RNA sequencing and metabolic analysis of imiquimod-induced psoriasis-like mice with chronic restrain stress
Psoriasis is one of the most common dermatological disorders, characterized by increased epidermal hyperplasia and immune cell infiltration. Psychological stress has been reported to contribute to the severity, aggravation, and relapse of psoriasis. However, the exact mechanism involved in psycholog...
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| Veröffentlicht in: | Life sciences (1973) 2023-08, Vol.326, p.121788-121788, Article 121788 |
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| container_title | Life sciences (1973) |
| container_volume | 326 |
| creator | Al Rudaisat, Mus'ab Chen, Xianzhen Chen, Siji Amanullah, Md Wang, Xuewen Liang, Qichang Hua, Chunting Zhou, Can Song, Yinjing van der Veen, Stijn Cheng, Hao |
| description | Psoriasis is one of the most common dermatological disorders, characterized by increased epidermal hyperplasia and immune cell infiltration. Psychological stress has been reported to contribute to the severity, aggravation, and relapse of psoriasis. However, the exact mechanism involved in psychological stress's impact on psoriasis is still unclear. We aim to investigate the role of psychological stress in psoriasis from a transcriptomic and metabolomic perspective.
We developed a chronic restrain stress (CRS)-imiquimod (IMQ)-induced psoriasis-like mouse model and performed a comprehensive comparative transcriptomic and metabolic analysis with control mice, CRS-treated mice, and IMQ-treated mice to investigate how psychological stress affects psoriasis.
We found that CRS-IMQ-induced psoriasis-like mice showed significant exacerbation of psoriasis-like skin inflammation compared with mice treated with IMQ only. Mice of the CRS + IMQ group showed increased expression of keratinocyte proliferation and differentiation genes, differential regulation of cytokines, and promotion of linoleic acid metabolism. Correlation analysis of differentially expressed genes in the CRS-IMQ-induced psoriasis-like mice and human psoriasis datasets compared with respective controls revealed 96 overlapping genes of which 30 genes showed consistent induced or repressed expression in all human and mouse datasets.
Our study provides new insights into the effects of psychological stress on psoriasis pathogenesis and the mechanisms involved, which provides clues for development of therapeutics or biomarkers. |
| doi_str_mv | 10.1016/j.lfs.2023.121788 |
| format | Article |
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We developed a chronic restrain stress (CRS)-imiquimod (IMQ)-induced psoriasis-like mouse model and performed a comprehensive comparative transcriptomic and metabolic analysis with control mice, CRS-treated mice, and IMQ-treated mice to investigate how psychological stress affects psoriasis.
We found that CRS-IMQ-induced psoriasis-like mice showed significant exacerbation of psoriasis-like skin inflammation compared with mice treated with IMQ only. Mice of the CRS + IMQ group showed increased expression of keratinocyte proliferation and differentiation genes, differential regulation of cytokines, and promotion of linoleic acid metabolism. Correlation analysis of differentially expressed genes in the CRS-IMQ-induced psoriasis-like mice and human psoriasis datasets compared with respective controls revealed 96 overlapping genes of which 30 genes showed consistent induced or repressed expression in all human and mouse datasets.
Our study provides new insights into the effects of psychological stress on psoriasis pathogenesis and the mechanisms involved, which provides clues for development of therapeutics or biomarkers.</description><identifier>ISSN: 0024-3205</identifier><identifier>EISSN: 1879-0631</identifier><identifier>DOI: 10.1016/j.lfs.2023.121788</identifier><identifier>PMID: 37230377</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Aminoquinolines - toxicity ; Animals ; Bioinformatics ; biomarkers ; Chronic psychological stress ; Chronic restrain stress ; cytokines ; data collection ; Disease Models, Animal ; gene expression regulation ; Humans ; hyperplasia ; Imiquimod - toxicity ; inflammation ; keratinocytes ; linoleic acid ; Metabolism ; metabolomics ; Mice ; Mice, Inbred BALB C ; pathogenesis ; Psoriasis ; Psoriasis - chemically induced ; Psoriasis - genetics ; psychological stress ; relapse ; RNA ; RNA-Seq ; Sequence Analysis, RNA ; Skin ; therapeutics ; transcriptomics</subject><ispartof>Life sciences (1973), 2023-08, Vol.326, p.121788-121788, Article 121788</ispartof><rights>2023 Elsevier Inc.</rights><rights>Copyright © 2023 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-f21b110fea52ae2fa76c7aadfb0fd9f41399f3bb7fc2ad7444f36006da22d8e33</citedby><cites>FETCH-LOGICAL-c386t-f21b110fea52ae2fa76c7aadfb0fd9f41399f3bb7fc2ad7444f36006da22d8e33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0024320523004228$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37230377$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Al Rudaisat, Mus'ab</creatorcontrib><creatorcontrib>Chen, Xianzhen</creatorcontrib><creatorcontrib>Chen, Siji</creatorcontrib><creatorcontrib>Amanullah, Md</creatorcontrib><creatorcontrib>Wang, Xuewen</creatorcontrib><creatorcontrib>Liang, Qichang</creatorcontrib><creatorcontrib>Hua, Chunting</creatorcontrib><creatorcontrib>Zhou, Can</creatorcontrib><creatorcontrib>Song, Yinjing</creatorcontrib><creatorcontrib>van der Veen, Stijn</creatorcontrib><creatorcontrib>Cheng, Hao</creatorcontrib><title>RNA sequencing and metabolic analysis of imiquimod-induced psoriasis-like mice with chronic restrain stress</title><title>Life sciences (1973)</title><addtitle>Life Sci</addtitle><description>Psoriasis is one of the most common dermatological disorders, characterized by increased epidermal hyperplasia and immune cell infiltration. Psychological stress has been reported to contribute to the severity, aggravation, and relapse of psoriasis. However, the exact mechanism involved in psychological stress's impact on psoriasis is still unclear. We aim to investigate the role of psychological stress in psoriasis from a transcriptomic and metabolomic perspective.
