Transcriptomic investigation of the molecular mechanisms underlying resistance to the neonicotinoid thiamethoxam and the pyrethroid lambda‐cyhalothrin in Euschistus heros (Hemiptera: Pentatomidae)
BACKGROUND Laboratory‐selected resistant strains of Euschistus heros to thiamethoxam (NEO) and lambda‐cyhalothrin (PYR) were recently reported in Brazil. However, the mechanisms conferring resistance to these insecticides in E. heros remain unresolved. We utilized comparative transcriptome profiling...
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description | BACKGROUND
Laboratory‐selected resistant strains of Euschistus heros to thiamethoxam (NEO) and lambda‐cyhalothrin (PYR) were recently reported in Brazil. However, the mechanisms conferring resistance to these insecticides in E. heros remain unresolved. We utilized comparative transcriptome profiling and single nucleotide polymorphism (SNP) calling of susceptible and resistant strains of E. heros to investigate the molecular mechanism(s) underlying resistance.
RESULTS
The E. heros transcriptome was assembled, generating 91 673 transcripts with a mean length of 720 bp and N50 of 1795 bp. Comparative gene expression analysis between the susceptible (SUS) and NEO strains identified 215 significantly differentially expressed (DE) transcripts. DE transcripts associated with the xenobiotic metabolism were all up‐regulated in the NEO strain. The comparative analysis of the SUS and PYR strains identified 204 DE transcripts, including an esterase (esterase FE4), a glutathione‐S‐transferase, an ABC transporter (ABCC1) and aquaporins that were up‐regulated in the PYR strain. We identified 9588 and 15 043 nonsynonymous SNPs in the PYR and NEO strains. One of the SNPs (D70N) detected in the NEO strain occurs in a subunit (α5) of the nAChRs, the target site of neonicotinoid insecticides. Nevertheless, this residue position in α5 is not conserved among insects.
CONCLUSIONS
Neonicotinoid and pyrethroid resistance in laboratory‐selected E. heros is associated with a potential metabolic resistance mechanism by the overexpression of proteins commonly involved in the three phases of xenobiotic metabolism. Together these findings provide insight into the potential basis of resistance in E. heros and will inform the development and implementation of resistance management strategies against this important pest. © 2023 Society of Chemical Industry.
Transcriptome and SNP‐calling analyses revealed the main mechanism underlying resistance of E. heros to thiamethoxam and lambda‐cyhalothrin is metabolic resistance. Two other potential mechanisms are highlighted: the cuticular penetration resistance in the λ‐cyhalothrin‐resistant strain, and the target‐site mutation in the thiamethoxam‐resistant strain of E. heros. |
doi_str_mv | 10.1002/ps.7745 |
format | Article |
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Laboratory‐selected resistant strains of Euschistus heros to thiamethoxam (NEO) and lambda‐cyhalothrin (PYR) were recently reported in Brazil. However, the mechanisms conferring resistance to these insecticides in E. heros remain unresolved. We utilized comparative transcriptome profiling and single nucleotide polymorphism (SNP) calling of susceptible and resistant strains of E. heros to investigate the molecular mechanism(s) underlying resistance.
RESULTS
The E. heros transcriptome was assembled, generating 91 673 transcripts with a mean length of 720 bp and N50 of 1795 bp. Comparative gene expression analysis between the susceptible (SUS) and NEO strains identified 215 significantly differentially expressed (DE) transcripts. DE transcripts associated with the xenobiotic metabolism were all up‐regulated in the NEO strain. The comparative analysis of the SUS and PYR strains identified 204 DE transcripts, including an esterase (esterase FE4), a glutathione‐S‐transferase, an ABC transporter (ABCC1) and aquaporins that were up‐regulated in the PYR strain. We identified 9588 and 15 043 nonsynonymous SNPs in the PYR and NEO strains. One of the SNPs (D70N) detected in the NEO strain occurs in a subunit (α5) of the nAChRs, the target site of neonicotinoid insecticides. Nevertheless, this residue position in α5 is not conserved among insects.
CONCLUSIONS
Neonicotinoid and pyrethroid resistance in laboratory‐selected E. heros is associated with a potential metabolic resistance mechanism by the overexpression of proteins commonly involved in the three phases of xenobiotic metabolism. Together these findings provide insight into the potential basis of resistance in E. heros and will inform the development and implementation of resistance management strategies against this important pest. © 2023 Society of Chemical Industry.
