Effect of human periodontal ligament stem cell‐derived exosomes on cementoblast activity
Objectives Exosomes derived from stem cells are a potential cell‐free tool for tissue regeneration with therapeutic potential. However, its application in cementum repair is unclear. This study aimed to investigate the effect of human periodontal ligament stem cell‐derived exosomes on the biological...
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| Veröffentlicht in: | Oral diseases 2024-05, Vol.30 (4), p.2511-2522 |
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| container_title | Oral diseases |
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| creator | Li, Shengnan Guan, Xiuchen Yu, Wenting Zhao, Zeqing Sun, Yaxi Bai, Yuxing |
| description | Objectives
Exosomes derived from stem cells are a potential cell‐free tool for tissue regeneration with therapeutic potential. However, its application in cementum repair is unclear. This study aimed to investigate the effect of human periodontal ligament stem cell‐derived exosomes on the biological activity of cementoblasts, the main effector cells in cementum synthesis.
Materials and Methods
OCCM‐30 cementoblasts were cultured with various human periodontal ligament stem cell‐derived exosome concentrations. OCCM‐30 cells proliferation, migration, and cementogenic mineralization were examined, along with the gene and protein expression of factors associated with cementoblastic mineralization.
Results
Exosomal promoted the migration, proliferation, and mineralization of OCCM‐30 cells. The exosome‐treated group significantly increased the expression of cementogenic‐related genes and proteins. Furthermore, the expression of p‐PI3K and p‐AKT was enhanced by exosome administration. Treatment with a PI3K/AKT inhibitor markedly attenuated the gene and protein expression of cementoblastic factors, and this effect was partially reversed by exosome administration.
Conclusions
Human periodontal ligament stem cell‐derived exosomes can promote the activity of cementoblasts via the PI3K/AKT signaling pathway, providing a scientific basis for promoting the repair process in orthodontically induced inflammatory root resorption. |
| doi_str_mv | 10.1111/odi.14671 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2838248716</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3062449973</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3531-527e1d97eb1896e20a614059a99c6543ac340b1a6079b00680907cd5133d63823</originalsourceid><addsrcrecordid>eNp10MFKwzAcBvAgipvTgy8gAS966EyaNGmOMqcOBrsoiJeQtv9qR9vMpp3u5iP4jD6JmZseBHNJID8-Pj6EjikZUn8ubFYMKReS7qA-FYQGJA6jXf9mEQ-ikD300IFzc0KoVCzcRz0mOY9DEvXR4zjPIW2xzfFzV5kaL6ApbGbr1pS4LJ5MBXWLXQsVTqEsP98_Mg-WkGF4s85W4LCt_dea2aQ0rsUmbYtl0a4O0V5uSgdH23uA7q_Hd6PbYDq7mYwup0HKIkZ9Pwk0UxISGisBITGCchIpo1QqIs5MyjhJqBFEqoQQERNFZJpFlLFMsDhkA3S2yV009qUD1-qqcOuypgbbOR3GXvFYUuHp6R86t11T-3aaERFyrpRkXp1vVNpY5xrI9aIpKtOsNCV6Pbj2g-vvwb092SZ2SQXZr_xZ2IOLDXgtSlj9n6RnV5NN5BfE5YmG</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3062449973</pqid></control><display><type>article</type><title>Effect of human periodontal ligament stem cell‐derived exosomes on cementoblast activity</title><source>MEDLINE</source><source>Wiley Journals</source><creator>Li, Shengnan ; Guan, Xiuchen ; Yu, Wenting ; Zhao, Zeqing ; Sun, Yaxi ; Bai, Yuxing</creator><creatorcontrib>Li, Shengnan ; Guan, Xiuchen ; Yu, Wenting ; Zhao, Zeqing ; Sun, Yaxi ; Bai, Yuxing</creatorcontrib><description>Objectives
Exosomes derived from stem cells are a potential cell‐free tool for tissue regeneration with therapeutic potential. However, its application in cementum repair is unclear. This study aimed to investigate the effect of human periodontal ligament stem cell‐derived exosomes on the biological activity of cementoblasts, the main effector cells in cementum synthesis.
