Peptoniphilus equinus sp. nov., a novel Gram-stain-positive anaerobic coccus isolated from the faeces of a thoroughbred racehorse
A novel, anaerobic, Gram-stain-positive coccoid strain, CBA3646 T , was isolated from the faeces of a thoroughbred racehorse. Phylogenetic analysis based on 16S rRNA gene sequencing yielded results indicative of CBA3646 T representing a member of the genus Peptoniphilus , with the species most close...
Gespeichert in:
| Veröffentlicht in: | International journal of systematic and evolutionary microbiology 2023-01, Vol.73 (9) |
|---|---|
| Hauptverfasser: | , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 9 |
| container_start_page | |
| container_title | International journal of systematic and evolutionary microbiology |
| container_volume | 73 |
| creator | Jung, Hye Su Yun, Jeong Ui Jung, Mi-Ja Song, Hye Seon Kim, Yeon Bee Kim, Yujin Kim, Joon-Gyu Jeon, Che Ok Roh, Seong Woon Whon, Tae Woong Lee, Se Hee |
| description | A novel, anaerobic, Gram-stain-positive coccoid strain, CBA3646
T
, was isolated from the faeces of a thoroughbred racehorse. Phylogenetic analysis based on 16S rRNA gene sequencing yielded results indicative of CBA3646
T
representing a member of the genus
Peptoniphilus
, with the species most closely related to it being
Peptoniphilus asaccharolyticus
DSM 20463
T
, with a similarity of 94.79 %. DNA–DNA relatedness and average nucleotide identity values between CBA3646
T
and
P. asaccharolyticus
DSM 20463
T
were 21.4 and 67.6 %, respectively. CBA3646
T
has a circular chromosomal genome of 1 709 189 bp (45.5 mol% DNA G+C content), containing 1652 genes in total, 1584 predicted protein-coding genes, 3 complete rRNA loci and 47 tRNA genes. The cells were non-motile diplococci, catalase-positive and oxidase-negative. Growth of CBA3646
T
was observed at 20–40 °C (optimal temperature, 35 °C) and in the presence of 0–4 % (w/v) NaCl (optimum concentration, 1 %). The major fatty acids (>10 %) of CBA3646
T
were C
16 : 0
, C
18 : 1
ω9
c
and C
18 : 1
ω9
c
dimethyl acetal, with its major polar lipids being diphosphatidylglycerol and phosphatidylglycerol. The elucidated phylogenetic, physiological, chemotaxonomic and molecular properties are indicative of strain CBA3646
T
representing a novel species of the genus
Peptoniphilus
, or which the name
Peptoniphilus equinus
sp. nov. is proposed. The type strain is CBA3646
T
(= KACC 22890
T
= JCM 35845
T
). |
| doi_str_mv | 10.1099/ijsem.0.006053 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2869219571</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2869219571</sourcerecordid><originalsourceid>FETCH-LOGICAL-c227t-b1ef92d9677464dbc0fe700f1edd75ab3cf9634a1815cb40e372d2dc4ad042dd3</originalsourceid><addsrcrecordid>eNo9kDFPwzAQRiMEEqWwMntkIOHsOE4zogoKUiUYYI4c-0xcJXFqO5UY-ecEipi-u9O7b3hJck0ho1BVd3YXsM8gAxBQ5CfJgnIh0gJyfvo_M3GeXISwA5gPAIvk6xXH6AY7trabAsH9ZIc5w5iRwR2yWyJ_Ejuy8bJPQ5R2SEcXbLQHJHKQ6F1jFVFOqfnNBtfJiJoY73oSWyRGosJAnJmLYuu8mz7axs-ElwrnPeBlcmZkF_DqL5fJ--PD2_op3b5sntf321QxVsa0oWgqpitRllxw3SgwWAIYilqXhWxyZSqRc0lXtFANB8xLpplWXGrgTOt8mdwce0fv9hOGWPc2KOw6OaCbQs1WomK0Kko6o9kRVd6F4NHUo7e99J81hfrHdf3ruob66Dr_Boridew</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2869219571</pqid></control><display><type>article</type><title>Peptoniphilus equinus sp. nov., a novel Gram-stain-positive anaerobic coccus isolated from the faeces of a thoroughbred