Effects of environmental disinfection on microbial population and resistance genes: A case study of the microecology within a panda enclosure
Widespread use of disinfectants raises concerns over their involvement in altering microbial communities and promoting antimicrobial resistance. This study explores the influence of disinfection protocols on microbial populations and resistance genes within an isolated enclosure environment and in t...
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| Veröffentlicht in: | Environmental research 2023-10, Vol.235, p.116662, Article 116662 |
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| creator | Yan, Min Xu, Chunzhong Li, Caiwu Feng, Yongqi Duan, Juntang Zhao, Ke Wu, Daifu Li, Guo Yang, Shengzhi Han, Xinfeng Xie, Yue Huang, Yan Yu, Xiumei Wu, Jiawei Zou, Likou |
| description | Widespread use of disinfectants raises concerns over their involvement in altering microbial communities and promoting antimicrobial resistance. This study explores the influence of disinfection protocols on microbial populations and resistance genes within an isolated enclosure environment and in the gut of giant pandas (GPs) held within. Samples of panda feces, air conditioning ducts, soil and bamboo were collected before and after disinfection. High-throughput sequencing characterized the microbial flora of GP gut and environmental microbes inside the artificial habitat. Microbial cultures showed that Escherichia coli (34.6%), Enterococcus (15.4%) and other pathogenic bacteria deposited in feces and the enclosure. Isolates exhibit a consistent resistance to disinfectant, with the greatest resistance shown to cyanuric acid, and the lowest to glutaraldehyde-dodecyl dimethyl ammonium bromide (GD-DDAB) and dodecyl dimethyl ammonium bromide (DDAB). The total number of the culturable bacteria in soil and bamboo were significantly diminished after disinfection but increased in the gut. After disinfection, the richness (Chao1 index) of environment samples increased significantly (P |
| doi_str_mv | 10.1016/j.envres.2023.116662 |
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•Bacteria from giant pandas and environment have similar trend resistance.•Disinfection decreased the abundance of some pathogens in feces and environment.•Disinfection shapes microbial populations of the giant panda gut and habitat.•Disinfection results in increased antibiotic resistance genes.</description><identifier>ISSN: 0013-9351</identifier><identifier>ISSN: 1096-0953</identifier><identifier>EISSN: 1096-0953</identifier><identifier>DOI: 10.1016/j.envres.2023.116662</identifier><identifier>PMID: 37453509</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>ARGs ; Disinfection ; Environment ; Giant panda ; Gut microbiota</subject><ispartof>Environmental research, 2023-10, Vol.235, p.116662, Article 116662</ispartof><rights>2023 Elsevier Inc.</rights><rights>Copyright © 2023. Published by Elsevier Inc.</rights><rights>Copyright © 2023 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c362t-b301f7a516a02bbe34a1c2d2b0bb86dde05b39c136a85b163a0163eba17dcd8c3</citedby><cites>FETCH-LOGICAL-c362t-b301f7a516a02bbe34a1c2d2b0bb86dde05b39c136a85b163a0163eba17dcd8c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0013935123014664$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37453509$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yan, Min</creatorcontrib><creatorcontrib>Xu, Chunzhong</creatorcontrib><creatorcontrib>Li, Caiwu</creatorcontrib><creatorcontrib>Feng, Yongqi</creatorcontrib><creatorcontrib>Duan, Juntang</creatorcontrib><creatorcontrib>Zhao, Ke</creatorcontrib><creatorcontrib>Wu, Daifu</creatorcontrib><creatorcontrib>Li, Guo</creatorcontrib><creatorcontrib>Yang, Shengzhi</creatorcontrib><creatorcontrib>Han, Xinfeng</creatorcontrib><creatorcontrib>Xie, Yue</creatorcontrib><creatorcontrib>Huang, Yan</creatorcontrib><creatorcontrib>Yu, Xiumei</creatorcontrib><creatorcontrib>Wu, Jiawei</creatorcontrib><creatorcontrib>Zou, Likou</creatorcontrib><title>Effects of environmental disinfection on microbial population and resistance genes: A case study of the microecology within a panda enclosure</title><title>Environmental research</title><addtitle>Environ Res</addtitle><description>Widespread use of disinfectants raises concerns over their involvement in altering microbial communities and promoting antimicrobial