Micellar electrokinetic capillary chromatography of methylxanthines-containing beverages: discussion of the molecular species involved
Micellar electrokinetic capillary chromatography (MECC) experimental conditions were applied to 12 samples of methylxanthine-containing infusions of different commercial brands of yerba mate, coffee, tea and cocoa as well as two cola drinks. The best resolution in this mode of automated high-perform...
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| Veröffentlicht in: | Journal of the science of food and agriculture 2005-03, Vol.85 (4), p.622-628 |
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| creator | Pomilio, A.B Trajtemberg, S.P Vitale, A.A |
| description | Micellar electrokinetic capillary chromatography (MECC) experimental conditions were applied to 12 samples of methylxanthine-containing infusions of different commercial brands of yerba mate, coffee, tea and cocoa as well as two cola drinks. The best resolution in this mode of automated high-performance capillary electrophoresis (HPCE) was achieved here when using 15 kV voltage in an uncoated fused-silica capillary of 45 cm length (40 cm effective length), 50 mM sodium dodecylsulfate, 90 mM pH 8.5 borate buffer and UV detection. Theobromine, caffeine and theophylline were separated, and the peak splitting due to tautomeric species was observed. Experimental conditions were controlled, keeping constant the size of the elution window in each analysis. The limit of detection was less than 1 mg l(-1), the limit of quantitation was 2.5 mg l(-1) and the work range was 2.5-300 mg l(-1). This HPCE-MECC system has proved suitable for the analysis/quality control of xanthines in beverages for consumption. Roles of various parameters as well as distinctly charged species of each xanthine and the origin of peak splitting in this MECC system are discussed. |
| doi_str_mv | 10.1002/jsfa.2020 |
| format | Article |
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The best resolution in this mode of automated high-performance capillary electrophoresis (HPCE) was achieved here when using 15 kV voltage in an uncoated fused-silica capillary of 45 cm length (40 cm effective length), 50 mM sodium dodecylsulfate, 90 mM pH 8.5 borate buffer and UV detection. Theobromine, caffeine and theophylline were separated, and the peak splitting due to tautomeric species was observed. Experimental conditions were controlled, keeping constant the size of the elution window in each analysis. The limit of detection was less than 1 mg l(-1), the limit of quantitation was 2.5 mg l(-1) and the work range was 2.5-300 mg l(-1). This HPCE-MECC system has proved suitable for the analysis/quality control of xanthines in beverages for consumption. Roles of various parameters as well as distinctly charged species of each xanthine and the origin of peak splitting in this MECC system are discussed.</description><identifier>ISSN: 0022-5142</identifier><identifier>EISSN: 1097-0010</identifier><identifier>DOI: 10.1002/jsfa.2020</identifier><identifier>CODEN: JSFAAE</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>automated high-performance capillary electrophoresis (HPCE) ; Beverages ; Biological and medical sciences ; caffeine ; Chemical compounds ; Chromatography ; cocoa (beverage) ; coffee (beverage) ; cola drinks ; food analysis ; food composition ; Food industries ; Fundamental and applied biological sciences. Psychology ; keto-enol tautomerism ; micellar electrokinetic capillary chromatography ; micellar electrokinetic capillary chromatography (MECC) ; quinine ; Research methodology ; soft drinks ; tea ; theobromine ; theophylline ; yerba mate</subject><ispartof>Journal of the science of food and agriculture, 2005-03, Vol.85 (4), p.622-628</ispartof><rights>Copyright © 2004 Society of Chemical Industry</rights><rights>2005 INIST-CNRS</rights><rights>Copyright John Wiley and Sons, Limited Mar 