racehorse</title><source>Microbiology Society</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Jung, Hye Su ; Yun, Jeong Ui ; Jung, Mi-Ja ; Song, Hye Seon ; Kim, Yeon Bee ; Kim, Yujin ; Kim, Joon-Gyu ; Jeon, Che Ok ; Roh, Seong Woon ; Whon, Tae Woong ; Lee, Se Hee</creator><creatorcontrib>Jung, Hye Su ; Yun, Jeong Ui ; Jung, Mi-Ja ; Song, Hye Seon ; Kim, Yeon Bee ; Kim, Yujin ; Kim, Joon-Gyu ; Jeon, Che Ok ; Roh, Seong Woon ; Whon, Tae Woong ; Lee, Se Hee</creatorcontrib><description>A novel, anaerobic, Gram-stain-positive coccoid strain, CBA3646
T
, was isolated from the faeces of a thoroughbred racehorse. Phylogenetic analysis based on 16S rRNA gene sequencing yielded results indicative of CBA3646
T
representing a member of the genus
Peptoniphilus
, with the species most closely related to it being
Peptoniphilus asaccharolyticus
DSM 20463
T
, with a similarity of 94.79 %. DNA–DNA relatedness and average nucleotide identity values between CBA3646
T
and
P. asaccharolyticus
DSM 20463
T
were 21.4 and 67.6 %, respectively. CBA3646
T
has a circular chromosomal genome of 1 709 189 bp (45.5 mol% DNA G+C content), containing 1652 genes in total, 1584 predicted protein-coding genes, 3 complete rRNA loci and 47 tRNA genes. The cells were non-motile diplococci, catalase-positive and oxidase-negative. Growth of CBA3646
T
was observed at 20–40 °C (optimal temperature, 35 °C) and in the presence of 0–4 % (w/v) NaCl (optimum concentration, 1 %). The major fatty acids (>10 %) of CBA3646
T
were C
16 : 0
, C
18 : 1
ω9
c
and C
18 : 1
ω9
c
dimethyl acetal, with its major polar lipids being diphosphatidylglycerol and phosphatidylglycerol. The elucidated phylogenetic, physiological, chemotaxonomic and molecular properties are indicative of strain CBA3646
T
representing a novel species of the genus
Peptoniphilus
, or which the name
Peptoniphilus equinus
sp. nov. is proposed. The type strain is CBA3646
T
(= KACC 22890
T
= JCM 35845
T
).</description><identifier>ISSN: 1466-5026</identifier><identifier>EISSN: 1466-5034</identifier><identifier>DOI: 10.1099/ijsem.0.006053</identifier><language>eng</language><ispartof>International journal of systematic and evolutionary microbiology, 2023-01, Vol.73 (9)</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c227t-b1ef92d9677464dbc0fe700f1edd75ab3cf9634a1815cb40e372d2dc4ad042dd3</cites><orcidid>0000-0002-2814-1863</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3746,27924,27925</link.rule.ids></links><search><creatorcontrib>Jung, Hye Su</creatorcontrib><creatorcontrib>Yun, Jeong Ui</creatorcontrib><creatorcontrib>Jung, Mi-Ja</creatorcontrib><creatorcontrib>Song, Hye Seon</creatorcontrib><creatorcontrib>Kim, Yeon Bee</creatorcontrib><creatorcontrib>Kim, Yujin</creatorcontrib><creatorcontrib>Kim, Joon-Gyu</creatorcontrib><creatorcontrib>Jeon, Che Ok</creatorcontrib><creatorcontrib>Roh, Seong Woon</creatorcontrib><creatorcontrib>Whon, Tae Woong</creatorcontrib><creatorcontrib>Lee, Se Hee</creatorcontrib><title>Peptoniphilus equinus sp. nov., a novel Gram-stain-positive anaerobic coccus isolated from the faeces of a thoroughbred racehorse</title><title>International journal of systematic and evolutionary microbiology</title><description>A novel, anaerobic, Gram-stain-positive coccoid strain, CBA3646
T
, was isolated from the faeces of a thoroughbred racehorse. Phylogenetic analysis based on 16S rRNA gene sequencing yielded results indicative of CBA3646
