Combinatorial protein engineering and transporter engineering for efficient synthesis of L-Carnosine in Escherichia coli

•A cell factory of L-Car was built with transporter engineering and protein engineering.•G148D/T168S mutant was first found to significantly increase L-Car yield by 41.6%.•Transporter protein YeaS was identified as involved in L-Car production.•By biocatalysis, the yield of L-Car was 133 mM, which w...

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Veröffentlicht in:	Bioresource technology 2023-11, Vol.387, p.129628-129628, Article 129628
Hauptverfasser:	Liu, Yunran, Pan, Xuewei, Zhang, Hengwei, Zhao, Zhenqiang, Teng, Zixin, Rao, Zhiming
Format:	Artikel
Sprache:	eng
Schlagworte:	Dipeptidase L-Carnosine One-pot biotransformation Protein engineering Transporter engineering
Online-Zugang:	Volltext
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