Alginate coated biogenic silver nanoparticles for the treatment of Pseudomonas infections in rainbow trout
Pseudomonas species are among the main pathogens causing rainbow trout infections. The present study provides a simple, green, sustainable, and rapid technique to synthesize of biogenic alginate-capped silver nanoparticles (Alg-Ag NPs) suitable for the treatment of Pseudomonas infections. It has bee...
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| Veröffentlicht in: | International journal of biological macromolecules 2023-11, Vol.251, p.126302, Article 126302 |
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| creator | Kulikouskaya, Viktoryia Nikalaichuk, Viktoryia Hileuskaya, Kseniya Ladutska, Alena Grigoryan, Karine Kozerozhets, Irina Hovsepyan, Varduhi Sargsyan, Mariam Sidarenka, Anastasiya |
| description | Pseudomonas species are among the main pathogens causing rainbow trout infections. The present study provides a simple, green, sustainable, and rapid technique to synthesize of biogenic alginate-capped silver nanoparticles (Alg-Ag NPs) suitable for the treatment of Pseudomonas infections. It has been shown that the mechanism (aggregative or autocatalytic) of Alg-Ag NPs formation depended on Alg concentration and the heating approach used. The rate constants and activation energy were calculated. Alg-Ag NPs were characterized by UV-Vis, FTIR, XRD, TEM, AFM, XPS, and DLS. The optimal conditions for the fabrication of spherically-shaped (17-19 nm) and negatively-charged (zeta-potential |
| doi_str_mv | 10.1016/j.ijbiomac.2023.126302 |
| format | Article |
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The present study provides a simple, green, sustainable, and rapid technique to synthesize of biogenic alginate-capped silver nanoparticles (Alg-Ag NPs) suitable for the treatment of Pseudomonas infections. It has been shown that the mechanism (aggregative or autocatalytic) of Alg-Ag NPs formation depended on Alg concentration and the heating approach used. The rate constants and activation energy were calculated. Alg-Ag NPs were characterized by UV-Vis, FTIR, XRD, TEM, AFM, XPS, and DLS. The optimal conditions for the fabrication of spherically-shaped (17-19 nm) and negatively-charged (zeta-potential <-50 mV) Alg-Ag NPs, which are stable during 9 months, included hot-plate assisted synthesis at 100 °C in diluted (1 mg/mL) Alg solutions. In vitro studies showed that Alg-Ag NPs exhibited prominent antimicrobial activity against collection Pseudomonas strains (inhibition zones ranged from 9.0 ± 1.0 to 19.0 ± 1.0 mm), with no significant loss of antibacterial efficacy after 9 months of storage. AFM analysis confirmed that the antibacterial effect of Alg-Ag NPs dealt with the direct nanomechanical disrupting of bacterial cells. The ability of Alg-Ag NPs to inhibit the growth of virulent P.aeruginosa, P.fluorescens and P. putida strains isolated from infected rainbow trout was evaluated. All tested strains were susceptible to Alg(10)-Ag NPs, while Alg(1)-Ag NPs demonstrated a limited strain-specific antibacterial effect. The obtained data displayed the prospects for the application of biogenic Alg-Ag NPs to create novel delivery systems for combating Pseudomonas infections in rainbow trout.