Comparative physiological, antioxidant and proteomic investigation reveal robust response to cold stress in Digitalis purpurea L
Background of the study Digitalis purpurea (L) is an important medicinal plant growing at Alpine region of Himalayas and withstands low temperatures and harsh climatic conditions existing at high altitude. It serves as an ideal plant system to decipher the tolerance to cold stress (CS) in plants fro...
Gespeichert in:
| Veröffentlicht in: | Molecular biology reports 2023-09, Vol.50 (9), p.7319-7331 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 7331 |
|---|---|
| container_issue | 9 |
| container_start_page | 7319 |
| container_title | Molecular biology reports |
| container_volume | 50 |
| creator | Jan, Nelofer Wani, Umer Majeed Wani, Mubashir Ahmad Qazi, Hilal Ahmad John, Riffat |
| description | Background of the study
Digitalis purpurea
(L) is an important medicinal plant growing at Alpine region of Himalayas and withstands low temperatures and harsh climatic conditions existing at high altitude. It serves as an ideal plant system to decipher the tolerance to cold stress (CS) in plants from high altitudes.
Methods and results
To understand the complexity of plant response to CS, we performed a comparative physiological and biochemical study complemented with proteomics in one-month-old
D. purpurea
grown at 25 °C (control) and 4 °C (CS). We observed an enhanced accumulation of different osmo-protectants (glycine betaine, soluble sugar and proline) and higher transcription (mRNA levels) of various antioxidant enzymes with an increased antioxidant enzyme activity in
D. purpurea
when exposed to CS. Furthermore, higher concentrations of non-enzymatic antioxidants (flavonoids, phenolics) was also associated with the response to CS. Differential proteomic analysis revealed the role of various proteins primarily involved in redox reactions, protein stabilization, quinone and sterol metabolism involved in CS response in
D. purpurea
..
Conclusion
Our results provide a framework for better understanding the physiological and molecular mechanism of CS response in
D. purpurea
at high altitudes. |
| doi_str_mv | 10.1007/s11033-023-08635-7 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2836874700</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2857701942</sourcerecordid><originalsourceid>FETCH-LOGICAL-c375t-f141803be472b6190f86e911c129576ad3984d431fb3a7ecf7243467825392fc3</originalsourceid><addsrcrecordid>eNp9kU1v1DAQhq0K1G4Lf4ADssSFAwF_JeMcqwVKpZW4wNlyHGdxlcSp7azojZ_OwBYq9VDJ1ozt5x3P6CXkFWfvOWPwIXPOpKyYwK0bWVdwQja8BlmpFvQzsmGS8Urpmp-R85xvGGOKQ31KziQo2epWb8ivbZwWm2wJB0-XH3c5xDHug7PjO2rnEuLP0GPEvKdLisXHKTga5oPPJexRFmea_MHbkabYrbngKS9xzp6WSF0ce5oLXmXU0I9hH4odQ6bLmnB5S3cvyPPBjtm_vI8X5PvnT9-2X6rd16vr7eWuchLqUg1ccc1k5xWIruEtG3TjW84dF20Nje1xHtUryYdOWvBuAKGkakCLWrZicPKCvD3WxSluV-zeTCE7P4529nHNRmjZaFDAGKJvHqE3cU0zdodUDcB4qwRS4ki5FHNOfjBLCpNNd4Yz88cfc_THoD_mrz8GUPT6vvTaTb7_L_lnCALyCGR8mvc-Pfz9RNnfAlKcgQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2857701942</pqid></control><display><type>article</type><title>Comparative physiological, antioxidant and proteomic investigation reveal robust response to cold stress in Digitalis purpurea L</title><source>SpringerNature Journals</source><creator>Jan, Nelofer ; Wani, Umer Majeed ; Wani, Mubashir Ahmad ; Qazi, Hilal Ahmad ; John, Riffat</creator><creatorcontrib>Jan, Nelofer ; Wani, Umer Majeed ; Wani, Mubashir Ahmad ; Qazi, Hilal Ahmad ; John, Riffat</creatorcontrib><description>Background of the study
Digitalis purpurea
(L) is an important medicinal plant growing at Alpine region of Himalayas and withstands low temperatures and harsh climatic conditions existing at high altitude. It serves as an ideal plant system to decipher the tolerance to cold stress (CS) in plants from high altitudes.
Methods and results
To understand the complexity of plant response to CS, we performed a comparative physiological and biochemical study complemented with proteomics in one-month-old
D. purpurea
grown at 25 °C (control) and 4 °C (CS). We observed an enhanced accumulation of different osmo-protectants (glycine betaine, soluble sugar and proline) and higher transcription (mRNA levels) of various antioxidant enzymes with an increased antioxidant enzyme activity in
D. purpurea
when exposed to CS. Furthermore, higher concentrations of non-enzymatic antioxidants (flavonoids, phenolics) was also associated with the response to CS. Differential proteomic analysis revealed the role of various proteins primarily involved in redox reactions, protein stabilization, quinone and sterol metabolism involved in CS response in
D. purpurea
..
