Natural regulatory T cells increase significantly in pediatric patients with parasitic infections: Flow cytometry study
Background: The most accepted definition of regulatory T cells (Tregs) relies on the expression of several biomarkers, including CD4, CD25, and transcription factor, Foxp3. The Tregs maintain tolerance to self-antigens and prevent autoimmune diseases. Aim: The purpose of this study was to determine...
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| Veröffentlicht in: | Indian journal of pathology & microbiology 2023-07, Vol.66 (3), p.556-559 |
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| creator | Kizilbash, Nadeem Suhail, Nida Alzahrani, A Basha, W Soliman, Mohamed |
| description | Background: The most accepted definition of regulatory T cells (Tregs) relies on the expression of several biomarkers, including CD4, CD25, and transcription factor, Foxp3. The Tregs maintain tolerance to self-antigens and prevent autoimmune diseases. Aim: The purpose of this study was to determine the difference in natural Treg levels in Entamoeba histolytica, Schistosoma mansoni, Giardia lamblia, Enterobius vermicularis, and Hymenolepis nana infected patients. Setting and Design: Fifty-one pediatric subjects (29 males and 22 females) were recruited from a tertiary care hospital, and were divided into infected and non-infected (control) groups. The mean age of the subjects was 8.7 years. Materials and Methods: Blood samples were collected from infected and non-infected groups, and change in the level of Tregs in these subjects was investigated by flow cytometry. Statistical Analysis Used: The statistical analysis of data was performed by SPSS software. Quantitative data used in this study included mean and standard deviation. Data from the two groups were compared by the Student's t-test. The age of the patient and infection status were used for multivariate logistic regression analysis. Odds ratios (ORs) were estimated within a 95% confidence interval, and a P value of |
| doi_str_mv | 10.4103/ijpm.ijpm_1262_21 |
| format | Article |
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The Tregs maintain tolerance to self-antigens and prevent autoimmune diseases. Aim: The purpose of this study was to determine the difference in natural Treg levels in Entamoeba histolytica, Schistosoma mansoni, Giardia lamblia, Enterobius vermicularis, and Hymenolepis nana infected patients. Setting and Design: Fifty-one pediatric subjects (29 males and 22 females) were recruited from a tertiary care hospital, and were divided into infected and non-infected (control) groups. The mean age of the subjects was 8.7 years. Materials and Methods: Blood samples were collected from infected and non-infected groups, and change in the level of Tregs in these subjects was investigated by flow cytometry. Statistical Analysis Used: The statistical analysis of data was performed by SPSS software. Quantitative data used in this study included mean and standard deviation. Data from the two groups were compared by the Student's t-test. The age of the patient and infection status were used for multivariate logistic regression analysis. Odds ratios (ORs) were estimated within a 95% confidence interval, and a P value of <0.05 was considered significant. Results and Conclusions: The levels of natural regulatory T cells, indicated by the biomarkers, CD4+, CD25+, and Foxp3+, increase significantly in patients infected by Entamoeba histolytica, Schistosoma mansoni, Giardia lamblia, Enterobius vermicularis, and Hymenolepis nana as compared to controls. They also increase in cases of mixed infection as compared to infection by a single parasite.</description><identifier>ISSN: 0377-4929</identifier><identifier>EISSN: 0974-5130</identifier><identifier>DOI: 10.4103/ijpm.ijpm_1262_21</identifier><identifier>PMID: 37530338</identifier><language>eng</language><publisher>India: Wolters Kluwer India Pvt. Ltd</publisher><subject>Antigens ; Autoantigens ; Autoimmune diseases ; Biomarkers ; CD25 antigen ; CD4 antigen ; Children ; Confidence intervals ; Entamoeba histolytica ; Enterobius vermicularis ; Flow cytometry ; Foxp3 protein ; Giardia ; Giardia lamblia ; Health aspects ; Hymenolepis nana ; Immunological tolerance ; Immunoregulation ; Infections ; Lymphocytes ; Lymphocytes T ; Mean ; Medical research ; Medicine, Experimental ; Mixed infection ; Parasites ; Parasitic diseases ; Patients ; Pediatrics ; Protozoa ; Regression analysis ; Schistosoma mansoni ; Statistical analysis ; Statistics ; T cells</subject><ispartof>Indian journal of pathology & microbiology, 2023-07, Vol.66 (3), p.556-559</ispartof><rights>COPYRIGHT 2023 Medknow Publications and Media Pvt. Ltd.