Metabolic Resistance of Sogatella furcifera (Hemiptera: Delphacidae) toward Pymetrozine Involves the Overexpression of CYP6FJ3
Sogatella furcifera (Horváth), which mainly threatens rice, shows various levels of pesticide resistance due to long-term overuse of pesticides. Our resistance monitoring of 20 field populations in Sichuan, China, revealed that they were susceptible to highly resistant toward pymetrozine (0.4–142.2...
Gespeichert in:
| Veröffentlicht in: | Journal of agricultural and food chemistry 2023-10, Vol.71 (39), p.14179-14191 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 14191 |
|---|---|
| container_issue | 39 |
| container_start_page | 14179 |
| container_title | Journal of agricultural and food chemistry |
| container_volume | 71 |
| creator | Gong, Changwei Liu, Dan Wang, Qiulin Ma, Yanxin Zhan, Xiaoxu Zhang, Shuirong Awais, Muhammad Pu, Jian Yang, Jizhi Wang, Xuegui |
| description | Sogatella furcifera (Horváth), which mainly threatens rice, shows various levels of pesticide resistance due to long-term overuse of pesticides. Our resistance monitoring of 20 field populations in Sichuan, China, revealed that they were susceptible to highly resistant toward pymetrozine (0.4–142.2 RR), and JL21 reached the highest level of resistance. The JL21 population exhibited cross-resistance to triflumezopyrim and dinotefuran but sensitivity to sulfoxaflor, acetamiprid, clothianidin, and nitenpyram. The increased P450 activity were support to involve in pymetrozine resistance by detoxification enzyme activities and synergist determination. Among 16 candidate P450 genes, CYP6FJ3 (5.25-fold) was the most up-regulated in JL21, while no significant change was found after LC25 pymetrozine treatment. Furthermore, the knockdown by RNAi and heterologous overexpression by the GAL4/UAS system confirmed that the CYP6FJ3 overexpression was involved in the pymetrozine resistance, and recombination in vitro confirmed that CYP6FJ3 could hydroxylate pymetrozine. Therefore, the overexpression of CYP6FJ3 promotes pymetrozine metabolic resistance in S. furcifera. |
| doi_str_mv | 10.1021/acs.jafc.3c03617 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2860617106</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2860617106</sourcerecordid><originalsourceid>FETCH-LOGICAL-a266t-9e1ab49ccd6ceba02b0fa97bcec7db1fd0cb95dd0a1b7d33e6ac8065867e5ed93</originalsourceid><addsrcrecordid>eNp1kElPwzAQRi0EEmW5c_QRJFLGCXESbqhQFhUVsRw4RRN7AkZpHGy3LAd-OynlymlGmvd90jzG9gQMBcTiCJUfvmKthomCRIpsjQ1EGkOUCpGvswH0TJSnUmyyLe9fASBPMxiw7xsKWNnGKH5H3viArSJua35vnzFQ0yCv506Zmhzy_UuamS706wk_o6Z7QWU00gEP9h2d5refMwrOfpmW-FW7sM2CPA8vxKcLcvTROfLe2HZZP3q6lePrZIdt1Nh42v2b2-xxfP4wuowm04ur0ekkwljKEBUksDoulNJSUYUQV1BjkVWKVKYrUWtQVZFqDSiqTCcJSVQ5yDSXGaWki2Sb7a96O2ff5uRDOTNeLd9ryc59GecSemkCZI_CClXOeu-oLjtnZug-SwHlUnXZqy6Xqss_1X3kcBX5vdi5a_tf_sd_AIVzhWg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2860617106</pqid></control><display><type>article</type><title>Metabolic Resistance of Sogatella furcifera (Hemiptera: Delphacidae) toward Pymetrozine Involves the Overexpression of CYP6FJ3</title><source>American Chemical Society (ACS) Journals</source><creator>Gong, Changwei ; Liu, Dan ; Wang, Qiulin ; Ma, Yanxin ; Zhan, Xiaoxu ; Zhang, Shuirong ; Awais, Muhammad ; Pu, Jian ; Yang, Jizhi ; Wang, Xuegui</creator><creatorcontrib>Gong, Changwei ; Liu, Dan ; Wang, Qiulin ; Ma, Yanxin ; Zhan, Xiaoxu ; Zhang, Shuirong ; Awais, Muhammad ; Pu, Jian ; Yang, Jizhi ; Wang, Xuegui</creatorcontrib><description>Sogatella furcifera (Horváth), which mainly threatens rice, shows various levels of pesticide resistance due to long-term overuse of pesticides. Our resistance monitoring of 20 field populations in Sichuan, China, revealed that they were susceptible to highly resistant toward pymetrozine (0.4–142.2 RR), and JL21 reached the highest level of resistance. The JL21 population exhibited cross-resistance to triflumezopyrim and dinotefuran but sensitivity to sulfoxaflor, acetamiprid, clothianidin, and nitenpyram. The increased P450 activity were support to involve in pymetrozine resistance by detoxification enzyme activities and synergist determination. Among 16 candidate P450 genes, CYP6FJ3 (5.25-fold) was the most up-regulated in JL21, while no significant change was found after LC25 pymetrozine treatment. Furthermore, the knockdown by RNAi and heterologous overexpression by the GAL4/UAS system confirmed that the CYP6FJ3 overexpression was involved in the pymetrozine resistance, and recombination in vitro confirmed that CYP6FJ3 could hydroxylate pymetrozine. Therefore, the overexpression of CYP6FJ3 promotes pymetrozine metabolic resistance in S. furcifera.