A specific plasma amino acid profile in the Insulin2Q104del Kuma mice at the diabetic state and reversal from hyperglycemia
The metabolites in the plasma serve as potential biomarkers of disease. We previously established an early-onset diabetes mouse model, Ins2+/Q104del Kuma mice, under a severe immune-deficient (Rag-2/Jak3 double-deficient in BALB/c) background. Here, we revealed the differences in plasma amino acid p...
Gespeichert in:
Veröffentlicht in: | Biochemical and biophysical research communications 2023-10, Vol.679, p.58-65 |
---|---|
Hauptverfasser: | , , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 65 |
---|---|
container_issue | |
container_start_page | 58 |
container_title | Biochemical and biophysical research communications |
container_volume | 679 |
creator | Hiyoshi, Naoya Enomoto, Takayuki Uefune, Fumiya Kato, Yusuke Wu, Yumeng Araki, Kimi Sakano, Daisuke Shiraki, Nobuaki Kume, Shoen |
description | The metabolites in the plasma serve as potential biomarkers of disease. We previously established an early-onset diabetes mouse model, Ins2+/Q104del Kuma mice, under a severe immune-deficient (Rag-2/Jak3 double-deficient in BALB/c) background. Here, we revealed the differences in plasma amino acid profiles between Kuma and the wild-type mice. We observed an early reduction in glucogenic and ketogenic amino acids, a late increase in branched-chain amino acids (BCAAs) and succinyl CoA-related amino acids, and a trend of increasing ketogenic amino acids in Kuma mice than in the wild-type mice. Kuma mice exhibited hyperglucagonemia at high blood glucose, leading to perturbations in plasma amino acid profiles. The reversal of blood glucose by islet transplantation normalized the increases of the BCAAs and several aspects of the altered metabolic profiles in Kuma mice. Our results indicate that the Kuma mice are a unique animal model to study the link between plasma amino acid profile and the progression of diabetes for monitoring the therapeutic effects.
•The diabetic Ins2+/Q104del Kuma mice showed characteristic plasma amino acid profiles.•Kuma mice showed an early reduction in glucogenic and ketogenic amino acids.•A late increase in branched-chain amino acids (BCAAs) was observed in the Kuma mice.•Islet transplantation corrected blood glucose and metabolic profile to some extent.•Increases in plasma glucagon in Kuma mice may explain the elevated BCAAs. |
doi_str_mv | 10.1016/j.bbrc.2023.08.064 |
format | Article |
fullrecord | <record><control><sourceid>proquest_elsev</sourceid><recordid>TN_cdi_proquest_miscellaneous_2862201984</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0006291X23010264</els_id><sourcerecordid>2862201984</sourcerecordid><originalsourceid>FETCH-LOGICAL-e864-d97127db33d7e10a9cda67da2ad90b9c0eb7a33ba7076130dfa660e51517ca5d3</originalsourceid><addsrcrecordid>eNotkE1Lw0AQQBdRsFb_gKc9ekmc3aSbBLyU4kexIEIP3pbJ7sRu2XyYTQrFP2-qngaGx2PmMXYrIBYg1P0-LsvexBJkEkMeg0rP2ExAAZEUkJ6zGQCoSBbi45JdhbAHECJVxYx9L3noyLjKGd55DDVyrF3TcjTO8q5vK-eJu4YPO-LrJozeNfJ9clry_HWc8NoZ4jj8AtZhScOkCgMO07axvKcD9QE9r_q25rtjR_2nPxqqHV6ziwp9oJv_OWfbp8ft6iXavD2vV8tNRLlKI1tkQma2TBKbkQAsjEWVWZRoCygLA1RmmCQlZpApkYCtUCmghViIzODCJnN296edvvkaKQy6dsGQ99hQOwYtcyUliCJPJ_ThD6XpnIOjXgfjqDFkXU9m0LZ1WoA-Jdd7fUquT8k15HpKnvwA86h3ZQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2862201984</pqid></control><display><type>article</type><title>A specific plasma amino acid profile in the Insulin2Q104del Kuma mice at the diabetic state and reversal from hyperglycemia</title><source>Elsevier ScienceDirect Journals Complete</source><creator>Hiyoshi, Naoya ; Enomoto, Takayuki ; Uefune, Fumiya ; Kato, Yusuke ; Wu, Yumeng ; Araki, Kimi ; Sakano, Daisuke ; Shiraki, Nobuaki ; Kume, Shoen</creator><creatorcontrib>Hiyoshi, Naoya ; Enomoto, Takayuki ; Uefune, Fumiya ; Kato, Yusuke ; Wu, Yumeng ; Araki, Kimi ; Sakano, Daisuke ; Shiraki, Nobuaki ; Kume, Shoen</creatorcontrib><description>The metabolites in the plasma serve as potential biomarkers of disease. We previously established an early-onset diabetes mouse model, Ins2+/Q104del Kuma mice, under a severe immune-deficient (Rag-2/Jak3 double-deficient in BALB/c) background. Here, we revealed the differences in plasma amino acid profiles between Kuma and the wild-type mice. We observed an early reduction in glucogenic and ketogenic amino acids, a late increase in branched-chain amino acids (BCAAs) and succinyl CoA-related amino acids, and a trend of increasing ketogenic amino acids in Kuma mice than in the wild-type mice. Kuma mice exhibited hyperglucagonemia at high blood glucose, leading to perturbations in plasma amino acid profiles. The reversal of blood glucose by islet transplantation normalized the increases of the BCAAs and several aspects of the altered metabolic profiles in Kuma mice. Our results indicate that the Kuma mice are a unique animal model to study the link between plasma amino acid profile and the progression of diabetes for monitoring the therapeutic effects.
•The diabetic Ins2+/Q104del Kuma mice showed characteristic plasma amino acid profiles.•Kuma mice showed an early reduction in glucogenic and ketogenic amino acids.•A late increase in branched-chain amino acids (BCAAs) was observed in the Kuma mice.•Islet transplantation corrected blood glucose and metabolic profile to some extent.•Increases in plasma glucagon in Kuma mice may explain the elevated BCAAs.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2023.08.064</identifier><language>eng</language><publisher>Elsevier Inc</publisher><subject>Diabetes ; Insulin mutation ; Metabolite ; Mouse ; Plasma amino acid profile</subject><ispartof>Biochemical and biophysical research communications, 2023-10, Vol.679, p.58-65</ispartof><rights>2023 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0000-0001-5305-5556 ; 0000-0002-1396-1527 ; 0009-0002-0699-1768 ; 0000-0002-4292-205X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bbrc.2023.08.064$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids></links><search><creatorcontrib>Hiyoshi, Naoya</creatorcontrib><creatorcontrib>Enomoto, Takayuki</creatorcontrib><creatorcontrib>Uefune, Fumiya</creatorcontrib><creatorcontrib>Kato, Yusuke</creatorcontrib><creatorcontrib>Wu, Yumeng</creatorcontrib><creatorcontrib>Araki, Kimi</creatorcontrib><creatorcontrib>Sakano, Daisuke</creatorcontrib><creatorcontrib>Shiraki, Nobuaki</creatorcontrib><creatorcontrib>Kume, Shoen</creatorcontrib><title>A specific plasma amino acid profile in the Insulin2Q104del Kuma mice at the diabetic state and reversal from hyperglycemia</title><title>Biochemical and biophysical research communications</title><description>The metabolites in the plasma serve as potential biomarkers of disease. We previously established an early-onset diabetes mouse model, Ins2+/Q104del Kuma mice, under a severe immune-deficient (Rag-2/Jak3 double-deficient in BALB/c) background. Here, we revealed the differences in plasma amino acid profiles between Kuma and the wild-type mice. We observed an early reduction in glucogenic and ketogenic amino acids, a late increase in branched-chain amino acids (BCAAs) and succinyl CoA-related amino acids, and a trend of increasing ketogenic amino acids in Kuma mice than in the wild-type mice. Kuma mice exhibited hyperglucagonemia at high blood glucose, leading to perturbations in plasma amino acid profiles. The reversal of blood glucose by islet transplantation normalized the increases of the BCAAs and several aspects of the altered metabolic profiles in Kuma mice. Our results indicate that the Kuma mice are a unique animal model to study the link between plasma amino acid profile and the progression of diabetes for monitoring the therapeutic effects.