T
representing a member of the genus
Peptoniphilus
, with the species most closely related to it being
Peptoniphilus asaccharolyticus
DSM 20463
T
, with a similarity of 94.79 %. DNA–DNA relatedness and average nucleotide identity values between CBA3646
T
and
P. asaccharolyticus
DSM 20463
T
were 21.4 and 67.6 %, respectively. CBA3646
T
has a circular chromosomal genome of 1 709 189 bp (45.5 mol% DNA G+C content), containing 1652 genes in total, 1584 predicted protein-coding genes, 3 complete rRNA loci and 47 tRNA genes. The cells were non-motile diplococci, catalase-positive and oxidase-negative. Growth of CBA3646
T
was observed at 20–40 °C (optimal temperature, 35 °C) and in the presence of 0–4 % (w/v) NaCl (optimum concentration, 1 %). The major fatty acids (>10 %) of CBA3646
T
were C
16 : 0
, C
18 : 1
ω9
c
and C
18 : 1
ω9
c
dimethyl acetal, with its major polar lipids being diphosphatidylglycerol and phosphatidylglycerol. The elucidated phylogenetic, physiological, chemotaxonomic and molecular properties are indicative of strain CBA3646
T
representing a novel species of the genus
Peptoniphilus
, or which the name
Peptoniphilus equinus
sp. nov. is proposed. The type strain is CBA3646
T
(= KACC 22890
T
= JCM 35845
T
).</description><issn>1466-5026</issn><issn>1466-5034</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNo9kDFPwzAQRiMEEqWwMntkIOHsOE4zogoKUiUYYI4c-0xcJXFqO5UY-ecEipi-u9O7b3hJck0ho1BVd3YXsM8gAxBQ5CfJgnIh0gJyfvo_M3GeXISwA5gPAIvk6xXH6AY7trabAsH9ZIc5w5iRwR2yWyJ_Ejuy8bJPQ5R2SEcXbLQHJHKQ6F1jFVFOqfnNBtfJiJoY73oSWyRGosJAnJmLYuu8mz7axs-ElwrnPeBlcmZkF_DqL5fJ--PD2_op3b5sntf321QxVsa0oWgqpitRllxw3SgwWAIYilqXhWxyZSqRc0lXtFANB8xLpplWXGrgTOt8mdwce0fv9hOGWPc2KOw6OaCbQs1WomK0Kko6o9kRVd6F4NHUo7e99J81hfrHdf3ruob66Dr_Boridew</recordid><startdate>20230101</startdate><enddate>20230101</enddate><creator>Jung, Hye Su</creator><creator>Yun, Jeong Ui</creator><creator>Jung, Mi-Ja</creator><creator>Song, Hye Seon</creator><creator>Kim, Yeon Bee</creator><creator>Kim, Yujin</creator><creator>Kim, Joon-Gyu</creator><creator>Jeon, Che Ok</creator><creator>Roh, Seong Woon</creator><creator>Whon, Tae Woong</creator><creator>Lee, Se Hee</creator><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-2814-1863</orcidid></search><sort><creationdate>20230101</creationdate><title>Peptoniphilus equinus sp. nov., a novel Gram-stain-positive anaerobic coccus isolated from the faeces of a thoroughbred racehorse</title><author>Jung, Hye Su ; Yun, Jeong Ui ; Jung, Mi-Ja ; Song, Hye Seon ; Kim, Yeon Bee ; Kim, Yujin ; Kim, Joon-Gyu ; Jeon, Che Ok ; Roh, Seong Woon ; Whon, Tae Woong ; Lee, Se Hee</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c227t-b1ef92d9677464dbc0fe700f1edd75ab3cf9634a1815cb40e372d2dc4ad042dd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jung, Hye Su</creatorcontrib><creatorcontrib>Yun, Jeong Ui</creatorcontrib><creatorcontrib>Jung, Mi-Ja</creatorcontrib><creatorcontrib>Song, Hye Seon</creatorcontrib><creatorcontrib>Kim, Yeon Bee</creatorcontrib><creatorcontrib>Kim, Yujin</creatorcontrib><creatorcontrib>Kim, Joon-Gyu</creatorcontrib><creatorcontrib>Jeon, Che Ok</creatorcontrib><creatorcontrib>Roh, Seong Woon</creatorcontrib><creatorcontrib>Whon, Tae Woong</creatorcontrib><creatorcontrib>Lee, Se Hee</creatorcontrib><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of systematic