Praeruptorin C alleviates cognitive impairment in type 2 diabetic mice through restoring PI3K/AKT/GSK3β pathway
Diabetic encephalopathy is a common consequence of diabetes mellitus that causes cognitive dysfunction and neuropsychiatric disorders. Praeruptorin C (Pra-C) from the traditional Chinese medicinal herb Peucedanum praeruptorum Dunn. is a potential antioxidant and neuroprotective agent. This study was...
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| Veröffentlicht in: | Phytotherapy research 2023-10, Vol.37 (10), p.4838-4850 |
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| creator | Li, Long-Fei Gao, Ying Xu, Yuan Su, Dan-Jie Yang, Qi Liu, An Wang, Sai-Ying Tang, Xiu-Ling Zhao, Jun Luo, Li Yan, Tao Wu, Yu-Mei Liu, Shui-Bing Zhao, Ming-Gao Yang, Le |
| description | Diabetic encephalopathy is a common consequence of diabetes mellitus that causes cognitive dysfunction and neuropsychiatric disorders. Praeruptorin C (Pra-C) from the traditional Chinese medicinal herb Peucedanum praeruptorum Dunn. is a potential antioxidant and neuroprotective agent. This study was conducted to investigate the molecular mechanisms underlying the effect of Pra-C on diabetic cognitive impairment. A novel object recognition test and the Morris water maze test were performed to assess the behavioral performance of mice. Electrophysiological recordings were made to monitor synaptic plasticity in the hippocampus. A protein-protein interaction network of putative Pra-C targets was constructed, and molecular docking simulations were performed to predict the potential mechanisms of the action of Pra-C. Protein expression levels were detected by western blotting. Pra-C administration significantly lowered body weight and fasting blood glucose levels and alleviated learning and memory deficits in type 2 diabetic mice. Network pharmacology and molecular docking results suggested that Pra-C affects the PI3K/AKT/GSK3β signaling pathway. Western blot analysis confirmed significant increases in phosphorylated PI3K, AKT, and GSK3β levels in vivo and in vitro upon Pra-C administration. Pra-C alleviated cognitive impairment in type 2 diabetic mice by activating PI3K/AKT/GSK3β pathway. |
| doi_str_mv | 10.1002/ptr.7949 |
| format | Article |
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Praeruptorin C (Pra-C) from the traditional Chinese medicinal herb Peucedanum praeruptorum Dunn. is a potential antioxidant and neuroprotective agent. This study was conducted to investigate the molecular mechanisms underlying the effect of Pra-C on diabetic cognitive impairment. A novel object recognition test and the Morris water maze test were performed to assess the behavioral performance of mice. Electrophysiological recordings were made to monitor synaptic plasticity in the hippocampus. A protein-protein interaction network of putative Pra-C targets was constructed, and molecular docking simulations were performed to predict the potential mechanisms of the action of Pra-C. Protein expression levels were detected by western blotting. Pra-C administration significantly lowered body weight and fasting blood glucose levels and alleviated learning and memory deficits in type 2 diabetic mice. Network pharmacology and molecular docking results suggested that Pra-C affects the PI3K/AKT/GSK3β signaling pathway. Western blot analysis confirmed significant increases in phosphorylated PI3K, AKT, and GSK3β levels in vivo and in vitro upon Pra-C administration. Pra-C alleviated cognitive impairment in type 2 diabetic mice by activating PI3K/AKT/GSK3β pathway.