</description><identifier>ISSN: 0141-8130</identifier><identifier>ISSN: 1879-0003</identifier><identifier>EISSN: 1879-0003</identifier><identifier>DOI: 10.1016/j.ijbiomac.2023.126302</identifier><identifier>PMID: 37573909</identifier><language>eng</language><publisher>Netherlands</publisher><subject>activation energy ; alginates ; Alginates - chemistry ; Alginates - pharmacology ; Animals ; Anti-Bacterial Agents - chemistry ; Anti-Bacterial Agents - pharmacology ; antibacterial properties ; Fish Diseases - drug therapy ; Fish Diseases - microbiology ; Metal Nanoparticles - chemistry ; Microbial Sensitivity Tests ; nanosilver ; Oncorhynchus mykiss ; Oncorhynchus mykiss - microbiology ; Pseudomonas ; Pseudomonas - drug effects ; Pseudomonas Infections - drug therapy ; Pseudomonas Infections - microbiology ; rapid methods ; Silver - chemistry ; Silver - pharmacology ; species ; virulence ; zeta potential</subject><ispartof>International journal of biological macromolecules, 2023-11, Vol.251, p.126302, Article 126302</ispartof><rights>Copyright © 2023 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c344t-4b92af5c8baf4b91034af02469fb9ecbc2c4a348c9f5c089e7ec6cabea0430dd3</citedby><cites>FETCH-LOGICAL-c344t-4b92af5c8baf4b91034af02469fb9ecbc2c4a348c9f5c089e7ec6cabea0430dd3</cites><orcidid>0000-0001-6505-3929</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37573909$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kulikouskaya, Viktoryia</creatorcontrib><creatorcontrib>Nikalaichuk, Viktoryia</creatorcontrib><creatorcontrib>Hileuskaya, Kseniya</creatorcontrib><creatorcontrib>Ladutska, Alena</creatorcontrib><creatorcontrib>Grigoryan, Karine</creatorcontrib><creatorcontrib>Kozerozhets, Irina</creatorcontrib><creatorcontrib>Hovsepyan, Varduhi</creatorcontrib><creatorcontrib>Sargsyan, Mariam</creatorcontrib><creatorcontrib>Sidarenka, Anastasiya</creatorcontrib><title>Alginate coated biogenic silver nanoparticles for the treatment of Pseudomonas infections in rainbow trout</title><title>International journal of biological macromolecules</title><addtitle>Int J Biol Macromol</addtitle><description>Pseudomonas species are among the main pathogens causing rainbow trout infections. The present study provides a simple, green, sustainable, and rapid technique to synthesize of biogenic alginate-capped silver nanoparticles (Alg-Ag NPs) suitable for the treatment of Pseudomonas infections. It has been shown that the mechanism (aggregative or autocatalytic) of Alg-Ag NPs formation depended on Alg concentration and the heating approach used. The rate constants and activation energy were calculated. Alg-Ag NPs were characterized by UV-Vis, FTIR, XRD, TEM, AFM, XPS, and DLS. The optimal conditions for the fabrication of spherically-shaped (17-19 nm) and negatively-charged (zeta-potential <-50 mV) Alg-Ag NPs, which are stable during 9 months, included hot-plate assisted synthesis at 100 °C in diluted (1 mg/mL) Alg solutions. In vitro studies showed that Alg-Ag NPs exhibited prominent antimicrobial activity against collection Pseudomonas strains (inhibition zones ranged from 9.0 ± 1.0 to 19.0 ± 1.0 mm), with no significant loss of antibacterial efficacy after 9 months of storage. AFM analysis confirmed that the antibacterial effect of Alg-Ag NPs dealt with the direct nanomechanical disrupting of bacterial cells. The ability of Alg-Ag NPs to inhibit the growth of virulent P.aeruginosa, P.fluorescens and P. putida strains isolated from infected rainbow trout was evaluated. All tested strains were susceptible to Alg(10)-Ag NPs, while Alg(1)-Ag NPs demonstrated a limited strain-specific antibacterial effect. The obtained data displayed the prospects for the application of biogenic Alg-Ag NPs to create novel delivery systems for combating Pseudomonas infections in rainbow trout.