Endothelial damage inhibitor preserves the integrity of venous endothelial cells from patients undergoing coronary bypass surgery
Abstract OBJECTIVES Despite the success of coronary artery bypass graft (CABG) surgery using autologous saphenous vein grafts (SVGs), nearly 50% of patients experience vein graft disease within 10 years of surgery. One contributing factor to early vein graft disease is endothelial damage during shor...
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| Veröffentlicht in: | European journal of cardio-thoracic surgery 2023-12, Vol.64 (6) |
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| creator | Nazari-Shafti, Timo Z Thau, Henriette Zacharova, Ema Beez, Christien M Exarchos, Vasileios Neuber, Sebastian Meyborg, Heike Puhl, Kerstin Wittig, Corey Szulcek, Robert Neumann, Konrad Giampietro, Costanza Krüger, Katrin Cesarovic, Nikola Falk, Volkmar Caliskan, Etem Rodriguez Cetina Biefer, Hector Emmert, Maximilian Y |
| description | Abstract
OBJECTIVES
Despite the success of coronary artery bypass graft (CABG) surgery using autologous saphenous vein grafts (SVGs), nearly 50% of patients experience vein graft disease within 10 years of surgery. One contributing factor to early vein graft disease is endothelial damage during short-term storage of SVGs in inappropriate solutions. Our aim was to evaluate the effects of a novel endothelial damage inhibitor (EDI) on SVGs from patients undergoing elective CABG surgery and on venous endothelial cells (VECs) derived from these SVGs.
METHODS
SVGs from 11 patients participating in an ongoing clinical registry (NCT02922088) were included in this study, and incubated with both full electrolyte solution (FES) or EDI for 1 h and then examined histologically. In 8 of 11 patients, VECs were isolated from untreated grafts, incubated with both FES and EDI for 2 h under hypothermic stress conditions and then analysed for activation of an inflammatory phenotype, cell damage and cytotoxicity, as well as endothelial integrity and barrier function.
RESULTS
The EDI was superior to FES in protecting the endothelium in SVGs (74 ± 8% versus 56 ± 8%, P |
| doi_str_mv | 10.1093/ejcts/ezad327 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2868124647</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1093/ejcts/ezad327</oup_id><sourcerecordid>2868124647</sourcerecordid><originalsourceid>FETCH-LOGICAL-c365t-a2dc4bd105817baffe072818b5497b957d577b375c46d2d271735dda20e179063</originalsourceid><addsrcrecordid>eNqFkL1PwzAQxS0EoqUwsiKPLAHb-XAyoqp8SJVYQGKLnPiSukriYDuVwsZ_jksLdGO60-l37949hC4puaEkC29hXTp7Cx9ChowfoSlNeRjwMHo7Pugn6MzaNSEk8dApmoScRySL2RR9Ljqp3QoaJRosRStqwKpbqUI5bXBvwILZgMUe8XMHtVFuxLrCG-j0YDEcrJfQNBZXRre4F05B5yweOgmm1qqrcamN7oQZcTH2wlpsB1ODGc_RSSUaCxf7OkOv94uX-WOwfH54mt8tgzJMYhcIJsuokJTEKeWFqCognKU0LeIo40UWcxlzXoQ8LqNEMsk45WEspWAEKM_84zN0vdPtjX4fwLq8VXZrWXTgP8lZmqSURUnEPRrs0NJoaw1UeW9U663nlOTb1PPv1PN96p6_2ksPRQvyl_6J-e-2Hvp_tL4AL1qRUg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2868124647</pqid></control><display><type>article</type><title>Endothelial damage inhibitor preserves the integrity of venous endothelial cells from patients undergoing coronary bypass surgery</title><source>Oxford University Press Journals All Titles (1996-Current)</source><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><creator>Nazari-Shafti, Timo Z ; Thau, Henriette ; Zacharova, Ema ; Beez, Christien M ; Exarchos, Vasileios ; Neuber, Sebastian ; Meyborg, Heike ; Puhl, Kerstin ; Wittig, Corey ; Szulcek, Robert ; Neumann, Konrad ; Giampietro, Costanza ; Krüger, Katrin ; Cesarovic, Nikola ; Falk, Volkmar ; Caliskan, Etem ; Rodriguez Cetina Biefer, Hector ; Emmert, Maximilian Y</creator><creatorcontrib>Nazari-Shafti, Timo Z ; Thau, Henriette ; Zacharova, Ema ; Beez, Christien M ; Exarchos, Vasileios ; Neuber, Sebastian ; Meyborg, Heike ; Puhl, Kerstin ; Wittig, Corey ; Szulcek, Robert ; Neumann, Konrad ; Giampietro, Costanza ; Krüger, Katrin ; Cesarovic, Nikola ; Falk, Volkmar ; Caliskan, Etem ; Rodriguez Cetina Biefer, Hector ; Emmert, Maximilian Y</creatorcontrib><description>Abstract
OBJECTIVES
Despite the success of coronary artery bypass graft (CABG) surgery using autologous saphenous vein grafts (SVGs), nearly 50% of patients experience vein graft disease within 10 years of surgery. One contributing factor to early vein graft disease is endothelial damage during short-term storage of SVGs in inappropriate solutions. Our aim was to evaluate the effects of a novel endothelial damage inhibitor (EDI) on SVGs from patients undergoing elective CABG surgery and on venous endothelial cells (VECs) derived from these SVGs.
METHODS
SVGs from 11 patients participating in an ongoing clinical registry (NCT02922088) were included in this study, and incubated with both full electrolyte solution (FES) or EDI for 1 h and then examined histologically. In 8 of 11 patients, VECs were isolated from untreated grafts, incubated with both FES and EDI for 2 h under hypothermic stress conditions and then analysed for activation of an inflammatory phenotype, cell damage and cytotoxicity, as well as endothelial integrity and barrier function.
RESULTS
The EDI was superior to FES in protecting the endothelium in SVGs (74 ± 8% versus 56 ± 8%, P < 0.001). Besides confirming that the EDI prevents apoptosis in SVG-derived VECs, we also showed that the EDI temporarily reduces adherens junctions in VECs while protecting focal adhesions compared to FES.
CONCLUSIONS
The EDI protects the connectivity and function of the SVG endothelium. Our data suggest that the EDI can preserve focal adhesions in VECs during short-term storage after graft harvesting. This might explain the superiority of the EDI in maintaining most of the endothelium in venous CABG surgery conduits.
Coronary artery bypass grafting (CABG) with saphenous vein grafts (SVGs) remains the preferred treatment for patients with complex multivessel coronary artery disease, despite the availability of multi and total arterial approaches [1, 2].</description><identifier>ISSN: 1873-734X</identifier><identifier>EISSN: 1873-734X</identifier><identifier>DOI: 10.1093/ejcts/ezad327</identifier><identifier>PMID: 37740952</identifier><language>eng</language><publisher>Germany: Oxford University Press</publisher><subject>Coronary Artery Bypass - adverse effects ; Endothelial Cells ; Endothelium, Vascular ; Humans ; Saphenous Vein - transplantation ; Vascular Diseases ; Vascular Patency - physiology</subject><ispartof>European journal of cardio-thoracic surgery, 2023-12, Vol.64 (6)</ispartof><rights>The Author(s) 2023. Published by Oxford University Press on behalf of the European Association for Cardio-Thoracic Surgery. All rights reserved. 