</description><identifier>ISSN: 0951-418X</identifier><identifier>EISSN: 1099-1573</identifier><identifier>DOI: 10.1002/ptr.7949</identifier><identifier>PMID: 37458182</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>1-Phosphatidylinositol 3-kinase ; AKT protein ; Body weight ; Cognitive ability ; Diabetes ; Diabetes mellitus ; Encephalopathy ; Herbal medicine ; Hippocampal plasticity ; Impairment ; In vivo methods and tests ; Medicinal plants ; Mental disorders ; Molecular docking ; Molecular modelling ; Neuroprotection ; Object recognition ; Pattern recognition ; Pharmacology ; Proteins ; Signal transduction ; Synaptic plasticity ; Traditional Chinese medicine ; Western blotting</subject><ispartof>Phytotherapy research, 2023-10, Vol.37 (10), p.4838-4850</ispartof><rights>2023 John Wiley & Sons Ltd.</rights><rights>2023 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2609-8e58a227169bd58b724c1bdf07256ee76ac8401c8103bedf1695bf77222585503</citedby><cites>FETCH-LOGICAL-c2609-8e58a227169bd58b724c1bdf07256ee76ac8401c8103bedf1695bf77222585503</cites><orcidid>0000-0002-7539-3887 ; 0000-0003-1706-5241 ; 0000-0001-8959-1342 ; 0000-0002-1217-3705 ; 0000-0002-9325-3474</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37458182$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Long-Fei</creatorcontrib><creatorcontrib>Gao, Ying</creatorcontrib><creatorcontrib>Xu, Yuan</creatorcontrib><creatorcontrib>Su, Dan-Jie</creatorcontrib><creatorcontrib>Yang, Qi</creatorcontrib><creatorcontrib>Liu, An</creatorcontrib><creatorcontrib>Wang, Sai-Ying</creatorcontrib><creatorcontrib>Tang, Xiu-Ling</creatorcontrib><creatorcontrib>Zhao, Jun</creatorcontrib><creatorcontrib>Luo, Li</creatorcontrib><creatorcontrib>Yan, Tao</creatorcontrib><creatorcontrib>Wu, Yu-Mei</creatorcontrib><creatorcontrib>Liu, Shui-Bing</creatorcontrib><creatorcontrib>Zhao, Ming-Gao</creatorcontrib><creatorcontrib>Yang, Le</creatorcontrib><title>Praeruptorin C alleviates cognitive impairment in type 2 diabetic mice through restoring PI3K/AKT/GSK3β pathway</title><title>Phytotherapy research</title><addtitle>Phytother Res</addtitle><description>Diabetic encephalopathy is a common consequence of diabetes mellitus that causes cognitive dysfunction and neuropsychiatric disorders. Praeruptorin C (Pra-C) from the traditional Chinese medicinal herb Peucedanum praeruptorum Dunn. is a potential antioxidant and neuroprotective agent. This study was conducted to investigate the molecular mechanisms underlying the effect of Pra-C on diabetic cognitive impairment. A novel object recognition test and the Morris water maze test were performed to assess the behavioral performance of mice. Electrophysiological recordings were made to monitor synaptic plasticity in the hippocampus. A protein-protein interaction network of putative Pra-C targets was constructed, and molecular docking simulations were performed to predict the potential mechanisms of the action of Pra-C. Protein expression levels were detected by western blotting. Pra-C administration significantly lowered body weight and fasting blood glucose levels and alleviated learning and memory deficits in type 2 diabetic mice. Network pharmacology and molecular docking results suggested that Pra-C affects the PI3K/AKT/GSK3β signaling pathway. Western blot analysis confirmed significant increases in phosphorylated PI3K, AKT, and GSK3β levels in vivo and in vitro upon Pra-C administration. Pra-C alleviated cognitive impairment in type 2 diabetic mice by activating PI3K/AKT/GSK3β pathway.