Skeletal calcification patterns of batoid, teleost, and mammalian models: Calcified cartilage versus bone matrix
This study compares the skeletal calcification pattern of batoid Raja asterias with the endochondral ossification model of mammalians Homo sapiens and teleost Xiphias gladius. Skeletal mineralization serves to stiffen the mobile elements for locomotion. Histology, histochemistry, heat deproteination...
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| Veröffentlicht in: | Microscopy research and technique 2023-12, Vol.86 (12), p.1568-1582 |
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| creator | Pazzaglia, Ugo E Reguzzoni, Marcella Milanese, Chiara Manconi, Renata Lanteri, Luca Cubeddu, Tiziana Zarattini, Guido Zecca, Piero A Raspanti, Mario |
| description | This study compares the skeletal calcification pattern of batoid Raja asterias with the endochondral ossification model of mammalians Homo sapiens and teleost Xiphias gladius. Skeletal mineralization serves to stiffen the mobile elements for locomotion. Histology, histochemistry, heat deproteination, scanning electron microscopy (SEM)/EDAX analysis, thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), and Fourier transform infrared spectrometry (FTIR) have been applied in the study. H. sapiens and X. gladius bone specimens showed similar profiles, R. asterias calcified cartilage diverges for higher water release and more amorphous bioapatite. In endochondral ossification, fetal calcified cartilage is progressively replaced by bone matrix, while R. asterias calcified cartilage remains un-remodeled throughout the life span. Ca
and PO
concentration in extracellular matrix is suggested to reach the critical salts precipitation point through H
O recall from extracellular matrix into both chondroblasts or osteoblasts. Cartilage organic phase layout and incomplete mineralization allow interstitial fluids diffusion, chondrocytes survival, and growth in a calcified tissue lacking of a vascular and canalicular system. HIGHLIGHTS: Comparative physico-chemical characterization (TGA, DTG and DSC) testifies the mass loss due to water release, collagen and carbonate decomposition of the three tested matrices. R. asterias calcified cartilage water content is higher than that of H. sapiens and X. gladius, as shown by the respectively highest dehydration enthalpy values. Lower crystallinity degree of R. asterias calcified cartilage can be related to the higher amount of collagen in amorphous form than in bone matrix. These data can be discussed in terms of the mechanostat theory (Frost, 1966) or by organic/inorganic phase transformation in the course evolution from fin to limbs. Mineral analysis documented different charactersof R. asterias vs H. sapiens and X. gladius calcified matrix. |
| doi_str_mv | 10.1002/jemt.24388 |
| format | Article |
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and PO
concentration in extracellular matrix is suggested to reach the critical salts precipitation point through H
O recall from extracellular matrix into both chondroblasts or osteoblasts. Cartilage organic phase layout and incomplete mineralization allow interstitial fluids diffusion, chondrocytes survival, and growth in a calcified tissue lacking of a vascular and canalicular system. HIGHLIGHTS: Comparative physico-chemical characterization (TGA, DTG and DSC) testifies the mass loss due to water release, collagen and carbonate decomposition of the three tested matrices. R. asterias calcified cartilage water content is higher than that of H. sapiens and X. gladius, as shown by the respectively highest dehydration enthalpy values. Lower crystallinity degree of R. asterias calcified cartilage can be related to the higher amount of collagen in amorphous form than in bone matrix. These data can be discussed in terms of the mechanostat theory (Frost, 1966) or by organic/inorganic phase transformation in the course evolution from fin to limbs. Mineral analysis documented different charactersof R. asterias vs H. sapiens and X. gladius calcified matrix.</description><identifier>ISSN: 1059-910X</identifier><identifier>EISSN: 1097-0029</identifier><identifier>DOI: 10.1002/jemt.24388</identifier><identifier>PMID: 37493098</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Animals ; Bone Matrix ; Calcification ; Calcification, Physiologic ; Calcinosis ; Calcium ions ; Calorimetry ; Cartilage ; Chondroblasts ; Chondrocytes ; Collagen ; Collagen - analysis ; Dehydration ; Differential scanning calorimetry ; Endochondral bone ; Enthalpy ; Extracellular matrix ; Fetuses ; Fourier transforms ; Histochemistry ; Histology ; Humans ; Infrared analysis ; Infrared spectroscopy ; Life span ; Locomotion ; Mammals ; Mineralization ; Moisture content ; Ossification ; Phase transitions ; Scanning electron microscopy ; Spectrometry ; Thermogravimetric analysis ; Water - analysis ; Water content</subject><ispartof>Microscopy research and technique, 2023-12, Vol.86 (12), p.1568-1582</ispartof><rights>2023 The Authors. Microscopy Research and Technique published by Wiley Periodicals LLC.