Molecular basis and engineering of miniature Cas12f with C-rich PAM specificity

CRISPR–Cas12f nucleases are currently one of the smallest genome editors, exhibiting advantages for efficient delivery via cargo-size-limited adeno-associated virus delivery vehicles. Most characterized Cas12f nucleases recognize similar T-rich protospacer adjacent motifs (PAMs) for DNA targeting, s...

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Veröffentlicht in:	Nature chemical biology 2024-02, Vol.20 (2), p.180-189
Hauptverfasser:	Su, Mengjiao, Li, Fan, Wang, Yujue, Gao, Yan, Lan, Weiqi, Shao, Zhiwei, Zhu, Chen, Tang, Na, Gan, Jianhua, Wu, Zhaowei, Ji, Quanjiang
Format:	Artikel
Sprache:	eng
Schlagworte:	631/45/607 631/92/610 Amino acids Biochemical Engineering Biochemistry Bioorganic Chemistry Cell Biology Chemistry Chemistry and Materials Science Chemistry/Food Science Clostridium novyi CRISPR Electron microscopy Genomes Nuclease Recognition
Online-Zugang:	Volltext
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