A dual biomarker-targeting probe enables signal-on surface labeling of Staphylococcus aureus

Imaging or killing of a specific pathogen is of significance for precise therapy. Staphylococcus aureus (S. aureus) is an infectious gram-positive bacteria relying on Sortase A (SrtA) to anchor cell surface protein on peptidoglycan. We herein report signal-on labeling of S. aureus with self-quenched...

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Veröffentlicht in:Bioorganic & medicinal chemistry letters 2023-09, Vol.93, p.129428-129428, Article 129428
Hauptverfasser: Jiang, Feng, Cai, Chengteng, Wang, Xiumin, Han, Shoufa
Format: Artikel
Sprache:eng
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Zusammenfassung:Imaging or killing of a specific pathogen is of significance for precise therapy. Staphylococcus aureus (S. aureus) is an infectious gram-positive bacteria relying on Sortase A (SrtA) to anchor cell surface protein on peptidoglycan. We herein report signal-on labeling of S. aureus with self-quenched optical probes featuring vancomycin-conjugated SrtA substrate that is flanked by a dabcyl moiety paired with either fluorescein or eosine photosensizer (PS). SrtA-mediated cleavage of the substrate motif releases the dabcyl quencher, leading to covalent labeling of peptidoglycan with fluorescein or PS of restored photophysical property. The dual biomarked-enabled peptidoglycan labeling enables signal-on imaging and effective photodynamic destruction of S. aureus, suggesting a protheranostic approch activatable to SrtA-positive bacteria engaged in myriad diseases.
ISSN:0960-894X
1464-3405
DOI:10.1016/j.bmcl.2023.129428