Quantification of skin penetration of caffeine and propylene glycol applied topically in a mixture by tailored multivariate curve resolution‐alternating least squares of depth‐resolved Raman spectra
The quantitative determination of topically applied substances in the skin is severely limited and represents a challenging task. The porcine skin ex vivo was topically treated with a gel containing caffeine (CF) and propylene glycol (PG), and depth‐resolved Raman spectra were recorded with two conf...
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| Veröffentlicht in: | Journal of biophotonics 2023-12, Vol.16 (12), p.e202300146-n/a |
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| creator | Choe, ChunSik Pak, Gyong Jin Ascencio, Saul Mujica Darvin, Maxim E. |
| description | The quantitative determination of topically applied substances in the skin is severely limited and represents a challenging task. The porcine skin ex vivo was topically treated with a gel containing caffeine (CF) and propylene glycol (PG), and depth‐resolved Raman spectra were recorded with two confocal Raman microscopes. We applied a novel tailored multivariate curve resolution‐alternating least squares method to the selected spectral regions (512–604 and 778–1148 cm−1) of gel‐treated skin and quantitatively determined the concentrations of CF and PG in the stratum corneum (SC). The highest concentration of CF (181 mg/cm3) was found at the surface, while PG (384 mg/cm3) was found at 10% SC depth, indicating the formation of a reservoir at the superficial SC. The concentrations of CF and PG decreased monotonically and reached the detection limit at ≈60% and ≈80% SC depth, respectively, indicating that neither permeate the SC.
The novel tailored multivariate curve resolution‐alternating least squares method is used to quantitatively determine the penetration profiles of caffeine (CF) and propylene glycol (PG) applied to the skin in a mixture. PG creates a reservoir at the superficial stratum corneum (SC) depth. The concentrations of CF and PG decrease monotonically and reach the detection limit at a depth of 60% SC depth (≈9 μm) and 80% SC depth (≈12 μm), respectively, indicating that neither permeate the SC. |
| doi_str_mv | 10.1002/jbio.202300146 |
| format | Article |
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The novel tailored multivariate curve resolution‐alternating least squares method is used to quantitatively determine the penetration profiles of caffeine (CF) and propylene glycol (PG) applied to the skin in a mixture. PG creates a reservoir at the superficial stratum corneum (SC) depth. The concentrations of CF and PG decrease monotonically and reach the detection limit at a depth of 60% SC depth (≈9 μm) and 80% SC depth (≈12 μm), respectively, indicating that neither permeate the SC.</description><identifier>ISSN: 1864-063X</identifier><identifier>EISSN: 1864-0648</identifier><identifier>DOI: 10.1002/jbio.202300146</identifier><identifier>PMID: 37556739</identifier><language>eng</language><publisher>Weinheim: WILEY‐VCH Verlag GmbH & Co. KGaA</publisher><subject>Animals ; anti‐aging ; Caffeine ; cosmetics ; depth profiles ; drug delivery ; Epidermis ; keratin ; Least squares method ; Least-Squares Analysis ; Microscopes ; Multivariate analysis ; penetration pathways ; Propylene ; Propylene Glycol ; Raman micro‐spectroscopy ; Raman spectra ; Raman spectroscopy ; Skin ; skin barrier function ; spectra normalization ; Spectrum Analysis, Raman - methods ; Stratum corneum ; Swine ; transdermal delivery</subject><ispartof>Journal of biophotonics, 2023-12, Vol.16 (12), p.e202300146-n/a</ispartof><rights>2023 Wiley‐VCH GmbH.</rights><rights>2023 Wiley-VCH GmbH.</rights><rights>2023 Wiley‐VCH GmbH</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3736-76c730279d2efe42b0e10eddca7fa72f5296bf7aefe49b7068a6638aef8977e3</citedby><cites>FETCH-LOGICAL-c3736-76c730279d2efe42b0e10eddca7fa72f5296bf7aefe49b7068a6638aef8977e3</cites><orcidid>0000-0003-1075-1994</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjbio.202300146$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjbio.202300146$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37556739$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Choe, ChunSik</creatorcontrib><creatorcontrib>Pak, Gyong Jin</creatorcontrib><creatorcontrib>Ascencio, Saul Mujica</creatorcontrib><creatorcontrib>Darvin, Maxim E.</creatorcontrib><title>Quantification of skin penetration of caffeine and propylene glycol applied topically in a mixture by tailored multivariate curve resolution‐alternating least squares of depth‐resolved Raman spectra</title><title>Journal of biophotonics</title><addtitle>J Biophotonics</addtitle><description>The quantitative determination of topically applied substances in the skin is severely limited and represents a challenging task. The porcine skin ex vivo was topically treated with a gel containing caffeine (CF) and propylene glycol (PG), and depth‐resolved Raman spectra were recorded with two confocal Raman microscopes. We applied a novel tailored multivariate curve resolution‐alternating least squares method to the selected spectral regions (512–604 and 778–1148 cm−1) of gel‐treated skin and quantitatively determined the concentrations of CF and PG in the stratum corneum (SC). The highest concentration of CF (181 mg/cm3) was found at the surface, while PG (384 mg/cm3) was found at 10% SC depth, indicating the formation of a reservoir at the superficial SC. The concentrations of CF and PG decreased monotonically and reached the detection limit at ≈60% and ≈80% SC depth, respectively, indicating that neither permeate the SC.
