Integrated metabolome and transcriptome analysis of anthocyanin accumulation during the color formation of bicolor flowers in Eustoma grandiflorum
•7 anthocyanins related to the bicolor flower formation of E. grandiflorum were identified.•The key structural genes and TFs related to flavonoid biosynthesis were screened.•EgCHS1 showed significantly higher expression in the upper tepals than in the tepal bases. The bicolor flowers of Eustoma gran...
Gespeichert in:
| Veröffentlicht in: | Scientia horticulturae 2023-04, Vol.314, p.111952, Article 111952 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | 111952 |
| container_title | Scientia horticulturae |
| container_volume | 314 |
| creator | Fan, Yong Sun, Liang Song, Shengli Sun, Yue Fan, Xinyue Li, Yuefei Li, Ruichun Sun, Hongmei |
| description | •7 anthocyanins related to the bicolor flower formation of E. grandiflorum were identified.•The key structural genes and TFs related to flavonoid biosynthesis were screened.•EgCHS1 showed significantly higher expression in the upper tepals than in the tepal bases.
The bicolor flowers of Eustoma grandiflorum have a special phenotype and high ornamental value. However, the coloration mechanism of these flowers has not been reported. We used metabolomics and transcriptomics to analyze the accumulation of secondary metabolites and the expression of differential genes in bicolor petals at different developmental stages, to elucidate the molecular mechanism underlying anthocyanin accumulation in these bicolor flowers of E. grandiflorum. The metabolomics analysis revealed that five kinds of anthocyanins in the upper pigmented petals at different developmental stages, and three kinds of anthocyanins were identified in the comparison between the pink upper tepals and the white bases at stage 3, with pelargonidin 3-glucoside 5-(6-coumaroyl) glucoside showing the most significant difference. The differentially expressed genes (DEGs) related to flavonoid biosynthesis in bicolor flowers were further analyzed by transcriptomics, and 25 structural genes and 5 MYB transcription factors (TFs) were screened, among which EgCHS1 showed significantly higher expression (approximately 21.5 times) in the upper tepals than in the tepal bases. Transcriptomic and metabolomic association analysis showed that the EgFLS2 and EgFLS3 genes were related to the largest quantity differentially accumulated metabolites (DAMs), and ferulate was associated with the largest quantity of DEGs. This study provides a basis for anthocyanin accumulation and coloration mechanism during the development of E. grandiflorum bicolor flowers. The results will be conducive for promoting artificial regulation of flower color and breeding new cultivars of E. grandiflorum. |
| doi_str_mv | 10.1016/j.scienta.2023.111952 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2834277608</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0304423823001267</els_id><sourcerecordid>2834277608</sourcerecordid><originalsourceid>FETCH-LOGICAL-c342t-184397b5acaa7501136c2efe7c2fb2785dce2fc1b2cc3ab10ac9eb04eb16521d3</originalsourceid><addsrcrecordid>eNqFUUtLxDAQDqLg-vgJQo5euiZpu21PIuILBC96DtPp1M3SJmuSKvs3_MVm6d49zTDfY2b4GLuSYimFXN1slgEN2QhLJVS-lFI2pTpiC1lXTZYY9TFbiFwUWaHy-pSdhbARQkhZNAv2-2IjfXqI1PGRIrRucCNxsB2PHmxAb7ZxnsCwCyZw16c-rh3uwBrLAXEapwGicZZ3kzf2k8c1cUxGnvfOjzOUZK05DAf3Qz7wpH6YQnIHni6wnUmAn8YLdtLDEOjyUM_Zx-PD-_1z9vr29HJ_95phXqiYybrIm6otAQGqMr2Tr1BRTxWqvlVVXXZIqkfZKsQcWikAG2pFQa1clUp2-Tm7nn233n1NFKIeTUAaBrDkpqBVnfZU1UrUiVrOVPQuBE-93nozgt9pKfQ-A73Rhwz0PgM9Z5B0t7OO0h_fhvzMQuqMJ4y6c-Yfhz_hvpfH</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2834277608</pqid></control><display><type>article</type><title>Integrated metabolome and transcriptome analysis of anthocyanin accumulation during the color formation of bicolor flowers in Eustoma grandiflorum</title><source>Elsevier ScienceDirect Journals</source><creator>Fan, Yong ; Sun, Liang ; Song, Shengli ; Sun, Yue ; Fan, Xinyue ; Li, Yuefei ; Li, Ruichun ; Sun, Hongmei</creator><creatorcontrib>Fan, Yong ; Sun, Liang ; Song, Shengli ; Sun, Yue ; Fan, Xinyue ; Li, Yuefei ; Li, Ruichun ; Sun, Hongmei</creatorcontrib><description>•7 anthocyanins related to the bicolor flower formation of E. grandiflorum were identified.•The key structural genes and TFs related to flavonoid biosynthesis were screened.•EgCHS1 showed significantly higher expression in the upper tepals than in the tepal bases.
