MicroRNA-223 Inhibits Soybean Glycinin- and β‑Conglycinin-Induced Apoptosis of IPEC-J2 Cells by Targeting NLRP‑3 in the IEL/IPEC-J2 Co-culture System
The apoptosis of intestinal porcine epithelial cells induced by soybean antigen protein allergy is one of the most important mechanisms responsible for enteritis. MicroRNAs (miRNAs) affect the cellular and physiological functions of all multicellular organisms. We hypothesize that microRNA-223 inhib...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2023-09, Vol.71 (37), p.13745-13756 |
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| container_title | Journal of agricultural and food chemistry |
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| creator | Wang, Lei Sun, Zhifeng Shan, Xinggen Peng, Chenglu Ding, Hongyan Feng, Shibin Zhao, Chang Wang, Xichun Wu, Jinjie |
| description | The apoptosis of intestinal porcine epithelial cells induced by soybean antigen protein allergy is one of the most important mechanisms responsible for enteritis. MicroRNAs (miRNAs) affect the cellular and physiological functions of all multicellular organisms. We hypothesize that microRNA-223 inhibits soybean glycinin- and β-conglycinin-induced apoptosis of intestinal porcine enterocytes (IPEC-J2) by targeting the NLR family pyrin domain containing 3 (NLRP-3). Using the intestinal interepithelial lymphocyte (IEL)/IPEC-J2 co-culture system as an in vitro model, we investigate the role of microRNA-223 in the regulation of soybean glycinin- and β-conglycinin-induced apoptosis. In co-cultured IEL/IPEC-J2 cells incubated with glycinin or β-conglycinin, microRNA-223 decreased NLRP-3, ASC, caspase-1, caspase-3, FAS, BCL-2, and APAF-1 expressions in IPEC-J2 cells; decreased cytokine and cyclooxygenase-2 levels; significantly increased cell activity; and inhibited apoptosis. These data supported a novel antiallergic mechanism to mitigate the sensitization of soybean antigenic protein, which involves the upregulation of microRNA-223-targeting NLRP-3. |
| doi_str_mv | 10.1021/acs.jafc.3c01581 |
| format | Article |
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MicroRNAs (miRNAs) affect the cellular and physiological functions of all multicellular organisms. We hypothesize that microRNA-223 inhibits soybean glycinin- and β-conglycinin-induced apoptosis of intestinal porcine enterocytes (IPEC-J2) by targeting the NLR family pyrin domain containing 3 (NLRP-3). Using the intestinal interepithelial lymphocyte (IEL)/IPEC-J2 co-culture system as an in vitro model, we investigate the role of microRNA-223 in the regulation of soybean glycinin- and β-conglycinin-induced apoptosis. In co-cultured IEL/IPEC-J2 cells incubated with glycinin or β-conglycinin, microRNA-223 decreased NLRP-3, ASC, caspase-1, caspase-3, FAS, BCL-2, and APAF-1 expressions in IPEC-J2 cells; decreased cytokine and cyclooxygenase-2 levels; significantly increased cell activity; and inhibited apoptosis. These data supported a novel antiallergic mechanism to mitigate the sensitization of soybean antigenic protein, which involves the upregulation of microRNA-223-targeting NLRP-3.