Smart design of universally decorated nanoparticles for drug delivery applications driven by active transport
Targeting the cell nucleus remains a challenge for drug delivery. Here, we present a universal platform for the smart design of nanoparticle (NP) decoration that is based on: (i) a spacer polymer, commonly biotin-polyethylene-glycol-thiol, whose grafting density and molecular weight can be tuned for...
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| Veröffentlicht in: | The European physical journal. E, Soft matter and biological physics Soft matter and biological physics, 2023-09, Vol.46 (9), p.74-74, Article 74 |
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| container_title | The European physical journal. E, Soft matter and biological physics |
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| creator | Halbi, Gal Fayer, Itay Aranovich, Dina Gat, Shachar Pavan, Mariela J. Nachmias, Dikla Sanchez, Daniel Sevilla Brik, Ashraf Granek, Rony Bernheim-Groswasser, Anne |
| description | Targeting the cell nucleus remains a challenge for drug delivery. Here, we present a universal platform for the smart design of nanoparticle (NP) decoration that is based on: (i) a spacer polymer, commonly biotin-polyethylene-glycol-thiol, whose grafting density and molecular weight can be tuned for optimized performance, and (ii) protein binding peptides, such as cell penetrating peptides (CPPs), cancer-targeting peptides, or nuclear localization signal (NLS) peptides, that are linked to the PEG free-end by universal chemistry. We manifested our platform with two different bromo-acetamide (Br-Ac) modified NLSs. We used cell extract-based and live cell assays to demonstrate the recruitment of dynein motor proteins, which drive the NP active transport toward the nucleus, and the enhancement of cellular and nuclear entry, manifesting the properties of NLS as a CPP. Our control of the NP decoration scheme, and the modularity of our platform, carry great advantages for nano-carrier design for drug delivery applications.
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| doi_str_mv | 10.1140/epje/s10189-023-00331-5 |
| format | Article |
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E, Soft matter and biological physics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Halbi, Gal</au><au>Fayer, Itay</au><au>Aranovich, Dina</au><au>Gat, Shachar</au><au>Pavan, Mariela J.</au><au>Nachmias, Dikla</au><au>Sanchez, Daniel Sevilla</au><au>Brik, Ashraf</au><au>Granek, Rony</au><au>Bernheim-Groswasser, Anne</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Smart design of universally decorated nanoparticles for drug delivery applications driven by active transport</atitle><jtitle>The European physical journal. E, Soft matter and biological physics</jtitle><stitle>Eur. Phys. J. E</stitle><date>2023-09-01</date><risdate>2023</risdate><volume>46</volume><issue>9</issue><spage>74</spage><epage>74</epage><pages>74-74</pages><artnum>74</artnum><issn>1292-8941</issn><eissn>1292-895X</eissn><abstract>Targeting the cell nucleus remains a challenge for drug delivery. Here, we present a universal platform for the smart design of nanoparticle (NP) decoration that is based on: (i) a spacer polymer, commonly biotin-polyethylene-glycol-thiol, whose grafting density and molecular weight can be tuned for optimized performance, and (ii) protein binding peptides, such as cell penetrating peptides (CPPs), cancer-targeting peptides, or nuclear localization signal (NLS) peptides, that are linked to the PEG free-end by universal chemistry. We manifested our platform with two different bromo-acetamide (Br-Ac) modified NLSs. We used cell extract-based and live cell assays to demonstrate the recruitment of dynein motor proteins, which drive the NP active transport toward the nucleus, and the enhancement of cellular and nuclear entry, manifesting the properties of NLS as a CPP. Our control of the NP decoration scheme, and the modularity of our platform, carry great advantages for nano-carrier design for drug delivery applications.
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| subjects | Biological and Medical Physics Biophysics Biotin Complex Fluids and Microfluidics Complex Systems Condensed matter physics Decoration Drug carriers Festschrift in honor of Philip (Fyl) Pincus Modularity Molecular motors Nanoparticles Nanotechnology Nuclei (cytology) Peptides Physics Physics and Astronomy Polymer Sciences Proteins Regular Article - Living Systems Soft and Granular Matter Surfaces and Interfaces Thin Films |
| title | Smart design of universally decorated nanoparticles for drug delivery applications driven by active transport |
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