•The diabetic Ins2+/Q104del Kuma mice showed characteristic plasma amino acid profiles.•Kuma mice showed an early reduction in glucogenic and ketogenic amino acids.•A late increase in branched-chain amino acids (BCAAs) was observed in the Kuma mice.•Islet transplantation corrected blood glucose and metabolic profile to some extent.•Increases in plasma glucagon in Kuma mice may explain the elevated BCAAs.</description><subject>Diabetes</subject><subject>Insulin mutation</subject><subject>Metabolite</subject><subject>Mouse</subject><subject>Plasma amino acid profile</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNotkE1Lw0AQQBdRsFb_gKc9ekmc3aSbBLyU4kexIEIP3pbJ7sRu2XyYTQrFP2-qngaGx2PmMXYrIBYg1P0-LsvexBJkEkMeg0rP2ExAAZEUkJ6zGQCoSBbi45JdhbAHECJVxYx9L3noyLjKGd55DDVyrF3TcjTO8q5vK-eJu4YPO-LrJozeNfJ9clry_HWc8NoZ4jj8AtZhScOkCgMO07axvKcD9QE9r_q25rtjR_2nPxqqHV6ziwp9oJv_OWfbp8ft6iXavD2vV8tNRLlKI1tkQma2TBKbkQAsjEWVWZRoCygLA1RmmCQlZpApkYCtUCmghViIzODCJnN296edvvkaKQy6dsGQ99hQOwYtcyUliCJPJ_ThD6XpnIOjXgfjqDFkXU9m0LZ1WoA-Jdd7fUquT8k15HpKnvwA86h3ZQ</recordid><startdate>20231030</startdate><enddate>20231030</enddate><creator>Hiyoshi, Naoya</creator><creator>Enomoto, Takayuki</creator><creator>Uefune, Fumiya</creator><creator>Kato, Yusuke</creator><creator>Wu, Yumeng</creator><creator>Araki, Kimi</creator><creator>Sakano, Daisuke</creator><creator>Shiraki, Nobuaki</creator><creator>Kume, Shoen</creator><general>Elsevier Inc</general><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-5305-5556</orcidid><orcidid>https://orcid.org/0000-0002-1396-1527</orcidid><orcidid>https://orcid.org/0009-0002-0699-1768</orcidid><orcidid>https://orcid.org/0000-0002-4292-205X</orcidid></search><sort><creationdate>20231030</creationdate><title>A specific plasma amino acid profile in the Insulin2Q104del Kuma mice at the diabetic state and reversal from hyperglycemia</title><author>Hiyoshi, Naoya ; Enomoto, Takayuki ; Uefune, Fumiya ; Kato, Yusuke ; Wu, Yumeng ; Araki, Kimi ; Sakano, Daisuke ; Shiraki, Nobuaki ; Kume, Shoen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e864-d97127db33d7e10a9cda67da2ad90b9c0eb7a33ba7076130dfa660e51517ca5d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Diabetes</topic><topic>Insulin mutation</topic><topic>Metabolite</topic><topic>Mouse</topic><topic>Plasma amino acid profile</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hiyoshi, Naoya</creatorcontrib><creatorcontrib>Enomoto, Takayuki</creatorcontrib><creatorcontrib>Uefune, Fumiya</creatorcontrib><creatorcontrib>Kato, Yusuke</creatorcontrib><creatorcontrib>Wu, Yumeng</creatorcontrib><creatorcontrib>Araki, Kimi</creatorcontrib><creatorcontrib>Sakano, Daisuke</creatorcontrib><creatorcontrib>Shiraki, Nobuaki</creatorcontrib><creatorcontrib>Kume, Shoen</creatorcontrib><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hiyoshi, Naoya</au><au>Enomoto, Takayuki</au><au>Uefune, Fumiya</au><au>Kato, Yusuke</au><au>Wu, Yumeng</au><au>Araki, Kimi</au><au>Sakano, Daisuke</au><au>Shiraki, Nobuaki</au><au>Kume, Shoen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A specific plasma amino acid profile in the Insulin2Q104del Kuma mice at the diabetic state and reversal from hyperglycemia</atitle><jtitle>Biochemical and biophysical research