Development and validation of a TaqMan® probe- based real-time PCR assay for detection of Ehrlichia canis

Ehrlichiosis is a potentially fatal zoonotic tick-borne disease, caused by a pleomorphic Gram-negative bacterium. It occurs worldwide and affects humans, domestic and wild animals. Dogs infected with Ehrlichia canis develop canine monocytic ehrlichiosis (CME), a significant infectious disease of can...

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Veröffentlicht in:	Ticks and tick-borne diseases 2022-11, Vol.13 (6), p.102055, Article 102055
Hauptverfasser:	Nkosi, Nokuzola F., Oosthuizen, Marinda C., Quan, Melvyn
Format:	Artikel
Sprache:	eng
Schlagworte:	Bayesian theory blood Canine confidence interval detection limit diagnostic sensitivity dogs early diagnosis Ehrlichia canis Ehrlichiosis Gram-negative bacteria Haemoparasite hemoparasites Hydrolysis Minor groove binder (MGB) nucleic acid hybridization plasmids quantitative polymerase chain reaction standard deviation Tick-borne disease
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