The bicolor flowers of Eustoma grandiflorum have a special phenotype and high ornamental value. However, the coloration mechanism of these flowers has not been reported. We used metabolomics and transcriptomics to analyze the accumulation of secondary metabolites and the expression of differential genes in bicolor petals at different developmental stages, to elucidate the molecular mechanism underlying anthocyanin accumulation in these bicolor flowers of E. grandiflorum. The metabolomics analysis revealed that five kinds of anthocyanins in the upper pigmented petals at different developmental stages, and three kinds of anthocyanins were identified in the comparison between the pink upper tepals and the white bases at stage 3, with pelargonidin 3-glucoside 5-(6-coumaroyl) glucoside showing the most significant difference. The differentially expressed genes (DEGs) related to flavonoid biosynthesis in bicolor flowers were further analyzed by transcriptomics, and 25 structural genes and 5 MYB transcription factors (TFs) were screened, among which EgCHS1 showed significantly higher expression (approximately 21.5 times) in the upper tepals than in the tepal bases. Transcriptomic and metabolomic association analysis showed that the EgFLS2 and EgFLS3 genes were related to the largest quantity differentially accumulated metabolites (DAMs), and ferulate was associated with the largest quantity of DEGs. This study provides a basis for anthocyanin accumulation and coloration mechanism during the development of E. grandiflorum bicolor flowers. The results will be conducive for promoting artificial regulation of flower color and breeding new cultivars of E. grandiflorum.</description><identifier>ISSN: 0304-4238</identifier><identifier>EISSN: 1879-1018</identifier><identifier>DOI: 10.1016/j.scienta.2023.111952</identifier><language>eng</language><publisher>Elsevier B.V</publisher><subject>Anthocyanin biosynthesis pathway ; Bicolor flower ; biosynthesis ; callistephin ; color ; cultivars ; Eustoma exaltatum subsp. russellianum ; Eustoma grandiflorum ; flower color ; flower tepals ; gene expression regulation ; glucosides ; Metabolome ; metabolomics ; ornamental value ; phenotype ; secondary metabolites ; Transcriptome ; transcriptomics</subject><ispartof>Scientia horticulturae, 2023-04, Vol.314, p.111952, Article 111952</ispartof><rights>2023</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c342t-184397b5acaa7501136c2efe7c2fb2785dce2fc1b2cc3ab10ac9eb04eb16521d3</citedby><cites>FETCH-LOGICAL-c342t-184397b5acaa7501136c2efe7c2fb2785dce2fc1b2cc3ab10ac9eb04eb16521d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0304423823001267$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids></links><search><creatorcontrib>Fan, Yong</creatorcontrib><creatorcontrib>Sun, Liang</creatorcontrib><creatorcontrib>Song, Shengli</creatorcontrib><creatorcontrib>Sun, Yue</creatorcontrib><creatorcontrib>Fan, Xinyue</creatorcontrib><creatorcontrib>Li, Yuefei</creatorcontrib><creatorcontrib>Li, Ruichun</creatorcontrib><creatorcontrib>Sun, Hongmei</creatorcontrib><title>Integrated metabolome and transcriptome analysis of anthocyanin accumulation during the color formation of bicolor flowers in Eustoma grandiflorum</title><title>Scientia horticulturae</title><description>•7 anthocyanins related to the bicolor flower formation of E. grandiflorum were identified.•The key structural genes and TFs related to flavonoid biosynthesis were screened.•EgCHS1 showed significantly higher expression in the upper tepals than in the tepal bases.