</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/acs.jafc.3c01581</identifier><identifier>PMID: 37682935</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Animals ; Apoptosis ; Bioactive Constituents, Metabolites, and Functions ; Coculture Techniques ; Glycine max ; Swine</subject><ispartof>Journal of agricultural and food chemistry, 2023-09, Vol.71 (37), p.13745-13756</ispartof><rights>2023 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-a289t-26a285080f8537e76dac84d11de9ad9cc797dc1093908031f4c3aaf8f3f397883</cites><orcidid>0000-0001-9706-0808 ; 0000-0003-4139-1442</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.jafc.3c01581$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.jafc.3c01581$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2763,27075,27923,27924,56737,56787</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37682935$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Lei</creatorcontrib><creatorcontrib>Sun, Zhifeng</creatorcontrib><creatorcontrib>Shan, Xinggen</creatorcontrib><creatorcontrib>Peng, Chenglu</creatorcontrib><creatorcontrib>Ding, Hongyan</creatorcontrib><creatorcontrib>Feng, Shibin</creatorcontrib><creatorcontrib>Zhao, Chang</creatorcontrib><creatorcontrib>Wang, Xichun</creatorcontrib><creatorcontrib>Wu, Jinjie</creatorcontrib><title>MicroRNA-223 Inhibits Soybean Glycinin- and β‑Conglycinin-Induced Apoptosis of IPEC-J2 Cells by Targeting NLRP‑3 in the IEL/IPEC-J2 Co-culture System</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>The apoptosis of intestinal porcine epithelial cells induced by soybean antigen protein allergy is one of the most important mechanisms responsible for enteritis. MicroRNAs (miRNAs) affect the cellular and physiological functions of all multicellular organisms. We hypothesize that microRNA-223 inhibits soybean glycinin- and β-conglycinin-induced apoptosis of intestinal porcine enterocytes (IPEC-J2) by targeting the NLR family pyrin domain containing 3 (NLRP-3). Using the intestinal interepithelial lymphocyte (IEL)/IPEC-J2 co-culture system as an in vitro model, we investigate the role of microRNA-223 in the regulation of soybean glycinin- and β-conglycinin-induced apoptosis. In co-cultured IEL/IPEC-J2 cells incubated with glycinin or β-conglycinin, microRNA-223 decreased NLRP-3, ASC, caspase-1, caspase-3, FAS, BCL-2, and APAF-1 expressions in IPEC-J2 cells; decreased cytokine and cyclooxygenase-2 levels; significantly increased cell activity; and inhibited apoptosis. These data supported a novel antiallergic mechanism to mitigate the sensitization of soybean antigenic protein, which involves the upregulation of microRNA-223-targeting NLRP-3.</description><subject>Animals</subject><subject>Apoptosis</subject><subject>Bioactive Constituents, Metabolites, and Functions</subject><subject>Coculture Techniques</subject><subject>Glycine max</subject><subject>Swine</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kbtOHDEUhq2IKCwkfSrkkiJefMEzdrkaLTDRhiAg9cjry2I0ay_jmWK6vELaPAYPwkPwJJjsQpfqSNb3_zrHHwBfCZ4STMmJ0ml6r5yeMo0JF-QDmBBOMeKEiD0wwZlBghdkHxykdI8xFrzEn8A-KwtBJeMT8PeH1128vpwhShmsw51f-j7BmzgurQrwvB21Dz4gqIKBT4_Pv_9UMazeXutgBm0NnG3ipo_JJxgdrK_mFfpOYWXbNsHlCG9Vt7K9Dyt4ubi-yhUM-gD7Owvr-eLkHY9ID20_dBbejKm368_go1Ntsl928xD8OpvfVhdo8fO8rmYLpKiQPaJFnhwL7ARnpS0Lo7Q4NYQYK5WRWpeyNJpgyWSGGHGnminlhGOOyVIIdgiOt72bLj4MNvXN2iedl1fBxiE1VBSMYcqlzCjeovnPUuqsazadX6tubAhuXo002UjzaqTZGcmRo137sFxb8x54U5CBb1vgXzQOXcjH_r_vBSDBl4Y</recordid><startdate>20230920</startdate><enddate>20230920</enddate><creator>Wang, Lei</creator><creator>Sun, Zhifeng</creator><creator>Shan, Xinggen</creator><creator>Peng, Chenglu</creator><creator>Ding, Hongyan</creator><creator>Feng, Shibin</creator><creator>Zhao, Chang</creator><creator>Wang, Xichun</creator><creator>Wu, Jinjie</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-9706-0808</orcidid><orcidid>https://orcid.org/0000-0003-4139-1442</orcidid></search><sort><creationdate>20230920</creationdate><title>MicroRNA-223 