A single targeted gamma-ray irradiation induced an acute modulation of immune cells and related cytokines in EMT6 mouse-bearing tumour model
BACKGROUND: A complicated interplay between radiation doses, tumour microenvironment (TME), and host immune system is linked to the active participation of immune response. OBJECTIVE: The effects of single targeted 2 Gy and 8 Gy gamma-ray irradiations on the immune cell population (lymphocytes, B-ce...
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| Veröffentlicht in: | Cancer biomarkers : section A of Disease markers 2023-01, Vol.38 (1), p.61-75 |
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| container_title | Cancer biomarkers : section A of Disease markers |
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| creator | Hasan, Nurhaslina Hasani, Narimah Abdul Hamid Omar, Effat Sham, Fatihah Ronny Fuad, Syed Baharom Syed Ahmad Karim, Muhammad Khalis Abdul Ibahim, Mohammad Johari |
| description | BACKGROUND:
A complicated interplay between radiation doses, tumour microenvironment (TME), and host immune system is linked to the active participation of immune response.
OBJECTIVE:
The effects of single targeted 2 Gy and 8 Gy gamma-ray irradiations on the immune cell population (lymphocytes, B-cells, T-cells, neutrophils, eosinophils, and macrophages) in EMT6 mouse-bearing tumour models was investigated.
METHODS:
The effects of both irradiation doses in early (96 hours) and acute phase (5 to 11 days) post-irradiation on immune parameters were monitored in blood circulation and TME using flow cytometry. Simultaneously, selected cytokines related to immune cells within the TME were measured using multiplex ELISA.
RESULTS:
A temporary reduction in systemic total white blood count (TWBC) resulted from an early phase (96 hours) of gamma-ray irradiation at 2 Gy and 8 Gy compared to sham control group. No difference was obtained in the acute phase. Neutrophils dominated among other immune cells in TME in sham control group. Eosinophils in TME was significantly increased after 8 Gy treatment in acute phase compared to sham control (
p
<
0.005). Furthermore, the increment of tumour necrosis (TNF)-
α
, eotaxin and interleukin (IL)-7 (
p
<
0.05) in both treatment groups and phases were associated with anti-tumour activities within TME by gamma-ray irradiation.
CONCLUSION:
The temporary changes in immune cell populations within systemic circulation and TME induced by different doses of gamma-ray irradiation correlated with suppression of several pro-tumorigenic cytokines in mouse-bearing EMT6 tumour models. |
| doi_str_mv | 10.3233/CBM-220268 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_AFRWT</sourceid><recordid>TN_cdi_proquest_miscellaneous_2844093364</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sage_id>10.3233_CBM-220268</sage_id><sourcerecordid>2844093364</sourcerecordid><originalsourceid>FETCH-LOGICAL-c306t-5a3996dfc57d3b81abfb4f1813a80a4f0b95074818bb1231cac070d47756c4793</originalsourceid><addsrcrecordid>eNptkU1rFTEYhYMotlY3_gAJuFCE0XxOMsv20lahxU1dD-8k71xS56MmM4v7H_zRvpepCuIqCefJOQcOY6-l-KiV1p92F7eVUkLV_gk7ld7ZyttGPaW7daYS0uoT9qKUeyGMlqp5zk60s0rJRp-yn-e8pGk_IF8g73HByPcwjlBlOPCUM8QES5onnqa4BlJh4hDWBfk4x3XYtLnnaRzXCXnAYSjERJ6RROLDYZm_pwkLOfDL27uaPq4Fqw4hUzBfVnrnoxsOL9mzHoaCrx7PM_bt6vJu97m6-Xr9ZXd-UwUt6qWyoJumjn2wLurOS-j6zvTSSw1egOlF11jhjJe-66TSMkAQTkTjnK2DcY0-Y-8334c8_1ixLO2YyrE6TEjlWuWNEY3WtSH07T_oPdWdqB1RtbKOoo7Uh40KeS4lY98-5DRCPrRStMeNWtqo3TYi-M2j5dqNGP-gv0ch4N0GFNjj37z_WP0CByWYaA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2862579504</pqid></control><display><type>article</type><title>A single targeted gamma-ray irradiation induced an acute modulation of immune cells and related cytokines in EMT6 mouse-bearing tumour model</title><source>Sage Journals GOLD Open Access 2024</source><creator>Hasan, Nurhaslina ; Hasani, Narimah Abdul Hamid ; Omar, Effat ; Sham, Fatihah Ronny ; Fuad, Syed Baharom Syed Ahmad ; Karim, Muhammad Khalis Abdul ; Ibahim, Mohammad Johari</creator><creatorcontrib>Hasan, Nurhaslina ; Hasani, Narimah Abdul Hamid ; Omar, Effat ; Sham, Fatihah Ronny ; Fuad, Syed Baharom Syed Ahmad ; Karim, Muhammad Khalis Abdul ; Ibahim, Mohammad Johari</creatorcontrib><description>BACKGROUND:
A complicated interplay between radiation doses, tumour microenvironment (TME), and host immune system is linked to the active participation of immune response.