</rights><rights>2023. This article is published under (http://creativecommons.org/licenses/by-nc-sa/3.0/) (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c507o-f34ce36c2583cbd350c095cd94993cf2b2fd7513020703e1f1e2c4a15d48d8453</citedby><cites>FETCH-LOGICAL-c507o-f34ce36c2583cbd350c095cd94993cf2b2fd7513020703e1f1e2c4a15d48d8453</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,860,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37530338$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kizilbash, Nadeem</creatorcontrib><creatorcontrib>Suhail, Nida</creatorcontrib><creatorcontrib>Alzahrani, A</creatorcontrib><creatorcontrib>Basha, W</creatorcontrib><creatorcontrib>Soliman, Mohamed</creatorcontrib><title>Natural regulatory T cells increase significantly in pediatric patients with parasitic infections: Flow cytometry study</title><title>Indian journal of pathology & microbiology</title><addtitle>Indian J Pathol Microbiol</addtitle><description>Background: The most accepted definition of regulatory T cells (Tregs) relies on the expression of several biomarkers, including CD4, CD25, and transcription factor, Foxp3. The Tregs maintain tolerance to self-antigens and prevent autoimmune diseases. Aim: The purpose of this study was to determine the difference in natural Treg levels in Entamoeba histolytica, Schistosoma mansoni, Giardia lamblia, Enterobius vermicularis, and Hymenolepis nana infected patients. Setting and Design: Fifty-one pediatric subjects (29 males and 22 females) were recruited from a tertiary care hospital, and were divided into infected and non-infected (control) groups. The mean age of the subjects was 8.7 years. Materials and Methods: Blood samples were collected from infected and non-infected groups, and change in the level of Tregs in these subjects was investigated by flow cytometry. Statistical Analysis Used: The statistical analysis of data was performed by SPSS software. Quantitative data used in this study included mean and standard deviation. Data from the two groups were compared by the Student's t-test. The age of the patient and infection status were used for multivariate logistic regression analysis. Odds ratios (ORs) were estimated within a 95% confidence interval, and a P value of <0.05 was considered significant. Results and Conclusions: The levels of natural regulatory T cells, indicated by the biomarkers, CD4+, CD25+, and Foxp3+, increase significantly in patients infected by Entamoeba histolytica, Schistosoma mansoni, Giardia lamblia, Enterobius vermicularis, and Hymenolepis nana as compared to controls. They also increase in cases of mixed infection as compared to infection by a single parasite.</description><subject>Antigens</subject><subject>Autoantigens</subject><subject>Autoimmune diseases</subject><subject>Biomarkers</subject><subject>CD25 antigen</subject><subject>CD4 antigen</subject><subject>Children</subject><subject>Confidence intervals</subject><subject>Entamoeba histolytica</subject><subject>Enterobius vermicularis</subject><subject>Flow cytometry</subject><subject>Foxp3 protein</subject><subject>Giardia</subject><subject>Giardia lamblia</subject><subject>Health aspects</subject><subject>Hymenolepis nana</subject><subject>Immunological tolerance</subject><subject>Immunoregulation</subject><subject>Infections</subject><subject>Lymphocytes</subject><subject>Lymphocytes T</subject><subject>Mean</subject><subject>Medical research</subject><subject>Medicine, Experimental</subject><subject>Mixed infection</subject><subject>Parasites</subject><subject>Parasitic diseases</subject><subject>Patients</subject><subject>Pediatrics</subject><subject>Protozoa</subject><subject>Regression analysis</subject><subject>Schistosoma mansoni</subject><subject>Statistical analysis</subject><subject>Statistics</subject><subject>T