The bicolor flowers of Eustoma grandiflorum have a special phenotype and high ornamental value. However, the coloration mechanism of these flowers has not been reported. We used metabolomics and transcriptomics to analyze the accumulation of secondary metabolites and the expression of differential genes in bicolor petals at different developmental stages, to elucidate the molecular mechanism underlying anthocyanin accumulation in these bicolor flowers of E. grandiflorum. The metabolomics analysis revealed that five kinds of anthocyanins in the upper pigmented petals at different developmental stages, and three kinds of anthocyanins were identified in the comparison between the pink upper tepals and the white bases at stage 3, with pelargonidin 3-glucoside 5-(6-coumaroyl) glucoside showing the most significant difference. The differentially expressed genes (DEGs) related to flavonoid biosynthesis in bicolor flowers were further analyzed by transcriptomics, and 25 structural genes and 5 MYB transcription factors (TFs) were screened, among which EgCHS1 showed significantly higher expression (approximately 21.5 times) in the upper tepals than in the tepal bases. Transcriptomic and metabolomic association analysis showed that the EgFLS2 and EgFLS3 genes were related to the largest quantity differentially accumulated metabolites (DAMs), and ferulate was associated with the largest quantity of DEGs. This study provides a basis for anthocyanin accumulation and coloration mechanism during the development of E. grandiflorum bicolor flowers. The results will be conducive for promoting artificial regulation of flower color and breeding new cultivars of E. grandiflorum.</description><subject>Anthocyanin biosynthesis pathway</subject><subject>Bicolor flower</subject><subject>biosynthesis</subject><subject>callistephin</subject><subject>color</subject><subject>cultivars</subject><subject>Eustoma exaltatum subsp. russellianum</subject><subject>Eustoma grandiflorum</subject><subject>flower color</subject><subject>flower tepals</subject><subject>gene expression regulation</subject><subject>glucosides</subject><subject>Metabolome</subject><subject>metabolomics</subject><subject>ornamental value</subject><subject>phenotype</subject><subject>secondary metabolites</subject><subject>Transcriptome</subject><subject>transcriptomics</subject><issn>0304-4238</issn><issn>1879-1018</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNqFUUtLxDAQDqLg-vgJQo5euiZpu21PIuILBC96DtPp1M3SJmuSKvs3_MVm6d49zTDfY2b4GLuSYimFXN1slgEN2QhLJVS-lFI2pTpiC1lXTZYY9TFbiFwUWaHy-pSdhbARQkhZNAv2-2IjfXqI1PGRIrRucCNxsB2PHmxAb7ZxnsCwCyZw16c-rh3uwBrLAXEapwGicZZ3kzf2k8c1cUxGnvfOjzOUZK05DAf3Qz7wpH6YQnIHni6wnUmAn8YLdtLDEOjyUM_Zx-PD-_1z9vr29HJ_95phXqiYybrIm6otAQGqMr2Tr1BRTxWqvlVVXXZIqkfZKsQcWikAG2pFQa1clUp2-Tm7nn233n1NFKIeTUAaBrDkpqBVnfZU1UrUiVrOVPQuBE-93nozgt9pKfQ-A73Rhwz0PgM9Z5B0t7OO0h_fhvzMQuqMJ4y6c-Yfhz_hvpfH</recordid><startdate>20230415</startdate><enddate>20230415</enddate><creator>Fan, Yong</creator><creator>Sun, Liang</creator><creator>Song, Shengli</creator><creator>Sun, Yue</creator><creator>Fan, Xinyue</creator><creator>Li, Yuefei</creator><creator>Li, Ruichun</creator><creator>Sun, Hongmei</creator><general>Elsevier B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20230415</creationdate><title>Integrated metabolome and transcriptome analysis of anthocyanin accumulation during the color formation of bicolor flowers in Eustoma grandiflorum</title><author>Fan, Yong ; Sun, Liang ; Song, Shengli ; Sun, Yue ; Fan, Xinyue ; Li, Yuefei ; Li, Ruichun ; Sun, Hongmei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c342t-184397b5acaa7501136c2efe7c2fb2785dce2fc1b2cc3ab10ac9eb04eb16521d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Anthocyanin biosynthesis pathway</topic><topic>Bicolor flower</topic><topic>biosynthesis</topic><topic>callistephin</topic><topic>color</topic><topic>cultivars</topic><topic>Eustoma exaltatum subsp. russellianum</topic><topic>Eustoma grandiflorum</topic><topic>flower color</topic><topic>flower tepals</topic><topic>gene expression regulation</topic><topic>glucosides</topic><topic>Metabolome</topic><topic>metabolomics</topic><topic>ornamental value</topic><topic>phenotype</topic><topic>secondary