CsTPS21 encodes a jasmonate-responsive monoterpene synthase producing β-ocimene in citrus against Asian citrus psyllid

Huanglongbing (HLB), spread by the Asian citrus psyllid (ACP), is a widespread, devastating disease that causes significant losses in citrus production. Therefore, controlling the ACP infestation and HLB infection is very important for citrus production. The aim of our study was to identify any citrus volatile which could be used as a repellent or less attractant towards ACP, and to envisage the potential of this strategy to control HLB spread. The present study identified a terpene synthase (TPS)-encoding gene CsTPS21 in citrus plants, and this gene was predicted to encode a monoterpene synthase and had an amino acid sequence similar to β-ocimene synthase. CsTPS21 was significantly upregulated by ACP infestation and methyl jasmonic acid (MeJA) treatment but downregulated by salicylic acid (SA). Further heterologous gene expression studies in yeast cells and tobacco plants indicated that the protein catalyzed the formation of β-ocimene, which acted as an ACP repellent. Detailed analysis of tobacco overexpressing CsTPS21 showed that CsTPS21 synthesizing β-ocimene regulated jasmonic acid (JA)-associated pathways by increasing the JA accumulation and inducing the JA biosynthetic gene expression to defend against insect infestation. These findings provide a basis to plan strategies to manage HLB in the field using β-ocimene and CsTPS21 as candidates. •We identified a chloroplast-localized terpene synthase CsTPS21 which was similar to other β-ocimene synthases.•CsTPS21 was significantly upregulated by Asian citrus psyllid (ACP) infestation and methyl jasmonic acid (MeJA) treatment.•Transgenic tobacco overexpressing CsTPS21 produced higher levels of β-ocimene and showed stronger repellency to ACP.•CsTPS21-OE transgenic plants exhibited higher JA accumulation and JA biosynthetic gene expression than the WT.