Materials and Methods
OCCM‐30 cementoblasts were cultured with various human periodontal ligament stem cell‐derived exosome concentrations. OCCM‐30 cells proliferation, migration, and cementogenic mineralization were examined, along with the gene and protein expression of factors associated with cementoblastic mineralization.
Results
Exosomal promoted the migration, proliferation, and mineralization of OCCM‐30 cells. The exosome‐treated group significantly increased the expression of cementogenic‐related genes and proteins. Furthermore, the expression of p‐PI3K and p‐AKT was enhanced by exosome administration. Treatment with a PI3K/AKT inhibitor markedly attenuated the gene and protein expression of cementoblastic factors, and this effect was partially reversed by exosome administration.
Conclusions
Human periodontal ligament stem cell‐derived exosomes can promote the activity of cementoblasts via the PI3K/AKT signaling pathway, providing a scientific basis for promoting the repair process in orthodontically induced inflammatory root resorption.</description><identifier>ISSN: 1354-523X</identifier><identifier>ISSN: 1601-0825</identifier><identifier>EISSN: 1601-0825</identifier><identifier>DOI: 10.1111/odi.14671</identifier><identifier>PMID: 37448205</identifier><language>eng</language><publisher>Denmark: Wiley Subscription Services, Inc</publisher><subject>1-Phosphatidylinositol 3-kinase ; AKT protein ; Animals ; Biological activity ; Cell Line ; Cell migration ; Cell Movement ; Cell Proliferation ; Cells, Cultured ; Cementogenesis ; Cementum ; Dental Cementum - cytology ; Dental Cementum - metabolism ; Effector cells ; Exosomes ; Exosomes - metabolism ; human periodontal ligament stem cells ; Humans ; Inflammation ; Ligaments ; Mice ; Mineralization ; Periodontal ligament ; Periodontal Ligament - cytology ; Periodontal Ligament - metabolism ; Periodontics ; Phosphatidylinositol 3-Kinases - metabolism ; PI3K/AKT pathway ; Protein expression ; Proto-Oncogene Proteins c-akt - metabolism ; Root resorption ; Signal Transduction ; Stem cells ; Stem Cells - metabolism</subject><ispartof>Oral diseases, 2024-05, Vol.30 (4), p.2511-2522</ispartof><rights>2023 Wiley Periodicals LLC.</rights><rights>2024 Wiley Periodicals LLC.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3531-527e1d97eb1896e20a614059a99c6543ac340b1a6079b00680907cd5133d63823</citedby><cites>FETCH-LOGICAL-c3531-527e1d97eb1896e20a614059a99c6543ac340b1a6079b00680907cd5133d63823</cites><orcidid>0000-0001-7159-9661 ; 0000-0002-7239-0891 ; 0000-0002-8189-1233</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fodi.14671$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fodi.14671$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37448205$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Shengnan</creatorcontrib><creatorcontrib>Guan, Xiuchen</creatorcontrib><creatorcontrib>Yu, Wenting</creatorcontrib><creatorcontrib>Zhao, Zeqing</creatorcontrib><creatorcontrib>Sun, Yaxi</creatorcontrib><creatorcontrib>Bai, Yuxing</creatorcontrib><title>Effect of human periodontal ligament stem cell‐derived exosomes on cementoblast activity</title><title>Oral diseases</title><addtitle>Oral Dis</addtitle><description>Objectives
Exosomes derived from stem cells are a potential cell‐free tool for tissue regeneration with therapeutic potential. However, its application in cementum repair is unclear. This study aimed to investigate the effect of human periodontal ligament stem cell‐derived exosomes on the biological activity of cementoblasts, the main effector cells in cementum synthesis.
Materials and Methods
OCCM‐30 cementoblasts were cultured with various human periodontal ligament stem cell‐derived exosome concentrations. OCCM‐30 cells proliferation, migration, and cementogenic mineralization were examined, along with the gene and protein expression of factors associated with cementoblastic mineralization.
Results
Exosomal promoted the migration, proliferation, and mineralization of OCCM‐30 cells. The exosome‐treated group significantly increased the expression of cementogenic‐related genes and proteins. Furthermore, the expression of p‐PI3K and p‐AKT was enhanced by exosome administration. Treatment with a PI3K/AKT inhibitor markedly attenuated the gene and protein expression of cementoblastic factors, and this effect was partially reversed by exosome administration.
