Attenuative effect of astilbin on polystyrene microplastics induced testicular damage: Biochemical, spermatological and histopathological-based evidences
Polystyrene microplastics (PS-MPs) are the potential environmental pollutants that possess the ability to induce testicular damage. Astilbin (ASB) is a dihydroflavonol, abundantly reported in multiple plants that has various pharmacological properties. This research elucidated the mitigative potenti...
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| Veröffentlicht in: | Toxicology and applied pharmacology 2023-07, Vol.471, p.116559-116559, Article 116559 |
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| description | Polystyrene microplastics (PS-MPs) are the potential environmental pollutants that possess the ability to induce testicular damage. Astilbin (ASB) is a dihydroflavonol, abundantly reported in multiple plants that has various pharmacological properties. This research elucidated the mitigative potential of ASB against PS-MPs-instigated testicular toxicity. 48 adult male rats (200 ± 10 g) were distributed into 4 groups (n = 12): control, PS-MPs received (0.01 mg/kg), PS-MPs + ASB received (0.01 mg/kg + 20 mg/kg) and ASB supplemented group (20 mg/kg). After 56th day of the trial, animals were sacrificed and testes were harvested for the estimation of biochemical, hormonal, spermatogenic, steroidogenic, apoptotic and histological profiles. PS-MPs intoxication significantly (P |
| doi_str_mv | 10.1016/j.taap.2023.116559 |
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[Display omitted]
•PS-MPs exposure damaged biochemical, spermatogenic and histological profiles.•ASB increased antioxidant enzyme activities and decreased MDA and ROS levels.•ASB reversed the levels of inflammatory and apoptotic markers.•Moreover, ASB increased the expression of steroidogenic enzymes and hormone levels.•ASB also improved all the histopathological alterations in the testicular tissues.</description><identifier>ISSN: 0041-008X</identifier><identifier>EISSN: 1096-0333</identifier><identifier>DOI: 10.1016/j.taap.2023.116559</identifier><identifier>PMID: 37217007</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>adults ; Animals ; Antioxidant ; antioxidants ; Antioxidants - pharmacology ; apoptosis ; Astilbin ; caspase-3 ; catalase ; epididymis ; follicle-stimulating hormone ; glutathione peroxidase ; glutathione-disulfide reductase ; histology ; histopathology ; interleukin-6 ; luteinizing hormone ; Male ; males ; microplastics ; Microplastics - metabolism ; Microplastics - pharmacology ; necrosis ; neoplasms ; Oxidative Stress ; pharmacology ; Plastics - metabolism ; poisoning ; Polystyrene Microplastics ; polystyrenes ; Polystyrenes - metabolism ; Polystyrenes - toxicity ; prostaglandin synthase ; Rats ; Rats, Wistar ; Semen - metabolism ; spermatozoa ; superoxide dismutase ; testes ; Testicular Damage ; Testis ; testosterone ; toxicity ; viability</subject><ispartof>Toxicology and applied pharmacology, 2023-07, Vol.471, p.116559-116559, Article 116559</ispartof><rights>2023 Elsevier Inc.</rights><rights>Copyright © 2023 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-e392360458d86904c42254dcfddbd1813663f3abd5991724b301fc2a8c21dfe63</citedby><cites>FETCH-LOGICAL-c389t-e392360458d86904c42254dcfddbd1813663f3abd5991724b301fc2a8c21dfe63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0041008X23001989$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37217007$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rizwan, Arooj</creatorcontrib><creatorcontrib>Ijaz, Muhammad Umar</creatorcontrib><creatorcontrib>Hamza, Ali</creatorcontrib><creatorcontrib>Anwar, Haseeb</creatorcontrib><title>Attenuative effect of astilbin on polystyrene microplastics induced testicular damage: Biochemical, spermatological and histopathological-based evidences</title><title>Toxicology and applied pharmacology</title><addtitle>Toxicol Appl Pharmacol</addtitle><description>Polystyrene microplastics (PS-MPs) are the potential environmental pollutants that possess the ability to induce testicular damage. Astilbin (ASB) is a dihydroflavonol, abundantly reported in multiple plants that has various pharmacological properties. This research elucidated the mitigative potential of ASB against PS-MPs-instigated testicular toxicity. 48 adult male rats (200 ± 10 g) were distributed into 4 groups (n = 12): control, PS-MPs received (0.01 mg/kg), PS-MPs + ASB received (0.01 mg/kg + 20 mg/kg) and ASB supplemented group (20 mg/kg). After 56th day of the trial, animals were sacrificed and testes were harvested for the estimation of biochemical, hormonal, spermatogenic, steroidogenic, apoptotic and histological profiles. PS-MPs intoxication significantly (P < 0.05) lowered glutathione peroxidase (GPx), superoxide dismutase (SOD), glutathione reductase (GSR) as well as catalase (CAT) activities, whereas elevated MDA as well as ROS levels. Besides, the levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), nuclear factor kappa-B (NF-κB) along with cyclooxygenase-2 (COX-2) activity were raised. PS-MPs treatment reduced luteinizing hormone (LH), plasma testosterone and follicle-stimulating hormone (FSH) level besides decreased epididymal sperm number, viability, motility as well as the count of HOS coil-tailed spermatozoa and increased sperm morphological irregularities. PS-MPs exposure lowered steroidogenic enzymes (17β-HSD, 3β-HSD and StAR protein along with Bcl-2 expression, besides increasing Caspase-3 and Bax expressions and histopathological alterations in testicular tissues. However, ASB treatment significantly reversed PS-MPs mediated damage. In conclusion, ASB administration is protective against PS-MPs-instigated testicular damage owing to its anti-inflammatory, anti-apoptotic, antioxidant and androgenic nature.