</rights><rights>2023. This article is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c310t-f7dfb0966c4b987cc3c967cb10040e6014f5278120fff07ab7fbbad793ec58e13</cites><orcidid>0000-0001-6322-1845 ; 0000-0001-7522-8200 ; 0000-0001-9646-4958 ; 0000-0002-3344-2388 ; 0000-0002-3763-6657 ; 0000-0002-6956-0677 ; 0000-0002-7619-8493 ; 0000-0002-0161-2434 ; 0000-0003-3738-6632</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37493098$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pazzaglia, Ugo E</creatorcontrib><creatorcontrib>Reguzzoni, Marcella</creatorcontrib><creatorcontrib>Milanese, Chiara</creatorcontrib><creatorcontrib>Manconi, Renata</creatorcontrib><creatorcontrib>Lanteri, Luca</creatorcontrib><creatorcontrib>Cubeddu, Tiziana</creatorcontrib><creatorcontrib>Zarattini, Guido</creatorcontrib><creatorcontrib>Zecca, Piero A</creatorcontrib><creatorcontrib>Raspanti, Mario</creatorcontrib><title>Skeletal calcification patterns of batoid, teleost, and mammalian models: Calcified cartilage versus bone matrix</title><title>Microscopy research and technique</title><addtitle>Microsc Res Tech</addtitle><description>This study compares the skeletal calcification pattern of batoid Raja asterias with the endochondral ossification model of mammalians Homo sapiens and teleost Xiphias gladius. Skeletal mineralization serves to stiffen the mobile elements for locomotion. Histology, histochemistry, heat deproteination, scanning electron microscopy (SEM)/EDAX analysis, thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), and Fourier transform infrared spectrometry (FTIR) have been applied in the study. H. sapiens and X. gladius bone specimens showed similar profiles, R. asterias calcified cartilage diverges for higher water release and more amorphous bioapatite. In endochondral ossification, fetal calcified cartilage is progressively replaced by bone matrix, while R. asterias calcified cartilage remains un-remodeled throughout the life span. Ca
and PO
concentration in extracellular matrix is suggested to reach the critical salts precipitation point through H
O recall from extracellular matrix into both chondroblasts or osteoblasts. Cartilage organic phase layout and incomplete mineralization allow interstitial fluids diffusion, chondrocytes survival, and growth in a calcified tissue lacking of a vascular and canalicular system. HIGHLIGHTS: Comparative physico-chemical characterization (TGA, DTG and DSC) testifies the mass loss due to water release, collagen and carbonate decomposition of the three tested matrices. R. asterias calcified cartilage water content is higher than that of H. sapiens and X. gladius, as shown by the respectively highest dehydration enthalpy values. Lower crystallinity degree of R. asterias calcified cartilage can be related to the higher amount of collagen in amorphous form than in bone matrix. These data can be discussed in terms of the mechanostat theory (Frost, 1966) or by organic/inorganic phase transformation in the course evolution from fin to limbs. Mineral analysis documented different charactersof R. asterias vs H. sapiens and X. gladius calcified matrix.</description><subject>Animals</subject><subject>Bone Matrix</subject><subject>Calcification</subject><subject>Calcification, Physiologic</subject><subject>Calcinosis</subject><subject>Calcium ions</subject><subject>Calorimetry</subject><subject>Cartilage</subject><subject>Chondroblasts</subject><subject>Chondrocytes</subject><subject>Collagen</subject><subject>Collagen - analysis</subject><subject>Dehydration</subject><subject>Differential scanning calorimetry</subject><subject>Endochondral bone</subject><subject>Enthalpy</subject><subject>Extracellular matrix</subject><subject>Fetuses</subject><subject>Fourier transforms</subject><subject>Histochemistry</subject><subject>Histology</subject><subject>Humans</subject><subject>Infrared analysis</subject><subject>Infrared spectroscopy</subject><subject>Life span</subject><subject>Locomotion</subject><subject>Mammals</subject><subject>Mineralization</subject><subject>Moisture content</subject><subject>Ossification</subject><subject>Phase transitions</subject><subject>Scanning electron microscopy</subject><subject>Spectrometry</subject><subject>Thermogravimetric analysis</subject><subject>Water - analysis</subject><subject>Water