Porous silicon as an entrapping matrix for the immobilization of urease

Porous silicon as an entrapping matrix for the immobilization of urease

Electrochemically anodized surface of p-type silicon (porous silicon) is reported to be an efficient entrapping template for immobilization of urease. The porosity and the amount of loading of the enzyme are optimized for obtaining highest efficiency. The effect of entrapment and therefore the activ...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Sensors and actuators. B, Chemical Chemical, 2005-05, Vol.107 (1), p.258-263
Hauptverfasser: Chaudhari, Prajakta S., Gokarna, Anisha, Kulkarni, Manjusha, Karve, M.S., Bhoraskar, S.V.
Format: Artikel
Sprache:eng
Schlagworte:
Immobilization
Porous silicon
Urea
Urease
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 263
container_issue 1
container_start_page 258
container_title Sensors and actuators. B, Chemical
container_volume 107
creator Chaudhari, Prajakta S.
Gokarna, Anisha
Kulkarni, Manjusha
Karve, M.S.
Bhoraskar, S.V.
description Electrochemically anodized surface of p-type silicon (porous silicon) is reported to be an efficient entrapping template for immobilization of urease. The porosity and the amount of loading of the enzyme are optimized for obtaining highest efficiency. The effect of entrapment and therefore the activity retained by the porous silicon surface is monitored by colorimetric assay. The yield in photoluminescence of porous silicon at 610 nm is seen to get affected by the presence of enzyme. The change in the surface morphology has been studied by scanning electron microscopy and it is observed that the immobilized system has successfully retained urease enzyme. The immobilized system gives linear response for concentrations of urea ranging in values between 3.3 × 10 −5 and 2.4 × 10 −4 M Porous silicon is seen to be capable of holding the urease enzyme for repeated cyclic tests for five times. The system exhibited a shelf life of 15 days.
doi_str_mv 10.1016/j.snb.2004.10.009
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_28584275</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0925400504007038</els_id><sourcerecordid>28584275</sourcerecordid><originalsourceid>FETCH-LOGICAL-c328t-a1f92e2a2d3d168d99b2f87d69aadb9287c27d396e33de9d5e6ec3722c40491e3</originalsourceid><addsrcrecordid>eNp9kE1LxDAQhoMouK7-AG85eWvNR9s0eJJFV2FBD3oOaTLVLG1Tk6yov94s69nTMMPzDrwPQpeUlJTQ5npbxqkrGSFV3ktC5BFa0FbwghMhjtGCSFYXFSH1KTqLcUsyyBuyQOtnH_wu4ugGZ_yEdcR6wjCloOfZTW941Cm4L9z7gNM7YDeOvsvsj04u477HuwA6wjk66fUQ4eJvLtHr_d3L6qHYPK0fV7ebwnDWpkLTXjJgmlluadNaKTvWt8I2UmvbSdYKw4TlsgHOLUhbQwOGC8ZMRSpJgS_R1eHvHPzHDmJSo4sGhkFPkHso1tZtxUSdQXoATfAxBujVHNyow7eiRO2Vqa3KytRe2f6UleXMzSEDucGng6CicTAZsC6AScp690_6F5mOdLQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>28584275</pqid></control><display><type>article</type><title>Porous silicon as an entrapping matrix for the immobilization of urease</title><source>Elsevier ScienceDirect Journals Complete</source><creator>Chaudhari, Prajakta S. ; Gokarna, Anisha ; Kulkarni, Manjusha ; Karve, M.S. ; Bhoraskar, S.V.</creator><creatorcontrib>Chaudhari, Prajakta S. ; Gokarna, Anisha ; Kulkarni, Manjusha ; Karve, M.S. ; Bhoraskar, S.V.</creatorcontrib><description>Electrochemically anodized surface of p-type silicon (porous silicon) is reported to be an efficient entrapping template for immobilization of urease. The porosity and the amount of loading of the enzyme are optimized for obtaining highest efficiency. The effect of entrapment and therefore the activity retained by the porous silicon surface is monitored by colorimetric assay. The yield in photoluminescence of porous silicon at 610 nm is seen to get affected by the presence of enzyme. The change in the surface morphology has been studied by scanning electron microscopy and it is observed that the immobilized system has successfully retained urease enzyme. The immobilized system gives linear response for concentrations of urea ranging in values between 3.3 × 10 −5 and 2.4 × 10 −4 M Porous silicon is seen to be capable of holding the urease enzyme for repeated cyclic tests for five times. The system exhibited a shelf life of 15 days.</description><identifier>ISSN: 0925-4005</identifier><identifier>EISSN: 1873-3077</identifier><identifier>DOI: 10.1016/j.snb.2004.10.009</identifier><language>eng</language><publisher>Elsevier B.V</publisher><subject>Immobilization ; Porous silicon ; Urea ; Urease</subject><ispartof>Sensors and actuators. B, Chemical, 2005-05, Vol.107 (1), p.258-263</ispartof><rights>2004 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c328t-a1f92e2a2d3d168d99b2f87d69aadb9287c27d396e33de9d5e6ec3722c40491e3</citedby><cites>FETCH-LOGICAL-c328t-a1f92e2a2d3d168d99b2f87d69aadb9287c27d396e33de9d5e6ec3722c40491e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.snb.2004.10.009$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids></links><search><creatorcontrib>Chaudhari, Prajakta S.</creatorcontrib><creatorcontrib>Gokarna, Anisha</creatorcontrib><creatorcontrib>Kulkarni, Manjusha</creatorcontrib><creatorcontrib>Karve, M.S.</creatorcontrib><creatorcontrib>Bhoraskar, S.V.