[Display omitted]
•PS-MPs exposure damaged biochemical, spermatogenic and histological profiles.•ASB increased antioxidant enzyme activities and decreased MDA and ROS levels.•ASB reversed the levels of inflammatory and apoptotic markers.•Moreover, ASB increased the expression of steroidogenic enzymes and hormone levels.•ASB also improved all the histopathological alterations in the testicular tissues.</description><subject>adults</subject><subject>Animals</subject><subject>Antioxidant</subject><subject>antioxidants</subject><subject>Antioxidants - pharmacology</subject><subject>apoptosis</subject><subject>Astilbin</subject><subject>caspase-3</subject><subject>catalase</subject><subject>epididymis</subject><subject>follicle-stimulating hormone</subject><subject>glutathione peroxidase</subject><subject>glutathione-disulfide reductase</subject><subject>histology</subject><subject>histopathology</subject><subject>interleukin-6</subject><subject>luteinizing hormone</subject><subject>Male</subject><subject>males</subject><subject>microplastics</subject><subject>Microplastics - metabolism</subject><subject>Microplastics - pharmacology</subject><subject>necrosis</subject><subject>neoplasms</subject><subject>Oxidative Stress</subject><subject>pharmacology</subject><subject>Plastics - metabolism</subject><subject>poisoning</subject><subject>Polystyrene Microplastics</subject><subject>polystyrenes</subject><subject>Polystyrenes - metabolism</subject><subject>Polystyrenes - toxicity</subject><subject>prostaglandin synthase</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Semen - metabolism</subject><subject>spermatozoa</subject><subject>superoxide dismutase</subject><subject>testes</subject><subject>Testicular Damage</subject><subject>Testis</subject><subject>testosterone</subject><subject>toxicity</subject><subject>viability</subject><issn>0041-008X</issn><issn>1096-0333</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc9u1DAQhy1ERbeFF-CAfORAlrGdv4hLqSggVeqllbhZjj3peuXEwXZW2kfhbXG0LUfEyfL4m5_G8xHylsGWAas_7rdJqXnLgYstY3VVdS_IhkFXFyCEeEk2ACUrANqf5-Qixj0AdGXJXpFz0XDWADQb8vsqJZwWlewBKQ4D6kT9QFVM1vV2on6is3fHmI4BJ6Sj1cHPbn3WkdrJLBoNTbjeF6cCNWpUj_iJfrFe7zDjyn2gccYwquSdf1wLVE2G7mxMflZp91wtehVzFh6swUljfE3OBuUivnk6L8nDzdf76-_F7d23H9dXt4UWbZcKFB0XNZRVa9q6g1KXnFel0YMxvWEtE3UtBqF6U3Uda3jZC2CD5qrVnJkBa3FJ3p9y5-B_LfkncrRRo3NqQr9EyVtR8qbi_H9Q1kKVBxAZ5Sc07yvGgIOcgx1VOEoGcrUn93K1J1d78mQvN717yl_6Ec3flmddGfh8AjAv5GAxyKjtui1jQzYnjbf_yv8DgemuzA</recordid><startdate>20230715</startdate><enddate>20230715</enddate><creator>Rizwan, Arooj</creator><creator>Ijaz, Muhammad Umar</creator><creator>Hamza, Ali</creator><creator>Anwar, Haseeb</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20230715</creationdate><title>Attenuative effect of astilbin on polystyrene microplastics induced testicular damage: Biochemical, spermatological and histopathological-based evidences</title><author>Rizwan, Arooj ; Ijaz, Muhammad Umar ; Hamza, Ali ; Anwar, Haseeb</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-e392360458d86904c42254dcfddbd1813663f3abd5991724b301fc2a8c21dfe63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>adults</topic><topic>Animals</topic><topic>Antioxidant</topic><topic>antioxidants</topic><topic>Antioxidants - pharmacology</topic><topic>apoptosis</topic><topic>Astilbin</topic><topic>caspase-3</topic><topic>catalase</topic><topic>epididymis</topic><topic>follicle-stimulating hormone</topic><topic>glutathione peroxidase</topic><topic>glutathione-disulfide reductase</topic><topic>histology</topic><topic>histopathology</topic><topic>interleukin-6</topic><topic>luteinizing hormone</topic><topic>Male</topic><topic>males</topic><topic>microplastics</topic><topic>Microplastics - metabolism</topic><topic>Microplastics - pharmacology</topic><topic>necrosis</topic><topic>neoplasms</topic><topic>Oxidative Stress</topic><topic>pharmacology</topic><topic>Plastics - metabolism</topic><topic>poisoning</topic><topic>Polystyrene Microplastics</topic><topic>polystyrenes</topic><topic>Polystyrenes - metabolism</topic><topic>Polystyrenes - toxicity</topic><topic>prostaglandin synthase</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Semen - metabolism</topic><topic>spermatozoa</topic><topic>superoxide dismutase</topic><topic>testes</topic><topic>Testicular