduced to increase agricultural production. However, this requires complex approbation of new fertilizers and investigation of mechanisms underlying the biotic effects on the germination, growth, and development of plants. The aim of this work was to adapt the method of ultrafast chromatography/mass spectrometry for rapid quantitative profiling of molecular changes in 7-day-old wheat seedlings in response to pre-sowing seed treatment with iron compounds. The used method allows to analyze up to 200 samples per day; its practical value lies in the possibility of express proteomic diagnostics of the biotic action of new treatments, including those intended for agricultural needs. Changes in the regulation of photosynthesis, biosynthesis of chlorophyll and porphyrin- and tetrapyrrole-containing compounds, glycolysis (in shoot tissues), and polysaccharide metabolism (in root tissues) were shown after seed treatment with suspensions containing film-forming polymers (PEG 400, Na-CMC, Na
2
-EDTA), iron (II, III) nanoparticles, or iron (II) sulfate. Observations at the protein levels were consistent with the results of morphometry, superoxide dismutase activity assay, and microelement analysis of 3-day-old germinated seeds and shoots and roots of 7-day-old seedlings. A characteristic molecular signature involving proteins participating in the regulation of photosynthesis and glycolytic process was suggested as a potential marker of the biotic effects of seed treatment with iron compounds, which will be confirmed in further studies.</abstract><cop>Moscow</cop><pub>Pleiades Publishing</pub><doi>10.1134/S0006297923090183</doi><tpages>14</tpages></addata></record> |
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| ispartof | Biochemistry (Moscow), 2023-09, Vol.88 (9), p.1390-1403 |
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| source | SpringerLink Journals - AutoHoldings |
| subjects | Agricultural production Analysis Biochemistry Biological activity Biomedical and Life Sciences Biomedicine Bioorganic Chemistry Biosynthesis Chlorophyll Chromatography Ethylenediaminetetraacetic acids Germination Glycolysis Growth Iron Iron compounds Life Sciences Liquid chromatography Mass spectrometry Mass spectroscopy Methods Microbiology Morphometry Nanomaterials Nanoparticles Nanotechnology New technology Photosynthesis Phytochemistry Plant tissues Polyethylene glycol Polymer films Polymers Polysaccharides Porphyrins Proteins Proteomes Proteomics Scientific imaging Seed treatments Seedlings Seeds Superoxide dismutase Trace elements Wheat Winter wheat |
| title | Ultra-Fast Mass Spectrometry in Plant Biochemistry: Response of Winter Wheat Proteomics to Pre-Sowing Treatment with Iron Compounds |
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