content</subject><issn>1059-910X</issn><issn>1097-0029</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkU2LFDEQhoMo7ode_AES8CLL9lrppDsdbzLsh7DgQQVvTZKuSGbTndkkLfrvzTi7e_CUIjz1UFUvIW8YXDCA9sMW53LRCj4Mz8gxAyWb-que7-tONYrBjyNykvMWgLGOiZfkiEuhOKjhmOy-3mHAogO1OljvvNXFx4XudCmYlkyjo0aX6KdzWioZczmnepnorOdZB68XOscJQ_5INwcBTlWVig_6J9JfmPKaqYkL1o6S_O9X5IXTIePrh_eUfL-6_La5aW6_XH_efLptLGdQGicnZ0D1vRVGDdJablUvrakLC8AemHBdKwfWgnMOpDbSGaMnqTjabkDGT8n7g3eX4v2KuYyzzxZD0AvGNY_tIFrRCalURd_9h27jmpY6XaUGpYD3AJU6O1A2xZwTunGX_KzTn5HBuM9h3Ocw_suhwm8flKuZcXpCHw_P_wIg24SR</recordid><startdate>202312</startdate><enddate>202312</enddate><creator>Pazzaglia, Ugo E</creator><creator>Reguzzoni, Marcella</creator><creator>Milanese, Chiara</creator><creator>Manconi, Renata</creator><creator>Lanteri, Luca</creator><creator>Cubeddu, Tiziana</creator><creator>Zarattini, Guido</creator><creator>Zecca, Piero A</creator><creator>Raspanti, Mario</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QP</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7SS</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>7U7</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6322-1845</orcidid><orcidid>https://orcid.org/0000-0001-7522-8200</orcidid><orcidid>https://orcid.org/0000-0001-9646-4958</orcidid><orcidid>https://orcid.org/0000-0002-3344-2388</orcidid><orcidid>https://orcid.org/0000-0002-3763-6657</orcidid><orcidid>https://orcid.org/0000-0002-6956-0677</orcidid><orcidid>https://orcid.org/0000-0002-7619-8493</orcidid><orcidid>https://orcid.org/0000-0002-0161-2434</orcidid><orcidid>https://orcid.org/0000-0003-3738-6632</orcidid></search><sort><creationdate>202312</creationdate><title>Skeletal calcification patterns of batoid, teleost, and mammalian models: Calcified cartilage versus bone matrix</title><author>Pazzaglia, Ugo E ; 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Skeletal mineralization serves to stiffen the mobile elements for locomotion. Histology, histochemistry, heat deproteination, scanning electron microscopy (SEM)/EDAX analysis, thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), and Fourier transform infrared spectrometry (FTIR) have been applied in the study. H. sapiens and X. gladius bone specimens showed similar profiles, R. asterias calcified cartilage diverges for higher water release and more amorphous bioapatite. In endochondral ossification, fetal calcified cartilage is progressively replaced by bone matrix, while R. asterias calcified cartilage remains un-remodeled throughout the life span. Ca
and PO
concentration in extracellular matrix is suggested to reach the critical salts precipitation point through H
O recall from extracellular matrix into both chondroblasts or osteoblasts. Cartilage organic phase layout and incomplete mineralization allow interstitial fluids diffusion, chondrocytes survival, and growth in a calcified tissue lacking of a vascular and canalicular system. HIGHLIGHTS: Comparative physico-chemical characterization (TGA, DTG and DSC) testifies the mass loss due to water release, collagen and carbonate decomposition of the three tested matrices. R. asterias calcified cartilage water content is higher than that of H. sapiens and X. gladius, as shown by the respectively highest dehydration enthalpy values. Lower crystallinity degree of R. asterias calcified cartilage can be related to the higher amount of collagen in amorphous form than in bone matrix. These data can be discussed in terms of the mechanostat theory (Frost, 1966) or by organic/inorganic phase transformation in the course evolution from fin to limbs. Mineral analysis documented different charactersof R. asterias vs H. sapiens and X. gladius calcified matrix.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>37493098</pmid><doi>10.1002/jemt.24388</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0001-6322-1845</orcidid><orcidid>https://orcid.org/0000-0001-7522-8200</orcidid><orcidid>https://orcid.org/0000-0001-9646-4958</orcidid><orcidid>https://orcid.org/0000-0002-3344-2388</orcidid><orcidid>https://orcid.org/0000-0002-3763-6657</orcidid><orcidid>https://orcid.org/0000-0002-6956-0677</orcidid><orcidid>https://orcid.org/0000-0002-7619-8493</orcidid><orcidid>https://orcid.org/0000-0002-0161-2434</orcidid><orcidid>https://orcid.org/0000-0003-3738-6632</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Bone Matrix Calcification Calcification, Physiologic Calcinosis Calcium ions Calorimetry Cartilage Chondroblasts Chondrocytes Collagen Collagen - analysis Dehydration Differential scanning calorimetry Endochondral bone Enthalpy Extracellular matrix Fetuses Fourier transforms Histochemistry Histology Humans Infrared analysis Infrared spectroscopy Life span Locomotion Mammals Mineralization Moisture content Ossification Phase transitions Scanning electron microscopy Spectrometry Thermogravimetric analysis Water - analysis Water content |
| title | Skeletal calcification patterns of batoid, teleost, and mammalian models: Calcified cartilage versus bone matrix |
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