metabolites</topic><topic>Transcriptome</topic><topic>transcriptomics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fan, Yong</creatorcontrib><creatorcontrib>Sun, Liang</creatorcontrib><creatorcontrib>Song, Shengli</creatorcontrib><creatorcontrib>Sun, Yue</creatorcontrib><creatorcontrib>Fan, Xinyue</creatorcontrib><creatorcontrib>Li, Yuefei</creatorcontrib><creatorcontrib>Li, Ruichun</creatorcontrib><creatorcontrib>Sun, Hongmei</creatorcontrib><collection>CrossRef</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Scientia horticulturae</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fan, Yong</au><au>Sun, Liang</au><au>Song, Shengli</au><au>Sun, Yue</au><au>Fan, Xinyue</au><au>Li, Yuefei</au><au>Li, Ruichun</au><au>Sun, Hongmei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Integrated metabolome and transcriptome analysis of anthocyanin accumulation during the color formation of bicolor flowers in Eustoma grandiflorum</atitle><jtitle>Scientia horticulturae</jtitle><date>2023-04-15</date><risdate>2023</risdate><volume>314</volume><spage>111952</spage><pages>111952-</pages><artnum>111952</artnum><issn>0304-4238</issn><eissn>1879-1018</eissn><abstract>•7 anthocyanins related to the bicolor flower formation of E. grandiflorum were identified.•The key structural genes and TFs related to flavonoid biosynthesis were screened.•EgCHS1 showed significantly higher expression in the upper tepals than in the tepal bases.
The bicolor flowers of Eustoma grandiflorum have a special phenotype and high ornamental value. However, the coloration mechanism of these flowers has not been reported. We used metabolomics and transcriptomics to analyze the accumulation of secondary metabolites and the expression of differential genes in bicolor petals at different developmental stages, to elucidate the molecular mechanism underlying anthocyanin accumulation in these bicolor flowers of E. grandiflorum. The metabolomics analysis revealed that five kinds of anthocyanins in the upper pigmented petals at different developmental stages, and three kinds of anthocyanins were identified in the comparison between the pink upper tepals and the white bases at stage 3, with pelargonidin 3-glucoside 5-(6-coumaroyl) glucoside showing the most significant difference. The differentially expressed genes (DEGs) related to flavonoid biosynthesis in bicolor flowers were further analyzed by transcriptomics, and 25 structural genes and 5 MYB transcription factors (TFs) were screened, among which EgCHS1 showed significantly higher expression (approximately 21.5 times) in the upper tepals than in the tepal bases. Transcriptomic and metabolomic association analysis showed that the EgFLS2 and EgFLS3 genes were related to the largest quantity differentially accumulated metabolites (DAMs), and ferulate was associated with the largest quantity of DEGs. This study provides a basis for anthocyanin accumulation and coloration mechanism during the development of E. grandiflorum bicolor flowers. The results will be conducive for promoting artificial regulation of flower color and breeding new cultivars of E. grandiflorum.</abstract><pub>Elsevier B.V</pub><doi>10.1016/j.scienta.2023.111952</doi></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0304-4238 |
| ispartof | Scientia horticulturae, 2023-04, Vol.314, p.111952, Article 111952 |
| issn | 0304-4238 1879-1018 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2834277608 |
| source | Elsevier ScienceDirect Journals |
| subjects | Anthocyanin biosynthesis pathway Bicolor flower biosynthesis callistephin color cultivars Eustoma exaltatum subsp. russellianum Eustoma grandiflorum flower color flower tepals gene expression regulation glucosides Metabolome metabolomics ornamental value phenotype secondary metabolites Transcriptome transcriptomics |
| title | Integrated metabolome and transcriptome analysis of anthocyanin accumulation during the color formation of bicolor flowers in Eustoma grandiflorum |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-30T07%3A48%3A53IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Integrated%20metabolome%20and%20transcriptome%20analysis%20of%20anthocyanin%20accumulation%20during%20the%20color%20formation%20of%20bicolor%20flowers%20in%20Eustoma%20grandiflorum&rft.jtitle=Scientia%20horticulturae&rft.au=Fan,%20Yong&rft.date=2023-04-15&rft.volume=314&rft.spage=111952&rft.pages=111952-&rft.artnum=111952&rft.issn=0304-4238&rft.eissn=1879-1018&rft_id=info:doi/10.1016/j.scienta.2023.111952&rft_dat=%3Cproquest_cross%3E2834277608%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2834277608&rft_id=info:pmid/&rft_els_id=S0304423823001267&rfr_iscdi=true |