</description><subject>1-Phosphatidylinositol 3-kinase</subject><subject>AKT protein</subject><subject>Body weight</subject><subject>Cognitive ability</subject><subject>Diabetes</subject><subject>Diabetes mellitus</subject><subject>Encephalopathy</subject><subject>Herbal medicine</subject><subject>Hippocampal plasticity</subject><subject>Impairment</subject><subject>In vivo methods and tests</subject><subject>Medicinal plants</subject><subject>Mental disorders</subject><subject>Molecular docking</subject><subject>Molecular modelling</subject><subject>Neuroprotection</subject><subject>Object recognition</subject><subject>Pattern recognition</subject><subject>Pharmacology</subject><subject>Proteins</subject><subject>Signal transduction</subject><subject>Synaptic plasticity</subject><subject>Traditional Chinese medicine</subject><subject>Western blotting</subject><issn>0951-418X</issn><issn>1099-1573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNpdkctKxDAUhoMoOl7AJ5CAGzd1cmmaZCmDN0ZQUMFdSdPTmUhvJqkyr-WD-EzWO7g6m4-Pn_MhtE_JMSWETfvoj6VO9RqaUKJ1QoXk62hCtKBJStXDFtoO4ZEQohlJN9EWl6lQVLEJ6m-8AT_0sfOuxTNs6hqenYkQsO0WrYvuGbBreuN8A23EIxRXPWCGS2cKiM7ixlnAcem7YbHEHsKnaoFvLvl8ejK_m57fzvnbK-5NXL6Y1S7aqEwdYO_77qD7s9O72UVydX1-OTu5SizLiE4UCGUYkzTTRSlUIVlqaVFWRDKRAcjMWJUSahUlvICyGjlRVFIyxoQSgvAddPTl7X33NIyr8sYFC3VtWuiGkDPFNUslZ2JED_-hj93g23HdSEmZ0UyQ7E9ofReChyrvvWuMX-WU5B8V8rFC_lFhRA--hUPRQPkL_rydvwP724HY</recordid><startdate>20231001</startdate><enddate>20231001</enddate><creator>Li, Long-Fei</creator><creator>Gao, Ying</creator><creator>Xu, Yuan</creator><creator>Su, Dan-Jie</creator><creator>Yang, Qi</creator><creator>Liu, An</creator><creator>Wang, Sai-Ying</creator><creator>Tang, Xiu-Ling</creator><creator>Zhao, Jun</creator><creator>Luo, Li</creator><creator>Yan, Tao</creator><creator>Wu, Yu-Mei</creator><creator>Liu, Shui-Bing</creator><creator>Zhao, Ming-Gao</creator><creator>Yang, Le</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-7539-3887</orcidid><orcidid>https://orcid.org/0000-0003-1706-5241</orcidid><orcidid>https://orcid.org/0000-0001-8959-1342</orcidid><orcidid>https://orcid.org/0000-0002-1217-3705</orcidid><orcidid>https://orcid.org/0000-0002-9325-3474</orcidid></search><sort><creationdate>20231001</creationdate><title>Praeruptorin C alleviates cognitive impairment in type 2 diabetic mice through restoring PI3K/AKT/GSK3β pathway</title><author>Li, Long-Fei ; Gao, Ying ; Xu, Yuan ; Su, Dan-Jie ; Yang, Qi ; Liu, An ; Wang, Sai-Ying ; Tang, Xiu-Ling ; Zhao, Jun ; Luo, Li ; Yan, Tao ; Wu, Yu-Mei ; Liu, Shui-Bing ; Zhao, Ming-Gao ; Yang, Le</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2609-8e58a227169bd58b724c1bdf07256ee76ac8401c8103bedf1695bf77222585503</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>1-Phosphatidylinositol 3-kinase</topic><topic>AKT protein</topic><topic>Body weight</topic><topic>Cognitive ability</topic><topic>Diabetes</topic><topic>Diabetes mellitus</topic><topic>Encephalopathy</topic><topic>Herbal medicine</topic><topic>Hippocampal plasticity</topic><topic>Impairment</topic><topic>In vivo methods and tests</topic><topic>Medicinal plants</topic><topic>Mental disorders</topic><topic>Molecular docking</topic><topic>Molecular modelling</topic><topic>Neuroprotection</topic><topic>Object recognition</topic><topic>Pattern recognition</topic><topic>Pharmacology</topic><topic>Proteins</topic><topic>Signal transduction</topic><topic>Synaptic plasticity</topic><topic>Traditional Chinese