Transcriptome and SNP‐calling analyses revealed the main mechanism underlying resistance of E. heros to thiamethoxam and lambda‐cyhalothrin is metabolic resistance. Two other potential mechanisms are highlighted: the cuticular penetration resistance in the λ‐cyhalothrin‐resistant strain, and the target‐site mutation in the thiamethoxam‐resistant strain of E. heros.</description><identifier>ISSN: 1526-498X</identifier><identifier>EISSN: 1526-4998</identifier><identifier>DOI: 10.1002/ps.7745</identifier><identifier>PMID: 37624650</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>ABC transporter ; Acetylcholine receptors (nicotinic) ; Aquaporins ; Comparative analysis ; Cyhalothrin ; de novo transcriptome ; differential gene expression ; Esterase ; Euschistus heros ; Gene expression ; Glutathione ; Insecticide resistance ; insecticide resistance mechanisms ; Insecticides ; Insects ; Metabolism ; Molecular modelling ; Neotropical brown stink bug ; Nucleotides ; Pest resistance ; pesticide resistance ; Polymorphism ; Pyrethroids ; Single-nucleotide polymorphism ; SNP calling ; Strain analysis ; Thiamethoxam ; Transcriptomes ; Transcriptomics</subject><ispartof>Pest management science, 2023-12, Vol.79 (12), p.5349-5361</ispartof><rights>2023 Society of Chemical Industry.</rights><rights>Copyright © 2023 Society of Chemical Industry</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3455-2ef4e4f01a4676eed326526f2fcd3099e08e6d0911af315c0e239cd6ac6c9e573</citedby><cites>FETCH-LOGICAL-c3455-2ef4e4f01a4676eed326526f2fcd3099e08e6d0911af315c0e239cd6ac6c9e573</cites><orcidid>0000-0002-2590-1492 ; 0000-0001-9391-6509 ; 0000-0002-4929-9088 ; 0000-0002-2287-0782 ; 0000-0002-6432-830X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fps.7745$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fps.7745$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,27926,27927,45576,45577</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37624650$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lira, Ewerton C</creatorcontrib><creatorcontrib>Nascimento, Antonio RB</creatorcontrib><creatorcontrib>Bass, Chris</creatorcontrib><creatorcontrib>Omoto, Celso</creatorcontrib><creatorcontrib>Cônsoli, Fernando L</creatorcontrib><title>Transcriptomic investigation of the molecular mechanisms underlying resistance to the neonicotinoid thiamethoxam and the pyrethroid lambda‐cyhalothrin in Euschistus heros (Hemiptera: Pentatomidae)</title><title>Pest management science</title><addtitle>Pest Manag Sci</addtitle><description>BACKGROUND
Laboratory‐selected resistant strains of Euschistus heros to thiamethoxam (NEO) and lambda‐cyhalothrin (PYR) were recently reported in Brazil. However, the mechanisms conferring resistance to these insecticides in E. heros remain unresolved. We utilized comparative transcriptome profiling and single nucleotide polymorphism (SNP) calling of susceptible and resistant strains of E. heros to investigate the molecular mechanism(s) underlying resistance.
RESULTS
The E. heros transcriptome was assembled, generating 91 673 transcripts with a mean length of 720 bp and N50 of 1795 bp. Comparative gene expression analysis between the susceptible (SUS) and NEO strains identified 215 significantly differentially expressed (DE) transcripts. DE transcripts associated with the xenobiotic metabolism were all up‐regulated in the NEO strain. The comparative analysis of the SUS and PYR strains identified 204 DE transcripts, including an esterase (esterase FE4), a glutathione‐S‐transferase, an ABC transporter (ABCC1) and aquaporins that were up‐regulated in the PYR strain. We identified 9588 and 15 043 nonsynonymous SNPs in the PYR and NEO strains. One of the SNPs (D70N) detected in the NEO strain occurs in a subunit (α5) of the nAChRs, the target site of neonicotinoid insecticides. Nevertheless, this residue position in α5 is not conserved among insects.
CONCLUSIONS
Neonicotinoid and pyrethroid resistance in laboratory‐selected E. heros is associated with a potential metabolic resistance mechanism by the overexpression of proteins commonly involved in the three phases of xenobiotic metabolism. Together these findings provide insight into the potential basis of resistance in E. heros and will inform the development and implementation of resistance management strategies against this important pest. © 2023 Society of Chemical Industry.
