Cinnamomum verum extract inhibits NOX2/ROS and PKCδ/JNK/AP-1/NF-κB pathway-mediated inflammatory response in PMA-stimulated THP-1 monocytes
Cinnamomum verum J. Presl (Cinnamon) is widely used in the food and pharmaceutical industries. C. verum exhibits various biological activities. However, it is unclear whether C. verum can inhibit NOX, a major source of ROS generation, and exert anti-inflammatory and antioxidant effects in PMA-stimul...
Gespeichert in:
| Veröffentlicht in: | Phytomedicine (Stuttgart) 2023-04, Vol.112, p.154685-154685, Article 154685 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 154685 |
|---|---|
| container_issue | |
| container_start_page | 154685 |
| container_title | Phytomedicine (Stuttgart) |
| container_volume | 112 |
| creator | Kim, Na-Yeon Kim, Seonhwa Park, Hyo-Min Lim, Chae-Min Kim, Jinju Park, Jae-Young Jeon, Kyeong-Bae Poudel, Amrit Lee, Hee Pom Oh, Sei-Ryang Ahn, Jongmin Yoon, Do-Young |
| description | Cinnamomum verum J. Presl (Cinnamon) is widely used in the food and pharmaceutical industries. C. verum exhibits various biological activities. However, it is unclear whether C. verum can inhibit NOX, a major source of ROS generation, and exert anti-inflammatory and antioxidant effects in PMA-stimulated THP-1 cells.
This study investigates the anti-inflammatory and antioxidant effects of C. verum in PMA-stimulated THP-1 cells.
The MeOH extract of C. verum was analyzed using UPLC-QTOF/MS. Anti-inflammatory and antioxidant effects of C. verum extract were examined by DCF-DA staining, immunofluorescence staining, RT-PCR, and immunoblotting in PMA-stimulated THP-1 cells.
C. verum and its components, cinnamic acid and coumarin, significantly attenuated the expression of IL-1β, IL-8, CCL5, and COX-2 in PMA-stimulated THP-1. C. verum decreased ROS levels via NOX2 downregulation, as well as ameliorated plasma membrane translocation of PKCδ and decreased JNK phosphorylation. Besides, C. verum suppressed the nuclear translocation of AP-1 and NF-κB, which modulates diverse pro-inflammatory genes.
C. verum effectively inhibits inflammation and oxidative stress during monocyte-macrophage differentiation and downregulates inflammatory mediators via NOX2/ROS and PKCδ/JNK/AP-1/NF-κB signaling.
[Display omitted] |
| doi_str_mv | 10.1016/j.phymed.2023.154685 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2834220087</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0944711323000454</els_id><sourcerecordid>2774897565</sourcerecordid><originalsourceid>FETCH-LOGICAL-c441t-d00c24b46e89ec2b5a308f4f34fe3ea536e0bb7993d2caaee3f50fbed2030a283</originalsourceid><addsrcrecordid>eNqFkctu1DAUhi1ERYfCGyCUJZtMfMttgzSMKIWWmREUqTvLcU40HsVJsJ1CHoK3YcVD9JnwNO0WNrZkf_85R-dD6BXBS4JJlhyWw34yUC8ppmxJUp4V6RO0IBkpYlymN0_RApecxzkh7BQ9d-6AMeFljp-hU5blKStovkC_1rrrpOnNaKJbsOGEn95K5SPd7XWlvYs22xuafNl-jWRXR7vL9d3v5NPmMlntYpJszuO7P--iQfr9DznFYRwtPdQh3LTSGOl7O0UW3NB3DsJrtPu8ip3XZmzvueuLUCUyfderyYN7gU4a2Tp4-XCfoW_n76_XF_HV9sPH9eoqVpwTH9cYK8ornkFRgqJVKhkuGt4w3gADmbIMcFXlZclqqqQEYE2KmwpqihmWtGBn6M1cd7D99xGcF0Y7BW0rO-hHJwLCKcW4yP-P5jkvyjzN0oDyGVW2d85CIwarjbSTIFgcnYmDmJ2JozMxOwux1w8dxur49xh6lBSAtzMAYSW3GqxwSkOnwrItKC_qXv-7w1--l6tn</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2774897565</pqid></control><display><type>article</type><title>Cinnamomum verum extract inhibits NOX2/ROS and PKCδ/JNK/AP-1/NF-κB pathway-mediated inflammatory response in PMA-stimulated THP-1 monocytes</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Kim, Na-Yeon ; Kim, Seonhwa ; Park, Hyo-Min ; Lim, Chae-Min ; Kim, Jinju ; Park, Jae-Young ; Jeon, Kyeong-Bae ; Poudel, Amrit ; Lee, Hee Pom ; Oh, Sei-Ryang ; Ahn, Jongmin ; Yoon, Do-Young</creator><creatorcontrib>Kim, Na-Yeon ; Kim, Seonhwa ; Park, Hyo-Min ; Lim, Chae-Min ; Kim, Jinju ; Park, Jae-Young ; Jeon, Kyeong-Bae ; Poudel, Amrit ; Lee, Hee Pom ; Oh, Sei-Ryang ; Ahn, Jongmin ; Yoon, Do-Young</creatorcontrib><description>Cinnamomum verum J. Presl (Cinnamon) is widely used in the food and pharmaceutical industries. C. verum exhibits various biological activities. However, it is unclear whether C. verum can inhibit NOX, a major source of ROS generation, and exert anti-inflammatory and antioxidant effects in PMA-stimulated THP-1 cells.
This study investigates the anti-inflammatory and antioxidant effects of C. verum in PMA-stimulated THP-1 cells.
The MeOH extract of C. verum was analyzed using UPLC-QTOF/MS. Anti-inflammatory and antioxidant effects of C. verum extract were examined by DCF-DA staining, immunofluorescence staining, RT-PCR, and immunoblotting in PMA-stimulated THP-1 cells.
C. verum and its components, cinnamic acid and coumarin, significantly attenuated the expression of IL-1β, IL-8, CCL5, and COX-2 in PMA-stimulated THP-1. C. verum decreased ROS levels via NOX2 downregulation, as well as ameliorated plasma membrane translocation of PKCδ and decreased JNK phosphorylation. Besides, C. verum suppressed the nuclear translocation of AP-1 and NF-κB, which modulates diverse pro-inflammatory genes.
C. verum effectively inhibits inflammation and oxidative stress during monocyte-macrophage differentiation and downregulates inflammatory mediators via NOX2/ROS and PKCδ/JNK/AP-1/NF-κB signaling.
[Display omitted]</description><identifier>ISSN: 0944-7113</identifier><identifier>EISSN: 1618-095X</identifier><identifier>DOI: 10.1016/j.phymed.2023.154685</identifier><identifier>PMID: 36753827</identifier><language>eng</language><publisher>Germany: Elsevier GmbH</publisher><subject>Anti-inflammation ; Anti-Inflammatory Agents - metabolism ; Anti-Inflammatory Agents - pharmacology ; Antioxidant ; antioxidants ; Antioxidants - pharmacology ; cinnamic acid ; Cinnamic acid, Coumarin ; Cinnamomum verum ; Cinnamomum zeylanicum ; Cinnamon ; coumarin ; fluorescent antibody technique ; immunoblotting ; inflammation ; interleukin-8 ; Lipopolysaccharides - pharmacology ; Monocytes ; NF-kappa B - metabolism ; oxidative stress ; phosphorylation ; plasma membrane ; Reactive Oxygen Species - metabolism ; Signal Transduction ; Transcription Factor AP-1 - metabolism</subject><ispartof>Phytomedicine (Stuttgart), 2023-04, Vol.112, p.154685-154685, Article 154685</ispartof><rights>2023 The Author(s)</rights><rights>Copyright © 2023 The Author(s). Published by Elsevier GmbH.. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c441t-d00c24b46e89ec2b5a308f4f34fe3ea536e0bb7993d2caaee3f50fbed2030a283</citedby><cites>FETCH-LOGICAL-c441t-d00c24b46e89ec2b5a308f4f34fe3ea536e0bb7993d2caaee3f50fbed2030a283</cites><orcidid>0000-0002-9362-7384 ; 0000-0003-4979-3702 ; 0000-0002-0154-614X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0944711323000454$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36753827$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Na-Yeon</creatorcontrib><creatorcontrib>Kim, Seonhwa</creatorcontrib><creatorcontrib>Park, Hyo-Min</creatorcontrib><creatorcontrib>Lim, Chae-Min</creatorcontrib><creatorcontrib>Kim, Jinju</creatorcontrib><creatorcontrib>Park, Jae-Young</creatorcontrib><creatorcontrib>Jeon, Kyeong-Bae</creatorcontrib><creatorcontrib>Poudel, Amrit</creatorcontrib><creatorcontrib>Lee, Hee