Propagation and immunological characterization of coxsackievirus A10 in a serum-free HEK293A cell culture system

•Many coxsackievirus A10 isolates have low culture efficiency in vero cells.•HEK293A cell is a suitable cell line for coxsackievirus A10 propagation.•A serum-free HEK293A cell culture was developed for coxsackievirus A10 production.•Other enteroviruses could also be propagated in serum-free HEK293A...

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Veröffentlicht in:	Virus research 2023-05, Vol.329, p.199101-199101, Article 199101
Hauptverfasser:	Lien, Sheng-Chieh, Shen, Yu-Sheng, Lin, Hsiao-Yu, Wu, Shang-Rung, Fang, Chih-Yeu, Chen, Chi-Hsun, Chen, Yi-An, Chong, Pele Choi-Sing, Huang, Ming-Hsi, Chow, Yen-Hung, Wang, Jen-Ren, Wu, Suh-Chin, Liu, Chia-Chyi
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals antibodies Antibodies, Neutralizing Antibodies, Viral cell culture Coxsackievirus A10 (CVA10) Enterovirus Enterovirus A Enterovirus A, Human - genetics Hand foot and mouth diseases Hand, Foot and Mouth Disease - prevention & control HEK293A immunogenicity Inactivated whole virion vaccine manufacturing Mice mouth Serum-free cell culture ultracentrifugation Vaccines, Inactivated viral vaccines viruses
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