OBJECTIVE:
The effects of single targeted 2 Gy and 8 Gy gamma-ray irradiations on the immune cell population (lymphocytes, B-cells, T-cells, neutrophils, eosinophils, and macrophages) in EMT6 mouse-bearing tumour models was investigated.
METHODS:
The effects of both irradiation doses in early (96 hours) and acute phase (5 to 11 days) post-irradiation on immune parameters were monitored in blood circulation and TME using flow cytometry. Simultaneously, selected cytokines related to immune cells within the TME were measured using multiplex ELISA.
RESULTS:
A temporary reduction in systemic total white blood count (TWBC) resulted from an early phase (96 hours) of gamma-ray irradiation at 2 Gy and 8 Gy compared to sham control group. No difference was obtained in the acute phase. Neutrophils dominated among other immune cells in TME in sham control group. Eosinophils in TME was significantly increased after 8 Gy treatment in acute phase compared to sham control (
p
<
0.005). Furthermore, the increment of tumour necrosis (TNF)-
α
, eotaxin and interleukin (IL)-7 (
p
<
0.05) in both treatment groups and phases were associated with anti-tumour activities within TME by gamma-ray irradiation.
CONCLUSION:
The temporary changes in immune cell populations within systemic circulation and TME induced by different doses of gamma-ray irradiation correlated with suppression of several pro-tumorigenic cytokines in mouse-bearing EMT6 tumour models.</description><identifier>ISSN: 1574-0153</identifier><identifier>EISSN: 1875-8592</identifier><identifier>DOI: 10.3233/CBM-220268</identifier><identifier>PMID: 37522193</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Animal models ; Anticancer properties ; Blood circulation ; Cytokines ; Eosinophils ; Eotaxin ; Flow cytometry ; Gamma rays ; Immune response ; Immune system ; Irradiation ; Leukocytes (eosinophilic) ; Leukocytes (neutrophilic) ; Lymphocytes ; Lymphocytes B ; Lymphocytes T ; Macrophages ; Neutrophils ; Post-irradiation ; Radiation dosage ; Tumor microenvironment ; Tumors ; γ Radiation</subject><ispartof>Cancer biomarkers : section A of Disease markers, 2023-01, Vol.38 (1), p.61-75</ispartof><rights>2023 – IOS Press. All rights reserved.</rights><rights>Copyright IOS Press BV 2023</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c306t-5a3996dfc57d3b81abfb4f1813a80a4f0b95074818bb1231cac070d47756c4793</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.3233/CBM-220268$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.3233/CBM-220268$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,776,780,21945,27830,27901,27902,44921,45309</link.rule.ids><linktorsrc>$$Uhttps://journals.sagepub.com/doi/full/10.3233/CBM-220268?utm_source=summon&utm_medium=discovery-provider$$EView_record_in_SAGE_Publications$$FView_record_in_$$GSAGE_Publications</linktorsrc><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37522193$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hasan, Nurhaslina</creatorcontrib><creatorcontrib>Hasani, Narimah Abdul Hamid</creatorcontrib><creatorcontrib>Omar, Effat</creatorcontrib><creatorcontrib>Sham, Fatihah Ronny</creatorcontrib><creatorcontrib>Fuad, Syed Baharom Syed Ahmad</creatorcontrib><creatorcontrib>Karim, Muhammad Khalis Abdul</creatorcontrib><creatorcontrib>Ibahim, Mohammad Johari</creatorcontrib><title>A single targeted gamma-ray irradiation induced an acute modulation of immune cells and related cytokines in EMT6 mouse-bearing tumour model</title><title>Cancer biomarkers : section A of Disease markers</title><addtitle>Cancer Biomark</addtitle><description>BACKGROUND:
A complicated interplay between radiation doses, tumour microenvironment (TME), and host immune system is linked to the active participation of immune response.
OBJECTIVE:
The effects of single targeted 2 Gy and 8 Gy gamma-ray irradiations on the immune cell population (lymphocytes, B-cells, T-cells, neutrophils, eosinophils, and macrophages) in EMT6 mouse-bearing tumour models was investigated.