Pom</creatorcontrib><creatorcontrib>Oh, Sei-Ryang</creatorcontrib><creatorcontrib>Ahn, Jongmin</creatorcontrib><creatorcontrib>Yoon, Do-Young</creatorcontrib><title>Cinnamomum verum extract inhibits NOX2/ROS and PKCδ/JNK/AP-1/NF-κB pathway-mediated inflammatory response in PMA-stimulated THP-1 monocytes</title><title>Phytomedicine (Stuttgart)</title><addtitle>Phytomedicine</addtitle><description>Cinnamomum verum J. Presl (Cinnamon) is widely used in the food and pharmaceutical industries. C. verum exhibits various biological activities. However, it is unclear whether C. verum can inhibit NOX, a major source of ROS generation, and exert anti-inflammatory and antioxidant effects in PMA-stimulated THP-1 cells.
This study investigates the anti-inflammatory and antioxidant effects of C. verum in PMA-stimulated THP-1 cells.
The MeOH extract of C. verum was analyzed using UPLC-QTOF/MS. Anti-inflammatory and antioxidant effects of C. verum extract were examined by DCF-DA staining, immunofluorescence staining, RT-PCR, and immunoblotting in PMA-stimulated THP-1 cells.
C. verum and its components, cinnamic acid and coumarin, significantly attenuated the expression of IL-1β, IL-8, CCL5, and COX-2 in PMA-stimulated THP-1. C. verum decreased ROS levels via NOX2 downregulation, as well as ameliorated plasma membrane translocation of PKCδ and decreased JNK phosphorylation. Besides, C. verum suppressed the nuclear translocation of AP-1 and NF-κB, which modulates diverse pro-inflammatory genes.
C. verum effectively inhibits inflammation and oxidative stress during monocyte-macrophage differentiation and downregulates inflammatory mediators via NOX2/ROS and PKCδ/JNK/AP-1/NF-κB signaling.
[Display omitted]</description><subject>Anti-inflammation</subject><subject>Anti-Inflammatory Agents - metabolism</subject><subject>Anti-Inflammatory Agents - pharmacology</subject><subject>Antioxidant</subject><subject>antioxidants</subject><subject>Antioxidants - pharmacology</subject><subject>cinnamic acid</subject><subject>Cinnamic acid, Coumarin</subject><subject>Cinnamomum verum</subject><subject>Cinnamomum zeylanicum</subject><subject>Cinnamon</subject><subject>coumarin</subject><subject>fluorescent antibody technique</subject><subject>immunoblotting</subject><subject>inflammation</subject><subject>interleukin-8</subject><subject>Lipopolysaccharides - pharmacology</subject><subject>Monocytes</subject><subject>NF-kappa B - metabolism</subject><subject>oxidative stress</subject><subject>phosphorylation</subject><subject>plasma membrane</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Signal Transduction</subject><subject>Transcription Factor AP-1 - metabolism</subject><issn>0944-7113</issn><issn>1618-095X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctu1DAUhi1ERYfCGyCUJZtMfMttgzSMKIWWmREUqTvLcU40HsVJsJ1CHoK3YcVD9JnwNO0WNrZkf_85R-dD6BXBS4JJlhyWw34yUC8ppmxJUp4V6RO0IBkpYlymN0_RApecxzkh7BQ9d-6AMeFljp-hU5blKStovkC_1rrrpOnNaKJbsOGEn95K5SPd7XWlvYs22xuafNl-jWRXR7vL9d3v5NPmMlntYpJszuO7P--iQfr9DznFYRwtPdQh3LTSGOl7O0UW3NB3DsJrtPu8ip3XZmzvueuLUCUyfderyYN7gU4a2Tp4-XCfoW_n76_XF_HV9sPH9eoqVpwTH9cYK8ornkFRgqJVKhkuGt4w3gADmbIMcFXlZclqqqQEYE2KmwpqihmWtGBn6M1cd7D99xGcF0Y7BW0rO-hHJwLCKcW4yP-P5jkvyjzN0oDyGVW2d85CIwarjbSTIFgcnYmDmJ2JozMxOwux1w8dxur49xh6lBSAtzMAYSW3GqxwSkOnwrItKC_qXv-7w1--l6tn</recordid><startdate>202304</startdate><enddate>202304</enddate><creator>Kim, Na-Yeon</creator><creator>Kim, Seonhwa</creator><creator>Park, Hyo-Min</creator><creator>Lim, Chae-Min</creator><creator>Kim, Jinju</creator><creator>Park, Jae-Young</creator><creator>Jeon, Kyeong-Bae</creator><creator>Poudel, Amrit</creator><creator>Lee, Hee