2023</rights><rights>The Author(s) 2023. Published by Oxford University Press on behalf of the European Association for Cardio-Thoracic Surgery. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c365t-a2dc4bd105817baffe072818b5497b957d577b375c46d2d271735dda20e179063</citedby><cites>FETCH-LOGICAL-c365t-a2dc4bd105817baffe072818b5497b957d577b375c46d2d271735dda20e179063</cites><orcidid>0000-0003-1833-8440 ; 0000-0002-9055-6891 ; 0000-0002-9051-7841 ; 0000-0003-1373-636X ; 0000-0002-7911-8620</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37740952$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nazari-Shafti, Timo Z</creatorcontrib><creatorcontrib>Thau, Henriette</creatorcontrib><creatorcontrib>Zacharova, Ema</creatorcontrib><creatorcontrib>Beez, Christien M</creatorcontrib><creatorcontrib>Exarchos, Vasileios</creatorcontrib><creatorcontrib>Neuber, Sebastian</creatorcontrib><creatorcontrib>Meyborg, Heike</creatorcontrib><creatorcontrib>Puhl, Kerstin</creatorcontrib><creatorcontrib>Wittig, Corey</creatorcontrib><creatorcontrib>Szulcek, Robert</creatorcontrib><creatorcontrib>Neumann, Konrad</creatorcontrib><creatorcontrib>Giampietro, Costanza</creatorcontrib><creatorcontrib>Krüger, Katrin</creatorcontrib><creatorcontrib>Cesarovic, Nikola</creatorcontrib><creatorcontrib>Falk, Volkmar</creatorcontrib><creatorcontrib>Caliskan, Etem</creatorcontrib><creatorcontrib>Rodriguez Cetina Biefer, Hector</creatorcontrib><creatorcontrib>Emmert, Maximilian Y</creatorcontrib><title>Endothelial damage inhibitor preserves the integrity of venous endothelial cells from patients undergoing coronary bypass surgery</title><title>European journal of cardio-thoracic surgery</title><addtitle>Eur J Cardiothorac Surg</addtitle><description>Abstract
OBJECTIVES
Despite the success of coronary artery bypass graft (CABG) surgery using autologous saphenous vein grafts (SVGs), nearly 50% of patients experience vein graft disease within 10 years of surgery. One contributing factor to early vein graft disease is endothelial damage during short-term storage of SVGs in inappropriate solutions. Our aim was to evaluate the effects of a novel endothelial damage inhibitor (EDI) on SVGs from patients undergoing elective CABG surgery and on venous endothelial cells (VECs) derived from these SVGs.
METHODS
SVGs from 11 patients participating in an ongoing clinical registry (NCT02922088) were included in this study, and incubated with both full electrolyte solution (FES) or EDI for 1 h and then examined histologically. In 8 of 11 patients, VECs were isolated from untreated grafts, incubated with both FES and EDI for 2 h under hypothermic stress conditions and then analysed for activation of an inflammatory phenotype, cell damage and cytotoxicity, as well as endothelial integrity and barrier function.
RESULTS
The EDI was superior to FES in protecting the endothelium in SVGs (74 ± 8% versus 56 ± 8%, P < 0.001). Besides confirming that the EDI prevents apoptosis in SVG-derived VECs, we also showed that the EDI temporarily reduces adherens junctions in VECs while protecting focal adhesions compared to FES.
CONCLUSIONS
The EDI protects the connectivity and function of the SVG endothelium. Our data suggest that the EDI can preserve focal adhesions in VECs during short-term storage after graft harvesting. This might explain the superiority of the EDI in maintaining most of the endothelium in venous CABG surgery conduits.