</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/acs.jafc.3c03617</identifier><language>eng</language><publisher>American Chemical Society</publisher><subject>Agricultural and Environmental Chemistry</subject><ispartof>Journal of agricultural and food chemistry, 2023-10, Vol.71 (39), p.14179-14191</ispartof><rights>2023 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-a266t-9e1ab49ccd6ceba02b0fa97bcec7db1fd0cb95dd0a1b7d33e6ac8065867e5ed93</cites><orcidid>0000-0002-5218-8530</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.jafc.3c03617$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.jafc.3c03617$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids></links><search><creatorcontrib>Gong, Changwei</creatorcontrib><creatorcontrib>Liu, Dan</creatorcontrib><creatorcontrib>Wang, Qiulin</creatorcontrib><creatorcontrib>Ma, Yanxin</creatorcontrib><creatorcontrib>Zhan, Xiaoxu</creatorcontrib><creatorcontrib>Zhang, Shuirong</creatorcontrib><creatorcontrib>Awais, Muhammad</creatorcontrib><creatorcontrib>Pu, Jian</creatorcontrib><creatorcontrib>Yang, Jizhi</creatorcontrib><creatorcontrib>Wang, Xuegui</creatorcontrib><title>Metabolic Resistance of Sogatella furcifera (Hemiptera: Delphacidae) toward Pymetrozine Involves the Overexpression of CYP6FJ3</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>Sogatella furcifera (Horváth), which mainly threatens rice, shows various levels of pesticide resistance due to long-term overuse of pesticides. Our resistance monitoring of 20 field populations in Sichuan, China, revealed that they were susceptible to highly resistant toward pymetrozine (0.4–142.2 RR), and JL21 reached the highest level of resistance. The JL21 population exhibited cross-resistance to triflumezopyrim and dinotefuran but sensitivity to sulfoxaflor, acetamiprid, clothianidin, and nitenpyram. The increased P450 activity were support to involve in pymetrozine resistance by detoxification enzyme activities and synergist determination. Among 16 candidate P450 genes, CYP6FJ3 (5.25-fold) was the most up-regulated in JL21, while no significant change was found after LC25 pymetrozine treatment. Furthermore, the knockdown by RNAi and heterologous overexpression by the GAL4/UAS system confirmed that the CYP6FJ3 overexpression was involved in the pymetrozine resistance, and recombination in vitro confirmed that CYP6FJ3 could hydroxylate pymetrozine. Therefore, the overexpression of CYP6FJ3 promotes pymetrozine metabolic resistance in S. furcifera.</description><subject>Agricultural and Environmental Chemistry</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNp1kElPwzAQRi0EEmW5c_QRJFLGCXESbqhQFhUVsRw4RRN7AkZpHGy3LAd-OynlymlGmvd90jzG9gQMBcTiCJUfvmKthomCRIpsjQ1EGkOUCpGvswH0TJSnUmyyLe9fASBPMxiw7xsKWNnGKH5H3viArSJua35vnzFQ0yCv506Zmhzy_UuamS706wk_o6Z7QWU00gEP9h2d5refMwrOfpmW-FW7sM2CPA8vxKcLcvTROfLe2HZZP3q6lePrZIdt1Nh42v2b2-xxfP4wuowm04ur0ekkwljKEBUksDoulNJSUYUQV1BjkVWKVKYrUWtQVZFqDSiqTCcJSVQ5yDSXGaWki2Sb7a96O2ff5uRDOTNeLd9ryc59GecSemkCZI_CClXOeu-oLjtnZug-SwHlUnXZqy6Xqss_1X3kcBX5vdi5a_tf_sd_AIVzhWg</recordid><startdate>20231004</startdate><enddate>20231004</enddate><creator>Gong, Changwei</creator><creator>Liu, Dan</creator><creator>Wang, Qiulin</creator><creator>Ma, Yanxin</creator><creator>Zhan, Xiaoxu</creator><creator>Zhang, Shuirong</creator><creator>Awais, Muhammad</creator><creator>Pu, Jian</creator><creator>Yang, Jizhi</creator><creator>Wang, Xuegui</creator><general>American Chemical Society</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-5218-8530</orcidid></search><sort><creationdate>20231004</creationdate><title>Metabolic