Structure-Guided Engineering of a Protease to Improve Its Activity under Cold Conditions

proteases commonly exhibit remarkably reduced activity under cold conditions. Herein, we employed a tailored combination of a loop engineering strategy and iterative saturation mutagenesis method to engineer two loops for substrate binding at the entrance of the substrate tunnel of a protease (bcPRO...

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Veröffentlicht in:	Journal of agricultural and food chemistry 2023-08, Vol.71 (33), p.12528-12537
Hauptverfasser:	Wang, Fenghua, Ma, Xiangyang, Sun, Ying, Guo, Enping, Shi, Chaoshuo, Yuan, Zhaoting, Li, Yu, Li, Qinggang, Lu, Fuping, Liu, Yihan
Format:	Artikel
Sprache:	eng
Online-Zugang:	Volltext
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