Coronary artery bypass grafting (CABG) with saphenous vein grafts (SVGs) remains the preferred treatment for patients with complex multivessel coronary artery disease, despite the availability of multi and total arterial approaches [1, 2].</description><subject>Coronary Artery Bypass - adverse effects</subject><subject>Endothelial Cells</subject><subject>Endothelium, Vascular</subject><subject>Humans</subject><subject>Saphenous Vein - transplantation</subject><subject>Vascular Diseases</subject><subject>Vascular Patency - physiology</subject><issn>1873-734X</issn><issn>1873-734X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkL1PwzAQxS0EoqUwsiKPLAHb-XAyoqp8SJVYQGKLnPiSukriYDuVwsZ_jksLdGO60-l37949hC4puaEkC29hXTp7Cx9ChowfoSlNeRjwMHo7Pugn6MzaNSEk8dApmoScRySL2RR9Ljqp3QoaJRosRStqwKpbqUI5bXBvwILZgMUe8XMHtVFuxLrCG-j0YDEcrJfQNBZXRre4F05B5yweOgmm1qqrcamN7oQZcTH2wlpsB1ODGc_RSSUaCxf7OkOv94uX-WOwfH54mt8tgzJMYhcIJsuokJTEKeWFqCognKU0LeIo40UWcxlzXoQ8LqNEMsk45WEspWAEKM_84zN0vdPtjX4fwLq8VXZrWXTgP8lZmqSURUnEPRrs0NJoaw1UeW9U663nlOTb1PPv1PN96p6_2ksPRQvyl_6J-e-2Hvp_tL4AL1qRUg</recordid><startdate>20231201</startdate><enddate>20231201</enddate><creator>Nazari-Shafti, Timo Z</creator><creator>Thau, Henriette</creator><creator>Zacharova, Ema</creator><creator>Beez, Christien M</creator><creator>Exarchos, Vasileios</creator><creator>Neuber, Sebastian</creator><creator>Meyborg, Heike</creator><creator>Puhl, Kerstin</creator><creator>Wittig, Corey</creator><creator>Szulcek, Robert</creator><creator>Neumann, Konrad</creator><creator>Giampietro, Costanza</creator><creator>Krüger, Katrin</creator><creator>Cesarovic, Nikola</creator><creator>Falk, Volkmar</creator><creator>Caliskan, Etem</creator><creator>Rodriguez Cetina Biefer, Hector</creator><creator>Emmert, Maximilian Y</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-1833-8440</orcidid><orcidid>https://orcid.org/0000-0002-9055-6891</orcidid><orcidid>https://orcid.org/0000-0002-9051-7841</orcidid><orcidid>https://orcid.org/0000-0003-1373-636X</orcidid><orcidid>https://orcid.org/0000-0002-7911-8620</orcidid></search><sort><creationdate>20231201</creationdate><title>Endothelial damage inhibitor preserves the integrity of venous endothelial cells from patients undergoing coronary bypass surgery</title><author>Nazari-Shafti, Timo Z ; Thau, Henriette ; Zacharova, Ema ; Beez, Christien M ; Exarchos, Vasileios ; Neuber, Sebastian ; Meyborg, Heike ; Puhl, Kerstin ; Wittig, Corey ; Szulcek, Robert ; Neumann, Konrad ; Giampietro, Costanza ; Krüger, Katrin ; Cesarovic, Nikola ; Falk, Volkmar ; Caliskan, Etem ; Rodriguez Cetina Biefer, Hector ; Emmert, Maximilian Y</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c365t-a2dc4bd105817baffe072818b5497b957d577b375c46d2d271735dda20e179063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Coronary Artery Bypass - adverse effects</topic><topic>Endothelial Cells</topic><topic>Endothelium, Vascular</topic><topic>Humans</topic><topic>Saphenous Vein - transplantation</topic><topic>Vascular Diseases</topic><topic>Vascular Patency - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nazari-Shafti, Timo Z</creatorcontrib><creatorcontrib>Thau, Henriette</creatorcontrib><creatorcontrib>Zacharova, Ema</creatorcontrib><creatorcontrib>Beez, Christien M</creatorcontrib><creatorcontrib>Exarchos, Vasileios</creatorcontrib><creatorcontrib>Neuber, Sebastian</creatorcontrib><creatorcontrib>Meyborg, Heike</creatorcontrib><creatorcontrib>Puhl, Kerstin</creatorcontrib><creatorcontrib>Wittig, Corey</creatorcontrib><creatorcontrib>Szulcek, Robert</creatorcontrib><creatorcontrib>Neumann, Konrad</creatorcontrib><creatorcontrib>Giampietro, Costanza</creatorcontrib><creatorcontrib>Krüger, Katrin</creatorcontrib><creatorcontrib>Cesarovic, Nikola</creatorcontrib><creatorcontrib>Falk, Volkmar</creatorcontrib><creatorcontrib>Caliskan, Etem</creatorcontrib><creatorcontrib>Rodriguez