resistance. This study explores the influence of disinfection protocols on microbial populations and resistance genes within an isolated enclosure environment and in the gut of giant pandas (GPs) held within. Samples of panda feces, air conditioning ducts, soil and bamboo were collected before and after disinfection. High-throughput sequencing characterized the microbial flora of GP gut and environmental microbes inside the artificial habitat. Microbial cultures showed that Escherichia coli (34.6%), Enterococcus (15.4%) and other pathogenic bacteria deposited in feces and the enclosure. Isolates exhibit a consistent resistance to disinfectant, with the greatest resistance shown to cyanuric acid, and the lowest to glutaraldehyde-dodecyl dimethyl ammonium bromide (GD-DDAB) and dodecyl dimethyl ammonium bromide (DDAB). The total number of the culturable bacteria in soil and bamboo were significantly diminished after disinfection but increased in the gut. After disinfection, the richness (Chao1 index) of environment samples increased significantly (P < 0.05), while the richness in gut decreased significantly (P < 0.05). Ten genera showed significant change in feces after disinfection. Metagenome sequencing showed that 126 types of virulence genes were present in feces before disinfection and 37 in soil. After disinfection, 110 virulence genes localized in feces and 53 in soil. Eleven virulence genes including ECP and T2SS increased in feces. A total of 182 antibiotic resistance genes (ARGs) subtypes, potentially conferring resistance to 20 classes of drugs, were detected in the soils and feces, with most belonging to efflux pump protein pathways. After disinfection, the number of resistance genes increased both in gut and soil, which suggests disinfection protocols increase the number of resistance pathways. Our study shows that the use of disinfectants helps to shape the microbial community of GPs and their habitat, and increases populations of resistant strain bacteria.
[Display omitted]
•Bacteria from giant pandas and environment have similar trend resistance.•Disinfection decreased the abundance of some pathogens in feces and environment.•Disinfection shapes microbial populations of the giant panda gut and habitat.•Disinfection results in increased antibiotic resistance genes.</description><subject>ARGs</subject><subject>Disinfection</subject><subject>Environment</subject><subject>Giant panda</subject><subject>Gut microbiota</subject><issn>0013-9351</issn><issn>1096-0953</issn><issn>1096-0953</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNp9UctuFDEQtBCILAt_gJCPXGbxY-2d4YAURSEgReICZ8uPnsSrGXtwzwTtR_DPeDOBI5Ily91dVe0qQt5ytuOM6w_HHaSHArgTTMgd51pr8YxsOOt0wzoln5MNY1w2nVT8grxCPNYnV5K9JBfysFdSsW5Dfl_3PfgZae5p5YslpxHSbAcaIsZ07sWcaD1j9CW7WDtTnpbBPtZtCrTuEHG2yQO9gwT4kV5SbxEozks4nYnne1jh4POQ7070V5zvY0XTqRLYKuyHjEuB1-RFbweEN0_3lvz4fP396ktz--3m69XlbeOlFnPjJOP9wSquLRPOgdxb7kUQjjnX6hCAKSc7z6W2rXJcS1sNk-AsPwQfWi-35P3KO5X8cwGczRjRwzDYBHlBI1rZCrVvq11bsl9H6_qIBXozlTjacjKcmXMQ5mjWIMw5CLMGUWHvnhQWN0L4B_rrfB34tA5A_edDhGLQx2oEhFiq6Sbk-H-FP-5cnu8</recordid><startdate>20231015</startdate><enddate>20231015</enddate><creator>Yan, Min</creator><creator>Xu, Chunzhong</creator><creator>Li, Caiwu</creator><creator>Feng, Yongqi</creator><creator>Duan, Juntang</creator><creator>Zhao, Ke</creator><creator>Wu, Daifu</creator><creator>Li, Guo</creator><creator>Yang, Shengzhi</creator><creator>Han, Xinfeng</creator><creator>Xie, Yue</creator><creator>Huang, Yan</creator><creator>Yu, Xiumei</creator><creator>Wu, Jiawei</creator><creator>Zou, Likou</creator><general>Elsevier Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20231015</creationdate><title>Effects of environmental disinfection on microbial population and resistance genes: A case study of the microecology within a panda enclosure</title><author>Yan, Min ; Xu, Chunzhong ; Li, Caiwu ; Feng, Yongqi ; Duan, Juntang ; Zhao, Ke ; Wu, Daifu ; Li, Guo ; Yang, Shengzhi ; Han, Xinfeng ; Xie, Yue ; Huang, Yan ; Yu, Xiumei ; Wu, Jiawei ; Zou, Likou</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c362t-b301f7a516a02bbe34a1c2d2b0bb86dde05b39c136a85b163a0163eba17dcd8c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>ARGs</topic><topic>Disinfection</topic><topic>Environment</topic><topic>Giant panda</topic><topic>Gut