cells</subject><issn>0377-4929</issn><issn>0974-5130</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNp1kk1v1DAQhiNERUvhB3BBlrhwydYfcZxwqyoKiAou5Wx5ncnirWMH21GUf4_DboFWIEv-GD3vqxnPFMUrgjcVwezC7Mdhs26S0JpKSp4UZ7gVVckJw0_znQlRVi1tT4vnMe4xrivK2bPilAnOMGPNWTF_UWkKyqIAu8mq5MOCbpEGayMyTgdQEVA0O2d6o5VLdslhNEJnVApGo1ElAy5FNJv0Pb-CiibluHE96GS8i-_QtfUz0kvyA6RsH9PULS-Kk17ZCC-P53nx7fr97dXH8ubrh09Xlzel5lj4smeVBlZryhumtx3jWOOW666t2pbpnm5p34m1WooFZkB6AlRXivCuarqm4uy8eHvwHYP_MUFMcjBxLU858FOUNEME15ys6JtH6N5PweXsVkqsH4brP9ROWZC5TJ-C0qupvBQ1xrRp6pXa_IPKq4PBaO-gNzn-QEAOAh18jAF6OQYzqLBIguXabPmrz383O2teHxOetgN0vxX33c3A5wMwe5sgxDs7zRBkZu-cn__vLDmv5XEw5P1gsJ9iX8Lr</recordid><startdate>20230701</startdate><enddate>20230701</enddate><creator>Kizilbash, Nadeem</creator><creator>Suhail, Nida</creator><creator>Alzahrani, A</creator><creator>Basha, W</creator><creator>Soliman, Mohamed</creator><general>Wolters Kluwer India Pvt. 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Academic</collection><jtitle>Indian journal of pathology & microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kizilbash, Nadeem</au><au>Suhail, Nida</au><au>Alzahrani, A</au><au>Basha, W</au><au>Soliman, Mohamed</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Natural regulatory T cells increase significantly in pediatric patients with parasitic infections: Flow cytometry study</atitle><jtitle>Indian journal of pathology & microbiology</jtitle><addtitle>Indian J Pathol Microbiol</addtitle><date>2023-07-01</date><risdate>2023</risdate><volume>66</volume><issue>3</issue><spage>556</spage><epage>559</epage><pages>556-559</pages><issn>0377-4929</issn><eissn>0974-5130</eissn><abstract>Background: The most accepted definition of regulatory T cells (Tregs) relies on the expression of several biomarkers, including CD4, CD25, and transcription factor, Foxp3. The Tregs maintain tolerance to self-antigens and prevent autoimmune diseases. Aim: The purpose of this study was to determine the difference in natural Treg levels in Entamoeba histolytica, Schistosoma mansoni, Giardia lamblia, Enterobius vermicularis, and Hymenolepis nana infected patients. Setting and Design: Fifty-one pediatric subjects (29 males and 22 females) were recruited from a tertiary care hospital, and were divided into infected and non-infected (control) groups. The mean age of the subjects was 8.7 years. Materials and Methods: Blood samples were collected from infected and non-infected groups, and change in the level of Tregs in these subjects was investigated by flow cytometry. Statistical Analysis Used: The statistical analysis of data was performed by SPSS software. Quantitative data used in this study included mean and standard deviation. Data from the two groups were compared by the Student's t-test. The age of the patient and infection status were used for multivariate logistic regression analysis. Odds ratios (ORs) were estimated within a 95% confidence interval, and a P value of <0.05 was considered significant. Results and Conclusions: The levels of natural regulatory T cells, indicated by the biomarkers, CD4+, CD25+, and Foxp3+, increase significantly in patients infected by Entamoeba histolytica, Schistosoma mansoni, Giardia lamblia, Enterobius vermicularis, and Hymenolepis nana as compared to controls. They also increase in cases of mixed infection as compared to infection by a single parasite.</abstract><cop>India</cop><pub>Wolters Kluwer India Pvt. Ltd</pub><pmid>37530338</pmid><doi>10.4103/ijpm.ijpm_1262_21</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Antigens Autoantigens Autoimmune diseases Biomarkers CD25 antigen CD4 antigen Children Confidence intervals Entamoeba histolytica Enterobius vermicularis Flow cytometry Foxp3 protein Giardia Giardia lamblia Health aspects Hymenolepis nana Immunological tolerance Immunoregulation Infections Lymphocytes Lymphocytes T Mean Medical research Medicine, Experimental Mixed infection Parasites Parasitic diseases Patients Pediatrics Protozoa Regression analysis Schistosoma mansoni Statistical analysis Statistics T cells |
| title | Natural regulatory T cells increase significantly in pediatric patients with parasitic infections: Flow cytometry study |
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