Transcriptome and SNP‐calling analyses revealed the main mechanism underlying resistance of E. heros to thiamethoxam and lambda‐cyhalothrin is metabolic resistance. Two other potential mechanisms are highlighted: the cuticular penetration resistance in the λ‐cyhalothrin‐resistant strain, and the target‐site mutation in the thiamethoxam‐resistant strain of E. heros.</description><subject>ABC transporter</subject><subject>Acetylcholine receptors (nicotinic)</subject><subject>Aquaporins</subject><subject>Comparative analysis</subject><subject>Cyhalothrin</subject><subject>de novo transcriptome</subject><subject>differential gene expression</subject><subject>Esterase</subject><subject>Euschistus heros</subject><subject>Gene expression</subject><subject>Glutathione</subject><subject>Insecticide resistance</subject><subject>insecticide resistance mechanisms</subject><subject>Insecticides</subject><subject>Insects</subject><subject>Metabolism</subject><subject>Molecular modelling</subject><subject>Neotropical brown stink bug</subject><subject>Nucleotides</subject><subject>Pest resistance</subject><subject>pesticide resistance</subject><subject>Polymorphism</subject><subject>Pyrethroids</subject><subject>Single-nucleotide polymorphism</subject><subject>SNP calling</subject><subject>Strain analysis</subject><subject>Thiamethoxam</subject><subject>Transcriptomes</subject><subject>Transcriptomics</subject><issn>1526-498X</issn><issn>1526-4998</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNp1kd1qFTEUhQdRbK3iG0jACytyaiYzyUy8k1KtUGjBCt4Nu8meTkp-psmMOnc-gk_lg_RJmnNO2wvBq4SdL4u11yqKlyU9KCll78d00DQ1f1TslpyJVS1l-_jh3n7fKZ6ldEUplVKyp8VO1QhWC053i7_nEXxS0YxTcEYR439gmswlTCZ4EnoyDUhcsKhmC5E4VAN4k1wis9cY7WL8JYmYTJrAKyRT2PzwGLxRYTI-GJ0nBhxOQ_gFjoDXG2RcYh7F9bsFd6Hh5vcftQxgQ54an52QozmpISvPiQwYQyL7x-iyU4zwgZyhn2BtWgO-fV486cEmfHF37hXfPh2dHx6vTk4_fzn8eLJSVc35imFfY93TEmrRCERdMZEz6lmvdJXDQdqi0FSWJfRVyRVFVkmlBSihJPKm2iv2t7pjDNdzTqpzJim0FvLGc-pYy5uWU0F5Rl__g16FOfrsLlOtlCVt6jpTb7aUyvuliH03RuMgLl1Ju3W13Zi6dbWZfHWnN1841A_cfZcZeLcFfhqLy_90urOvG7lb4GKzUA</recordid><startdate>202312</startdate><enddate>202312</enddate><creator>Lira, Ewerton C</creator><creator>Nascimento, Antonio RB</creator><creator>Bass, Chris</creator><creator>Omoto, Celso</creator><creator>Cônsoli, Fernando L</creator><general>John Wiley & Sons, Ltd</general><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>SOI</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-2590-1492</orcidid><orcidid>https://orcid.org/0000-0001-9391-6509</orcidid><orcidid>https://orcid.org/0000-0002-4929-9088</orcidid><orcidid>https://orcid.org/0000-0002-2287-0782</orcidid><orcidid>https://orcid.org/0000-0002-6432-830X</orcidid></search><sort><creationdate>202312</creationdate><title>Transcriptomic investigation of the molecular mechanisms underlying resistance to the neonicotinoid thiamethoxam and the pyrethroid lambda‐cyhalothrin in Euschistus heros (Hemiptera: Pentatomidae)</title><author>Lira, Ewerton C ; Nascimento, Antonio RB ; Bass, Chris ; Omoto, Celso ; Cônsoli, Fernando L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3455-2ef4e4f01a4676eed326526f2fcd3099e08e6d0911af315c0e239cd6ac6c9e573</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>ABC transporter</topic><topic>Acetylcholine receptors (nicotinic)</topic><topic>Aquaporins</topic><topic>Comparative analysis</topic><topic>Cyhalothrin</topic><topic>de novo transcriptome</topic><topic>differential gene expression</topic><topic>Esterase</topic><topic>Euschistus heros</topic><topic>Gene expression</topic><topic>Glutathione</topic><topic>Insecticide resistance</topic><topic>insecticide resistance mechanisms</topic><topic>Insecticides</topic><topic>Insects</topic><topic>Metabolism</topic><topic>Molecular modelling</topic><topic>Neotropical brown stink bug</topic><topic>Nucleotides</topic><topic>Pest resistance</topic><topic>pesticide resistance</topic><topic>Polymorphism</topic><topic>Pyrethroids</topic><topic>Single-nucleotide polymorphism</topic><topic>SNP calling</topic><topic>Strain analysis</topic><topic>Thiamethoxam</topic><topic>Transcriptomes</topic><topic>Transcriptomics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lira, Ewerton C</creatorcontrib><creatorcontrib>Nascimento, Antonio