A seminested recombinase polymerase amplification assay to detect rickettsial pathogens in clinical samples
Treatment at the early stage of onset is vital for the prognosis of rickettsioses. But the absence of specific clinical symptoms complicates the diagnosis of this condition. Herein we established a seminested recombinase polymerase amplification assay (snRPA-nfo) that enables quick detection and dif...
Gespeichert in:
| Veröffentlicht in: | Diagnostic microbiology and infectious disease 2023-12, Vol.107 (4), p.116067-116067, Article 116067 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 116067 |
|---|---|
| container_issue | 4 |
| container_start_page | 116067 |
| container_title | Diagnostic microbiology and infectious disease |
| container_volume | 107 |
| creator | Zhang, Ying Hai, Yan Duan, Biao Long, Hu Xie, Xiaofei Teng, Zhongqiu Yin, Feifei Wang, Mingliu Xiong, Yanwen Shao, Zhujun Guo, Weidong Qin, Aiping |
| description | Treatment at the early stage of onset is vital for the prognosis of rickettsioses. But the absence of specific clinical symptoms complicates the diagnosis of this condition. Herein we established a seminested recombinase polymerase amplification assay (snRPA-nfo) that enables quick detection and differentiation of rickettsial pathogens in clinical samples with high sensitivity and specificity. The conserved 17-kDa protein gene of Rickettsia sibirica and the 47-kDa protein gene of Orientia tsutsugamushi were targeted for the duplex RPA-nfo assay. The snRPA-nfo assay exhibited an increased LOD in spiked blood samples, up to 1000-fold in comparison to standard RPA-nfo, and a better detection rate (83.3%, 5/6) than TaqMan PCR (16.6%, 1/6, Ct ≤ 35) in clinically confirmed patient blood samples. Thus, snRPA-nfo assay represents a promising alternative to TaqMan PCR in the early diagnosis of rickettsioses for point-of-care testing as well as in resource-limited settings. |
| doi_str_mv | 10.1016/j.diagmicrobio.2023.116067 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2869612133</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0732889323001761</els_id><sourcerecordid>2869612133</sourcerecordid><originalsourceid>FETCH-LOGICAL-c352t-13821ef27772734dfc59e86bc787b64b1eac26dd35caaa3c45b04eeac92805bd3</originalsourceid><addsrcrecordid>eNqNkEtr5DAQhEXYhZ3N7n8QOeXiiR62ZecW8oZALslZyFJ70jO25aiVhfn38TB7yDGnLpqqgvoYO5NiLYWsL7brgG4zok-xw7hWQum1lLWozQlbyca0hRBG_GArYbQqmqbVv9hvoq0QUrWlWLHdFScYcQLKEHgCH8cOJ0fA5zjsR0gH6cZ5wB69yxgn7ojcnufIA2TwmSf0O8iZ0A18dvktbmAijhP3A05LaOB0KAD6w372biD4-_-este725frh-Lp-f7x-uqp8LpSuZC6URJ6ZYxRRpeh91ULTd1505iuLjsJzqs6BF1555z2ZdWJEpZnqxpRdUGfsvNj75zi-8eyzI5IHobBTRA_yKqmbmuppNaL9fJoXQASJejtnHB0aW-lsAfCdmu_ErYHwvZIeAnfHMOwjPmHkCx5hMlDwAVktiHid2o-ATMmjnY</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2869612133</pqid></control><display><type>article</type><title>A seminested recombinase polymerase amplification assay to detect rickettsial pathogens in clinical samples</title><source>Elsevier ScienceDirect Journals</source><creator>Zhang, Ying ; Hai, Yan ; Duan, Biao ; Long, Hu ; Xie, Xiaofei ; Teng, Zhongqiu ; Yin, Feifei ; Wang, Mingliu ; Xiong, Yanwen ; Shao, Zhujun ; Guo, Weidong ; Qin, Aiping</creator><creatorcontrib>Zhang, Ying ; Hai, Yan ; Duan, Biao ; Long, Hu ; Xie, Xiaofei ; Teng, Zhongqiu ; Yin, Feifei ; Wang, Mingliu ; Xiong, Yanwen ; Shao, Zhujun ; Guo, Weidong ; Qin, Aiping</creatorcontrib><description>Treatment at the early stage of onset is vital for the prognosis of rickettsioses. But the absence of specific clinical symptoms complicates the diagnosis of this condition. Herein we established a seminested recombinase polymerase amplification assay (snRPA-nfo) that enables quick detection and differentiation of rickettsial pathogens in clinical samples with high sensitivity and specificity. The conserved 17-kDa protein gene of Rickettsia sibirica and the 47-kDa protein gene of Orientia tsutsugamushi were targeted for the duplex RPA-nfo assay. The snRPA-nfo assay exhibited an increased LOD in spiked blood samples, up to 1000-fold in comparison to standard RPA-nfo, and a better detection rate (83.3%, 5/6) than TaqMan PCR (16.6%, 1/6, Ct ≤ 35) in clinically confirmed patient blood samples. Thus, snRPA-nfo assay represents a promising alternative to TaqMan PCR in the early diagnosis of rickettsioses for point-of-care testing as well as in resource-limited settings.