Role of Extracellular Vesicles in Absorption and Functional Mechanisms of Quercetin
ScopeQuercetin (QUE), a phytochemical found in various plant foods, has been shown to have a variety of physiological activities in vivo, though biological sites where it has activities and the mechanisms of transport have not been fully elucidated.Methods and resultsIn the present study, intracellu...
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| Veröffentlicht in: | Molecular nutrition & food research 2023-11, Vol.67 (21), p.e2300225-e2300225 |
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| container_issue | 21 |
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| container_title | Molecular nutrition & food research |
| container_volume | 67 |
| creator | Ishisaka, Akari Sugimoto, Ryosuke Marumo, Haruka Doi, Tomoki Hamada, Kaede Fujimoto, Misa Fujiwara, Nao Yamasaki, Masao Murakami, Akira |
| description | ScopeQuercetin (QUE), a phytochemical found in various plant foods, has been shown to have a variety of physiological activities in vivo, though biological sites where it has activities and the mechanisms of transport have not been fully elucidated.Methods and resultsIn the present study, intracellular uptake of QUE into HT‐29 human colon adenocarcinoma cells is found to result in spontaneous release of extracellular vesicles (EVs), which are subsequently embedded with QUE. In addition, QUE‐embedded EVs are detected in serum of QUE‐administered Sprague‐Dawley rats. Interestingly, the rate of cellular uptake of QUE‐encapsulated EVs (EV‐QUE) into RAW264.7 macrophages is markedly higher than that of free QUE. Moreover, EV‐QUE suppresses lipopolysaccharide (LPS)‐induced nitric oxide at a lower concentration than free QUE.ConclusionThe present findings suggest that QUE may be embedded in EVs in the gastrointestinal tract, then become absorbed and enter the bloodstream to exhibit biological activities. |
| doi_str_mv | 10.1002/mnfr.202300225 |
| format | Article |
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In addition, QUE‐embedded EVs are detected in serum of QUE‐administered Sprague‐Dawley rats. Interestingly, the rate of cellular uptake of QUE‐encapsulated EVs (EV‐QUE) into RAW264.7 macrophages is markedly higher than that of free QUE. Moreover, EV‐QUE suppresses lipopolysaccharide (LPS)‐induced nitric oxide at a lower concentration than free QUE.ConclusionThe present findings suggest that QUE may be embedded in EVs in the gastrointestinal tract, then become absorbed and enter the bloodstream to exhibit biological activities.</description><identifier>ISSN: 1613-4125</identifier><identifier>EISSN: 1613-4133</identifier><identifier>DOI: 10.1002/mnfr.202300225</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc</publisher><subject>Adenocarcinoma ; Extracellular vesicles ; Gastrointestinal system ; Gastrointestinal tract ; Lipopolysaccharides ; Macrophages ; Nitric oxide ; Quercetin ; Vesicles</subject><ispartof>Molecular nutrition & food research, 2023-11, Vol.67 (21), p.e2300225-e2300225</ispartof><rights>2023 Wiley‐VCH GmbH</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c295t-befef4edd9b2c034f1def3d273d2bd4b1a7a76417d8dffc13bc39253ebce4aa23</cites><orcidid>0000-0001-7758-9405 ; 0000-0002-5694-2828</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27907,27908</link.rule.ids></links><search><creatorcontrib>Ishisaka, Akari</creatorcontrib><creatorcontrib>Sugimoto, Ryosuke</creatorcontrib><creatorcontrib>Marumo, Haruka</creatorcontrib><creatorcontrib>Doi, Tomoki</creatorcontrib><creatorcontrib>Hamada, Kaede</creatorcontrib><creatorcontrib>Fujimoto, Misa</creatorcontrib><creatorcontrib>Fujiwara, Nao</creatorcontrib><creatorcontrib>Yamasaki, Masao</creatorcontrib><creatorcontrib>Murakami, Akira</creatorcontrib><title>Role of Extracellular Vesicles in Absorption and Functional Mechanisms of Quercetin</title><title>Molecular nutrition & food research</title><description>ScopeQuercetin (QUE), a phytochemical found in various plant foods, has been shown to have a variety of physiological activities in vivo, though biological sites where it has activities and the mechanisms of transport have not been fully elucidated.Methods and resultsIn the present study, intracellular uptake of QUE into HT‐29 human colon adenocarcinoma cells is found to result in spontaneous release of extracellular vesicles (EVs), which are subsequently embedded with QUE. In addition, QUE‐embedded EVs are detected in serum of QUE‐administered Sprague‐Dawley rats. Interestingly, the rate of cellular uptake of QUE‐encapsulated EVs (EV‐QUE) into RAW264.7 macrophages is markedly higher than that of free QUE. Moreover, EV‐QUE suppresses lipopolysaccharide (LPS)‐induced nitric oxide at a lower concentration than free QUE.ConclusionThe present findings suggest that QUE may be embedded in EVs in the gastrointestinal tract, then become absorbed and enter the bloodstream to exhibit biological activities.