Design of an Antigen-Triggered Nanobody-Based Fluorescence Probe for PET Immunoassay to Detect Quinalphos in Food Samples

Photoinduced electron-transfer (PET) immunoassay based on a fluorescence site-specifically labeled nanobody, also called mini Quenchbody (Q-body), exhibits extraordinary sensitivity and saves much time in the homogeneous noncompetitive mode and is therefore regarded as a valuable method. However, li...

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Veröffentlicht in:	Analytical chemistry (Washington) 2023-08, Vol.95 (33), p.12321-12328
Hauptverfasser:	Liang, Yi-Fan, Li, Jia-Dong, Fang, Ru-Yu, Xu, Zhen-Lin, Luo, Lin, Chen, Zi-Jian, Yang, Jin-Yi, Shen, Yu-Dong, Ueda, Hiroshi, Hammock, Bruce, Wang, Hong
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Antibodies Antigens Fluorescence Fluorescent indicators Immunoassay Liquid chromatography Mass spectrometry Mass spectroscopy Nanobodies Positron emission Quinalphos Sensitivity Site-directed mutagenesis Structural analysis
Online-Zugang:	Volltext
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