Comparative transcriptome analysis on wild-simulated ginseng of different age revealed possible mechanism of ginsenoside accumulation

Panax ginseng is one of the most famous pharmaceutical plants in Asia. Ginseng plants grown in mountain have longer longevity which ensures higher accumulation of ginsenoside components than those grown in farms. However, wild-simulated ginseng over certain age cannot be easily distinguished in morp...

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Veröffentlicht in:Plant physiology and biochemistry 2023-08, Vol.201, p.107870-107870, Article 107870
Hauptverfasser: Kim, Jaewook, Yun, Yeongbae, Huh, Jeonghoon, Um, Yurry, Shim, Donghwan
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Yun, Yeongbae
Huh, Jeonghoon
Um, Yurry
Shim, Donghwan
description Panax ginseng is one of the most famous pharmaceutical plants in Asia. Ginseng plants grown in mountain have longer longevity which ensures higher accumulation of ginsenoside components than those grown in farms. However, wild-simulated ginseng over certain age cannot be easily distinguished in morphology. To identify transcriptomic mechanism of ginsenoside accumulation in older wild-simulated ginseng without large phenotype change, we performed comparative transcriptome analysis for leaf, shoot, and root tissues of 7-yr-old and 13yr-old wild-simulated ginseng. Of 559 differentially expressed genes (DEGs) in comparison between 7-yr-old and 13yr-old wild-simulated ginseng, 280 leaf-, 103 shoot-, and 164 root-mainly expressing genes were found to be changed in transcript level according to age. Functional analysis revealed that pentose-phosphate shunt and abscisic acid responsive genes were up-regulated in leaf tissues of 7-yr-old ginseng while defense responsive genes were up-regulated in root tissues of 13-yr-old ginseng. Quantitative real-time PCR revealed that jasmonic acid responsive genes, ERDL6, and some UGTs were up-regulated in 13-yr-old ginseng in higher order lateral root tissues. These data suggest that bacterial stimulation in mountain region can enhance the expression of several genes which might support minor ginsenoside biosynthesis. [Display omitted] •Older wild-simulated ginseng had higher amount of ginsenosides.•Young wild-simulated ginseng was up-regulated with response to oxidative stress or abscisic acid. Meanwhile, older wild-simulated ginseng was up-regulated with defense response to bacterium and jasmonic acid.•UGTs known to mediate ginsenoside biosynthesis such as PgUGT74AE2, PgUGT94Q2 and PgUGT100 was highly up-regulated in root tissues of older wild-simulated ginseng.•Putative transcription regulators such as MYB12, ANAC087, MYB111, NAC021, WRKY40, and NAC100 were associated with the expression level of ginsenoside biosynthetic genes.
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Quantitative real-time PCR revealed that jasmonic acid responsive genes, ERDL6, and some UGTs were up-regulated in 13-yr-old ginseng in higher order lateral root tissues. These data suggest that bacterial stimulation in mountain region can enhance the expression of several genes which might support minor ginsenoside biosynthesis. [Display omitted] •Older wild-simulated ginseng had higher amount of ginsenosides.•Young wild-simulated ginseng was up-regulated with response to oxidative stress or abscisic acid. 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Ginseng plants grown in mountain have longer longevity which ensures higher accumulation of ginsenoside components than those grown in farms. However, wild-simulated ginseng over certain age cannot be easily distinguished in morphology. To identify transcriptomic mechanism of ginsenoside accumulation in older wild-simulated ginseng without large phenotype change, we performed comparative transcriptome analysis for leaf, shoot, and root tissues of 7-yr-old and 13yr-old wild-simulated ginseng. Of 559 differentially expressed genes (DEGs) in comparison between 7-yr-old and 13yr-old wild-simulated ginseng, 280 leaf-, 103 shoot-, and 164 root-mainly expressing genes were found to be changed in transcript level according to age. Functional analysis revealed that pentose-phosphate shunt and abscisic acid responsive genes were up-regulated in leaf tissues of 7-yr-old ginseng while defense responsive genes were up-regulated in root tissues of 13-yr-old ginseng. Quantitative real-time PCR revealed that jasmonic acid responsive genes, ERDL6, and some UGTs were up-regulated in 13-yr-old ginseng in higher order lateral root tissues. These data suggest that bacterial stimulation in mountain region can enhance the expression of several genes which might support minor ginsenoside biosynthesis. [Display omitted] •Older wild-simulated ginseng had higher amount of ginsenosides.