BCp12/PLA combination: A novel antibacterial agent targeting Mur family, DNA gyrase and DHFR
The combination of natural antimicrobial peptide BCp12/phenyllatic acid (BCp12/PLA) presents a more efficient antibacterial effect, but its antibacterial mechanism remains unclear. This study studied the synergistic antibacterial mechanism of BCp12 and PLA against S. aureus. The results demonstrated...
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| Veröffentlicht in: | International journal of food microbiology 2023-12, Vol.406, p.110370-110370, Article 110370 |
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| container_title | International journal of food microbiology |
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| creator | Li, Yufang Tang, Xiaozhao Yang, Zushun He, Jinze Ma, Ni Huang, Aixiang Shi, Yanan |
| description | The combination of natural antimicrobial peptide BCp12/phenyllatic acid (BCp12/PLA) presents a more efficient antibacterial effect, but its antibacterial mechanism remains unclear. This study studied the synergistic antibacterial mechanism of BCp12 and PLA against S. aureus. The results demonstrated that the BCp12/PLA combination presented a synergistic antibacterial effect against S. aureus, with a fractional inhibitory concentration of 0.05. Furthermore, flow cytometry and scanning electron microscope analysis revealed that BCp12 and PLA synergistically promoted cell membrane disruption compared with the group treated only with one compound, inducing structural cell damage and cytoplasmic leakage. In addition, fluorescence spectroscopy analysis suggested that BCp12 and PLA synergistically influenced genomic DNA. BCp12 and PLA targeted enzymes related to peptidoglycan and DNA synthesis and interacted by hydrogen bonding and hydrophobic interactions with mur enzymes (murC, murD, murE, murF, and murG), dihydrofolate reductase, and DNA gyrase. Additionally, the combined treatment successfully inhibited microbial reproduction in the storage of pasteurized milk, indicating that the combination of BCp12 and PLA can be used as a new preservative strategy in food systems. Overall, this study could provide potential strategies for preventing and controlling foodborne pathogens.
•BCp12 combined with PLA has synergistic antibacterial effect.•BCp12 combined with PLA destroyed the integrity and permeability of cell wall membrane.•BCp12 combined with PLA interfered with the synthesis of peptidoglycan.•BCp12 combined with PLA affected DNA synthesis and self-replication.•The BCp12 combined with PLA is a new and efficient food preservative. |
| doi_str_mv | 10.1016/j.ijfoodmicro.2023.110370 |
| format | Article |
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•BCp12 combined with PLA has synergistic antibacterial effect.•BCp12 combined with PLA destroyed the integrity and permeability of cell wall membrane.•BCp12 combined with PLA interfered with the synthesis of peptidoglycan.•BCp12 combined with PLA affected DNA synthesis and self-replication.•The BCp12 combined with PLA is a new and efficient food preservative.</description><identifier>ISSN: 0168-1605</identifier><identifier>EISSN: 1879-3460</identifier><identifier>DOI: 10.1016/j.ijfoodmicro.2023.110370</identifier><language>eng</language><publisher>Elsevier B.V</publisher><subject>antibacterial properties ; antibiotics ; antimicrobial peptides ; BCp 12 ; cell membranes ; dihydrofolate reductase ; DNA ; DNA replication ; DNA topoisomerase (ATP-hydrolysing) ; family ; flow cytometry ; fluorescence emission spectroscopy ; food microbiology ; hydrogen ; hydrophobicity ; Mur enzyme ; pasteurized milk ; peptidoglycans ; PLA ; reproduction ; Staphylococcus aureus ; Synergistic antibacterial</subject><ispartof>International journal of food microbiology, 2023-12, Vol.406, p.110370-110370, Article 110370</ispartof><rights>2023 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c387t-54ce2b76819275ca4d26736ccf0da71668c01d21f69720b704937156394e0ec3</citedby><cites>FETCH-LOGICAL-c387t-54ce2b76819275ca4d26736ccf0da71668c01d21f69720b704937156394e0ec3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ijfoodmicro.2023.110370$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27911,27912,45982</link.rule.ids></links><search><creatorcontrib>Li, Yufang</creatorcontrib><creatorcontrib>Tang, Xiaozhao</creatorcontrib><creatorcontrib>Yang, Zushun</creatorcontrib><creatorcontrib>He, Jinze</creatorcontrib><creatorcontrib>Ma, Ni</creatorcontrib><creatorcontrib>Huang, Aixiang</creatorcontrib><creatorcontrib>Shi, Yanan</creatorcontrib><title>BCp12/PLA