Conclusion
Our results provide a framework for better understanding the physiological and molecular mechanism of CS response in
D. purpurea
at high altitudes.</description><identifier>ISSN: 0301-4851</identifier><identifier>EISSN: 1573-4978</identifier><identifier>DOI: 10.1007/s11033-023-08635-7</identifier><identifier>PMID: 37439898</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Altitude ; Animal Anatomy ; Animal Biochemistry ; Antioxidants ; Biomedical and Life Sciences ; Climatic conditions ; Cold tolerance ; Digitalis purpurea ; Enzymatic activity ; Flavonoids ; Glycine betaine ; High-altitude environments ; Histology ; Life Sciences ; Low temperature ; Medicinal plants ; Molecular modelling ; Morphology ; Original Article ; Physiology ; Protein turnover ; Proteomics ; Redox reactions</subject><ispartof>Molecular biology reports, 2023-09, Vol.50 (9), p.7319-7331</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2023. The Author(s), under exclusive licence to Springer Nature B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-f141803be472b6190f86e911c129576ad3984d431fb3a7ecf7243467825392fc3</citedby><cites>FETCH-LOGICAL-c375t-f141803be472b6190f86e911c129576ad3984d431fb3a7ecf7243467825392fc3</cites><orcidid>0000-0001-6258-9381</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11033-023-08635-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11033-023-08635-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37439898$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jan, Nelofer</creatorcontrib><creatorcontrib>Wani, Umer Majeed</creatorcontrib><creatorcontrib>Wani, Mubashir Ahmad</creatorcontrib><creatorcontrib>Qazi, Hilal Ahmad</creatorcontrib><creatorcontrib>John, Riffat</creatorcontrib><title>Comparative physiological, antioxidant and proteomic investigation reveal robust response to cold stress in Digitalis purpurea L</title><title>Molecular biology reports</title><addtitle>Mol Biol Rep</addtitle><addtitle>Mol Biol Rep</addtitle><description>Background of the study
Digitalis purpurea
(L) is an important medicinal plant growing at Alpine region of Himalayas and withstands low temperatures and harsh climatic conditions existing at high altitude. It serves as an ideal plant system to decipher the tolerance to cold stress (CS) in plants from high altitudes.
Methods and results
To understand the complexity of plant response to CS, we performed a comparative physiological and biochemical study complemented with proteomics in one-month-old
D. purpurea
grown at 25 °C (control) and 4 °C (CS). We observed an enhanced accumulation of different osmo-protectants (glycine betaine, soluble sugar and proline) and higher transcription (mRNA levels) of various antioxidant enzymes with an increased antioxidant enzyme activity in
D. purpurea
when exposed to CS. Furthermore, higher concentrations of non-enzymatic antioxidants (flavonoids, phenolics) was also associated with the response to CS. Differential proteomic analysis revealed the role of various proteins primarily involved in redox reactions, protein stabilization, quinone and sterol metabolism involved in CS response in
D. purpurea
..
Conclusion
Our results provide a framework for better understanding the physiological and molecular mechanism of CS response in
D. purpurea
at high altitudes.</description><subject>Altitude</subject><subject>Animal Anatomy</subject><subject>Animal Biochemistry</subject><subject>Antioxidants</subject><subject>Biomedical and Life Sciences</subject><subject>Climatic conditions</subject><subject>Cold tolerance</subject><subject>Digitalis purpurea</subject><subject>Enzymatic activity</subject><subject>Flavonoids</subject><subject>Glycine betaine</subject><subject>High-altitude environments</subject><subject>Histology</subject><subject>Life Sciences</subject><subject>Low temperature</subject><subject>Medicinal plants</subject><subject>Molecular modelling</subject><subject>Morphology</subject><subject>Original Article</subject><subject>Physiology</subject><subject>Protein turnover</subject><subject>Proteomics</subject><subject>Redox reactions</subject><issn>0301-4851</issn><issn>1573-4978</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kU1v1DAQhq0K1G4Lf4ADssSFAwF_JeMcqwVKpZW4wNlyHGdxlcSp7azojZ_OwBYq9VDJ1ozt5x3P6CXkFWfvOWPwIXPOpKyYwK0bWVdwQja8BlmpFvQzsmGS8Urpmp-R85xvGGOKQ31KziQo2epWb8ivbZwWm2wJB0-XH3c5xDHug7PjO2rnEuLP0GPEvKdLisXHKTga5oPPJexRFmea_MHbkabYrbngKS9xzp6WSF0ce5oLXmXU0I9hH4odQ6bLmnB5S3cvyPPBjtm_vI8X5PvnT9-2X6rd16vr7eWuchLqUg1ccc1k5xWIruEtG3TjW84dF20Nje1xHtUryYdOWvBuAKGkakCLWrZicPKCvD3WxSluV-zeTCE7P4529nHNRmjZaFDAGKJvHqE3cU0zdodUDcB4qwRS4ki5FHNOfjBLCpNNd4Yz88cfc_THoD_mrz8GUPT6vvTaTb7_L_lnCALyCGR8mvc-Pfz9RNnfAlKcgQ</recordid><startdate>20230901</startdate><enddate>20230901</enddate><creator>Jan, Nelofer</creator><creator>Wani, Umer Majeed</creator><creator>Wani, Mubashir Ahmad</creator><creator>Qazi, Hilal Ahmad</creator><creator>John, Riffat</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6258-9381</orcidid></search><sort><creationdate>20230901</creationdate><title>Comparative