and evolutionary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jung, Hye Su</au><au>Yun, Jeong Ui</au><au>Jung, Mi-Ja</au><au>Song, Hye Seon</au><au>Kim, Yeon Bee</au><au>Kim, Yujin</au><au>Kim, Joon-Gyu</au><au>Jeon, Che Ok</au><au>Roh, Seong Woon</au><au>Whon, Tae Woong</au><au>Lee, Se Hee</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Peptoniphilus equinus sp. nov., a novel Gram-stain-positive anaerobic coccus isolated from the faeces of a thoroughbred racehorse</atitle><jtitle>International journal of systematic and evolutionary microbiology</jtitle><date>2023-01-01</date><risdate>2023</risdate><volume>73</volume><issue>9</issue><issn>1466-5026</issn><eissn>1466-5034</eissn><abstract>A novel, anaerobic, Gram-stain-positive coccoid strain, CBA3646
T
, was isolated from the faeces of a thoroughbred racehorse. Phylogenetic analysis based on 16S rRNA gene sequencing yielded results indicative of CBA3646
T
representing a member of the genus
Peptoniphilus
, with the species most closely related to it being
Peptoniphilus asaccharolyticus
DSM 20463
T
, with a similarity of 94.79 %. DNA–DNA relatedness and average nucleotide identity values between CBA3646
T
and
P. asaccharolyticus
DSM 20463
T
were 21.4 and 67.6 %, respectively. CBA3646
T
has a circular chromosomal genome of 1 709 189 bp (45.5 mol% DNA G+C content), containing 1652 genes in total, 1584 predicted protein-coding genes, 3 complete rRNA loci and 47 tRNA genes. The cells were non-motile diplococci, catalase-positive and oxidase-negative. Growth of CBA3646
T
was observed at 20–40 °C (optimal temperature, 35 °C) and in the presence of 0–4 % (w/v) NaCl (optimum concentration, 1 %). The major fatty acids (>10 %) of CBA3646
T
were C
16 : 0
, C
18 : 1
ω9
c
and C
18 : 1
ω9
c
dimethyl acetal, with its major polar lipids being diphosphatidylglycerol and phosphatidylglycerol. The elucidated phylogenetic, physiological, chemotaxonomic and molecular properties are indicative of strain CBA3646
T
representing a novel species of the genus
Peptoniphilus
, or which the name
Peptoniphilus equinus
sp. nov. is proposed. The type strain is CBA3646
T
(= KACC 22890
T
= JCM 35845
T
).</abstract><doi>10.1099/ijsem.0.006053</doi><orcidid>https://orcid.org/0000-0002-2814-1863</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1466-5026 |
| ispartof | International journal of systematic and evolutionary microbiology, 2023-01, Vol.73 (9) |
| issn | 1466-5026 1466-5034 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2869219571 |
| source | Microbiology Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| title | Peptoniphilus equinus sp. nov., a novel Gram-stain-positive anaerobic coccus isolated from the faeces of a thoroughbred racehorse |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T20%3A30%3A51IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Peptoniphilus%20equinus%20sp.%20nov.,%20a%20novel%20Gram-stain-positive%20anaerobic%20coccus%20isolated%20from%20the%20faeces%20of%20a%20thoroughbred%20racehorse&rft.jtitle=International%20journal%20of%20systematic%20and%20evolutionary%20microbiology&rft.au=Jung,%20Hye%20Su&rft.date=2023-01-01&rft.volume=73&rft.issue=9&rft.issn=1466-5026&rft.eissn=1466-5034&rft_id=info:doi/10.1099/ijsem.0.006053&rft_dat=%3Cproquest_cross%3E2869219571%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2869219571&rft_id=info:pmid/&rfr_iscdi=true |