Facilitating New Chromium Reducing Microbes to Enhance Hexavalent Chromium Reduction by In Situ Sonoporation-Mediated Gene Transfer in Soils
Chromium (Cr) is a heavy metal with a high toxicity and pathogenicity. Microbial reduction is an effective strategy to remove Cr(VI) at contaminated sites but suffers from the low populations and activities of Cr-reducing microorganisms in soils. This study proposed an in situ sonoporation-mediated...
Gespeichert in:
| Veröffentlicht in: | Environmental science & technology 2023-10, Vol.57 (40), p.15123-15133 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 15133 |
|---|---|
| container_issue | 40 |
| container_start_page | 15123 |
| container_title | Environmental science & technology |
| container_volume | 57 |
| creator | Zhao, Kaichao Zhang, Wenjing Liang, Zhentian Zhao, Hongyu Chai, Juanfen Yang, Yuesuo Teng, Tingting Zhang, Dayi |
| description | Chromium (Cr) is a heavy metal with a high toxicity and pathogenicity. Microbial reduction is an effective strategy to remove Cr(VI) at contaminated sites but suffers from the low populations and activities of Cr-reducing microorganisms in soils. This study proposed an in situ sonoporation-mediated gene transfer approach, which improved soil Cr(VI) reduction performance by delivering exogenous Cr-transporter chrA genes and Cr-reducing yieF genes into soil microorganisms with the aid of ultrasound. Besides the increasing populations of Cr-resistant bacteria and elevated copy numbers of chrA and yieF genes after sonoporation-mediated gene transfer, three new Cr-reducing strains were isolated, among which Comamonas aquatica was confirmed to obtain Cr-resistant capability. In addition, sonoporation-mediated gene transfer was the main driving force significantly shaping soil microbial communities owing to the predominance of Cr-resistant microbes. This study pioneered and evidenced that in situ soil sonoporation-mediated gene transfer could effectively deliver functional genes into soil indigenous microbes to facilitate microbial functions for enhanced bioremediation, e.g., Cr-reduction in this study, showing its feasibility as a chemically green and sustainable remediation strategy for heavy metal contaminated sites. |
| doi_str_mv | 10.1021/acs.est.3c04655 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2869220492</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2877497009</sourcerecordid><originalsourceid>FETCH-LOGICAL-a371t-952783c1ba25dfb7145865a7d1d2e1303c92fa1d151562528e36ccea7baf73883</originalsourceid><addsrcrecordid>eNqFkc1r3DAQxUVoIds0514FvRSKN_qwLPlYlnxB0kKTQG9mLI8bBa-0leS0-R_6R1dmQw-B0tPAm98bmPcIecfZmjPBT8CmNaa8lpbVjVIHZMWVYJUyir8iK8a4rFrZfDskb1J6YIwJycyK_D4D6yaXITv_nX7Gn3RzH8PWzVv6FYfZLuq1szH0mGgO9NTfg7dIL_AXPMKEPr8wZBc87Z_opac3Ls_0JviwCxEWvbrGwUHGgZ6jR3obwacRI3WFDW5Kb8nrEaaEx8_ziNydnd5uLqqrL-eXm09XFUjNc9UqoY20vAehhrHXvFamUaAHPgjkkknbihH4wBVXjVDCoGysRdA9jFoaI4_Ih_3dXQw_5hJat3XJ4jSBxzCnTnIljZBK1P9FhWlaIVjdioK-f4E-hDn68kihtK5bzVhbqJM9VTJNKeLY7aLbQnzqOOuWIrtSZLe4n4ssjo97x7L4e_Jf9B8mmaE_</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2877497009</pqid></control><display><type>article</type><title>Facilitating New Chromium Reducing Microbes to Enhance Hexavalent Chromium Reduction by In Situ Sonoporation-Mediated Gene Transfer in Soils</title><source>American Chemical Society Journals</source><creator>Zhao, Kaichao ; Zhang, Wenjing ; Liang, Zhentian ; Zhao, Hongyu ; Chai, Juanfen ; Yang, Yuesuo ; Teng, Tingting ; Zhang, Dayi</creator><creatorcontrib>Zhao, Kaichao ; Zhang, Wenjing ; Liang, Zhentian ; Zhao, Hongyu ; Chai, Juanfen ; Yang, Yuesuo ; Teng, Tingting ; Zhang, Dayi</creatorcontrib><description>Chromium (Cr) is a heavy metal with a high toxicity and pathogenicity. Microbial reduction is an effective strategy to remove Cr(VI) at contaminated sites but suffers from the low populations and activities of Cr-reducing microorganisms in soils. This study proposed an in