Label-free microfluidic isolation of functional and viable lymphocytes from peripheral blood mononuclear cells

The separation of peripheral blood mononuclear cells (PBMCs) into constituent blood cell types is a vital step to obtain immune cells for autologous cell therapies. The ability to separate PBMCs using label-free microfluidic techniques, based on differences in biomechanical properties, can have a nu...

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Veröffentlicht in:Biomicrofluidics 2023-09, Vol.17 (5), p.054102
Hauptverfasser: Raj, Abhishek, Ramirez, Katily, Young, Katherine M., Stone, Nicholas, Shankles, Peter, Ali, Mehdia Nadeem Rajab, Compton, Anthony Malik, Lam, Wilbur, Alexeev, Alexander, Sulchek, Todd
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container_end_page
container_issue 5
container_start_page 054102
container_title Biomicrofluidics
container_volume 17
creator Raj, Abhishek
Ramirez, Katily
Young, Katherine M.
Stone, Nicholas
Shankles, Peter
Ali, Mehdia Nadeem Rajab
Compton, Anthony Malik
Lam, Wilbur
Alexeev, Alexander
Sulchek, Todd
description The separation of peripheral blood mononuclear cells (PBMCs) into constituent blood cell types is a vital step to obtain immune cells for autologous cell therapies. The ability to separate PBMCs using label-free microfluidic techniques, based on differences in biomechanical properties, can have a number of benefits over other conventional techniques, including lower cost, ease of use, and avoidance of animal-derived labeling antibodies. Here, we report a microfluidic device that uses compressive diagonal ridges to separate PBMCs into highly pure samples of viable and functional lymphocytes. The technique utilizes the differences in the biophysical properties of PBMC sub-populations to direct the lymphocytes and monocytes into separate outlets. The biophysical properties of the monocytes and lymphocytes from healthy donors were first characterized using atomic force microscopy. Lymphocytes were found to be significantly stiffer than monocytes, with a mean cell stiffness of 1495 and 931 Pa, respectively. The differences in biophysical properties resulted in distinct trajectories through the microchannel terminating at different outlets, resulting in a lymphocyte sample with purity and viability both greater than 96% with no effect on the cells’ ability to produce interferon gamma, a cytokine crucial for innate and adaptive immunity.
doi_str_mv 10.1063/5.0161047
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1932-1058
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source AIP Journals Complete; PubMed Central
subjects Antibodies
Biomechanics
Blood
Blood cells
Immune system
Labels
Lymphocytes
Microchannels
Microfluidic devices
Regular
title Label-free microfluidic isolation of functional and viable lymphocytes from peripheral blood mononuclear cells
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