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4190-ab31bfbb6ca8af8a1e51f3e46513b4f369e4da5de8210f4d926e14b362bfff153</citedby><cites>FETCH-LOGICAL-c4190-ab31bfbb6ca8af8a1e51f3e46513b4f369e4da5de8210f4d926e14b362bfff153</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjsfa.2020$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjsfa.2020$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16517919$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Pomilio, A.B</creatorcontrib><creatorcontrib>Trajtemberg, S.P</creatorcontrib><creatorcontrib>Vitale, A.A</creatorcontrib><title>Micellar electrokinetic capillary chromatography of methylxanthines-containing beverages: discussion of the molecular species involved</title><title>Journal of the science of food and agriculture</title><addtitle>J. Sci. Food Agric</addtitle><description>Micellar electrokinetic capillary chromatography (MECC) experimental conditions were applied to 12 samples of methylxanthine-containing infusions of different commercial brands of yerba mate, coffee, tea and cocoa as well as two cola drinks. The best resolution in this mode of automated high-performance capillary electrophoresis (HPCE) was achieved here when using 15 kV voltage in an uncoated fused-silica capillary of 45 cm length (40 cm effective length), 50 mM sodium dodecylsulfate, 90 mM pH 8.5 borate buffer and UV detection. Theobromine, caffeine and theophylline were separated, and the peak splitting due to tautomeric species was observed. Experimental conditions were controlled, keeping constant the size of the elution window in each analysis. The limit of detection was less than 1 mg l(-1), the limit of quantitation was 2.5 mg l(-1) and the work range was 2.5-300 mg l(-1). This HPCE-MECC system has proved suitable for the analysis/quality control of xanthines in beverages for consumption. Roles of various parameters as well as distinctly charged species of each xanthine and the origin of peak splitting in this MECC system are discussed.</description><subject>automated high-performance capillary electrophoresis (HPCE)</subject><subject>Beverages</subject><subject>Biological and medical sciences</subject><subject>caffeine</subject><subject>Chemical compounds</subject><subject>Chromatography</subject><subject>cocoa (beverage)</subject><subject>coffee (beverage)</subject><subject>cola drinks</subject><subject>food analysis</subject><subject>food composition</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>keto-enol tautomerism</subject><subject>micellar electrokinetic capillary chromatography</subject><subject>micellar electrokinetic capillary chromatography (MECC)</subject><subject>quinine</subject><subject>Research methodology</subject><subject>soft drinks</subject><subject>tea</subject><subject>theobromine</subject><subject>theophylline</subject><subject>yerba mate</subject><issn>0022-5142</issn><issn>1097-0010</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNp1ks1u1DAUhSMEEkNhwRMQIVGpi7S24zhjdtWUlp8BFkMFO8txrieeJnawk2nnBXhuHGUEEhIrS_Z3zj33XifJS4zOMULkYhe0PCeIoEfJAiNeZghh9DhZxDeSFZiSp8mzEHYIIc4ZWyS_PhsFbSt9Ci2owbs7Y2EwKlWyN9P9IVWNd50c3NbLvjmkTqcdDM2hfZB2aCIdMuXsII01dptWsAcvtxDeprUJagzBODtphgbSzsUa41Qs9KAMhNTYvWv3UD9PnmjZBnhxPE-S2-t331bvs_XXmw-ry3WmKOYok1WOK11VTMml1EuJocA6B8oKnFdU54wDrWVRw5JgpGnNCQNMq5yRSmuNi_wkOZ19e-9-jhAG0cWU0wAsuDEIsiwwYyWL4Ot_wJ0bvY3ZBCGkxAXlJEJnM6S8C8GDFr03XZyZwEhM6xDTOsS0jsi-ORrKoGSrvbTKhL-C2ELJMY_cxczdmxYO_zcUHzfXl0fnbFaYMMDDH4X0d4KVeVmI719uBOerH5tPV2txFflXM6-lE3LrY4rbDUE4j3-ipJzT_DegvrX5</recordid><startdate>200503</startdate><enddate>200503</enddate><creator>Pomilio, A.B</creator><creator>Trajtemberg, S.P</creator><creator>Vitale, A.A</creator><general>John Wiley & Sons, Ltd</general><general>Wiley</general><general>John Wiley and Sons, Limited</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QL</scope><scope>7QQ</scope><scope>7QR</scope><scope>7SC</scope><scope>7SE</scope><scope>7SN</scope><scope>7SP</scope><scope>7SR</scope><scope>7ST</scope><scope>7T5</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>M7N</scope><scope>P64</scope><scope>SOI</scope></search><sort><creationdate>200503</creationdate><title>Micellar electrokinetic capillary chromatography of methylxanthines-containing beverages: discussion of the molecular species involved</title><author>Pomilio, A.B ; Trajtemberg, S.P ; Vitale, A.