situ sonoporation-mediated gene transfer approach, which improved soil Cr(VI) reduction performance by delivering exogenous Cr-transporter chrA genes and Cr-reducing yieF genes into soil microorganisms with the aid of ultrasound. Besides the increasing populations of Cr-resistant bacteria and elevated copy numbers of chrA and yieF genes after sonoporation-mediated gene transfer, three new Cr-reducing strains were isolated, among which Comamonas aquatica was confirmed to obtain Cr-resistant capability. In addition, sonoporation-mediated gene transfer was the main driving force significantly shaping soil microbial communities owing to the predominance of Cr-resistant microbes. This study pioneered and evidenced that in situ soil sonoporation-mediated gene transfer could effectively deliver functional genes into soil indigenous microbes to facilitate microbial functions for enhanced bioremediation, e.g., Cr-reduction in this study, showing its feasibility as a chemically green and sustainable remediation strategy for heavy metal contaminated sites.</description><identifier>ISSN: 0013-936X</identifier><identifier>ISSN: 1520-5851</identifier><identifier>EISSN: 1520-5851</identifier><identifier>DOI: 10.1021/acs.est.3c04655</identifier><language>eng</language><publisher>Easton: American Chemical Society</publisher><subject>Bioremediation ; Bioremediation and Biotechnology ; Chromium ; Comamonas ; Feasibility studies ; Functionals ; Gene transfer ; Genes ; Heavy metals ; Hexavalent chromium ; Microbial activity ; Microorganisms ; Pathogenicity ; Pathogens ; Populations ; soil ; Soil contamination ; Soil improvement ; Soil microorganisms ; Soils ; Sustainable remediation ; technology ; Toxicity ; ultrasonics</subject><ispartof>Environmental science & technology, 2023-10, Vol.57 (40), p.15123-15133</ispartof><rights>2023 American Chemical Society</rights><rights>Copyright American Chemical Society Oct 10, 2023</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a371t-952783c1ba25dfb7145865a7d1d2e1303c92fa1d151562528e36ccea7baf73883</citedby><cites>FETCH-LOGICAL-a371t-952783c1ba25dfb7145865a7d1d2e1303c92fa1d151562528e36ccea7baf73883</cites><orcidid>0000-0002-4175-5982 ; 0000-0001-6263-5384</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.est.3c04655$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.est.3c04655$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids></links><search><creatorcontrib>Zhao, Kaichao</creatorcontrib><creatorcontrib>Zhang, Wenjing</creatorcontrib><creatorcontrib>Liang, Zhentian</creatorcontrib><creatorcontrib>Zhao, Hongyu</creatorcontrib><creatorcontrib>Chai, Juanfen</creatorcontrib><creatorcontrib>Yang, Yuesuo</creatorcontrib><creatorcontrib>Teng, Tingting</creatorcontrib><creatorcontrib>Zhang, Dayi</creatorcontrib><title>Facilitating New Chromium Reducing Microbes to Enhance Hexavalent Chromium Reduction by In Situ Sonoporation-Mediated Gene Transfer in Soils</title><title>Environmental science & technology</title><addtitle>Environ. Sci. Technol</addtitle><description>Chromium (Cr) is a heavy metal with a high toxicity and pathogenicity. Microbial reduction is an effective strategy to remove Cr(VI) at contaminated sites but suffers from the low populations and activities of Cr-reducing microorganisms in soils. This study proposed an in situ sonoporation-mediated gene transfer approach, which improved soil Cr(VI) reduction performance by delivering exogenous Cr-transporter chrA genes and Cr-reducing yieF genes into soil microorganisms with the aid of ultrasound. Besides the increasing populations of Cr-resistant bacteria and elevated copy numbers of chrA and yieF genes after sonoporation-mediated gene transfer, three new Cr-reducing strains were isolated, among which Comamonas aquatica was confirmed to obtain Cr-resistant capability. In addition, sonoporation-mediated gene transfer was the main driving force significantly shaping soil microbial communities owing to the predominance of Cr-resistant microbes. This study pioneered and evidenced that in situ soil sonoporation-mediated gene transfer could effectively deliver functional genes into soil indigenous microbes to facilitate microbial functions for enhanced bioremediation, e.g., Cr-reduction in this study, showing its feasibility as a chemically green and sustainable remediation strategy for heavy metal contaminated sites.