Development of Reproducible and Scalable Culture Conditions for In Vitro Maintenance of Pig Embryonic Stem Cells Using the Sandoz Inbred Swiss Mouse Thioguanine-Resistant Ouabain-Resistant Cell Line as a Feeder Layer

Feeder cells play a crucial role in maintaining the pluripotency of embryonic stem cells (ESCs) by secreting various extrinsic regulators, such as extracellular matrix (ECM) proteins and growth factors. Although primary mouse embryonic fibroblasts (MEFs) are the most widely used feeder cell type for...

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Veröffentlicht in:Stem cells and development 2023-12, Vol.32 (23-24), p.747-757
Hauptverfasser: Ahn, Yelim, Jeong, Jinsol, Choi, Kwang-Hwan, Lee, Dong-Kyung, Lee, Mingyun, Lee, Na-Young, Kim, Dae-Yong, Lee, Chang-Kyu
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Sprache:eng
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