Conclusions
Human periodontal ligament stem cell‐derived exosomes can promote the activity of cementoblasts via the PI3K/AKT signaling pathway, providing a scientific basis for promoting the repair process in orthodontically induced inflammatory root resorption.</description><subject>1-Phosphatidylinositol 3-kinase</subject><subject>AKT protein</subject><subject>Animals</subject><subject>Biological activity</subject><subject>Cell Line</subject><subject>Cell migration</subject><subject>Cell Movement</subject><subject>Cell Proliferation</subject><subject>Cells, Cultured</subject><subject>Cementogenesis</subject><subject>Cementum</subject><subject>Dental Cementum - cytology</subject><subject>Dental Cementum - metabolism</subject><subject>Effector cells</subject><subject>Exosomes</subject><subject>Exosomes - metabolism</subject><subject>human periodontal ligament stem cells</subject><subject>Humans</subject><subject>Inflammation</subject><subject>Ligaments</subject><subject>Mice</subject><subject>Mineralization</subject><subject>Periodontal ligament</subject><subject>Periodontal Ligament - cytology</subject><subject>Periodontal Ligament - metabolism</subject><subject>Periodontics</subject><subject>Phosphatidylinositol 3-Kinases - metabolism</subject><subject>PI3K/AKT pathway</subject><subject>Protein expression</subject><subject>Proto-Oncogene Proteins c-akt - metabolism</subject><subject>Root resorption</subject><subject>Signal Transduction</subject><subject>Stem cells</subject><subject>Stem Cells - metabolism</subject><issn>1354-523X</issn><issn>1601-0825</issn><issn>1601-0825</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10MFKwzAcBvAgipvTgy8gAS966EyaNGmOMqcOBrsoiJeQtv9qR9vMpp3u5iP4jD6JmZseBHNJID8-Pj6EjikZUn8ubFYMKReS7qA-FYQGJA6jXf9mEQ-ikD300IFzc0KoVCzcRz0mOY9DEvXR4zjPIW2xzfFzV5kaL6ApbGbr1pS4LJ5MBXWLXQsVTqEsP98_Mg-WkGF4s85W4LCt_dea2aQ0rsUmbYtl0a4O0V5uSgdH23uA7q_Hd6PbYDq7mYwup0HKIkZ9Pwk0UxISGisBITGCchIpo1QqIs5MyjhJqBFEqoQQERNFZJpFlLFMsDhkA3S2yV009qUD1-qqcOuypgbbOR3GXvFYUuHp6R86t11T-3aaERFyrpRkXp1vVNpY5xrI9aIpKtOsNCV6Pbj2g-vvwb092SZ2SQXZr_xZ2IOLDXgtSlj9n6RnV5NN5BfE5YmG</recordid><startdate>202405</startdate><enddate>202405</enddate><creator>Li, Shengnan</creator><creator>Guan, Xiuchen</creator><creator>Yu, Wenting</creator><creator>Zhao, Zeqing</creator><creator>Sun, Yaxi</creator><creator>Bai, Yuxing</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-7159-9661</orcidid><orcidid>https://orcid.org/0000-0002-7239-0891</orcidid><orcidid>https://orcid.org/0000-0002-8189-1233</orcidid></search><sort><creationdate>202405</creationdate><title>Effect of human periodontal ligament stem cell‐derived exosomes on cementoblast activity</title><author>Li, Shengnan ; Guan, Xiuchen ; Yu, Wenting ; Zhao, Zeqing ; Sun, Yaxi ; Bai, Yuxing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3531-527e1d97eb1896e20a614059a99c6543ac340b1a6079b00680907cd5133d63823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>1-Phosphatidylinositol 3-kinase</topic><topic>AKT protein</topic><topic>Animals</topic><topic>Biological activity</topic><topic>Cell Line</topic><topic>Cell migration</topic><topic>Cell Movement</topic><topic>Cell Proliferation</topic><topic>Cells, Cultured</topic><topic>Cementogenesis</topic><topic>Cementum</topic><topic>Dental Cementum - cytology</topic><topic>Dental Cementum - metabolism</topic><topic>Effector cells</topic><topic>Exosomes</topic><topic>Exosomes - metabolism</topic><topic>human periodontal ligament stem cells</topic><topic>Humans</topic><topic>Inflammation</topic><topic>Ligaments</topic><topic>Mice</topic><topic>Mineralization</topic><topic>Periodontal ligament</topic><topic>Periodontal Ligament - cytology</topic><topic>Periodontal Ligament - metabolism</topic><topic>Periodontics</topic><topic>Phosphatidylinositol 3-Kinases - metabolism</topic><topic>PI3K/AKT pathway</topic><topic>Protein expression</topic><topic>Proto-Oncogene