Resistance of Sogatella furcifera (Hemiptera: Delphacidae) toward Pymetrozine Involves the Overexpression of CYP6FJ3</title><author>Gong, Changwei ; Liu, Dan ; Wang, Qiulin ; Ma, Yanxin ; Zhan, Xiaoxu ; Zhang, Shuirong ; Awais, Muhammad ; Pu, Jian ; Yang, Jizhi ; Wang, Xuegui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a266t-9e1ab49ccd6ceba02b0fa97bcec7db1fd0cb95dd0a1b7d33e6ac8065867e5ed93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Agricultural and Environmental Chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gong, Changwei</creatorcontrib><creatorcontrib>Liu, Dan</creatorcontrib><creatorcontrib>Wang, Qiulin</creatorcontrib><creatorcontrib>Ma, Yanxin</creatorcontrib><creatorcontrib>Zhan, Xiaoxu</creatorcontrib><creatorcontrib>Zhang, Shuirong</creatorcontrib><creatorcontrib>Awais, Muhammad</creatorcontrib><creatorcontrib>Pu, Jian</creatorcontrib><creatorcontrib>Yang, Jizhi</creatorcontrib><creatorcontrib>Wang, Xuegui</creatorcontrib><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gong, Changwei</au><au>Liu, Dan</au><au>Wang, Qiulin</au><au>Ma, Yanxin</au><au>Zhan, Xiaoxu</au><au>Zhang, Shuirong</au><au>Awais, Muhammad</au><au>Pu, Jian</au><au>Yang, Jizhi</au><au>Wang, Xuegui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Metabolic Resistance of Sogatella furcifera (Hemiptera: Delphacidae) toward Pymetrozine Involves the Overexpression of CYP6FJ3</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2023-10-04</date><risdate>2023</risdate><volume>71</volume><issue>39</issue><spage>14179</spage><epage>14191</epage><pages>14179-14191</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><abstract>Sogatella furcifera (Horváth), which mainly threatens rice, shows various levels of pesticide resistance due to long-term overuse of pesticides. Our resistance monitoring of 20 field populations in Sichuan, China, revealed that they were susceptible to highly resistant toward pymetrozine (0.4–142.2 RR), and JL21 reached the highest level of resistance. The JL21 population exhibited cross-resistance to triflumezopyrim and dinotefuran but sensitivity to sulfoxaflor, acetamiprid, clothianidin, and nitenpyram. The increased P450 activity were support to involve in pymetrozine resistance by detoxification enzyme activities and synergist determination. Among 16 candidate P450 genes, CYP6FJ3 (5.25-fold) was the most up-regulated in JL21, while no significant change was found after LC25 pymetrozine treatment. Furthermore, the knockdown by RNAi and heterologous overexpression by the GAL4/UAS system confirmed that the CYP6FJ3 overexpression was involved in the pymetrozine resistance, and recombination in vitro confirmed that CYP6FJ3 could hydroxylate pymetrozine. Therefore, the overexpression of CYP6FJ3 promotes pymetrozine metabolic resistance in S. furcifera.</abstract><pub>American Chemical Society</pub><doi>10.1021/acs.jafc.3c03617</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-5218-8530</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-8561 |
| ispartof | Journal of agricultural and food chemistry, 2023-10, Vol.71 (39), p.14179-14191 |
| issn | 0021-8561 1520-5118 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2860617106 |
| source | American Chemical Society (ACS) Journals |
| subjects | Agricultural and Environmental Chemistry |
| title | Metabolic Resistance of Sogatella furcifera (Hemiptera: Delphacidae) toward Pymetrozine Involves the Overexpression of CYP6FJ3 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-01T01%3A31%3A19IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Metabolic%20Resistance%20of%20Sogatella%20furcifera%20(Hemiptera:%20Delphacidae)%20toward%20Pymetrozine%20Involves%20the%20Overexpression%20of%20CYP6FJ3&rft.jtitle=Journal%20of%20agricultural%20and%20food%20chemistry&rft.au=Gong,%20Changwei&rft.date=2023-10-04&rft.volume=71&rft.issue=39&rft.spage=14179&rft.epage=14191&rft.pages=14179-14191&rft.issn=0021-8561&rft.eissn=1520-5118&rft_id=info:doi/10.1021/acs.jafc.3c03617&rft_dat=%3Cproquest_cross%3E2860617106%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2860617106&rft_id=info:pmid/&rfr_iscdi=true |