Pom</creator><creator>Oh, Sei-Ryang</creator><creator>Ahn, Jongmin</creator><creator>Yoon, Do-Young</creator><general>Elsevier GmbH</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0002-9362-7384</orcidid><orcidid>https://orcid.org/0000-0003-4979-3702</orcidid><orcidid>https://orcid.org/0000-0002-0154-614X</orcidid></search><sort><creationdate>202304</creationdate><title>Cinnamomum verum extract inhibits NOX2/ROS and PKCδ/JNK/AP-1/NF-κB pathway-mediated inflammatory response in PMA-stimulated THP-1 monocytes</title><author>Kim, Na-Yeon ; Kim, Seonhwa ; Park, Hyo-Min ; Lim, Chae-Min ; Kim, Jinju ; Park, Jae-Young ; Jeon, Kyeong-Bae ; Poudel, Amrit ; Lee, Hee Pom ; Oh, Sei-Ryang ; Ahn, Jongmin ; Yoon, Do-Young</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c441t-d00c24b46e89ec2b5a308f4f34fe3ea536e0bb7993d2caaee3f50fbed2030a283</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Anti-inflammation</topic><topic>Anti-Inflammatory Agents - metabolism</topic><topic>Anti-Inflammatory Agents - pharmacology</topic><topic>Antioxidant</topic><topic>antioxidants</topic><topic>Antioxidants - pharmacology</topic><topic>cinnamic acid</topic><topic>Cinnamic acid, Coumarin</topic><topic>Cinnamomum verum</topic><topic>Cinnamomum zeylanicum</topic><topic>Cinnamon</topic><topic>coumarin</topic><topic>fluorescent antibody technique</topic><topic>immunoblotting</topic><topic>inflammation</topic><topic>interleukin-8</topic><topic>Lipopolysaccharides - pharmacology</topic><topic>Monocytes</topic><topic>NF-kappa B - metabolism</topic><topic>oxidative stress</topic><topic>phosphorylation</topic><topic>plasma membrane</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Signal Transduction</topic><topic>Transcription Factor AP-1 - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Na-Yeon</creatorcontrib><creatorcontrib>Kim, Seonhwa</creatorcontrib><creatorcontrib>Park, Hyo-Min</creatorcontrib><creatorcontrib>Lim, Chae-Min</creatorcontrib><creatorcontrib>Kim, Jinju</creatorcontrib><creatorcontrib>Park, Jae-Young</creatorcontrib><creatorcontrib>Jeon, Kyeong-Bae</creatorcontrib><creatorcontrib>Poudel, Amrit</creatorcontrib><creatorcontrib>Lee, Hee Pom</creatorcontrib><creatorcontrib>Oh, Sei-Ryang</creatorcontrib><creatorcontrib>Ahn, Jongmin</creatorcontrib><creatorcontrib>Yoon, Do-Young</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Phytomedicine (Stuttgart)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Na-Yeon</au><au>Kim, Seonhwa</au><au>Park, Hyo-Min</au><au>Lim, Chae-Min</au><au>Kim, Jinju</au><au>Park, Jae-Young</au><au>Jeon, Kyeong-Bae</au><au>Poudel, Amrit</au><au>Lee, Hee Pom</au><au>Oh, Sei-Ryang</au><au>Ahn, Jongmin</au><au>Yoon, Do-Young</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cinnamomum verum extract inhibits NOX2/ROS and PKCδ/JNK/AP-1/NF-κB pathway-mediated inflammatory response in PMA-stimulated THP-1 monocytes</atitle><jtitle>Phytomedicine (Stuttgart)</jtitle><addtitle>Phytomedicine</addtitle><date>2023-04</date><risdate>2023</risdate><volume>112</volume><spage>154685</spage><epage>154685</epage><pages>154685-154685</pages><artnum>154685</artnum><issn>0944-7113</issn><eissn>1618-095X</eissn><abstract>Cinnamomum verum J. Presl (Cinnamon) is widely used in the food and pharmaceutical industries. C. verum exhibits various biological activities. However, it is unclear whether C. verum can inhibit NOX, a major source of ROS generation, and exert anti-inflammatory and antioxidant effects in PMA-stimulated THP-1 cells.