</description><subject>Bioremediation</subject><subject>Bioremediation and Biotechnology</subject><subject>Chromium</subject><subject>Comamonas</subject><subject>Feasibility studies</subject><subject>Functionals</subject><subject>Gene transfer</subject><subject>Genes</subject><subject>Heavy metals</subject><subject>Hexavalent chromium</subject><subject>Microbial activity</subject><subject>Microorganisms</subject><subject>Pathogenicity</subject><subject>Pathogens</subject><subject>Populations</subject><subject>soil</subject><subject>Soil contamination</subject><subject>Soil improvement</subject><subject>Soil microorganisms</subject><subject>Soils</subject><subject>Sustainable remediation</subject><subject>technology</subject><subject>Toxicity</subject><subject>ultrasonics</subject><issn>0013-936X</issn><issn>1520-5851</issn><issn>1520-5851</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNqFkc1r3DAQxUVoIds0514FvRSKN_qwLPlYlnxB0kKTQG9mLI8bBa-0leS0-R_6R1dmQw-B0tPAm98bmPcIecfZmjPBT8CmNaa8lpbVjVIHZMWVYJUyir8iK8a4rFrZfDskb1J6YIwJycyK_D4D6yaXITv_nX7Gn3RzH8PWzVv6FYfZLuq1szH0mGgO9NTfg7dIL_AXPMKEPr8wZBc87Z_opac3Ls_0JviwCxEWvbrGwUHGgZ6jR3obwacRI3WFDW5Kb8nrEaaEx8_ziNydnd5uLqqrL-eXm09XFUjNc9UqoY20vAehhrHXvFamUaAHPgjkkknbihH4wBVXjVDCoGysRdA9jFoaI4_Ih_3dXQw_5hJat3XJ4jSBxzCnTnIljZBK1P9FhWlaIVjdioK-f4E-hDn68kihtK5bzVhbqJM9VTJNKeLY7aLbQnzqOOuWIrtSZLe4n4ssjo97x7L4e_Jf9B8mmaE_</recordid><startdate>20231010</startdate><enddate>20231010</enddate><creator>Zhao, Kaichao</creator><creator>Zhang, Wenjing</creator><creator>Liang, Zhentian</creator><creator>Zhao, Hongyu</creator><creator>Chai, Juanfen</creator><creator>Yang, Yuesuo</creator><creator>Teng, Tingting</creator><creator>Zhang, Dayi</creator><general>American Chemical Society</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7ST</scope><scope>7T7</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>SOI</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0002-4175-5982</orcidid><orcidid>https://orcid.org/0000-0001-6263-5384</orcidid></search><sort><creationdate>20231010</creationdate><title>Facilitating New Chromium Reducing Microbes to Enhance Hexavalent Chromium Reduction by In Situ Sonoporation-Mediated Gene Transfer in Soils</title><author>Zhao, Kaichao ; Zhang, Wenjing ; Liang, Zhentian ; Zhao, Hongyu ; Chai, Juanfen ; Yang, Yuesuo ; Teng, Tingting ; Zhang, Dayi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a371t-952783c1ba25dfb7145865a7d1d2e1303c92fa1d151562528e36ccea7baf73883</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Bioremediation</topic><topic>Bioremediation and Biotechnology</topic><topic>Chromium</topic><topic>Comamonas</topic><topic>Feasibility studies</topic><topic>Functionals</topic><topic>Gene transfer</topic><topic>Genes</topic><topic>Heavy metals</topic><topic>Hexavalent chromium</topic><topic>Microbial activity</topic><topic>Microorganisms</topic><topic>Pathogenicity</topic><topic>Pathogens</topic><topic>Populations</topic><topic>soil</topic><topic>Soil contamination</topic><topic>Soil improvement</topic><topic>Soil microorganisms</topic><topic>Soils</topic><topic>Sustainable remediation</topic><topic>technology</topic><topic>Toxicity</topic><topic>ultrasonics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhao, Kaichao</creatorcontrib><creatorcontrib>Zhang, Wenjing</creatorcontrib><creatorcontrib>Liang, Zhentian</creatorcontrib><creatorcontrib>Zhao, Hongyu</creatorcontrib><creatorcontrib>Chai, Juanfen</creatorcontrib><creatorcontrib>Yang, Yuesuo</creatorcontrib><creatorcontrib>Teng, Tingting</creatorcontrib><creatorcontrib>Zhang, Dayi</creatorcontrib><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Environmental science & technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhao, Kaichao</au><au>Zhang, Wenjing</au><au>Liang, Zhentian</au><au>Zhao, Hongyu</au><au>Chai, Juanfen</au><au>Yang, Yuesuo</au><au>Teng, Tingting</au><au>Zhang, Dayi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Facilitating New Chromium Reducing Microbes to Enhance Hexavalent Chromium Reduction by In Situ Sonoporation-Mediated Gene Transfer in Soils</atitle><jtitle>Environmental science & technology</jtitle><addtitle>Environ. Sci. Technol</addtitle><date>2023-10-10</date><risdate>2023</risdate><volume>57</volume><issue>40</issue><spage>15123</spage><epage>15133</epage><pages>15123-15133</pages><issn>0013-936X</issn><issn>1520-5851</issn><eissn>1520-5851</eissn><abstract>Chromium (Cr) is a heavy metal with a high toxicity and pathogenicity. Microbial reduction is an effective strategy to remove Cr(VI) at contaminated sites but suffers from the low populations and activities of Cr-reducing microorganisms in soils. This study proposed an in situ sonoporation-mediated gene transfer approach, which improved soil Cr(VI) reduction performance by delivering exogenous Cr-transporter chrA genes and Cr-reducing yieF genes into soil microorganisms with the aid of ultrasound. Besides the increasing populations of Cr-resistant bacteria and elevated copy numbers of chrA and yieF genes after sonoporation-mediated gene transfer, three new Cr-reducing strains were isolated, among which Comamonas aquatica was confirmed to obtain Cr-resistant capability. In addition, sonoporation-mediated gene transfer was the main driving force significantly shaping soil microbial communities owing to the predominance of Cr-resistant microbes. This study pioneered and evidenced that in situ soil sonoporation-mediated gene transfer could effectively deliver functional genes into soil indigenous microbes to facilitate microbial functions for enhanced bioremediation, e.g., Cr-reduction in this study, showing its feasibility as a chemically green and sustainable remediation strategy for heavy metal contaminated sites.</abstract><cop>Easton</cop><pub>American Chemical Society</pub><doi>10.1021/acs.est.3c04655</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-4175-5982</orcidid><orcidid>https://orcid.org/0000-0001-6263-5384</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0013-936X |
| ispartof | Environmental science & technology, 2023-10, Vol.57 (40), p.15123-15133 |
| issn | 0013-936X 1520-5851 1520-5851 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2869220492 |
| source | American Chemical Society Journals |
| subjects | Bioremediation Bioremediation and Biotechnology Chromium Comamonas Feasibility studies Functionals Gene transfer Genes Heavy metals Hexavalent chromium Microbial activity Microorganisms Pathogenicity Pathogens Populations soil Soil contamination Soil improvement Soil microorganisms Soils Sustainable remediation technology Toxicity ultrasonics |
| title | Facilitating New Chromium Reducing Microbes to Enhance Hexavalent Chromium Reduction by In Situ Sonoporation-Mediated Gene Transfer in Soils |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-29T18%3A33%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Facilitating%20New%20Chromium%20Reducing%20Microbes%20to%20Enhance%20Hexavalent%20Chromium%20Reduction%20by%20In%20Situ%20Sonoporation-Mediated%20Gene%20Transfer%20in%20Soils&rft.jtitle=Environmental%20science%20&%20technology&rft.au=Zhao,%20Kaichao&rft.date=2023-10-10&rft.volume=57&rft.issue=40&rft.spage=15123&rft.epage=15133&rft.pages=15123-15133&rft.issn=0013-936X&rft.eissn=1520-5851&rft_id=info:doi/10.1021/acs.est.3c04655&rft_dat=%3Cproquest_cross%3E2877497009%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2877497009&rft_id=info:pmid/&rfr_iscdi=true |