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4190-ab31bfbb6ca8af8a1e51f3e46513b4f369e4da5de8210f4d926e14b362bfff153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>automated high-performance capillary electrophoresis (HPCE)</topic><topic>Beverages</topic><topic>Biological and medical sciences</topic><topic>caffeine</topic><topic>Chemical compounds</topic><topic>Chromatography</topic><topic>cocoa (beverage)</topic><topic>coffee (beverage)</topic><topic>cola drinks</topic><topic>food analysis</topic><topic>food composition</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>keto-enol tautomerism</topic><topic>micellar electrokinetic capillary chromatography</topic><topic>micellar electrokinetic capillary chromatography (MECC)</topic><topic>quinine</topic><topic>Research methodology</topic><topic>soft drinks</topic><topic>tea</topic><topic>theobromine</topic><topic>theophylline</topic><topic>yerba mate</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pomilio, A.B</creatorcontrib><creatorcontrib>Trajtemberg, S.P</creatorcontrib><creatorcontrib>Vitale, A.A</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Ceramic Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Ecology Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Environment Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><jtitle>Journal of the science of food and agriculture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pomilio, A.B</au><au>Trajtemberg, S.P</au><au>Vitale, A.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Micellar electrokinetic capillary chromatography of methylxanthines-containing beverages: discussion of the molecular species involved</atitle><jtitle>Journal of the science of food and agriculture</jtitle><addtitle>J. Sci. Food Agric</addtitle><date>2005-03</date><risdate>2005</risdate><volume>85</volume><issue>4</issue><spage>622</spage><epage>628</epage><pages>622-628</pages><issn>0022-5142</issn><eissn>1097-0010</eissn><coden>JSFAAE</coden><abstract>Micellar electrokinetic capillary chromatography (MECC) experimental conditions were applied to 12 samples of methylxanthine-containing infusions of different commercial brands of yerba mate, coffee, tea and cocoa as well as two cola drinks. The best resolution in this mode of automated high-performance capillary electrophoresis (HPCE) was achieved here when using 15 kV voltage in an uncoated fused-silica capillary of 45 cm length (40 cm effective length), 50 mM sodium dodecylsulfate, 90 mM pH 8.5 borate buffer and UV detection. Theobromine, caffeine and theophylline were separated, and the peak splitting due to tautomeric species was observed. Experimental conditions were controlled, keeping constant the size of the elution window in each analysis. The limit of detection was less than 1 mg l(-1), the limit of quantitation was 2.5 mg l(-1) and the work range was 2.5-300 mg l(-1). This HPCE-MECC system has proved suitable for the analysis/quality control of xanthines in beverages for consumption. Roles of various parameters as well as distinctly charged species of each xanthine and the origin of peak splitting in this MECC system are discussed.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><doi>10.1002/jsfa.2020</doi><tpages>7</tpages></addata></record> |
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| ispartof | Journal of the science of food and agriculture, 2005-03, Vol.85 (4), p.622-628 |
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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | automated high-performance capillary electrophoresis (HPCE) Beverages Biological and medical sciences caffeine Chemical compounds Chromatography cocoa (beverage) coffee (beverage) cola drinks food analysis food composition Food industries Fundamental and applied biological sciences. Psychology keto-enol tautomerism micellar electrokinetic capillary chromatography micellar electrokinetic capillary chromatography (MECC) quinine Research methodology soft drinks tea theobromine theophylline yerba mate |
| title | Micellar electrokinetic capillary chromatography of methylxanthines-containing beverages: discussion of the molecular species involved |
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