This study investigates the anti-inflammatory and antioxidant effects of C. verum in PMA-stimulated THP-1 cells.
The MeOH extract of C. verum was analyzed using UPLC-QTOF/MS. Anti-inflammatory and antioxidant effects of C. verum extract were examined by DCF-DA staining, immunofluorescence staining, RT-PCR, and immunoblotting in PMA-stimulated THP-1 cells.
C. verum and its components, cinnamic acid and coumarin, significantly attenuated the expression of IL-1β, IL-8, CCL5, and COX-2 in PMA-stimulated THP-1. C. verum decreased ROS levels via NOX2 downregulation, as well as ameliorated plasma membrane translocation of PKCδ and decreased JNK phosphorylation. Besides, C. verum suppressed the nuclear translocation of AP-1 and NF-κB, which modulates diverse pro-inflammatory genes.
C. verum effectively inhibits inflammation and oxidative stress during monocyte-macrophage differentiation and downregulates inflammatory mediators via NOX2/ROS and PKCδ/JNK/AP-1/NF-κB signaling.
[Display omitted]</abstract><cop>Germany</cop><pub>Elsevier GmbH</pub><pmid>36753827</pmid><doi>10.1016/j.phymed.2023.154685</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-9362-7384</orcidid><orcidid>https://orcid.org/0000-0003-4979-3702</orcidid><orcidid>https://orcid.org/0000-0002-0154-614X</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0944-7113 |
| ispartof | Phytomedicine (Stuttgart), 2023-04, Vol.112, p.154685-154685, Article 154685 |
| issn | 0944-7113 1618-095X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2834220087 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Anti-inflammation Anti-Inflammatory Agents - metabolism Anti-Inflammatory Agents - pharmacology Antioxidant antioxidants Antioxidants - pharmacology cinnamic acid Cinnamic acid, Coumarin Cinnamomum verum Cinnamomum zeylanicum Cinnamon coumarin fluorescent antibody technique immunoblotting inflammation interleukin-8 Lipopolysaccharides - pharmacology Monocytes NF-kappa B - metabolism oxidative stress phosphorylation plasma membrane Reactive Oxygen Species - metabolism Signal Transduction Transcription Factor AP-1 - metabolism |
| title | Cinnamomum verum extract inhibits NOX2/ROS and PKCδ/JNK/AP-1/NF-κB pathway-mediated inflammatory response in PMA-stimulated THP-1 monocytes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-29T04%3A13%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Cinnamomum%20verum%20extract%20inhibits%20NOX2/ROS%20and%20PKC%CE%B4/JNK/AP-1/NF-%CE%BAB%20pathway-mediated%20inflammatory%20response%20in%20PMA-stimulated%20THP-1%20monocytes&rft.jtitle=Phytomedicine%20(Stuttgart)&rft.au=Kim,%20Na-Yeon&rft.date=2023-04&rft.volume=112&rft.spage=154685&rft.epage=154685&rft.pages=154685-154685&rft.artnum=154685&rft.issn=0944-7113&rft.eissn=1618-095X&rft_id=info:doi/10.1016/j.phymed.2023.154685&rft_dat=%3Cproquest_cross%3E2774897565%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2774897565&rft_id=info:pmid/36753827&rft_els_id=S0944711323000454&rfr_iscdi=true |