</description><subject>Adenocarcinoma</subject><subject>Extracellular vesicles</subject><subject>Gastrointestinal system</subject><subject>Gastrointestinal tract</subject><subject>Lipopolysaccharides</subject><subject>Macrophages</subject><subject>Nitric oxide</subject><subject>Quercetin</subject><subject>Vesicles</subject><issn>1613-4125</issn><issn>1613-4133</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNpdkE1LAzEQhoMoWKtXzwEvXrbma7vbYymtChXx8xqyyQS3ZJOa7IL-ezdUPHgYZl54eGEehC4pmVFC2E3nbZwxwvgYWHmEJnROeSEo58d_NytP0VlKO0I4ZYJP0MtzcICDxeuvPioNzg1ORfwOqdUOEm49XjYpxH3fBo-VN3gzeJ2DcvgB9IfybepSbngaIGroW3-OTqxyCS5-9xS9bdavq7ti-3h7v1puC80WZV80YMEKMGbRME24sNSA5YZV4zRGNFRVqpoLWpnaWKspbzRfsJJDo0EoxfgUXR969zF8DpB62bUpv6A8hCFJVs_Z6IPSjF79Q3dhiOMPmarLqixrUY_U7EDpGFKKYOU-tp2K35ISmR3L7Fj-OeY_Hz1wzA</recordid><startdate>20231101</startdate><enddate>20231101</enddate><creator>Ishisaka, Akari</creator><creator>Sugimoto, Ryosuke</creator><creator>Marumo, Haruka</creator><creator>Doi, Tomoki</creator><creator>Hamada, Kaede</creator><creator>Fujimoto, Misa</creator><creator>Fujiwara, Nao</creator><creator>Yamasaki, Masao</creator><creator>Murakami, Akira</creator><general>Wiley Subscription Services, Inc</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>7T5</scope><scope>7T7</scope><scope>7TK</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-7758-9405</orcidid><orcidid>https://orcid.org/0000-0002-5694-2828</orcidid></search><sort><creationdate>20231101</creationdate><title>Role of Extracellular Vesicles in Absorption and Functional Mechanisms of Quercetin</title><author>Ishisaka, Akari ; Sugimoto, Ryosuke ; Marumo, Haruka ; Doi, Tomoki ; Hamada, Kaede ; Fujimoto, Misa ; Fujiwara, Nao ; Yamasaki, Masao ; Murakami, Akira</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c295t-befef4edd9b2c034f1def3d273d2bd4b1a7a76417d8dffc13bc39253ebce4aa23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Adenocarcinoma</topic><topic>Extracellular vesicles</topic><topic>Gastrointestinal system</topic><topic>Gastrointestinal tract</topic><topic>Lipopolysaccharides</topic><topic>Macrophages</topic><topic>Nitric oxide</topic><topic>Quercetin</topic><topic>Vesicles</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ishisaka, Akari</creatorcontrib><creatorcontrib>Sugimoto, Ryosuke</creatorcontrib><creatorcontrib>Marumo, Haruka</creatorcontrib><creatorcontrib>Doi, Tomoki</creatorcontrib><creatorcontrib>Hamada, Kaede</creatorcontrib><creatorcontrib>Fujimoto, Misa</creatorcontrib><creatorcontrib>Fujiwara, Nao</creatorcontrib><creatorcontrib>Yamasaki, Masao</creatorcontrib><creatorcontrib>Murakami, Akira</creatorcontrib><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular nutrition & food research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ishisaka, Akari</au><au>Sugimoto, Ryosuke</au><au>Marumo, Haruka</au><au>Doi, Tomoki</au><au>Hamada, Kaede</au><au>Fujimoto, Misa</au><au>Fujiwara, Nao</au><au>Yamasaki, Masao</au><au>Murakami, Akira</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of Extracellular Vesicles in Absorption and Functional Mechanisms of Quercetin</atitle><jtitle>Molecular nutrition & food research</jtitle><date>2023-11-01</date><risdate>2023</risdate><volume>67</volume><issue>21</issue><spage>e2300225</spage><epage>e2300225</epage><pages>e2300225-e2300225</pages><issn>1613-4125</issn><eissn>1613-4133</eissn><abstract>ScopeQuercetin (QUE), a phytochemical found in various plant foods, has been shown to have a variety of physiological activities in vivo, though biological sites where it has activities and the mechanisms of transport have not been fully elucidated.Methods and resultsIn the present study, intracellular uptake of QUE into HT‐29 human colon adenocarcinoma cells is found to result in spontaneous release of extracellular vesicles (EVs), which are subsequently embedded with QUE. In addition, QUE‐embedded EVs are detected in serum of QUE‐administered Sprague‐Dawley rats. Interestingly, the rate of cellular uptake of QUE‐encapsulated EVs (EV‐QUE) into RAW264.7 macrophages is markedly higher than that of free QUE. Moreover, EV‐QUE suppresses lipopolysaccharide (LPS)‐induced nitric oxide at a lower concentration than free QUE.ConclusionThe present findings suggest that QUE may be embedded in EVs in the gastrointestinal tract, then become absorbed and enter the bloodstream to exhibit biological activities.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc</pub><doi>10.1002/mnfr.202300225</doi><orcidid>https://orcid.org/0000-0001-7758-9405</orcidid><orcidid>https://orcid.org/0000-0002-5694-2828</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | Adenocarcinoma Extracellular vesicles Gastrointestinal system Gastrointestinal tract Lipopolysaccharides Macrophages Nitric oxide Quercetin Vesicles |
| title | Role of Extracellular Vesicles in Absorption and Functional Mechanisms of Quercetin |
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