The novel tailored multivariate curve resolution‐alternating least squares method is used to quantitatively determine the penetration profiles of caffeine (CF) and propylene glycol (PG) applied to the skin in a mixture. PG creates a reservoir at the superficial stratum corneum (SC) depth. The concentrations of CF and PG decrease monotonically and reach the detection limit at a depth of 60% SC depth (≈9 μm) and 80% SC depth (≈12 μm), respectively, indicating that neither permeate the SC.</description><subject>Animals</subject><subject>anti‐aging</subject><subject>Caffeine</subject><subject>cosmetics</subject><subject>depth profiles</subject><subject>drug delivery</subject><subject>Epidermis</subject><subject>keratin</subject><subject>Least squares method</subject><subject>Least-Squares Analysis</subject><subject>Microscopes</subject><subject>Multivariate analysis</subject><subject>penetration pathways</subject><subject>Propylene</subject><subject>Propylene Glycol</subject><subject>Raman micro‐spectroscopy</subject><subject>Raman spectra</subject><subject>Raman spectroscopy</subject><subject>Skin</subject><subject>skin barrier function</subject><subject>spectra normalization</subject><subject>Spectrum Analysis, Raman - methods</subject><subject>Stratum corneum</subject><subject>Swine</subject><subject>transdermal delivery</subject><issn>1864-063X</issn><issn>1864-0648</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAURiMEoqWwZYkssWEzg2Nn7GQJFYWiShWoC3bRjXNdPDh2ajsD2fUReC4egyfBYcogsWHlv-Nzr_0VxdOSrktK2cttZ_yaUcYpLStxrzgua1GtqKjq-4c5_3RUPIpxS6mgfMMfFkdcbjZC8ua4-PFhApeMNgqS8Y54TeIX48iIDlM47CnQGo1DAq4nY_DjbDNAru2svCUwjtZgT5Ifs8famWQDkMF8S1NA0s0kgbE-ZGSYbDI7CAYSEjWFHZKA0dtpqfTz9jvYhMHluu6aWISYSLyZICNLFz2O6XOGft_YZdtHGMCROKLKvT4uHmiwEZ_cjSfF1dmbq9N3q4vLt-enry5WiksuVlIoySmTTc9QY8U6iiXFvlcgNUimN6wRnZawHDadpKIGIXid13UjJfKT4sVem7_hZsKY2sFEhdaCQz_FltVVXVesbjYZff4PuvVTfp3NVJPDoBldqPWeUsHHGFC3YzADhLktabuE3C4ht4eQ84Vnd9qpG7A_4H9SzUCzB74ai_N_dO371-eXf-W_AFkpvGw</recordid><startdate>202312</startdate><enddate>202312</enddate><creator>Choe, ChunSik</creator><creator>Pak, Gyong Jin</creator><creator>Ascencio, Saul Mujica</creator><creator>Darvin, Maxim E.</creator><general>WILEY‐VCH Verlag GmbH & Co. KGaA</general><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7SP</scope><scope>7SR</scope><scope>7U5</scope><scope>8FD</scope><scope>FR3</scope><scope>JG9</scope><scope>K9.</scope><scope>L7M</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-1075-1994</orcidid></search><sort><creationdate>202312</creationdate><title>Quantification of skin penetration of caffeine and propylene glycol applied topically in a mixture by tailored multivariate curve resolution‐alternating least squares of depth‐resolved Raman spectra</title><author>Choe, ChunSik ; Pak, Gyong Jin ; Ascencio, Saul Mujica ; Darvin, Maxim E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3736-76c730279d2efe42b0e10eddca7fa72f5296bf7aefe49b7068a6638aef8977e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animals</topic><topic>anti‐aging</topic><topic>Caffeine</topic><topic>cosmetics</topic><topic>depth profiles</topic><topic>drug delivery</topic><topic>Epidermis</topic><topic>keratin</topic><topic>Least squares method</topic><topic>Least-Squares Analysis</topic><topic>Microscopes</topic><topic>Multivariate analysis</topic><topic>penetration pathways</topic><topic>Propylene</topic><topic>Propylene Glycol</topic><topic>Raman micro‐spectroscopy</topic><topic>Raman spectra</topic><topic>Raman spectroscopy</topic><topic>Skin</topic><topic>skin barrier function</topic><topic>spectra normalization</topic><topic>Spectrum Analysis, Raman - methods</topic><topic>Stratum corneum</topic><topic>Swine</topic><topic>transdermal delivery</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Choe, ChunSik</creatorcontrib><creatorcontrib>Pak, Gyong Jin</creatorcontrib><creatorcontrib>Ascencio, Saul Mujica</creatorcontrib><creatorcontrib>Darvin, Maxim E.