METHODS:
The effects of both irradiation doses in early (96 hours) and acute phase (5 to 11 days) post-irradiation on immune parameters were monitored in blood circulation and TME using flow cytometry. Simultaneously, selected cytokines related to immune cells within the TME were measured using multiplex ELISA.
RESULTS:
A temporary reduction in systemic total white blood count (TWBC) resulted from an early phase (96 hours) of gamma-ray irradiation at 2 Gy and 8 Gy compared to sham control group. No difference was obtained in the acute phase. Neutrophils dominated among other immune cells in TME in sham control group. Eosinophils in TME was significantly increased after 8 Gy treatment in acute phase compared to sham control (
p
<
0.005). Furthermore, the increment of tumour necrosis (TNF)-
α
, eotaxin and interleukin (IL)-7 (
p
<
0.05) in both treatment groups and phases were associated with anti-tumour activities within TME by gamma-ray irradiation.
CONCLUSION:
The temporary changes in immune cell populations within systemic circulation and TME induced by different doses of gamma-ray irradiation correlated with suppression of several pro-tumorigenic cytokines in mouse-bearing EMT6 tumour models.</description><subject>Animal models</subject><subject>Anticancer properties</subject><subject>Blood circulation</subject><subject>Cytokines</subject><subject>Eosinophils</subject><subject>Eotaxin</subject><subject>Flow cytometry</subject><subject>Gamma rays</subject><subject>Immune response</subject><subject>Immune system</subject><subject>Irradiation</subject><subject>Leukocytes (eosinophilic)</subject><subject>Leukocytes (neutrophilic)</subject><subject>Lymphocytes</subject><subject>Lymphocytes B</subject><subject>Lymphocytes T</subject><subject>Macrophages</subject><subject>Neutrophils</subject><subject>Post-irradiation</subject><subject>Radiation dosage</subject><subject>Tumor microenvironment</subject><subject>Tumors</subject><subject>γ Radiation</subject><issn>1574-0153</issn><issn>1875-8592</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNptkU1rFTEYhYMotlY3_gAJuFCE0XxOMsv20lahxU1dD-8k71xS56MmM4v7H_zRvpepCuIqCefJOQcOY6-l-KiV1p92F7eVUkLV_gk7ld7ZyttGPaW7daYS0uoT9qKUeyGMlqp5zk60s0rJRp-yn-e8pGk_IF8g73HByPcwjlBlOPCUM8QES5onnqa4BlJh4hDWBfk4x3XYtLnnaRzXCXnAYSjERJ6RROLDYZm_pwkLOfDL27uaPq4Fqw4hUzBfVnrnoxsOL9mzHoaCrx7PM_bt6vJu97m6-Xr9ZXd-UwUt6qWyoJumjn2wLurOS-j6zvTSSw1egOlF11jhjJe-66TSMkAQTkTjnK2DcY0-Y-8334c8_1ixLO2YyrE6TEjlWuWNEY3WtSH07T_oPdWdqB1RtbKOoo7Uh40KeS4lY98-5DRCPrRStMeNWtqo3TYi-M2j5dqNGP-gv0ch4N0GFNjj37z_WP0CByWYaA</recordid><startdate>20230101</startdate><enddate>20230101</enddate><creator>Hasan, Nurhaslina</creator><creator>Hasani, Narimah Abdul Hamid</creator><creator>Omar, Effat</creator><creator>Sham, Fatihah Ronny</creator><creator>Fuad, Syed Baharom Syed Ahmad</creator><creator>Karim, Muhammad Khalis Abdul</creator><creator>Ibahim, Mohammad Johari</creator><general>SAGE Publications</general><general>IOS Press BV</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>C1K</scope><scope>H94</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>20230101</creationdate><title>A single targeted gamma-ray irradiation induced an acute modulation of immune cells and related cytokines in EMT6 mouse-bearing tumour model</title><author>Hasan, Nurhaslina ; Hasani, Narimah Abdul Hamid ; Omar, Effat ; Sham, Fatihah Ronny ; Fuad, Syed Baharom Syed Ahmad ; Karim, Muhammad Khalis Abdul ; Ibahim, Mohammad Johari</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c306t-5a3996dfc57d3b81abfb4f1813a80a4f0b95074818bb1231cac070d47756c4793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animal models</topic><topic>Anticancer properties</topic><topic>Blood circulation</topic><topic>Cytokines</topic><topic>Eosinophils</topic><topic>Eotaxin</topic><topic>Flow cytometry</topic><topic>Gamma rays</topic><topic>Immune response</topic><topic>Immune system</topic><topic>Irradiation</topic><topic>Leukocytes (eosinophilic)</topic><topic>Leukocytes (neutrophilic)</topic><topic>Lymphocytes</topic><topic>Lymphocytes B</topic><topic>Lymphocytes