communications</jtitle><date>2023-10-30</date><risdate>2023</risdate><volume>679</volume><spage>58</spage><epage>65</epage><pages>58-65</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>The metabolites in the plasma serve as potential biomarkers of disease. We previously established an early-onset diabetes mouse model, Ins2+/Q104del Kuma mice, under a severe immune-deficient (Rag-2/Jak3 double-deficient in BALB/c) background. Here, we revealed the differences in plasma amino acid profiles between Kuma and the wild-type mice. We observed an early reduction in glucogenic and ketogenic amino acids, a late increase in branched-chain amino acids (BCAAs) and succinyl CoA-related amino acids, and a trend of increasing ketogenic amino acids in Kuma mice than in the wild-type mice. Kuma mice exhibited hyperglucagonemia at high blood glucose, leading to perturbations in plasma amino acid profiles. The reversal of blood glucose by islet transplantation normalized the increases of the BCAAs and several aspects of the altered metabolic profiles in Kuma mice. Our results indicate that the Kuma mice are a unique animal model to study the link between plasma amino acid profile and the progression of diabetes for monitoring the therapeutic effects.
•The diabetic Ins2+/Q104del Kuma mice showed characteristic plasma amino acid profiles.•Kuma mice showed an early reduction in glucogenic and ketogenic amino acids.•A late increase in branched-chain amino acids (BCAAs) was observed in the Kuma mice.•Islet transplantation corrected blood glucose and metabolic profile to some extent.•Increases in plasma glucagon in Kuma mice may explain the elevated BCAAs.</abstract><pub>Elsevier Inc</pub><doi>10.1016/j.bbrc.2023.08.064</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0001-5305-5556</orcidid><orcidid>https://orcid.org/0000-0002-1396-1527</orcidid><orcidid>https://orcid.org/0009-0002-0699-1768</orcidid><orcidid>https://orcid.org/0000-0002-4292-205X</orcidid></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0006-291X |
ispartof | Biochemical and biophysical research communications, 2023-10, Vol.679, p.58-65 |
issn | 0006-291X 1090-2104 |
language | eng |
recordid | cdi_proquest_miscellaneous_2862201984 |
source | Elsevier ScienceDirect Journals Complete |
subjects | Diabetes Insulin mutation Metabolite Mouse Plasma amino acid profile |
title | A specific plasma amino acid profile in the Insulin2Q104del Kuma mice at the diabetic state and reversal from hyperglycemia |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T00%3A42%3A08IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_elsev&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20specific%20plasma%20amino%20acid%20profile%20in%20the%20Insulin2Q104del%20Kuma%20mice%20at%20the%20diabetic%20state%20and%20reversal%20from%20hyperglycemia&rft.jtitle=Biochemical%20and%20biophysical%20research%20communications&rft.au=Hiyoshi,%20Naoya&rft.date=2023-10-30&rft.volume=679&rft.spage=58&rft.epage=65&rft.pages=58-65&rft.issn=0006-291X&rft.eissn=1090-2104&rft_id=info:doi/10.1016/j.bbrc.2023.08.064&rft_dat=%3Cproquest_elsev%3E2862201984%3C/proquest_elsev%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2862201984&rft_id=info:pmid/&rft_els_id=S0006291X23010264&rfr_iscdi=true |