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Materials Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biophotonics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Choe, ChunSik</au><au>Pak, Gyong Jin</au><au>Ascencio, Saul Mujica</au><au>Darvin, Maxim E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantification of skin penetration of caffeine and propylene glycol applied topically in a mixture by tailored multivariate curve resolution‐alternating least squares of depth‐resolved Raman spectra</atitle><jtitle>Journal of biophotonics</jtitle><addtitle>J Biophotonics</addtitle><date>2023-12</date><risdate>2023</risdate><volume>16</volume><issue>12</issue><spage>e202300146</spage><epage>n/a</epage><pages>e202300146-n/a</pages><issn>1864-063X</issn><eissn>1864-0648</eissn><abstract>The quantitative determination of topically applied substances in the skin is severely limited and represents a challenging task. The porcine skin ex vivo was topically treated with a gel containing caffeine (CF) and propylene glycol (PG), and depth‐resolved Raman spectra were recorded with two confocal Raman microscopes. We applied a novel tailored multivariate curve resolution‐alternating least squares method to the selected spectral regions (512–604 and 778–1148 cm−1) of gel‐treated skin and quantitatively determined the concentrations of CF and PG in the stratum corneum (SC). The highest concentration of CF (181 mg/cm3) was found at the surface, while PG (384 mg/cm3) was found at 10% SC depth, indicating the formation of a reservoir at the superficial SC. The concentrations of CF and PG decreased monotonically and reached the detection limit at ≈60% and ≈80% SC depth, respectively, indicating that neither permeate the SC.
The novel tailored multivariate curve resolution‐alternating least squares method is used to quantitatively determine the penetration profiles of caffeine (CF) and propylene glycol (PG) applied to the skin in a mixture. PG creates a reservoir at the superficial stratum corneum (SC) depth. The concentrations of CF and PG decrease monotonically and reach the detection limit at a depth of 60% SC depth (≈9 μm) and 80% SC depth (≈12 μm), respectively, indicating that neither permeate the SC.</abstract><cop>Weinheim</cop><pub>WILEY‐VCH Verlag GmbH & Co. KGaA</pub><pmid>37556739</pmid><doi>10.1002/jbio.202300146</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0003-1075-1994</orcidid></addata></record> |
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| source | MEDLINE; Wiley Journals |
| subjects | Animals anti‐aging Caffeine cosmetics depth profiles drug delivery Epidermis keratin Least squares method Least-Squares Analysis Microscopes Multivariate analysis penetration pathways Propylene Propylene Glycol Raman micro‐spectroscopy Raman spectra Raman spectroscopy Skin skin barrier function spectra normalization Spectrum Analysis, Raman - methods Stratum corneum Swine transdermal delivery |
| title | Quantification of skin penetration of caffeine and propylene glycol applied topically in a mixture by tailored multivariate curve resolution‐alternating least squares of depth‐resolved Raman spectra |
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