</description><subject>activation energy</subject><subject>alginates</subject><subject>Alginates - chemistry</subject><subject>Alginates - pharmacology</subject><subject>Animals</subject><subject>Anti-Bacterial Agents - chemistry</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>antibacterial properties</subject><subject>Fish Diseases - drug therapy</subject><subject>Fish Diseases - microbiology</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Microbial Sensitivity Tests</subject><subject>nanosilver</subject><subject>Oncorhynchus mykiss</subject><subject>Oncorhynchus mykiss - microbiology</subject><subject>Pseudomonas</subject><subject>Pseudomonas - drug effects</subject><subject>Pseudomonas Infections - drug therapy</subject><subject>Pseudomonas Infections - microbiology</subject><subject>rapid methods</subject><subject>Silver - chemistry</subject><subject>Silver - pharmacology</subject><subject>species</subject><subject>virulence</subject><subject>zeta potential</subject><issn>0141-8130</issn><issn>1879-0003</issn><issn>1879-0003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1P3DAQhq0KVJaPv4B87CXL-CNfR7SCFmklOMDZcpwxdZTYW9sp6r8nq93tlcvMaPS8M4eHkFsGawasuhvWbuhcmLRZc-BizXglgH8jK9bUbQEA4oysgElWNEzABblMaVi2Vcma7-RC1GUtWmhXZLgf353XGakJS-3pcvQdvTM0ufEvRuq1DzsdszMjJmpDpPk30hxR5wl9psHSl4RzH6bgdaLOWzTZBb8fadTOd-FjwcOcr8m51WPCm2O_Im-PD6-bX8X2-efT5n5bGCFlLmTXcm1L03TaLjMDIbUFLqvWdi2aznAjtZCNaRcImhZrNJXRHWqQAvpeXJEfh7u7GP7MmLKaXDI4jtpjmJMSrJSsZHXFv0R5U0LNyrpmC1odUBNDShGt2kU36fhPMVB7JWpQJyVqr0QdlCzB2-OPuZuw_x87ORCfMlqM7Q</recordid><startdate>20231101</startdate><enddate>20231101</enddate><creator>Kulikouskaya, Viktoryia</creator><creator>Nikalaichuk, Viktoryia</creator><creator>Hileuskaya, Kseniya</creator><creator>Ladutska, Alena</creator><creator>Grigoryan, Karine</creator><creator>Kozerozhets, Irina</creator><creator>Hovsepyan, Varduhi</creator><creator>Sargsyan, Mariam</creator><creator>Sidarenka, Anastasiya</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0001-6505-3929</orcidid></search><sort><creationdate>20231101</creationdate><title>Alginate coated biogenic silver nanoparticles for the treatment of Pseudomonas infections in rainbow trout</title><author>Kulikouskaya, Viktoryia ; Nikalaichuk, Viktoryia ; Hileuskaya, Kseniya ; Ladutska, Alena ; Grigoryan, Karine ; Kozerozhets, Irina ; Hovsepyan, Varduhi ; Sargsyan, Mariam ; Sidarenka, Anastasiya</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c344t-4b92af5c8baf4b91034af02469fb9ecbc2c4a348c9f5c089e7ec6cabea0430dd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>activation energy</topic><topic>alginates</topic><topic>Alginates - chemistry</topic><topic>Alginates - pharmacology</topic><topic>Animals</topic><topic>Anti-Bacterial Agents - chemistry</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>antibacterial properties</topic><topic>Fish Diseases - drug therapy</topic><topic>Fish Diseases - microbiology</topic><topic>Metal Nanoparticles - chemistry</topic><topic>Microbial Sensitivity Tests</topic><topic>nanosilver</topic><topic>Oncorhynchus mykiss</topic><topic>Oncorhynchus mykiss - microbiology</topic><topic>Pseudomonas</topic><topic>Pseudomonas - drug effects</topic><topic>Pseudomonas Infections - drug therapy</topic><topic>Pseudomonas Infections - microbiology</topic><topic>rapid methods</topic><topic>Silver - chemistry</topic><topic>Silver - pharmacology</topic><topic>species</topic><topic>virulence</topic><topic>zeta potential</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kulikouskaya, Viktoryia</creatorcontrib><creatorcontrib>Nikalaichuk, Viktoryia</creatorcontrib><creatorcontrib>Hileuskaya, Kseniya</creatorcontrib><creatorcontrib>Ladutska, Alena</creatorcontrib><creatorcontrib>Grigoryan, Karine</creatorcontrib><creatorcontrib>Kozerozhets, Irina</creatorcontrib><creatorcontrib>Hovsepyan, Varduhi</creatorcontrib><creatorcontrib>Sargsyan, Mariam</creatorcontrib><creatorcontrib>Sidarenka, Anastasiya</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>International