RB</creatorcontrib><creatorcontrib>Bass, Chris</creatorcontrib><creatorcontrib>Omoto, Celso</creatorcontrib><creatorcontrib>Cônsoli, Fernando L</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Pest management science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lira, Ewerton C</au><au>Nascimento, Antonio RB</au><au>Bass, Chris</au><au>Omoto, Celso</au><au>Cônsoli, Fernando L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transcriptomic investigation of the molecular mechanisms underlying resistance to the neonicotinoid thiamethoxam and the pyrethroid lambda‐cyhalothrin in Euschistus heros (Hemiptera: Pentatomidae)</atitle><jtitle>Pest management science</jtitle><addtitle>Pest Manag Sci</addtitle><date>2023-12</date><risdate>2023</risdate><volume>79</volume><issue>12</issue><spage>5349</spage><epage>5361</epage><pages>5349-5361</pages><issn>1526-498X</issn><eissn>1526-4998</eissn><abstract>BACKGROUND
Laboratory‐selected resistant strains of Euschistus heros to thiamethoxam (NEO) and lambda‐cyhalothrin (PYR) were recently reported in Brazil. However, the mechanisms conferring resistance to these insecticides in E. heros remain unresolved. We utilized comparative transcriptome profiling and single nucleotide polymorphism (SNP) calling of susceptible and resistant strains of E. heros to investigate the molecular mechanism(s) underlying resistance.
RESULTS
The E. heros transcriptome was assembled, generating 91 673 transcripts with a mean length of 720 bp and N50 of 1795 bp. Comparative gene expression analysis between the susceptible (SUS) and NEO strains identified 215 significantly differentially expressed (DE) transcripts. DE transcripts associated with the xenobiotic metabolism were all up‐regulated in the NEO strain. The comparative analysis of the SUS and PYR strains identified 204 DE transcripts, including an esterase (esterase FE4), a glutathione‐S‐transferase, an ABC transporter (ABCC1) and aquaporins that were up‐regulated in the PYR strain. We identified 9588 and 15 043 nonsynonymous SNPs in the PYR and NEO strains. One of the SNPs (D70N) detected in the NEO strain occurs in a subunit (α5) of the nAChRs, the target site of neonicotinoid insecticides. Nevertheless, this residue position in α5 is not conserved among insects.
CONCLUSIONS
Neonicotinoid and pyrethroid resistance in laboratory‐selected E. heros is associated with a potential metabolic resistance mechanism by the overexpression of proteins commonly involved in the three phases of xenobiotic metabolism. Together these findings provide insight into the potential basis of resistance in E. heros and will inform the development and implementation of resistance management strategies against this important pest. © 2023 Society of Chemical Industry.
Transcriptome and SNP‐calling analyses revealed the main mechanism underlying resistance of E. heros to thiamethoxam and lambda‐cyhalothrin is metabolic resistance. Two other potential mechanisms are highlighted: the cuticular penetration resistance in the λ‐cyhalothrin‐resistant strain, and the target‐site mutation in the thiamethoxam‐resistant strain of E. heros.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>37624650</pmid><doi>10.1002/ps.7745</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-2590-1492</orcidid><orcidid>https://orcid.org/0000-0001-9391-6509</orcidid><orcidid>https://orcid.org/0000-0002-4929-9088</orcidid><orcidid>https://orcid.org/0000-0002-2287-0782</orcidid><orcidid>https://orcid.org/0000-0002-6432-830X</orcidid></addata></record> |
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subjects | ABC transporter Acetylcholine receptors (nicotinic) Aquaporins Comparative analysis Cyhalothrin de novo transcriptome differential gene expression Esterase Euschistus heros Gene expression Glutathione Insecticide resistance insecticide resistance mechanisms Insecticides Insects Metabolism Molecular modelling Neotropical brown stink bug Nucleotides Pest resistance pesticide resistance Polymorphism Pyrethroids Single-nucleotide polymorphism SNP calling Strain analysis Thiamethoxam Transcriptomes Transcriptomics |
title | Transcriptomic investigation of the molecular mechanisms underlying resistance to the neonicotinoid thiamethoxam and the pyrethroid lambda‐cyhalothrin in Euschistus heros (Hemiptera: Pentatomidae) |
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