Damage</topic><topic>Testis</topic><topic>testosterone</topic><topic>toxicity</topic><topic>viability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rizwan, Arooj</creatorcontrib><creatorcontrib>Ijaz, Muhammad Umar</creatorcontrib><creatorcontrib>Hamza, Ali</creatorcontrib><creatorcontrib>Anwar, Haseeb</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Toxicology and applied pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rizwan, Arooj</au><au>Ijaz, Muhammad Umar</au><au>Hamza, Ali</au><au>Anwar, Haseeb</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Attenuative effect of astilbin on polystyrene microplastics induced testicular damage: Biochemical, spermatological and histopathological-based evidences</atitle><jtitle>Toxicology and applied pharmacology</jtitle><addtitle>Toxicol Appl Pharmacol</addtitle><date>2023-07-15</date><risdate>2023</risdate><volume>471</volume><spage>116559</spage><epage>116559</epage><pages>116559-116559</pages><artnum>116559</artnum><issn>0041-008X</issn><eissn>1096-0333</eissn><abstract>Polystyrene microplastics (PS-MPs) are the potential environmental pollutants that possess the ability to induce testicular damage. Astilbin (ASB) is a dihydroflavonol, abundantly reported in multiple plants that has various pharmacological properties. This research elucidated the mitigative potential of ASB against PS-MPs-instigated testicular toxicity. 48 adult male rats (200 ± 10 g) were distributed into 4 groups (n = 12): control, PS-MPs received (0.01 mg/kg), PS-MPs + ASB received (0.01 mg/kg + 20 mg/kg) and ASB supplemented group (20 mg/kg). After 56th day of the trial, animals were sacrificed and testes were harvested for the estimation of biochemical, hormonal, spermatogenic, steroidogenic, apoptotic and histological profiles. PS-MPs intoxication significantly (P < 0.05) lowered glutathione peroxidase (GPx), superoxide dismutase (SOD), glutathione reductase (GSR) as well as catalase (CAT) activities, whereas elevated MDA as well as ROS levels. Besides, the levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), nuclear factor kappa-B (NF-κB) along with cyclooxygenase-2 (COX-2) activity were raised. PS-MPs treatment reduced luteinizing hormone (LH), plasma testosterone and follicle-stimulating hormone (FSH) level besides decreased epididymal sperm number, viability, motility as well as the count of HOS coil-tailed spermatozoa and increased sperm morphological irregularities. PS-MPs exposure lowered steroidogenic enzymes (17β-HSD, 3β-HSD and StAR protein along with Bcl-2 expression, besides increasing Caspase-3 and Bax expressions and histopathological alterations in testicular tissues. However, ASB treatment significantly reversed PS-MPs mediated damage. In conclusion, ASB administration is protective against PS-MPs-instigated testicular damage owing to its anti-inflammatory, anti-apoptotic, antioxidant and androgenic nature.
[Display omitted]
•PS-MPs exposure damaged biochemical, spermatogenic and histological profiles.•ASB increased antioxidant enzyme activities and decreased MDA and ROS levels.•ASB reversed the levels of inflammatory and apoptotic markers.•Moreover, ASB increased the expression of steroidogenic enzymes and hormone levels.•ASB also improved all the histopathological alterations in the testicular tissues.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>37217007</pmid><doi>10.1016/j.taap.2023.116559</doi><tpages>1</tpages></addata></record> |
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| subjects | adults Animals Antioxidant antioxidants Antioxidants - pharmacology apoptosis Astilbin caspase-3 catalase epididymis follicle-stimulating hormone glutathione peroxidase glutathione-disulfide reductase histology histopathology interleukin-6 luteinizing hormone Male males microplastics Microplastics - metabolism Microplastics - pharmacology necrosis neoplasms Oxidative Stress pharmacology Plastics - metabolism poisoning Polystyrene Microplastics polystyrenes Polystyrenes - metabolism Polystyrenes - toxicity prostaglandin synthase Rats Rats, Wistar Semen - metabolism spermatozoa superoxide dismutase testes Testicular Damage Testis testosterone toxicity viability |
| title | Attenuative effect of astilbin on polystyrene microplastics induced testicular damage: Biochemical, spermatological and histopathological-based evidences |
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