combination: A novel antibacterial agent targeting Mur family, DNA gyrase and DHFR</title><title>International journal of food microbiology</title><description>The combination of natural antimicrobial peptide BCp12/phenyllatic acid (BCp12/PLA) presents a more efficient antibacterial effect, but its antibacterial mechanism remains unclear. This study studied the synergistic antibacterial mechanism of BCp12 and PLA against S. aureus. The results demonstrated that the BCp12/PLA combination presented a synergistic antibacterial effect against S. aureus, with a fractional inhibitory concentration of 0.05. Furthermore, flow cytometry and scanning electron microscope analysis revealed that BCp12 and PLA synergistically promoted cell membrane disruption compared with the group treated only with one compound, inducing structural cell damage and cytoplasmic leakage. In addition, fluorescence spectroscopy analysis suggested that BCp12 and PLA synergistically influenced genomic DNA. BCp12 and PLA targeted enzymes related to peptidoglycan and DNA synthesis and interacted by hydrogen bonding and hydrophobic interactions with mur enzymes (murC, murD, murE, murF, and murG), dihydrofolate reductase, and DNA gyrase. Additionally, the combined treatment successfully inhibited microbial reproduction in the storage of pasteurized milk, indicating that the combination of BCp12 and PLA can be used as a new preservative strategy in food systems. Overall, this study could provide potential strategies for preventing and controlling foodborne pathogens.
•BCp12 combined with PLA has synergistic antibacterial effect.•BCp12 combined with PLA destroyed the integrity and permeability of cell wall membrane.•BCp12 combined with PLA interfered with the synthesis of peptidoglycan.•BCp12 combined with PLA affected DNA synthesis and self-replication.•The BCp12 combined with PLA is a new and efficient food preservative.</description><subject>antibacterial properties</subject><subject>antibiotics</subject><subject>antimicrobial peptides</subject><subject>BCp 12</subject><subject>cell membranes</subject><subject>dihydrofolate reductase</subject><subject>DNA</subject><subject>DNA replication</subject><subject>DNA topoisomerase (ATP-hydrolysing)</subject><subject>family</subject><subject>flow cytometry</subject><subject>fluorescence emission spectroscopy</subject><subject>food microbiology</subject><subject>hydrogen</subject><subject>hydrophobicity</subject><subject>Mur enzyme</subject><subject>pasteurized milk</subject><subject>peptidoglycans</subject><subject>PLA</subject><subject>reproduction</subject><subject>Staphylococcus aureus</subject><subject>Synergistic antibacterial</subject><issn>0168-1605</issn><issn>1879-3460</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNqNkE1P4zAURS00SHSA_2B2LEh5thM7nl1p-RipAwixRLJc56VylcTFdpH67wnqLGbHrJ6udO6V3iHkgsGUAZPXm6nftCE0vXcxTDlwMWUMhIIjMmG10oUoJfwgk5GtCyahOiE_U9oAQCUETMjbzXzL-PXzckZd6Fd-sNmH4Red0SF8YEftkP3KuozR2zGtccg027jG7Ic1_bOLtLW97_ZXdPE4o-t9tAnHUkMXD3cvZ-S4tV3C87_3lLze3b7OH4rl0_3v-WxZOFGrXFSlQ75Ssmaaq8rZsuFSCelcC41VTMraAWs4a6VWHFYKSi0Uq6TQJQI6cUouD7PbGN53mLLpfXLYdXbAsEtGsEpUuiq1_hbltRRcawZqRPUBHcWmFLE12-h7G_eGgflybzbmH_fmy705uB-780MXx68_PEaTnMfBYeMjumya4P9j5RPZb5AC</recordid><startdate>20231202</startdate><enddate>20231202</enddate><creator>Li, Yufang</creator><creator>Tang, Xiaozhao</creator><creator>Yang, Zushun</creator><creator>He, Jinze</creator><creator>Ma, Ni</creator><creator>Huang, Aixiang</creator><creator>Shi, Yanan</creator><general>Elsevier B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20231202</creationdate><title>BCp12/PLA combination: A novel antibacterial agent targeting Mur family, DNA gyrase and DHFR</title><author>Li, Yufang ; Tang, Xiaozhao ; Yang, Zushun ; He, Jinze ; Ma, Ni ; Huang, Aixiang ; Shi, Yanan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c387t-54ce2b76819275ca4d26736ccf0da71668c01d21f69720b704937156394e0ec3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>antibacterial