T</topic><topic>Macrophages</topic><topic>Neutrophils</topic><topic>Post-irradiation</topic><topic>Radiation dosage</topic><topic>Tumor microenvironment</topic><topic>Tumors</topic><topic>γ Radiation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hasan, Nurhaslina</creatorcontrib><creatorcontrib>Hasani, Narimah Abdul Hamid</creatorcontrib><creatorcontrib>Omar, Effat</creatorcontrib><creatorcontrib>Sham, Fatihah Ronny</creatorcontrib><creatorcontrib>Fuad, Syed Baharom Syed Ahmad</creatorcontrib><creatorcontrib>Karim, Muhammad Khalis Abdul</creatorcontrib><creatorcontrib>Ibahim, Mohammad Johari</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Cancer biomarkers : section A of Disease markers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Hasan, Nurhaslina</au><au>Hasani, Narimah Abdul Hamid</au><au>Omar, Effat</au><au>Sham, Fatihah Ronny</au><au>Fuad, Syed Baharom Syed Ahmad</au><au>Karim, Muhammad Khalis Abdul</au><au>Ibahim, Mohammad Johari</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A single targeted gamma-ray irradiation induced an acute modulation of immune cells and related cytokines in EMT6 mouse-bearing tumour model</atitle><jtitle>Cancer biomarkers : section A of Disease markers</jtitle><addtitle>Cancer Biomark</addtitle><date>2023-01-01</date><risdate>2023</risdate><volume>38</volume><issue>1</issue><spage>61</spage><epage>75</epage><pages>61-75</pages><issn>1574-0153</issn><eissn>1875-8592</eissn><abstract>BACKGROUND:
A complicated interplay between radiation doses, tumour microenvironment (TME), and host immune system is linked to the active participation of immune response.
OBJECTIVE:
The effects of single targeted 2 Gy and 8 Gy gamma-ray irradiations on the immune cell population (lymphocytes, B-cells, T-cells, neutrophils, eosinophils, and macrophages) in EMT6 mouse-bearing tumour models was investigated.
METHODS:
The effects of both irradiation doses in early (96 hours) and acute phase (5 to 11 days) post-irradiation on immune parameters were monitored in blood circulation and TME using flow cytometry. Simultaneously, selected cytokines related to immune cells within the TME were measured using multiplex ELISA.
RESULTS:
A temporary reduction in systemic total white blood count (TWBC) resulted from an early phase (96 hours) of gamma-ray irradiation at 2 Gy and 8 Gy compared to sham control group. No difference was obtained in the acute phase. Neutrophils dominated among other immune cells in TME in sham control group. Eosinophils in TME was significantly increased after 8 Gy treatment in acute phase compared to sham control (
p
<
0.005). Furthermore, the increment of tumour necrosis (TNF)-
α
, eotaxin and interleukin (IL)-7 (
p
<
0.05) in both treatment groups and phases were associated with anti-tumour activities within TME by gamma-ray irradiation.
CONCLUSION:
The temporary changes in immune cell populations within systemic circulation and TME induced by different doses of gamma-ray irradiation correlated with suppression of several pro-tumorigenic cytokines in mouse-bearing EMT6 tumour models.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>37522193</pmid><doi>10.3233/CBM-220268</doi><tpages>15</tpages></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | ISSN: 1574-0153 |
| ispartof | Cancer biomarkers : section A of Disease markers, 2023-01, Vol.38 (1), p.61-75 |
| issn | 1574-0153 1875-8592 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2844093364 |
| source | Sage Journals GOLD Open Access 2024 |
| subjects | Animal models Anticancer properties Blood circulation Cytokines Eosinophils Eotaxin Flow cytometry Gamma rays Immune response Immune system Irradiation Leukocytes (eosinophilic) Leukocytes (neutrophilic) Lymphocytes Lymphocytes B Lymphocytes T Macrophages Neutrophils Post-irradiation Radiation dosage Tumor microenvironment Tumors γ Radiation |
| title | A single targeted gamma-ray irradiation induced an acute modulation of immune cells and related cytokines in EMT6 mouse-bearing tumour model |
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