Rapid and Highly Efficient Purification of Extracellular Vesicles Enabled by a TiO2 Hybridized Spongy-like Polymer

We developed a novel purification medium of extracellular vesicles (EVs) by constructing a spongy-like monolithic polymer kneaded with TiO2 microparticles (TiO2-hybridized spongy monolith, TiO2–SPM). TiO2–SPM was applied in a solid-phase extraction format and enabled simple, rapid, and highly effici...

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Veröffentlicht in:	Analytical chemistry (Washington) 2023-09, Vol.95 (38), p.14502-14510
Hauptverfasser:	Kanao, Eisuke, Ishida, Koki, Mizuta, Ryosuke, Li, Yuka, Imami, Koshi, Tanigawa, Tetsuya, Sasaki, Yoshihiro, Akiyoshi, Kazunari, Adachi, Jun, Otsuka, Koji, Ishihama, Yasushi, Kubo, Takuya
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Cell culture Chemistry Contamination culture media drugs Extracellular vesicles fetal bovine serum Fetal calf serum Lipid bilayers Lipids magnetism Membrane permeability Microparticles permeability Phosphatidylserine phosphatidylserines Phospholipids Polymers Pore size porosity Purification purification methods solid phase extraction Solid phases Titanium dioxide Ultracentrifugation Vesicles
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