journal of biological macromolecules</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kulikouskaya, Viktoryia</au><au>Nikalaichuk, Viktoryia</au><au>Hileuskaya, Kseniya</au><au>Ladutska, Alena</au><au>Grigoryan, Karine</au><au>Kozerozhets, Irina</au><au>Hovsepyan, Varduhi</au><au>Sargsyan, Mariam</au><au>Sidarenka, Anastasiya</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Alginate coated biogenic silver nanoparticles for the treatment of Pseudomonas infections in rainbow trout</atitle><jtitle>International journal of biological macromolecules</jtitle><addtitle>Int J Biol Macromol</addtitle><date>2023-11-01</date><risdate>2023</risdate><volume>251</volume><spage>126302</spage><pages>126302-</pages><artnum>126302</artnum><issn>0141-8130</issn><issn>1879-0003</issn><eissn>1879-0003</eissn><abstract>Pseudomonas species are among the main pathogens causing rainbow trout infections. The present study provides a simple, green, sustainable, and rapid technique to synthesize of biogenic alginate-capped silver nanoparticles (Alg-Ag NPs) suitable for the treatment of Pseudomonas infections. It has been shown that the mechanism (aggregative or autocatalytic) of Alg-Ag NPs formation depended on Alg concentration and the heating approach used. The rate constants and activation energy were calculated. Alg-Ag NPs were characterized by UV-Vis, FTIR, XRD, TEM, AFM, XPS, and DLS. The optimal conditions for the fabrication of spherically-shaped (17-19 nm) and negatively-charged (zeta-potential <-50 mV) Alg-Ag NPs, which are stable during 9 months, included hot-plate assisted synthesis at 100 °C in diluted (1 mg/mL) Alg solutions. In vitro studies showed that Alg-Ag NPs exhibited prominent antimicrobial activity against collection Pseudomonas strains (inhibition zones ranged from 9.0 ± 1.0 to 19.0 ± 1.0 mm), with no significant loss of antibacterial efficacy after 9 months of storage. AFM analysis confirmed that the antibacterial effect of Alg-Ag NPs dealt with the direct nanomechanical disrupting of bacterial cells. The ability of Alg-Ag NPs to inhibit the growth of virulent P.aeruginosa, P.fluorescens and P. putida strains isolated from infected rainbow trout was evaluated. All tested strains were susceptible to Alg(10)-Ag NPs, while Alg(1)-Ag NPs demonstrated a limited strain-specific antibacterial effect. The obtained data displayed the prospects for the application of biogenic Alg-Ag NPs to create novel delivery systems for combating Pseudomonas infections in rainbow trout.</abstract><cop>Netherlands</cop><pmid>37573909</pmid><doi>10.1016/j.ijbiomac.2023.126302</doi><orcidid>https://orcid.org/0000-0001-6505-3929</orcidid></addata></record> |
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| subjects | activation energy alginates Alginates - chemistry Alginates - pharmacology Animals Anti-Bacterial Agents - chemistry Anti-Bacterial Agents - pharmacology antibacterial properties Fish Diseases - drug therapy Fish Diseases - microbiology Metal Nanoparticles - chemistry Microbial Sensitivity Tests nanosilver Oncorhynchus mykiss Oncorhynchus mykiss - microbiology Pseudomonas Pseudomonas - drug effects Pseudomonas Infections - drug therapy Pseudomonas Infections - microbiology rapid methods Silver - chemistry Silver - pharmacology species virulence zeta potential |
| title | Alginate coated biogenic silver nanoparticles for the treatment of Pseudomonas infections in rainbow trout |
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