physiological, antioxidant and proteomic investigation reveal robust response to cold stress in Digitalis purpurea L</title><author>Jan, Nelofer ; Wani, Umer Majeed ; Wani, Mubashir Ahmad ; Qazi, Hilal Ahmad ; John, Riffat</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-f141803be472b6190f86e911c129576ad3984d431fb3a7ecf7243467825392fc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Altitude</topic><topic>Animal Anatomy</topic><topic>Animal Biochemistry</topic><topic>Antioxidants</topic><topic>Biomedical and Life Sciences</topic><topic>Climatic conditions</topic><topic>Cold tolerance</topic><topic>Digitalis purpurea</topic><topic>Enzymatic activity</topic><topic>Flavonoids</topic><topic>Glycine betaine</topic><topic>High-altitude environments</topic><topic>Histology</topic><topic>Life Sciences</topic><topic>Low temperature</topic><topic>Medicinal plants</topic><topic>Molecular modelling</topic><topic>Morphology</topic><topic>Original Article</topic><topic>Physiology</topic><topic>Protein turnover</topic><topic>Proteomics</topic><topic>Redox reactions</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jan, Nelofer</creatorcontrib><creatorcontrib>Wani, Umer Majeed</creatorcontrib><creatorcontrib>Wani, Mubashir Ahmad</creatorcontrib><creatorcontrib>Qazi, Hilal Ahmad</creatorcontrib><creatorcontrib>John, Riffat</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular biology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jan, Nelofer</au><au>Wani, Umer Majeed</au><au>Wani, Mubashir Ahmad</au><au>Qazi, Hilal Ahmad</au><au>John, Riffat</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative physiological, antioxidant and proteomic investigation reveal robust response to cold stress in Digitalis purpurea L</atitle><jtitle>Molecular biology reports</jtitle><stitle>Mol Biol Rep</stitle><addtitle>Mol Biol Rep</addtitle><date>2023-09-01</date><risdate>2023</risdate><volume>50</volume><issue>9</issue><spage>7319</spage><epage>7331</epage><pages>7319-7331</pages><issn>0301-4851</issn><eissn>1573-4978</eissn><abstract>Background of the study
Digitalis purpurea
(L) is an important medicinal plant growing at Alpine region of Himalayas and withstands low temperatures and harsh climatic conditions existing at high altitude. It serves as an ideal plant system to decipher the tolerance to cold stress (CS) in plants from high altitudes.
Methods and results
To understand the complexity of plant response to CS, we performed a comparative physiological and biochemical study complemented with proteomics in one-month-old
D. purpurea
grown at 25 °C (control) and 4 °C (CS). We observed an enhanced accumulation of different osmo-protectants (glycine betaine, soluble sugar and proline) and higher transcription (mRNA levels) of various antioxidant enzymes with an increased antioxidant enzyme activity in
D. purpurea
when exposed to CS. Furthermore, higher concentrations of non-enzymatic antioxidants (flavonoids, phenolics) was also associated with the response to CS. Differential proteomic analysis revealed the role of various proteins primarily involved in redox reactions, protein stabilization, quinone and sterol metabolism involved in CS response in
D. purpurea
..
Conclusion
Our results provide a framework for better understanding the physiological and molecular mechanism of CS response in
D. purpurea
at high altitudes.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>37439898</pmid><doi>10.1007/s11033-023-08635-7</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0001-6258-9381</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0301-4851 |
| ispartof | Molecular biology reports, 2023-09, Vol.50 (9), p.7319-7331 |
| issn | 0301-4851 1573-4978 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2836874700 |
| source | SpringerNature Journals |
| subjects | Altitude Animal Anatomy Animal Biochemistry Antioxidants Biomedical and Life Sciences Climatic conditions Cold tolerance Digitalis purpurea Enzymatic activity Flavonoids Glycine betaine High-altitude environments Histology Life Sciences Low temperature Medicinal plants Molecular modelling Morphology Original Article Physiology Protein turnover Proteomics Redox reactions |
| title | Comparative physiological, antioxidant and proteomic investigation reveal robust response to cold stress in Digitalis purpurea L |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-22T08%3A52%3A05IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Comparative%20physiological,%20antioxidant%20and%20proteomic%20investigation%20reveal%20robust%20response%20to%20cold%20stress%20in%20Digitalis%20purpurea%20L&rft.jtitle=Molecular%20biology%20reports&rft.au=Jan,%20Nelofer&rft.date=2023-09-01&rft.volume=50&rft.issue=9&rft.spage=7319&rft.epage=7331&rft.pages=7319-7331&rft.issn=0301-4851&rft.eissn=1573-4978&rft_id=info:doi/10.1007/s11033-023-08635-7&rft_dat=%3Cproquest_cross%3E2857701942%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2857701942&rft_id=info:pmid/37439898&rfr_iscdi=true |