We developed a chronic restrain stress (CRS)-imiquimod (IMQ)-induced psoriasis-like mouse model and performed a comprehensive comparative transcriptomic and metabolic analysis with control mice, CRS-treated mice, and IMQ-treated mice to investigate how psychological stress affects psoriasis.
We found that CRS-IMQ-induced psoriasis-like mice showed significant exacerbation of psoriasis-like skin inflammation compared with mice treated with IMQ only. Mice of the CRS + IMQ group showed increased expression of keratinocyte proliferation and differentiation genes, differential regulation of cytokines, and promotion of linoleic acid metabolism. Correlation analysis of differentially expressed genes in the CRS-IMQ-induced psoriasis-like mice and human psoriasis datasets compared with respective controls revealed 96 overlapping genes of which 30 genes showed consistent induced or repressed expression in all human and mouse datasets.
Our study provides new insights into the effects of psychological stress on psoriasis pathogenesis and the mechanisms involved, which provides clues for development of therapeutics or biomarkers.</description><subject>Aminoquinolines - toxicity</subject><subject>Animals</subject><subject>Bioinformatics</subject><subject>biomarkers</subject><subject>Chronic psychological stress</subject><subject>Chronic restrain stress</subject><subject>cytokines</subject><subject>data collection</subject><subject>Disease Models, Animal</subject><subject>gene expression regulation</subject><subject>Humans</subject><subject>hyperplasia</subject><subject>Imiquimod - toxicity</subject><subject>inflammation</subject><subject>keratinocytes</subject><subject>linoleic acid</subject><subject>Metabolism</subject><subject>metabolomics</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>pathogenesis</subject><subject>Psoriasis</subject><subject>Psoriasis - chemically induced</subject><subject>Psoriasis - genetics</subject><subject>psychological stress</subject><subject>relapse</subject><subject>RNA</subject><subject>RNA-Seq</subject><subject>Sequence Analysis, RNA</subject><subject>Skin</subject><subject>therapeutics</subject><subject>transcriptomics</subject><issn>0024-3205</issn><issn>1879-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v1DAQxS1URJfCB-CCfOwly9hO4kScqqr8kSqQEJwtxx63s03irZ1Q9dvj1bYc4TQaze89ad5j7J2ArQDRfthtx5C3EqTaCil0171gG9HpvoJWiRO2AZB1pSQ0p-x1zjsAaBqtXrFTpaUCpfWG3f34dsEz3q84O5pvuJ09n3CxQxzJlc2Oj5kyj4HTRPcrTdFXNPvVoef7HBPZcq5GukM-kUP-QMstd7cpzkWeMC_J0szLwJzfsJfBjhnfPs0z9uvT1c_LL9X1989fLy-uK6e6dqmCFIMQENA20qIMVrdOW-vDAMH3oRaq74MaBh2ctF7XdR1UC9B6K6XvUKkzdn703adYHsuLmSg7HEc7Y1yzkZ2qZdOLktL_UQmgQEtRUHFEXYo5Jwxmn2iy6dEIMIc6zM6UOsyhDnOso2jeP9mvw4T-r-I5_wJ8PAJY8vhNmEx2VKpATwndYnykf9j_AY23nJc</recordid><startdate>20230801</startdate><enddate>20230801</enddate><creator>Al Rudaisat, Mus'ab</creator><creator>Chen, Xianzhen</creator><creator>Chen, Siji</creator><creator>Amanullah, Md</creator><creator>Wang, Xuewen</creator><creator>Liang, Qichang</creator><creator>Hua, Chunting</creator><creator>Zhou, Can</creator><creator>Song, Yinjing</creator><creator>van der Veen, Stijn</creator><creator>Cheng, Hao</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20230801</creationdate><title>RNA sequencing and metabolic analysis of imiquimod-induced psoriasis-like mice with chronic restrain stress</title><author>Al Rudaisat, Mus'ab ; Chen, Xianzhen ; Chen, Siji ; Amanullah, Md ; Wang, Xuewen ; Liang, Qichang ; Hua, Chunting ; Zhou, Can ; Song, Yinjing ; van der Veen, Stijn ; Cheng, Hao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-f21b110fea52ae2fa76c7aadfb0fd9f41399f3bb7fc2ad7444f36006da22d8e33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Aminoquinolines - toxicity</topic><topic>Animals</topic><topic>Bioinformatics</topic><topic>biomarkers</topic><topic>Chronic psychological stress</topic><topic>Chronic restrain stress</topic><topic>cytokines</topic><topic>data collection</topic><topic>Disease Models, Animal</topic><topic>gene expression regulation</topic><topic>Humans</topic><topic>hyperplasia</topic><topic>Imiquimod - toxicity</topic><topic>inflammation</topic><topic>keratinocytes</topic><topic>linoleic acid</topic><topic>Metabolism</topic><topic>metabolomics</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>pathogenesis</topic><topic>Psoriasis</topic><topic>Psoriasis - chemically induced</topic><topic>Psoriasis - genetics</topic><topic>psychological stress</topic><topic>relapse</topic><topic>RNA</topic><topic>RNA-Seq</topic><topic>Sequence Analysis, RNA</topic><topic>Skin</topic><topic>therapeutics</topic><topic>transcriptomics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Al Rudaisat, Mus'ab</creatorcontrib><creatorcontrib>Chen, Xianzhen</creatorcontrib><creatorcontrib>Chen, Siji</creatorcontrib><creatorcontrib>Amanullah, Md</creatorcontrib><creatorcontrib>Wang, Xuewen</creatorcontrib><creatorcontrib>Liang, Qichang</creatorcontrib><creatorcontrib>Hua, Chunting</creatorcontrib><creatorcontrib>Zhou, Can</creatorcontrib><creatorcontrib>Song, Yinjing</creatorcontrib><creatorcontrib>van der Veen, Stijn</creatorcontrib><creatorcontrib>Cheng, Hao</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Life sciences (1973)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Al Rudaisat, Mus'ab</au><au>Chen, Xianzhen</au><au>Chen, Siji</au><au>Amanullah, Md</au><au>Wang, Xuewen</au><au>Liang, Qichang</au><au>Hua, Chunting</au><au>Zhou, Can</au><au>Song, Yinjing</au><au>van der Veen, Stijn</au><au>Cheng, Hao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>RNA sequencing and metabolic analysis of imiquimod-induced psoriasis-like mice with chronic restrain stress</atitle><jtitle>Life sciences (1973)</jtitle><addtitle>Life Sci</addtitle><date>2023-08-01</date><risdate>2023</risdate><volume>326</volume><spage>121788</spage><epage>121788</epage><pages>121788-121788</pages><artnum>121788</artnum><issn>0024-3205</issn><eissn>1879-0631</eissn><abstract>Psoriasis is one of the most common dermatological disorders, characterized by increased epidermal hyperplasia and immune cell infiltration. Psychological stress has been reported to contribute to the severity, aggravation, and relapse of psoriasis. However, the exact mechanism involved in psychological stress's impact on psoriasis is still unclear. We aim to investigate the role of psychological stress in psoriasis from a transcriptomic and metabolomic perspective.
We developed a chronic restrain stress (CRS)-imiquimod (IMQ)-induced psoriasis-like mouse model and performed a comprehensive comparative transcriptomic and metabolic analysis with control mice, CRS-treated mice, and IMQ-treated mice to investigate how psychological stress affects psoriasis.
We found that CRS-IMQ-induced psoriasis-like mice showed significant exacerbation of psoriasis-like skin inflammation compared with mice treated with IMQ only. Mice of the CRS + IMQ group showed increased expression of keratinocyte proliferation and differentiation genes, differential regulation of cytokines, and promotion of linoleic acid metabolism. Correlation analysis of differentially expressed genes in the CRS-IMQ-induced psoriasis-like mice and human psoriasis datasets compared with respective controls revealed 96 overlapping genes of which 30 genes showed consistent induced or repressed expression in all human and mouse datasets.
Our study provides new insights into the effects of psychological stress on psoriasis pathogenesis and the mechanisms involved, which provides clues for development of therapeutics or biomarkers.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>37230377</pmid><doi>10.1016/j.lfs.2023.121788</doi><tpages>1</tpages></addata></record> |
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| ispartof | Life sciences (1973), 2023-08, Vol.326, p.121788-121788, Article 121788 |
| issn | 0024-3205 1879-0631 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Aminoquinolines - toxicity Animals Bioinformatics biomarkers Chronic psychological stress Chronic restrain stress cytokines data collection Disease Models, Animal gene expression regulation Humans hyperplasia Imiquimod - toxicity inflammation keratinocytes linoleic acid Metabolism metabolomics Mice Mice, Inbred BALB C pathogenesis Psoriasis Psoriasis - chemically induced Psoriasis - genetics psychological stress relapse RNA RNA-Seq Sequence Analysis, RNA Skin therapeutics transcriptomics |
| title | RNA sequencing and metabolic analysis of imiquimod-induced psoriasis-like mice with chronic restrain stress |
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