Inhibits Soybean Glycinin- and β‑Conglycinin-Induced Apoptosis of IPEC-J2 Cells by Targeting NLRP‑3 in the IEL/IPEC-J2 Co-culture System</title><author>Wang, Lei ; Sun, Zhifeng ; Shan, Xinggen ; Peng, Chenglu ; Ding, Hongyan ; Feng, Shibin ; Zhao, Chang ; Wang, Xichun ; Wu, Jinjie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a289t-26a285080f8537e76dac84d11de9ad9cc797dc1093908031f4c3aaf8f3f397883</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animals</topic><topic>Apoptosis</topic><topic>Bioactive Constituents, Metabolites, and Functions</topic><topic>Coculture Techniques</topic><topic>Glycine max</topic><topic>Swine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Lei</creatorcontrib><creatorcontrib>Sun, Zhifeng</creatorcontrib><creatorcontrib>Shan, Xinggen</creatorcontrib><creatorcontrib>Peng, Chenglu</creatorcontrib><creatorcontrib>Ding, Hongyan</creatorcontrib><creatorcontrib>Feng, Shibin</creatorcontrib><creatorcontrib>Zhao, Chang</creatorcontrib><creatorcontrib>Wang, Xichun</creatorcontrib><creatorcontrib>Wu, Jinjie</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Lei</au><au>Sun, Zhifeng</au><au>Shan, Xinggen</au><au>Peng, Chenglu</au><au>Ding, Hongyan</au><au>Feng, Shibin</au><au>Zhao, Chang</au><au>Wang, Xichun</au><au>Wu, Jinjie</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MicroRNA-223 Inhibits Soybean Glycinin- and β‑Conglycinin-Induced Apoptosis of IPEC-J2 Cells by Targeting NLRP‑3 in the IEL/IPEC-J2 Co-culture System</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2023-09-20</date><risdate>2023</risdate><volume>71</volume><issue>37</issue><spage>13745</spage><epage>13756</epage><pages>13745-13756</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><abstract>The apoptosis of intestinal porcine epithelial cells induced by soybean antigen protein allergy is one of the most important mechanisms responsible for enteritis. MicroRNAs (miRNAs) affect the cellular and physiological functions of all multicellular organisms. We hypothesize that microRNA-223 inhibits soybean glycinin- and β-conglycinin-induced apoptosis of intestinal porcine enterocytes (IPEC-J2) by targeting the NLR family pyrin domain containing 3 (NLRP-3). Using the intestinal interepithelial lymphocyte (IEL)/IPEC-J2 co-culture system as an in vitro model, we investigate the role of microRNA-223 in the regulation of soybean glycinin- and β-conglycinin-induced apoptosis. In co-cultured IEL/IPEC-J2 cells incubated with glycinin or β-conglycinin, microRNA-223 decreased NLRP-3, ASC, caspase-1, caspase-3, FAS, BCL-2, and APAF-1 expressions in IPEC-J2 cells; decreased cytokine and cyclooxygenase-2 levels; significantly increased cell activity; and inhibited apoptosis. These data supported a novel antiallergic mechanism to mitigate the sensitization of soybean antigenic protein, which involves the upregulation of microRNA-223-targeting NLRP-3.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>37682935</pmid><doi>10.1021/acs.jafc.3c01581</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0001-9706-0808</orcidid><orcidid>https://orcid.org/0000-0003-4139-1442</orcidid></addata></record> |
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| subjects | Animals Apoptosis Bioactive Constituents, Metabolites, and Functions Coculture Techniques Glycine max Swine |
| title | MicroRNA-223 Inhibits Soybean Glycinin- and β‑Conglycinin-Induced Apoptosis of IPEC-J2 Cells by Targeting NLRP‑3 in the IEL/IPEC-J2 Co-culture System |
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