properties</topic><topic>antibiotics</topic><topic>antimicrobial peptides</topic><topic>BCp 12</topic><topic>cell membranes</topic><topic>dihydrofolate reductase</topic><topic>DNA</topic><topic>DNA replication</topic><topic>DNA topoisomerase (ATP-hydrolysing)</topic><topic>family</topic><topic>flow cytometry</topic><topic>fluorescence emission spectroscopy</topic><topic>food microbiology</topic><topic>hydrogen</topic><topic>hydrophobicity</topic><topic>Mur enzyme</topic><topic>pasteurized milk</topic><topic>peptidoglycans</topic><topic>PLA</topic><topic>reproduction</topic><topic>Staphylococcus aureus</topic><topic>Synergistic antibacterial</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Yufang</creatorcontrib><creatorcontrib>Tang, Xiaozhao</creatorcontrib><creatorcontrib>Yang, Zushun</creatorcontrib><creatorcontrib>He, Jinze</creatorcontrib><creatorcontrib>Ma, Ni</creatorcontrib><creatorcontrib>Huang, Aixiang</creatorcontrib><creatorcontrib>Shi, Yanan</creatorcontrib><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>International journal of food microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Yufang</au><au>Tang, Xiaozhao</au><au>Yang, Zushun</au><au>He, Jinze</au><au>Ma, Ni</au><au>Huang, Aixiang</au><au>Shi, Yanan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>BCp12/PLA combination: A novel antibacterial agent targeting Mur family, DNA gyrase and DHFR</atitle><jtitle>International journal of food microbiology</jtitle><date>2023-12-02</date><risdate>2023</risdate><volume>406</volume><spage>110370</spage><epage>110370</epage><pages>110370-110370</pages><artnum>110370</artnum><issn>0168-1605</issn><eissn>1879-3460</eissn><abstract>The combination of natural antimicrobial peptide BCp12/phenyllatic acid (BCp12/PLA) presents a more efficient antibacterial effect, but its antibacterial mechanism remains unclear. This study studied the synergistic antibacterial mechanism of BCp12 and PLA against S. aureus. The results demonstrated that the BCp12/PLA combination presented a synergistic antibacterial effect against S. aureus, with a fractional inhibitory concentration of 0.05. Furthermore, flow cytometry and scanning electron microscope analysis revealed that BCp12 and PLA synergistically promoted cell membrane disruption compared with the group treated only with one compound, inducing structural cell damage and cytoplasmic leakage. In addition, fluorescence spectroscopy analysis suggested that BCp12 and PLA synergistically influenced genomic DNA. BCp12 and PLA targeted enzymes related to peptidoglycan and DNA synthesis and interacted by hydrogen bonding and hydrophobic interactions with mur enzymes (murC, murD, murE, murF, and murG), dihydrofolate reductase, and DNA gyrase. Additionally, the combined treatment successfully inhibited microbial reproduction in the storage of pasteurized milk, indicating that the combination of BCp12 and PLA can be used as a new preservative strategy in food systems. Overall, this study could provide potential strategies for preventing and controlling foodborne pathogens.
•BCp12 combined with PLA has synergistic antibacterial effect.•BCp12 combined with PLA destroyed the integrity and permeability of cell wall membrane.•BCp12 combined with PLA interfered with the synthesis of peptidoglycan.•BCp12 combined with PLA affected DNA synthesis and self-replication.•The BCp12 combined with PLA is a new and efficient food preservative.</abstract><pub>Elsevier B.V</pub><doi>10.1016/j.ijfoodmicro.2023.110370</doi><tpages>1</tpages></addata></record> |
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| source | ScienceDirect Journals (5 years ago - present) |
| subjects | antibacterial properties antibiotics antimicrobial peptides BCp 12 cell membranes dihydrofolate reductase DNA DNA replication DNA topoisomerase (ATP-hydrolysing) family flow cytometry fluorescence emission spectroscopy food microbiology hydrogen hydrophobicity Mur enzyme pasteurized milk peptidoglycans PLA reproduction Staphylococcus aureus Synergistic antibacterial |
| title | BCp12/PLA combination: A novel antibacterial agent targeting Mur family, DNA gyrase and DHFR |
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