•Young wild-simulated ginseng was up-regulated with response to oxidative stress or abscisic acid. Meanwhile, older wild-simulated ginseng was up-regulated with defense response to bacterium and jasmonic acid.•UGTs known to mediate ginsenoside biosynthesis such as PgUGT74AE2, PgUGT94Q2 and PgUGT100 was highly up-regulated in root tissues of older wild-simulated ginseng.•Putative transcription regulators such as MYB12, ANAC087, MYB111, NAC021, WRKY40, and NAC100 were associated with the expression level of ginsenoside biosynthetic genes.</description><subject>Ginsenoside biosynthesis</subject><subject>Panax ginseng C.A. meyer</subject><subject>Transcriptome analysis</subject><subject>Wild-simulated ginseng</subject><issn>0981-9428</issn><issn>1873-2690</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNp9kc2O1DAQhC3Eih12eQOEfOSSwT-Z2LkgodHyI63EZfdsdezOrEeOHexk0DwA702GLBw5tdT6qkrdRchbzrac8ebDcTsGGJ_OW8GEXFZKK_aCbLhWshJNy16SDWs1r9pa6GvyupQjY0zUSr4i11LVtWA7tiG_9mkYIcPkT0inDLHY7McpDUghQjgXX2iK9KcPrip-mANM6OjBx4LxQFNPne97zBgnCgekGU8IYSHGVIrvAtIB7RNEX4YLvOpS8W6xt3b-4-dTvCVXPYSCb57nDXn8fPew_1rdf__ybf_pvrKS86lqG2y5hKZR2IJttWwdVxJ75gB2PQfBXKdayzulAQRXHagOdbNzVqoegcsb8n71HXP6MWOZzOCLxRAgYpqLEVpqUXO-axa0XlGbl1My9mbMfoB8NpyZSwHmaNYCzKUAsxawyN49J8zdgO6f6O_HF-DjCuBy58ljNsV6jBadz2gn45L_f8JvCMidNQ</recordid><startdate>20230801</startdate><enddate>20230801</enddate><creator>Kim, Jaewook</creator><creator>Yun, Yeongbae</creator><creator>Huh, Jeonghoon</creator><creator>Um, Yurry</creator><creator>Shim, Donghwan</creator><general>Elsevier Masson SAS</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-3169-2746</orcidid><orcidid>https://orcid.org/0000-0002-0223-595X</orcidid><orcidid>https://orcid.org/0000-0002-0887-9537</orcidid></search><sort><creationdate>20230801</creationdate><title>Comparative transcriptome analysis on wild-simulated ginseng of different age revealed possible mechanism of ginsenoside accumulation</title><author>Kim, Jaewook ; Yun, Yeongbae ; Huh, Jeonghoon ; Um, Yurry ; Shim, Donghwan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c311t-96e913a667e9ac9839d173ef0daa5f1a20db79c1b78aa217ba7be865dc37fea13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Ginsenoside biosynthesis</topic><topic>Panax ginseng C.A. meyer</topic><topic>Transcriptome analysis</topic><topic>Wild-simulated ginseng</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Jaewook</creatorcontrib><creatorcontrib>Yun, Yeongbae</creatorcontrib><creatorcontrib>Huh, Jeonghoon</creatorcontrib><creatorcontrib>Um, Yurry</creatorcontrib><creatorcontrib>Shim, Donghwan</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Plant physiology and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Jaewook</au><au>Yun, Yeongbae</au><au>Huh, Jeonghoon</au><au>Um, Yurry</au><au>Shim, Donghwan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative transcriptome analysis on wild-simulated ginseng of different age revealed possible mechanism of ginsenoside accumulation</atitle><jtitle>Plant physiology and biochemistry</jtitle><addtitle>Plant Physiol Biochem</addtitle><date>2023-08-01</date><risdate>2023</risdate><volume>201</volume><spage>107870</spage><epage>107870</epage><pages>107870-107870</pages><artnum>107870</artnum><issn>0981-9428</issn><eissn>1873-2690</eissn><abstract>Panax ginseng is one of the most famous pharmaceutical plants in Asia. 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Quantitative real-time PCR revealed that jasmonic acid responsive genes, ERDL6, and some UGTs were up-regulated in 13-yr-old ginseng in higher order lateral root tissues. These data suggest that bacterial stimulation in mountain region can enhance the expression of several genes which might support minor ginsenoside biosynthesis. [Display omitted] •Older wild-simulated ginseng had higher amount of ginsenosides.•Young wild-simulated ginseng was up-regulated with response to oxidative stress or abscisic acid. 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source ScienceDirect Journals (5 years ago - present)
subjects Ginsenoside biosynthesis
Panax ginseng C.A. meyer
Transcriptome analysis
Wild-simulated ginseng
title Comparative transcriptome analysis on wild-simulated ginseng of different age revealed possible mechanism of ginsenoside accumulation
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