Proteins c-akt - metabolism</topic><topic>Root resorption</topic><topic>Signal Transduction</topic><topic>Stem cells</topic><topic>Stem Cells - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Shengnan</creatorcontrib><creatorcontrib>Guan, Xiuchen</creatorcontrib><creatorcontrib>Yu, Wenting</creatorcontrib><creatorcontrib>Zhao, Zeqing</creatorcontrib><creatorcontrib>Sun, Yaxi</creatorcontrib><creatorcontrib>Bai, Yuxing</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Oral diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Shengnan</au><au>Guan, Xiuchen</au><au>Yu, Wenting</au><au>Zhao, Zeqing</au><au>Sun, Yaxi</au><au>Bai, Yuxing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of human periodontal ligament stem cell‐derived exosomes on cementoblast activity</atitle><jtitle>Oral diseases</jtitle><addtitle>Oral Dis</addtitle><date>2024-05</date><risdate>2024</risdate><volume>30</volume><issue>4</issue><spage>2511</spage><epage>2522</epage><pages>2511-2522</pages><issn>1354-523X</issn><issn>1601-0825</issn><eissn>1601-0825</eissn><abstract>Objectives
Exosomes derived from stem cells are a potential cell‐free tool for tissue regeneration with therapeutic potential. However, its application in cementum repair is unclear. This study aimed to investigate the effect of human periodontal ligament stem cell‐derived exosomes on the biological activity of cementoblasts, the main effector cells in cementum synthesis.
Materials and Methods
OCCM‐30 cementoblasts were cultured with various human periodontal ligament stem cell‐derived exosome concentrations. OCCM‐30 cells proliferation, migration, and cementogenic mineralization were examined, along with the gene and protein expression of factors associated with cementoblastic mineralization.
Results
Exosomal promoted the migration, proliferation, and mineralization of OCCM‐30 cells. The exosome‐treated group significantly increased the expression of cementogenic‐related genes and proteins. Furthermore, the expression of p‐PI3K and p‐AKT was enhanced by exosome administration. Treatment with a PI3K/AKT inhibitor markedly attenuated the gene and protein expression of cementoblastic factors, and this effect was partially reversed by exosome administration.
Conclusions
Human periodontal ligament stem cell‐derived exosomes can promote the activity of cementoblasts via the PI3K/AKT signaling pathway, providing a scientific basis for promoting the repair process in orthodontically induced inflammatory root resorption.</abstract><cop>Denmark</cop><pub>Wiley Subscription Services, Inc</pub><pmid>37448205</pmid><doi>10.1111/odi.14671</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0001-7159-9661</orcidid><orcidid>https://orcid.org/0000-0002-7239-0891</orcidid><orcidid>https://orcid.org/0000-0002-8189-1233</orcidid></addata></record> |
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| ispartof | Oral diseases, 2024-05, Vol.30 (4), p.2511-2522 |
| issn | 1354-523X 1601-0825 1601-0825 |
| language | eng |
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| source | MEDLINE; Wiley Journals |
| subjects | 1-Phosphatidylinositol 3-kinase AKT protein Animals Biological activity Cell Line Cell migration Cell Movement Cell Proliferation Cells, Cultured Cementogenesis Cementum Dental Cementum - cytology Dental Cementum - metabolism Effector cells Exosomes Exosomes - metabolism human periodontal ligament stem cells Humans Inflammation Ligaments Mice Mineralization Periodontal ligament Periodontal Ligament - cytology Periodontal Ligament - metabolism Periodontics Phosphatidylinositol 3-Kinases - metabolism PI3K/AKT pathway Protein expression Proto-Oncogene Proteins c-akt - metabolism Root resorption Signal Transduction Stem cells Stem Cells - metabolism |
| title | Effect of human periodontal ligament stem cell‐derived exosomes on cementoblast activity |
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