</description><identifier>ISSN: 0732-8893</identifier><identifier>EISSN: 1879-0070</identifier><identifier>DOI: 10.1016/j.diagmicrobio.2023.116067</identifier><language>eng</language><publisher>Elsevier Inc</publisher><subject>Orientia tsutsugamushi ; Recombinase polymerase amplification (RPA) ; Rickettsia spp ; Rickettsioses ; Seminested RPA</subject><ispartof>Diagnostic microbiology and infectious disease, 2023-12, Vol.107 (4), p.116067-116067, Article 116067</ispartof><rights>2023 The Authors</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c352t-13821ef27772734dfc59e86bc787b64b1eac26dd35caaa3c45b04eeac92805bd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0732889323001761$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids></links><search><creatorcontrib>Zhang, Ying</creatorcontrib><creatorcontrib>Hai, Yan</creatorcontrib><creatorcontrib>Duan, Biao</creatorcontrib><creatorcontrib>Long, Hu</creatorcontrib><creatorcontrib>Xie, Xiaofei</creatorcontrib><creatorcontrib>Teng, Zhongqiu</creatorcontrib><creatorcontrib>Yin, Feifei</creatorcontrib><creatorcontrib>Wang, Mingliu</creatorcontrib><creatorcontrib>Xiong, Yanwen</creatorcontrib><creatorcontrib>Shao, Zhujun</creatorcontrib><creatorcontrib>Guo, Weidong</creatorcontrib><creatorcontrib>Qin, Aiping</creatorcontrib><title>A seminested recombinase polymerase amplification assay to detect rickettsial pathogens in clinical samples</title><title>Diagnostic microbiology and infectious disease</title><description>Treatment at the early stage of onset is vital for the prognosis of rickettsioses. But the absence of specific clinical symptoms complicates the diagnosis of this condition. Herein we established a seminested recombinase polymerase amplification assay (snRPA-nfo) that enables quick detection and differentiation of rickettsial pathogens in clinical samples with high sensitivity and specificity. The conserved 17-kDa protein gene of Rickettsia sibirica and the 47-kDa protein gene of Orientia tsutsugamushi were targeted for the duplex RPA-nfo assay. The snRPA-nfo assay exhibited an increased LOD in spiked blood samples, up to 1000-fold in comparison to standard RPA-nfo, and a better detection rate (83.3%, 5/6) than TaqMan PCR (16.6%, 1/6, Ct ≤ 35) in clinically confirmed patient blood samples. Thus, snRPA-nfo assay represents a promising alternative to TaqMan PCR in the early diagnosis of rickettsioses for point-of-care testing as well as in resource-limited settings.</description><subject>Orientia tsutsugamushi</subject><subject>Recombinase polymerase amplification (RPA)</subject><subject>Rickettsia spp</subject><subject>Rickettsioses</subject><subject>Seminested RPA</subject><issn>0732-8893</issn><issn>1879-0070</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNqNkEtr5DAQhEXYhZ3N7n8QOeXiiR62ZecW8oZALslZyFJ70jO25aiVhfn38TB7yDGnLpqqgvoYO5NiLYWsL7brgG4zok-xw7hWQum1lLWozQlbyca0hRBG_GArYbQqmqbVv9hvoq0QUrWlWLHdFScYcQLKEHgCH8cOJ0fA5zjsR0gH6cZ5wB69yxgn7ojcnufIA2TwmSf0O8iZ0A18dvktbmAijhP3A05LaOB0KAD6w372biD4-_-este725frh-Lp-f7x-uqp8LpSuZC6URJ6ZYxRRpeh91ULTd1505iuLjsJzqs6BF1555z2ZdWJEpZnqxpRdUGfsvNj75zi-8eyzI5IHobBTRA_yKqmbmuppNaL9fJoXQASJejtnHB0aW-lsAfCdmu_ErYHwvZIeAnfHMOwjPmHkCx5hMlDwAVktiHid2o-ATMmjnY</recordid><startdate>202312</startdate><enddate>202312</enddate><creator>Zhang, Ying</creator><creator>Hai, Yan</creator><creator>Duan, Biao</creator><creator>Long, Hu</creator><creator>Xie, Xiaofei</creator><creator>Teng, Zhongqiu</creator><creator>Yin, Feifei</creator><creator>Wang, Mingliu</creator><creator>Xiong, Yanwen</creator><creator>Shao, Zhujun</creator><creator>Guo, Weidong</creator><creator>Qin, Aiping</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202312</creationdate><title>A seminested recombinase polymerase amplification assay to detect rickettsial pathogens in clinical samples</title><author>Zhang, Ying ; Hai, Yan ; Duan, Biao ; Long, Hu ; Xie, Xiaofei ; Teng, Zhongqiu ; Yin, Feifei ; Wang, Mingliu ; Xiong, Yanwen ; Shao, Zhujun ; Guo, Weidong ; Qin, Aiping</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c352t-13821ef27772734dfc59e86bc787b64b1eac26dd35caaa3c45b04eeac92805bd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Orientia tsutsugamushi</topic><topic>Recombinase polymerase amplification (RPA)</topic><topic>Rickettsia spp</topic><topic>Rickettsioses</topic><topic>Seminested RPA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Ying</creatorcontrib><creatorcontrib>Hai, Yan</creatorcontrib><creatorcontrib>Duan, Biao</creatorcontrib><creatorcontrib>Long, Hu</creatorcontrib><creatorcontrib>Xie, Xiaofei</creatorcontrib><creatorcontrib>Teng, Zhongqiu</creatorcontrib><creatorcontrib>Yin, Feifei</creatorcontrib><creatorcontrib>Wang, Mingliu</creatorcontrib><creatorcontrib>Xiong, Yanwen</creatorcontrib><creatorcontrib>Shao, Zhujun</creatorcontrib><creatorcontrib>Guo, Weidong</creatorcontrib><creatorcontrib>Qin, Aiping</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Diagnostic microbiology and infectious disease</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Ying</au><au>Hai, Yan</au><au>Duan, Biao</au><au>Long, Hu</au><au>Xie, Xiaofei</au><au>Teng, Zhongqiu</au><au>Yin, Feifei</au><au>Wang, Mingliu</au><au>Xiong, Yanwen</au><au>Shao, Zhujun</au><au>Guo, Weidong</au><au>Qin, Aiping</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A seminested recombinase polymerase amplification assay to detect rickettsial pathogens in clinical samples</atitle><jtitle>Diagnostic microbiology and infectious disease</jtitle><date>2023-12</date><risdate>2023</risdate><volume>107</volume><issue>4</issue><spage>116067</spage><epage>116067</epage><pages>116067-116067</pages><artnum>116067</artnum><issn>0732-8893</issn><eissn>1879-0070</eissn><abstract>Treatment at the early stage of onset is vital for the prognosis of rickettsioses. But the absence of specific clinical symptoms complicates the diagnosis of this condition. Herein we established a seminested recombinase polymerase amplification assay (snRPA-nfo) that enables quick detection and differentiation of rickettsial pathogens in clinical samples with high sensitivity and specificity. The conserved 17-kDa protein gene of Rickettsia sibirica and the 47-kDa protein gene of Orientia tsutsugamushi were targeted for the duplex RPA-nfo assay. The snRPA-nfo assay exhibited an increased LOD in spiked blood samples, up to 1000-fold in comparison to standard RPA-nfo, and a better detection rate (83.3%, 5/6) than TaqMan PCR (16.6%, 1/6, Ct ≤ 35) in clinically confirmed patient blood samples. Thus, snRPA-nfo assay represents a promising alternative to TaqMan PCR in the early diagnosis of rickettsioses for point-of-care testing as well as in resource-limited settings.</abstract><pub>Elsevier Inc</pub><doi>10.1016/j.diagmicrobio.2023.116067</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0732-8893 |
| ispartof | Diagnostic microbiology and infectious disease, 2023-12, Vol.107 (4), p.116067-116067, Article 116067 |
| issn | 0732-8893 1879-0070 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2869612133 |
| source | Elsevier ScienceDirect Journals |
| subjects | Orientia tsutsugamushi Recombinase polymerase amplification (RPA) Rickettsia spp Rickettsioses Seminested RPA |
| title | A seminested recombinase polymerase amplification assay to detect rickettsial pathogens in clinical samples |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-21T21%3A55%3A01IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20seminested%20recombinase%20polymerase%20amplification%20assay%20to%20detect%20rickettsial%20pathogens%20in%20clinical%20samples&rft.jtitle=Diagnostic%20microbiology%20and%20infectious%20disease&rft.au=Zhang,%20Ying&rft.date=2023-12&rft.volume=107&rft.issue=4&rft.spage=116067&rft.epage=116067&rft.pages=116067-116067&rft.artnum=116067&rft.issn=0732-8893&rft.eissn=1879-0070&rft_id=info:doi/10.1016/j.diagmicrobio.2023.116067&rft_dat=%3Cproquest_cross%3E2869612133%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2869612133&rft_id=info:pmid/&rft_els_id=S0732889323001761&rfr_iscdi=true |