Quantitative Comparison of Capture-SELEX, GO-SELEX, and Gold-SELEX for Enrichment of Aptamers

Quantitative Comparison of Capture-SELEX, GO-SELEX, and Gold-SELEX for Enrichment of Aptamers

Since 1990, numerous methods for aptamer selection have been developed, although a quantitative comparison of their sequence enrichment is lacking. In this study, we compared the enrichment factors of three library-immobilization SELEX methods (capture-SELEX, GO-SELEX, and gold-SELEX). We used a spi...

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Veröffentlicht in:Analytical chemistry (Washington) 2023-10, Vol.95 (39), p.14651-14658
Hauptverfasser: Ding, Yuzhe, Liu, Juewen
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Sprache:eng
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Adenosine
Aptamers
Deoxyribonucleic acid
DNA
Enrichment
Gold
Immobilization
Libraries
Nanoparticles
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description Since 1990, numerous methods for aptamer selection have been developed, although a quantitative comparison of their sequence enrichment is lacking. In this study, we compared the enrichment factors of three library-immobilization SELEX methods (capture-SELEX, GO-SELEX, and gold-SELEX). We used a spiked library that contained multiple DNA aptamers with different affinities for adenosine. The aptamer separation efficiency was measured using qPCR, and all of the three methods showed a very low DNA release (
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subjects Adenosine
Aptamers
Deoxyribonucleic acid
DNA
Enrichment
Gold
Immobilization
Libraries
Nanoparticles
title Quantitative Comparison of Capture-SELEX, GO-SELEX, and Gold-SELEX for Enrichment of Aptamers
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