</creatorcontrib><title>Porous silicon as an entrapping matrix for the immobilization of urease</title><title>Sensors and actuators. B, Chemical</title><description>Electrochemically anodized surface of p-type silicon (porous silicon) is reported to be an efficient entrapping template for immobilization of urease. The porosity and the amount of loading of the enzyme are optimized for obtaining highest efficiency. The effect of entrapment and therefore the activity retained by the porous silicon surface is monitored by colorimetric assay. The yield in photoluminescence of porous silicon at 610 nm is seen to get affected by the presence of enzyme. The change in the surface morphology has been studied by scanning electron microscopy and it is observed that the immobilized system has successfully retained urease enzyme. The immobilized system gives linear response for concentrations of urea ranging in values between 3.3 × 10 −5 and 2.4 × 10 −4 M Porous silicon is seen to be capable of holding the urease enzyme for repeated cyclic tests for five times. The system exhibited a shelf life of 15 days.</description><subject>Immobilization</subject><subject>Porous silicon</subject><subject>Urea</subject><subject>Urease</subject><issn>0925-4005</issn><issn>1873-3077</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNp9kE1LxDAQhoMouK7-AG85eWvNR9s0eJJFV2FBD3oOaTLVLG1Tk6yov94s69nTMMPzDrwPQpeUlJTQ5npbxqkrGSFV3ktC5BFa0FbwghMhjtGCSFYXFSH1KTqLcUsyyBuyQOtnH_wu4ugGZ_yEdcR6wjCloOfZTW941Cm4L9z7gNM7YDeOvsvsj04u477HuwA6wjk66fUQ4eJvLtHr_d3L6qHYPK0fV7ebwnDWpkLTXjJgmlluadNaKTvWt8I2UmvbSdYKw4TlsgHOLUhbQwOGC8ZMRSpJgS_R1eHvHPzHDmJSo4sGhkFPkHso1tZtxUSdQXoATfAxBujVHNyow7eiRO2Vqa3KytRe2f6UleXMzSEDucGng6CicTAZsC6AScp690_6F5mOdLQ</recordid><startdate>20050527</startdate><enddate>20050527</enddate><creator>Chaudhari, Prajakta S.</creator><creator>Gokarna, Anisha</creator><creator>Kulkarni, Manjusha</creator><creator>Karve, M.S.</creator><creator>Bhoraskar, S.V.</creator><general>Elsevier B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope></search><sort><creationdate>20050527</creationdate><title>Porous silicon as an entrapping matrix for the immobilization of urease</title><author>Chaudhari, Prajakta S. ; Gokarna, Anisha ; Kulkarni, Manjusha ; Karve, M.S. ; Bhoraskar, S.V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c328t-a1f92e2a2d3d168d99b2f87d69aadb9287c27d396e33de9d5e6ec3722c40491e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Immobilization</topic><topic>Porous silicon</topic><topic>Urea</topic><topic>Urease</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chaudhari, Prajakta S.</creatorcontrib><creatorcontrib>Gokarna, Anisha</creatorcontrib><creatorcontrib>Kulkarni, Manjusha</creatorcontrib><creatorcontrib>Karve, M.S.</creatorcontrib><creatorcontrib>Bhoraskar, S.V.</creatorcontrib><collection>CrossRef</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Sensors and actuators. B, Chemical</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chaudhari, Prajakta S.</au><au>Gokarna, Anisha</au><au>Kulkarni, Manjusha</au><au>Karve, M.S.</au><au>Bhoraskar, S.V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Porous silicon as an entrapping matrix for the immobilization of urease</atitle><jtitle>Sensors and actuators. B, Chemical</jtitle><date>2005-05-27</date><risdate>2005</risdate><volume>107</volume><issue>1</issue><spage>258</spage><epage>263</epage><pages>258-263</pages><issn>0925-4005</issn><eissn>1873-3077</eissn><abstract>Electrochemically anodized surface of p-type silicon (porous silicon) is reported to be an efficient entrapping template for immobilization of urease. The porosity and the amount of loading of the enzyme are optimized for obtaining highest efficiency. The effect of entrapment and therefore the activity retained by the porous silicon surface is monitored by colorimetric assay. The yield in photoluminescence of porous silicon at 610 nm is seen to get affected by the presence of enzyme. The change in the surface morphology has been studied by scanning electron microscopy and it is observed that the immobilized system has successfully retained urease enzyme. The immobilized system gives linear response for concentrations of urea ranging in values between 3.3 × 10 −5 and 2.4 × 10 −4 M Porous silicon is seen to be capable of holding the urease enzyme for repeated cyclic tests for five times. The system exhibited a shelf life of 15 days.</abstract><pub>Elsevier B.V</pub><doi>10.1016/j.snb.2004.10.009</doi><tpages>6</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0925-4005
ispartof Sensors and actuators. B, Chemical, 2005-05, Vol.107 (1), p.258-263
issn 0925-4005
1873-3077
language eng
recordid cdi_proquest_miscellaneous_28584275
source Elsevier ScienceDirect Journals Complete
subjects Immobilization
Porous silicon
Urea
Urease
title Porous silicon as an entrapping matrix for the immobilization of urease
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-14T15%3A31%3A10IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Porous%20silicon%20as%20an%20entrapping%20matrix%20for%20the%20immobilization%20of%20urease&rft.jtitle=Sensors%20and%20actuators.%20B,%20Chemical&rft.au=Chaudhari,%20Prajakta%20S.&rft.date=2005-05-27&rft.volume=107&rft.issue=1&rft.spage=258&rft.epage=263&rft.pages=258-263&rft.issn=0925-4005&rft.eissn=1873-3077&rft_id=info:doi/10.1016/j.snb.2004.10.009&rft_dat=%3Cproquest_cross%3E28584275%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=28584275&rft_id=info:pmid/&rft_els_id=S0925400504007038&rfr_iscdi=true