microbiota</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yan, Min</creatorcontrib><creatorcontrib>Xu, Chunzhong</creatorcontrib><creatorcontrib>Li, Caiwu</creatorcontrib><creatorcontrib>Feng, Yongqi</creatorcontrib><creatorcontrib>Duan, Juntang</creatorcontrib><creatorcontrib>Zhao, Ke</creatorcontrib><creatorcontrib>Wu, Daifu</creatorcontrib><creatorcontrib>Li, Guo</creatorcontrib><creatorcontrib>Yang, Shengzhi</creatorcontrib><creatorcontrib>Han, Xinfeng</creatorcontrib><creatorcontrib>Xie, Yue</creatorcontrib><creatorcontrib>Huang, Yan</creatorcontrib><creatorcontrib>Yu, Xiumei</creatorcontrib><creatorcontrib>Wu, Jiawei</creatorcontrib><creatorcontrib>Zou, Likou</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Environmental research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yan, Min</au><au>Xu, Chunzhong</au><au>Li, Caiwu</au><au>Feng, Yongqi</au><au>Duan, Juntang</au><au>Zhao, Ke</au><au>Wu, Daifu</au><au>Li, Guo</au><au>Yang, Shengzhi</au><au>Han, Xinfeng</au><au>Xie, Yue</au><au>Huang, Yan</au><au>Yu, Xiumei</au><au>Wu, Jiawei</au><au>Zou, Likou</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of environmental disinfection on microbial population and resistance genes: A case study of the microecology within a panda enclosure</atitle><jtitle>Environmental research</jtitle><addtitle>Environ Res</addtitle><date>2023-10-15</date><risdate>2023</risdate><volume>235</volume><spage>116662</spage><pages>116662-</pages><artnum>116662</artnum><issn>0013-9351</issn><issn>1096-0953</issn><eissn>1096-0953</eissn><abstract>Widespread use of disinfectants raises concerns over their involvement in altering microbial communities and promoting antimicrobial resistance. This study explores the influence of disinfection protocols on microbial populations and resistance genes within an isolated enclosure environment and in the gut of giant pandas (GPs) held within. Samples of panda feces, air conditioning ducts, soil and bamboo were collected before and after disinfection. High-throughput sequencing characterized the microbial flora of GP gut and environmental microbes inside the artificial habitat. Microbial cultures showed that Escherichia coli (34.6%), Enterococcus (15.4%) and other pathogenic bacteria deposited in feces and the enclosure. Isolates exhibit a consistent resistance to disinfectant, with the greatest resistance shown to cyanuric acid, and the lowest to glutaraldehyde-dodecyl dimethyl ammonium bromide (GD-DDAB) and dodecyl dimethyl ammonium bromide (DDAB). The total number of the culturable bacteria in soil and bamboo were significantly diminished after disinfection but increased in the gut. After disinfection, the richness (Chao1 index) of environment samples increased significantly (P < 0.05), while the richness in gut decreased significantly (P < 0.05). Ten genera showed significant change in feces after disinfection. Metagenome sequencing showed that 126 types of virulence genes were present in feces before disinfection and 37 in soil. After disinfection, 110 virulence genes localized in feces and 53 in soil. Eleven virulence genes including ECP and T2SS increased in feces. A total of 182 antibiotic resistance genes (ARGs) subtypes, potentially conferring resistance to 20 classes of drugs, were detected in the soils and feces, with most belonging to efflux pump protein pathways. After disinfection, the number of resistance genes increased both in gut and soil, which suggests disinfection protocols increase the number of resistance pathways. Our study shows that the use of disinfectants helps to shape the microbial community of GPs and their habitat, and increases populations of resistant strain bacteria.
[Display omitted]
•Bacteria from giant pandas and environment have similar trend resistance.•Disinfection decreased the abundance of some pathogens in feces and environment.•Disinfection shapes microbial populations of the giant panda gut and habitat.•Disinfection results in increased antibiotic resistance genes.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>37453509</pmid><doi>10.1016/j.envres.2023.116662</doi></addata></record> |
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| subjects | ARGs Disinfection Environment Giant panda Gut microbiota |
| title | Effects of environmental disinfection on microbial population and resistance genes: A case study of the microecology within a panda enclosure |
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