Genome-wide identification and characterization of Toll-like receptors (TLR) genes in Haliotis discus hannai, H. rufescens, and H. laevigata
Toll-like receptors (TLRs) play essential roles in the innate immune system and have been extensively studied in mollusks. In this study, through a genome-wide search, TLR genes were identified as 29 in Haliotis discus hannai, 33 in H. rufescens, and 16 in H. laevigata. Domain analysis indicated tha...
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| Veröffentlicht in: | Fish & shellfish immunology 2023-06, Vol.137, p.108728-108728, Article 108728 |
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| description | Toll-like receptors (TLRs) play essential roles in the innate immune system and have been extensively studied in mollusks. In this study, through a genome-wide search, TLR genes were identified as 29 in Haliotis discus hannai, 33 in H. rufescens, and 16 in H. laevigata. Domain analysis indicated that these TLR genes contain leucine-rich repeat (LRR) and Toll/IL-1 receptor (TIR) domains and exons ranging from 1 to 5. Polymorphism analysis showed that the TLRs in abalones did not have high diversities with 143 SNPs and no Indel in H. discus hannai, 92 SNPs and 3 Indels together with 6 missense mutations in H. rufescens, and no SNP or Indel in H. laevigata. The expression of 8 TLR genes in H. discus hannai was confirmed in the hepatopancreas, gill, hemolymph, gonads, intestine, muscle, and mantle. The expression of five TLR genes (out of 8) in gills (p |
| doi_str_mv | 10.1016/j.fsi.2023.108728 |
| format | Article |
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•29, 33, 16 TLRs were identified from the genomes of Haliotis discus hannai H. rufescens and H. laevigata, separately.•Abalone TLR genes contain leucine-rich repeat (LRR) and Toll/IL-1 receptor (TIR) domains and exons ranging from 1 to 5.•143 SNPs and no Indel in H. discus hannai, 92 SNPs and 3 Indels together with 6 missense mutations in H. rufescens, and no SNP or Indel in H. laevigata.•Vibrio exposure regulates the expression of TLRs in the gills, hepatopancreas, and hemolymph of H. discus hannai.</description><identifier>ISSN: 1050-4648</identifier><identifier>EISSN: 1095-9947</identifier><identifier>DOI: 10.1016/j.fsi.2023.108728</identifier><identifier>PMID: 37011737</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; domain ; Exons ; fish ; Gastropoda - genetics ; Gene expression ; Genome ; H. laevigata ; H. rufescens ; Haliotis discus hannai ; hemolymph ; hepatopancreas ; innate immunity ; intestines ; muscles ; shellfish ; Toll-Like Receptors ; Toll-like receptors (TLRs) ; Vibrio parahaemolyticus ; Vibrio parahaemolyticus - physiology</subject><ispartof>Fish & shellfish immunology, 2023-06, Vol.137, p.108728-108728, Article 108728</ispartof><rights>2023 Elsevier Ltd</rights><rights>Copyright © 2023 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-70791922586d11486e0cce9df8c63d610dde7f434e892c374a60a16e9ea13b8a3</citedby><cites>FETCH-LOGICAL-c386t-70791922586d11486e0cce9df8c63d610dde7f434e892c374a60a16e9ea13b8a3</cites><orcidid>0000-0003-2802-1586</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1050464823002140$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37011737$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zou, Yuelian</creatorcontrib><creatorcontrib>Xu, Xin</creatorcontrib><creatorcontrib>Xiao, Xiaotian</creatorcontrib><creatorcontrib>Wang, Yilei</creatorcontrib><creatorcontrib>Yang, Huiping</creatorcontrib><creatorcontrib>Zhang, Ziping</creatorcontrib><title>Genome-wide identification and characterization of Toll-like receptors (TLR) genes in Haliotis discus hannai, H. rufescens, and H. laevigata</title><title>Fish & shellfish immunology</title><addtitle>Fish Shellfish Immunol</addtitle><description>Toll-like receptors (TLRs) play essential roles in the innate immune system and have been extensively studied in mollusks. In this study, through a genome-wide search, TLR genes were identified as 29 in Haliotis discus hannai, 33 in H. rufescens, and 16 in H. laevigata. Domain analysis indicated that these TLR genes contain leucine-rich repeat (LRR) and Toll/IL-1 receptor (TIR) domains and exons ranging from 1 to 5. Polymorphism analysis showed that the TLRs in abalones did not have high diversities with 143 SNPs and no Indel in H. discus hannai, 92 SNPs and 3 Indels together with 6 missense mutations in H. rufescens, and no SNP or Indel in H. laevigata. The expression of 8 TLR genes in H. discus hannai was confirmed in the hepatopancreas, gill, hemolymph, gonads, intestine, muscle, and mantle. The expression of five TLR genes (out of 8) in gills (p < 0.05), three in hepatopancreas (p < 0.05), and three in hemolymph (p < 0.05) was upregulated separately in response to the infection caused by Vibrio parahaemolyticus. The findings in this study would contribute to a better understanding of the molecular immune mechanism of H. discus hannai against stimulation by V. parahaemolyticus and provide a basis for the study of TLRs in abalones.