medicine</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Long-Fei</creatorcontrib><creatorcontrib>Gao, Ying</creatorcontrib><creatorcontrib>Xu, Yuan</creatorcontrib><creatorcontrib>Su, Dan-Jie</creatorcontrib><creatorcontrib>Yang, Qi</creatorcontrib><creatorcontrib>Liu, An</creatorcontrib><creatorcontrib>Wang, Sai-Ying</creatorcontrib><creatorcontrib>Tang, Xiu-Ling</creatorcontrib><creatorcontrib>Zhao, Jun</creatorcontrib><creatorcontrib>Luo, Li</creatorcontrib><creatorcontrib>Yan, Tao</creatorcontrib><creatorcontrib>Wu, Yu-Mei</creatorcontrib><creatorcontrib>Liu, Shui-Bing</creatorcontrib><creatorcontrib>Zhao, Ming-Gao</creatorcontrib><creatorcontrib>Yang, Le</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Phytotherapy research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Long-Fei</au><au>Gao, Ying</au><au>Xu, Yuan</au><au>Su, Dan-Jie</au><au>Yang, Qi</au><au>Liu, An</au><au>Wang, Sai-Ying</au><au>Tang, Xiu-Ling</au><au>Zhao, Jun</au><au>Luo, Li</au><au>Yan, Tao</au><au>Wu, Yu-Mei</au><au>Liu, Shui-Bing</au><au>Zhao, Ming-Gao</au><au>Yang, Le</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Praeruptorin C alleviates cognitive impairment in type 2 diabetic mice through restoring PI3K/AKT/GSK3β pathway</atitle><jtitle>Phytotherapy research</jtitle><addtitle>Phytother Res</addtitle><date>2023-10-01</date><risdate>2023</risdate><volume>37</volume><issue>10</issue><spage>4838</spage><epage>4850</epage><pages>4838-4850</pages><issn>0951-418X</issn><eissn>1099-1573</eissn><abstract>Diabetic encephalopathy is a common consequence of diabetes mellitus that causes cognitive dysfunction and neuropsychiatric disorders. Praeruptorin C (Pra-C) from the traditional Chinese medicinal herb Peucedanum praeruptorum Dunn. is a potential antioxidant and neuroprotective agent. This study was conducted to investigate the molecular mechanisms underlying the effect of Pra-C on diabetic cognitive impairment. A novel object recognition test and the Morris water maze test were performed to assess the behavioral performance of mice. Electrophysiological recordings were made to monitor synaptic plasticity in the hippocampus. A protein-protein interaction network of putative Pra-C targets was constructed, and molecular docking simulations were performed to predict the potential mechanisms of the action of Pra-C. Protein expression levels were detected by western blotting. Pra-C administration significantly lowered body weight and fasting blood glucose levels and alleviated learning and memory deficits in type 2 diabetic mice. Network pharmacology and molecular docking results suggested that Pra-C affects the PI3K/AKT/GSK3β signaling pathway. Western blot analysis confirmed significant increases in phosphorylated PI3K, AKT, and GSK3β levels in vivo and in vitro upon Pra-C administration. Pra-C alleviated cognitive impairment in type 2 diabetic mice by activating PI3K/AKT/GSK3β pathway.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>37458182</pmid><doi>10.1002/ptr.7949</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-7539-3887</orcidid><orcidid>https://orcid.org/0000-0003-1706-5241</orcidid><orcidid>https://orcid.org/0000-0001-8959-1342</orcidid><orcidid>https://orcid.org/0000-0002-1217-3705</orcidid><orcidid>https://orcid.org/0000-0002-9325-3474</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | 1-Phosphatidylinositol 3-kinase AKT protein Body weight Cognitive ability Diabetes Diabetes mellitus Encephalopathy Herbal medicine Hippocampal plasticity Impairment In vivo methods and tests Medicinal plants Mental disorders Molecular docking Molecular modelling Neuroprotection Object recognition Pattern recognition Pharmacology Proteins Signal transduction Synaptic plasticity Traditional Chinese medicine Western blotting |
| title | Praeruptorin C alleviates cognitive impairment in type 2 diabetic mice through restoring PI3K/AKT/GSK3β pathway |
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