Cetina Biefer, Hector</creatorcontrib><creatorcontrib>Emmert, Maximilian Y</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of cardio-thoracic surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nazari-Shafti, Timo Z</au><au>Thau, Henriette</au><au>Zacharova, Ema</au><au>Beez, Christien M</au><au>Exarchos, Vasileios</au><au>Neuber, Sebastian</au><au>Meyborg, Heike</au><au>Puhl, Kerstin</au><au>Wittig, Corey</au><au>Szulcek, Robert</au><au>Neumann, Konrad</au><au>Giampietro, Costanza</au><au>Krüger, Katrin</au><au>Cesarovic, Nikola</au><au>Falk, Volkmar</au><au>Caliskan, Etem</au><au>Rodriguez Cetina Biefer, Hector</au><au>Emmert, Maximilian Y</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Endothelial damage inhibitor preserves the integrity of venous endothelial cells from patients undergoing coronary bypass surgery</atitle><jtitle>European journal of cardio-thoracic surgery</jtitle><addtitle>Eur J Cardiothorac Surg</addtitle><date>2023-12-01</date><risdate>2023</risdate><volume>64</volume><issue>6</issue><issn>1873-734X</issn><eissn>1873-734X</eissn><abstract>Abstract
OBJECTIVES
Despite the success of coronary artery bypass graft (CABG) surgery using autologous saphenous vein grafts (SVGs), nearly 50% of patients experience vein graft disease within 10 years of surgery. One contributing factor to early vein graft disease is endothelial damage during short-term storage of SVGs in inappropriate solutions. Our aim was to evaluate the effects of a novel endothelial damage inhibitor (EDI) on SVGs from patients undergoing elective CABG surgery and on venous endothelial cells (VECs) derived from these SVGs.
METHODS
SVGs from 11 patients participating in an ongoing clinical registry (NCT02922088) were included in this study, and incubated with both full electrolyte solution (FES) or EDI for 1 h and then examined histologically. In 8 of 11 patients, VECs were isolated from untreated grafts, incubated with both FES and EDI for 2 h under hypothermic stress conditions and then analysed for activation of an inflammatory phenotype, cell damage and cytotoxicity, as well as endothelial integrity and barrier function.
RESULTS
The EDI was superior to FES in protecting the endothelium in SVGs (74 ± 8% versus 56 ± 8%, P < 0.001). Besides confirming that the EDI prevents apoptosis in SVG-derived VECs, we also showed that the EDI temporarily reduces adherens junctions in VECs while protecting focal adhesions compared to FES.
CONCLUSIONS
The EDI protects the connectivity and function of the SVG endothelium. Our data suggest that the EDI can preserve focal adhesions in VECs during short-term storage after graft harvesting. This might explain the superiority of the EDI in maintaining most of the endothelium in venous CABG surgery conduits.
Coronary artery bypass grafting (CABG) with saphenous vein grafts (SVGs) remains the preferred treatment for patients with complex multivessel coronary artery disease, despite the availability of multi and total arterial approaches [1, 2].</abstract><cop>Germany</cop><pub>Oxford University Press</pub><pmid>37740952</pmid><doi>10.1093/ejcts/ezad327</doi><orcidid>https://orcid.org/0000-0003-1833-8440</orcidid><orcidid>https://orcid.org/0000-0002-9055-6891</orcidid><orcidid>https://orcid.org/0000-0002-9051-7841</orcidid><orcidid>https://orcid.org/0000-0003-1373-636X</orcidid><orcidid>https://orcid.org/0000-0002-7911-8620</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Coronary Artery Bypass - adverse effects Endothelial Cells Endothelium, Vascular Humans Saphenous Vein - transplantation Vascular Diseases Vascular Patency - physiology |
| title | Endothelial damage inhibitor preserves the integrity of venous endothelial cells from patients undergoing coronary bypass surgery |
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