•29, 33, 16 TLRs were identified from the genomes of Haliotis discus hannai H. rufescens and H. laevigata, separately.•Abalone TLR genes contain leucine-rich repeat (LRR) and Toll/IL-1 receptor (TIR) domains and exons ranging from 1 to 5.•143 SNPs and no Indel in H. discus hannai, 92 SNPs and 3 Indels together with 6 missense mutations in H. rufescens, and no SNP or Indel in H. laevigata.•Vibrio exposure regulates the expression of TLRs in the gills, hepatopancreas, and hemolymph of H. discus hannai.</description><subject>Animals</subject><subject>domain</subject><subject>Exons</subject><subject>fish</subject><subject>Gastropoda - genetics</subject><subject>Gene expression</subject><subject>Genome</subject><subject>H. laevigata</subject><subject>H. rufescens</subject><subject>Haliotis discus hannai</subject><subject>hemolymph</subject><subject>hepatopancreas</subject><subject>innate immunity</subject><subject>intestines</subject><subject>muscles</subject><subject>shellfish</subject><subject>Toll-Like Receptors</subject><subject>Toll-like receptors (TLRs)</subject><subject>Vibrio parahaemolyticus</subject><subject>Vibrio parahaemolyticus - physiology</subject><issn>1050-4648</issn><issn>1095-9947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV1rFDEUhgdRbK3-AG8klxU6a74mH3hVinaFBUHW65AmZ9qss8maZCr6G_zRZp3qpV6Ekxze8-bwPl33kuAVwUS82a3GElYUU9beSlL1qDslWA-91lw-Pt4H3HPB1Un3rJQdxlgwgZ92J0xiQiSTp93Pa4hpD_234AG1E2sYg7M1pIhs9Mjd2WxdhRx-LM00om2apn4KXwBlcHCoKRd0vt18eo1uIUJBIaK1nUKqoSAfipsLurMx2nCB1iuU5xGKg1gufn_QOpOF-3Brq33ePRntVODFQz3rPr9_t71a95uP1x-uLje9Y0rUXmKpiaZ0UMITwpUA7BxoPyonmBcEew9y5IyD0tQxya3AlgjQYAm7UZaddeeL7yGnrzOUavZtTZgmGyHNxVDFOOVKk-H_UqmHlimmuEnJInU5lZJhNIcc9jZ_NwSbIy-zM42XOfIyC6828-rBfr7Zg_878QdQE7xdBNDyuA-QTXEBogMfWvjV-BT-Yf8LAWilTg</recordid><startdate>202306</startdate><enddate>202306</enddate><creator>Zou, Yuelian</creator><creator>Xu, Xin</creator><creator>Xiao, Xiaotian</creator><creator>Wang, Yilei</creator><creator>Yang, Huiping</creator><creator>Zhang, Ziping</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0003-2802-1586</orcidid></search><sort><creationdate>202306</creationdate><title>Genome-wide identification and characterization of Toll-like receptors (TLR) genes in Haliotis discus hannai, H. rufescens, and H. laevigata</title><author>Zou, Yuelian ; Xu, Xin ; Xiao, Xiaotian ; Wang, Yilei ; Yang, Huiping ; Zhang, Ziping</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-70791922586d11486e0cce9df8c63d610dde7f434e892c374a60a16e9ea13b8a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animals</topic><topic>domain</topic><topic>Exons</topic><topic>fish</topic><topic>Gastropoda - genetics</topic><topic>Gene expression</topic><topic>Genome</topic><topic>H. laevigata</topic><topic>H. rufescens</topic><topic>Haliotis discus hannai</topic><topic>hemolymph</topic><topic>hepatopancreas</topic><topic>innate immunity</topic><topic>intestines</topic><topic>muscles</topic><topic>shellfish</topic><topic>Toll-Like Receptors</topic><topic>Toll-like receptors (TLRs)</topic><topic>Vibrio parahaemolyticus</topic><topic>Vibrio parahaemolyticus - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zou, Yuelian</creatorcontrib><creatorcontrib>Xu, Xin</creatorcontrib><creatorcontrib>Xiao, Xiaotian</creatorcontrib><creatorcontrib>Wang, Yilei</creatorcontrib><creatorcontrib>Yang, Huiping</creatorcontrib><creatorcontrib>Zhang, Ziping</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Fish & shellfish immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zou, Yuelian</au><au>Xu, Xin</au><au>Xiao, Xiaotian</au><au>Wang, Yilei</au><au>Yang, Huiping</au><au>Zhang, Ziping</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genome-wide identification and characterization of Toll-like receptors (TLR) genes in Haliotis discus hannai, H. rufescens, and H. laevigata</atitle><jtitle>Fish & shellfish immunology</jtitle><addtitle>Fish Shellfish Immunol</addtitle><date>2023-06</date><risdate>2023</risdate><volume>137</volume><spage>108728</spage><epage>108728</epage><pages>108728-108728</pages><artnum>108728</artnum><issn>1050-4648</issn><eissn>1095-9947</eissn><abstract>Toll-like receptors (TLRs) play essential roles in the innate immune system and have been extensively studied in mollusks. In this study, through a genome-wide search, TLR genes were identified as 29 in Haliotis discus hannai, 33 in H. rufescens, and 16 in H. laevigata. Domain analysis indicated that these TLR genes contain leucine-rich repeat (LRR) and Toll/IL-1 receptor (TIR) domains and exons ranging from 1 to 5. Polymorphism analysis showed that the TLRs in abalones did not have high diversities with 143 SNPs and no Indel in H. discus hannai, 92 SNPs and 3 Indels together with 6 missense mutations in H. rufescens, and no SNP or Indel in H. laevigata. The expression of 8 TLR genes in H. discus hannai was confirmed in the hepatopancreas, gill, hemolymph, gonads, intestine, muscle, and mantle. The expression of five TLR genes (out of 8) in gills (p < 0.05), three in hepatopancreas (p < 0.05), and three in hemolymph (p < 0.05) was upregulated separately in response to the infection caused by Vibrio parahaemolyticus. The findings in this study would contribute to a better understanding of the molecular immune mechanism of H. discus hannai against stimulation by V. parahaemolyticus and provide a basis for the study of TLRs in abalones.
•29, 33, 16 TLRs were identified from the genomes of Haliotis discus hannai H. rufescens and H. laevigata, separately.•Abalone TLR genes contain leucine-rich repeat (LRR) and Toll/IL-1 receptor (TIR) domains and exons ranging from 1 to 5.•143 SNPs and no Indel in H. discus hannai, 92 SNPs and 3 Indels together with 6 missense mutations in H. rufescens, and no SNP or Indel in H. laevigata.•Vibrio exposure regulates the expression of TLRs in the gills, hepatopancreas, and hemolymph of H. discus hannai.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>37011737</pmid><doi>10.1016/j.fsi.2023.108728</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0003-2802-1586</orcidid></addata></record> |
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| ispartof | Fish & shellfish immunology, 2023-06, Vol.137, p.108728-108728, Article 108728 |
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| subjects | Animals domain Exons fish Gastropoda - genetics Gene expression Genome H. laevigata H. rufescens Haliotis discus hannai hemolymph hepatopancreas innate immunity intestines muscles shellfish Toll-Like Receptors Toll-like receptors (TLRs) Vibrio parahaemolyticus Vibrio parahaemolyticus - physiology |
| title | Genome-wide identification and characterization of Toll-like receptors (TLR) genes in Haliotis discus hannai, H. rufescens, and H. laevigata |
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